Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,535,934 T→C I34T (ATC→ACC)  crr → glucose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,535,9340TC99.3% 547.5 / NA 152I34T (ATC→ACC) crrglucose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (80/70);  total (82/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 152 total aligned reads displayed.

CGCATTAAGAAGATTATCCGTAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTT  >  NC_000913/2535659‑2536202
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                             
   aTTAAGAAGATTATCCGTAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCg                                                                                                                                                                                                                                                                             <  3:202912/275‑1 (MQ=255)
     tAAGAAGATTATCCGTAACACGAACTGCGAAGATGCGAATGTGTTAGCAGAGCAGACTCTTGCTCAAACGACAACTAACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGTTTTCCGACGACAAGAAGAATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAAATCGTCAATACCGaa                                                                                                                                                                                                                                                                           <  1:55876/275‑1 (MQ=255)
             ttATCCGTAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGACCAATTTACTGCTTAGGTGCAGATCATGGGTTTGGTCGATAAACTGAAATCTTTGGCTTCCGACGACAAGAATGTGACCGGACTCTTGGTATAATTGTTCCGATGTCTGGCGCAGTCGTCATTACCAAGTCCGTGCCggtg                                                                                                                                                                                                                                                               >  4:376438/1‑276 (MQ=255)
                   gTAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTCAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTCCCCCGCTCTCCGGCCAGATCGTCAATACCTAACACGGGCCGGATGCCGt                                                                                                                                                                                                                                                          >  4:226780/1‑278 (MQ=255)
                      acacGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGCACTATTGAGATCATTGCTCCGCTCTCTGGCGGGATCGTCAATACCGAAGACGTGCCGGATGTCGtttt                                                                                                                                                                                                                                                       >  2:232041/1‑278 (MQ=255)
                          gAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACCAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTATGGCGAGATCGTCCATACCGAATACGTGCCGGATGTCGTTTTTGCg                                                                                                                                                                                                                                                   >  4:249009/1‑278 (MQ=255)
                            aCTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGc                                                                                                                                                                                                                                                    <  3:547225/275‑1 (MQ=255)
                            aCTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGa                                                                                                                                                                                                                                                 >  4:557174/1‑278 (MQ=255)
                            aCTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCCCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGAAGTCGTTTTGGc                                                                                                                                                                                                                                                    >  3:641182/1‑275 (MQ=255)
                                  aaGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAAGCTATGGATTCCGACGACAAGAAGGATAAGGGAACTATGGAGATCATTGCTCCGCTATCTGGCGAGAGAGGCAATACCGAAGAAGTGCCGGATGTCGTGGTTGCGGagaaaaa                                                                                                                                                                                                                                           >  2:590327/1‑277 (MQ=255)
                                    gATGCGAAGGGGTTAGCAGAGCATGCTCTTGCTCAAACGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCATCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCg                                                                                                                                                                                                                                         <  4:196487/278‑1 (MQ=255)
                                       gCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCg                                                                                                                                                                                                                                         <  1:533995/275‑1 (MQ=255)
                                        cGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGt                                                                                                                                                                                                                                                          >  3:192133/1‑257 (MQ=255)
                                        cGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGt                                                                                                                                                                                                                                        <  1:644406/275‑1 (MQ=255)
                                         gAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGt                                                                                                                                                                                                                                    <  2:17019/278‑1 (MQ=255)
                                          aagtagctAGCAGAGGAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTAATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCAGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGt                                                                                                                                                                                                                                    <  2:65749/270‑1 (MQ=255)
                                           aGGTGTTAGCAGAGCAGGCTCGTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGa                                                                                                                                                                                                                                  <  2:44818/278‑1 (MQ=255)
                                           aGGTGTTAGCACAGCAGGCACATGCTCAACCGACAACGAACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGGGAGGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGa                                                                                                                                                                                                                                  <  4:205286/278‑1 (MQ=255)
                                            ggTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGt                                                                                                                                                                                                                                    <  1:198561/275‑1 (MQ=255)
                                                       agcagGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATCCCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGc                                                                                                                                                                                                                         >  3:666958/1‑275 (MQ=255)
                                                            gCTTTTGGTCAACCGACAACGGGCGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATc                                                                                                                                                                                                                     <  1:332535/274‑1 (MQ=255)
                                                            gCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAAACGTGCCGGATGTCGTTTTTGAGGAAAAGAGCTTTGGTGATGGTATAGCTagaaaaa                                                                                                                                                                                                                 >  4:487507/1‑272 (MQ=255)
                                                                        ccGAGAACGGACGGATTAATGACGCAGGTTAACAAGTTCATTGAAAAAAAAACAATCGGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGt                                                                                                                                                                                                         <  3:424401/274‑1 (MQ=255)
                                                                               cGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGt                                                                                                                                                                                              <  2:557174/278‑1 (MQ=255)
                                                                               cGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGt                                                                                                                                                                                              <  2:487507/278‑1 (MQ=255)
                                                                                 gaaaggTTAATGAAGCTGGGTAACAAGTTCATTGAAGAAAAAACAATCTGCTAAACCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTcg                                                                                                                                                                                            <  2:477548/273‑1 (MQ=255)
                                                                                  aCGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGTGTGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCTAAACAACGGGGAAAAAAAAGGt                                                                                                                                                                                              >  3:399111/1‑275 (MQ=255)
                                                                                      gTTAATGACTCTGGTTAACAAGTTCATTGAATAAAAAACAATCTGCTAATCGACGAGATGCGGCCCTATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCa                                                                                                                                                                                       <  4:399679/278‑1 (MQ=255)
                                                                                                   gTTAAGAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGGGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTACCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCa                                                                                                                                                                         <  2:331632/278‑1 (MQ=255)
                                                                                                   gTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGAGAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCa                                                                                                                                                                         <  4:286094/278‑1 (MQ=255)
                                                                                                             tCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGTTGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTaa                                                                                                                                                                  >  3:64921/1‑275 (MQ=255)
                                                                                                                  gaagaaAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGa                                                                                                                                                                                                                                  <  2:109149/207‑1 (MQ=255)
                                                                                                                             aaTCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAaaccaac                                                                                                                                                  <  1:666959/275‑1 (MQ=255)
                                                                                                                               tCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCTCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGTTAACAAAATGGTCGCGCCAGTAGACTGCACCATTGGTAAAATCTTTAAAACCAAACACg                                                                                                                                              >  4:612295/1‑277 (MQ=255)
                                                                                                                                  gCTAATCCACTCGATGCTGCCCAATTTACTGCGTAGGAGAAGATCATGGGTTTGTTCGATAAACTGGAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGc                                                                                                                                             <  3:717464/275‑1 (MQ=255)
                                                                                                                                  gCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAATATCATGGGTGTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGATATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTTCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCAtt                                                                                                                                          <  2:189985/278‑1 (MQ=255)
                                                                                                                                     aaTCCACGAGATGCGGCCACATTTAAGACTTAGGAGAAGATCATGGATTGTGTCGATAAACTGGAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTAGTGAGCTCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAATACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAGCAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCATTCACGCAtt                                                                                                                                          <  3:91246/275‑1 (MQ=255)
                                                                                                                                           cGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTAt                                                                                                                                    >  1:506722/1‑275 (MQ=255)
                                                                                                                                           cGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCCCTATCGa                                                                                                                                 >  2:270783/1‑278 (MQ=255)
                                                                                                                                            ggtagtgtgCCCAATTCCTTGCTTAGAAGAATATCATTGGTTTGTTGACTAAACTGAAAGCTCGTGTATCCGGCGACAAGAAAGCGACCGGAACTATAGTGATCATTACTCCGCTCTCTTGCGAGATCGTCAATACCGAGGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGGTGGTTATGGTATTGCCATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATc                                                                                                                                   <  3:197962/267‑1 (MQ=255)
                                                                                                                                                  cGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCTGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGt                                                                                                                                                                    >  3:586737/1‑236 (MQ=255)
                                                                                                                                                  cGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATAGAATCTGAt                                                                                                                          >  2:601400/1‑278 (MQ=255)
                                                                                                                                                      ccAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCTGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTaaaa                                                                                                                                                                <  1:586738/236‑1 (MQ=255)
                                                                                                                                                       cAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTAAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg                                                                                                                        <  3:360162/275‑1 (MQ=255)
                                                                                                                                                          tttACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGt                                                                                                                  >  2:349893/1‑278 (MQ=255)
                                                                                                                                                               tGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTCTTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAACAGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCCCTATCGAATCTCCAAGCGGCTTTTAAc                                                                                                             >  4:121303/1‑278 (MQ=255)
                                                                                                                                                               tGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTTAAACCAACCACGCATTCTCTAACGAATCTGATAGCGGCGt                                                                                                                  >  1:256287/1‑273 (MQ=255)
                                                                                                                                                               tGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTTAAACCAAAACCGCATTCCCTATCCAATCTGATAGCGGCGtta                                                                                                                >  1:388227/1‑274 (MQ=255)
                                                                                                                                                                    atgagaAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGGAACAAAATGGTGGCGACAGTAGACGGCACCCTTGGTCAAATCTTGGCAACCAAACACCCATTCTCTAGACAATCTGAACACGGTCTTGAACTCTTc                                                                                                        >  4:166786/3‑278 (MQ=255)
                                                                                                                                                                    aGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTTCAGAAAAAATCGTTGGTGATGGGATTGCTACTAAGCGAACGGGTAATAAAATGGTCGCGCCAGTAGGCGGACACCTTGTGAAAATCGTTGAAACCAACCACCACTTCTCTACTGAAACTGAAAGCGGCGTTTaatgt                                                                                                           >  3:92187/1‑272 (MQ=255)
                                                                                                                                                                           gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCTCCAGTGGACGGCACCATTGGGAAAATCTTTGAAACCAACAACGCATTCACTATCGAATCTGAAAGCAGCGTTTAACTTTTCGTCCACt                                                                                                 >  4:563134/1‑278 (MQ=255)
                                                                                                                                                                           gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCACCCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACt                                                                                                 >  2:206235/1‑278 (MQ=255)
                                                                                                                                                                           gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTcc                                                                                                    >  1:646779/1‑275 (MQ=255)
                                                                                                                                                                           gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACt                                                                                                 >  2:407120/1‑278 (MQ=255)
                                                                                                                                                                            aTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTAt                                                                                                                                                                                                                      <  2:610557/161‑1 (MQ=255)
                                                                                                                                                                              cATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCg                                                                                              <  2:36708/278‑1 (MQ=255)
                                                                                                                                                                                tGGGTTTGTTCGATAGACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATAGCTCCGCTCTCTGGCGCGATCGTCACTACCGAATACGTGCCGGATGTCGTTTTTGCGGAATAAATCGTTGGTGATGGTATTGCTATCCAACCAACGGGCAACAACATGGTCGTGCCCGTAGACGGCACCCTTGGTAAACCTTTTGAAATCAACAACGCTTTCTCCTCCGACTCTGACAGCGACGTTTACCTGTTCGACCATTTc                                                                                               >  3:685297/1‑275 (MQ=255)
                                                                                                                                                                                tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACggg                                                                                                                                                                                                          >  2:146111/1‑169 (MQ=255)
                                                                                                                                                                                tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTc                                                                                               >  1:230381/1‑275 (MQ=255)
                                                                                                                                                                                tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCAACATTGGTAAAATCTTTGAAACGAACCAAGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTc                                                                                               >  1:170165/1‑275 (MQ=255)
                                                                                                                                                                                tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCAACATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGCACTGTTCGTCCACTTCGGt                                                                                            >  2:187599/1‑278 (MQ=255)
                                                                                                                                                                                tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGACGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACAACGCCTTCTTTATCGAATCTGATCGCGGTGTTGAACTGTTCGTCCAATTc                                                                                               >  1:674360/1‑275 (MQ=255)
                                                                                                                                                                                 ggggTTGTTAGATAAACTGAAAGCTCTGGTTTCCGACGACAAGAAGGGTACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGt                                                                                                      <  2:607747/267‑1 (MQ=255)
                                                                                                                                                                                 gggTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGt                                                                                                                                                                                                         <  4:146111/169‑1 (MQ=255)
                                                                                                                                                                                  ggTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGt                                                                                            <  2:179833/276‑1 (MQ=255)
                                                                                                                                                                                      tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTGGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACAACGCATTCTCTATCGAATCTGATAGCGACGTTGAATTGTTCGTCCACTTCGGt                                                                                            >  1:164761/1‑272 (MQ=255)
                                                                                                                                                                                      tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGTCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTCACGAATCTGATAGCGGCCTTGACCAGTTCGTCCCCTTCgct                                                                                            >  1:206573/1‑270 (MQ=255)
                                                                                                                                                                                      tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCCCTATCGAATCTGATAGCGGCGTTTAACTGTTCTTCCACTTCGGTATCGa                                                                                       >  4:308862/1‑277 (MQ=255)
                                                                                                                                                                                        ttCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa                                                                                       <  1:405781/275‑1 (MQ=255)
                                                                                                                                                                                          cGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGaca                                                                                     <  3:230380/275‑1 (MQ=255)
                                                                                                                                                                                             tAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGGTACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGCAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAACATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCTTTg                                                                               >  2:246621/1‑278 (MQ=255)
                                                                                                                                                                                               aaCTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGtt                                                                                <  1:2664/275‑1 (MQ=255)
                                                                                                                                                                                                  tGAAAACTCTGGTTTCCGAAGAAAAGAAGGATACCGGAACTATTGAGATCATTGTTCCGCTCTCTGGCGGTATCGTCACTACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGATATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAGCCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGaa                                                                             <  3:285044/275‑1 (MQ=255)
                                                                                                                                                                                                           tGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGTATGTCGTTTTTGCGGAAACAATCGTTCGTGACGGTATGGCTATCCACCCAACGTGTAACAACATGGTACAGCCAGTACACTGCAACACTGTTAAACTCTTTGAAAGCAAACACCCATTCCCCATCGAATCTGCAAGAGCCTTTTAACTATTCGCCCACTTCTGTATCCACACCCTTAACCTCAAAAGc                                                                    >  3:657471/1‑275 (MQ=255)
                                                                                                                                                                                                           tGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCATACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGGCCACTTCGGTATCGACACCGTTGAACTGACAGGGGaa                                                                 >  4:255574/1‑278 (MQ=255)
                                                                                                                                                                                                              tttCCGACGAAAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGaa                                                                 <  1:79427/275‑1 (MQ=255)
                                                                                                                                                                                                                   gacgacAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTAAAAGCGGCGTTGAACTGTTCGCCCACTTCGGTATCGCCACCGTTGAACTGAAAGGAGAAGGCTTCaa                                                         >  4:139473/1‑278 (MQ=255)
                                                                                                                                                                                                                                 tACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATc                                                                                         >  3:119307/1‑232 (MQ=255)
                                                                                                                                                                                                                                 tACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTgaag                                           >  2:545246/1‑278 (MQ=255)
                                                                                                                                                                                                                                  aCCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTgaaga                                          >  2:129005/1‑278 (MQ=255)
                                                                                                                                                                                                                                     ggAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGaca                                                                                     <  1:119308/232‑1 (MQ=255)
                                                                                                                                                                                                                                      gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTgaagaa                                         >  1:416175/1‑275 (MQ=255)
                                                                                                                                                                                                                                      gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTATATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGAGAAGGCTTCAAGCGTATTGCTgaagaa                                         >  1:652763/1‑275 (MQ=255)
                                                                                                                                                                                                                                            ttGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCa                                                          >  3:15740/1‑252 (MQ=255)
                                                                                                                                                                                                                                            ttGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCCACACCGTTGAACTGCAAGGCGAAGGCGTCAAGCGTATTGCTGAAGAAGGTCAgcga                                >  4:497565/1‑277 (MQ=255)
                                                                                                                                                                                                                                                 aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTa                                                                                           >  1:500922/1‑214 (MQ=255)
                                                                                                                                                                                                                                                   cATTGCGCCGGTCTCTGGCGAGATCGTCAATACCGAAGACTTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAg                         <  4:601400/278‑1 (MQ=255)
                                                                                                                                                                                                                                                    aTTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGcgcg                               >  1:593257/1‑271 (MQ=255)
                                                                                                                                                                                                                                                    aTTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGcgcg                               >  1:453543/1‑271 (MQ=255)
                                                                                                                                                                                                                                                    aTTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGaa                           >  3:336147/1‑275 (MQ=255)
                                                                                                                                                                                                                                                    aTTGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCGCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACTCCGTTGAACTGAAAGGCGACGGCTTCAAGCGTATTGCTTAAGAAGGTCAGCGCGTGAGAGt                        >  4:188592/1‑278 (MQ=255)
                                                                                                                                                                                                                                                     ttGCTCCGCTCTCTGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa                                                                                       <  3:500921/214‑1 (MQ=255)
                                                                                                                                                                                                                                                             ctctctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAACATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGTTGAAGAAGGTCAGCGCGTGCACGTCGGCGATAc               >  2:71173/1‑278 (MQ=255)
                                                                                                                                                                                                                                                             ctctctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGa                  >  1:124747/1‑275 (MQ=255)
                                                                                                                                                                                                                                                             ctctctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTATCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCATCTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGa                  >  1:227647/1‑275 (MQ=255)
                                                                                                                                                                                                                                                              tctctGGCGAGATCGTCAAGACCGAAGACGGGCCGGATGTCGTTTTGGCGGAAAAAATCGTTGGTGATGGTATTTCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGa                  <  1:297150/274‑1 (MQ=255)
                                                                                                                                                                                                                                                               ctctGGCGAGATCGTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATa                <  3:705606/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                    acgtaaTCGTCAAAACCGAAGACGGGCCTGTTGTCGGTTTTGCGGAAAAAATCGTGGGTGATGTTATGGCTATCAAACCAACGGGTAACAAAATGGGCGCTCCAGTAGACGGGACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCAt         <  4:642080/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aTCGTCAATACCGAAGACGTGCCGGATGTCGTTTTGGAGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAt    <  2:253580/277‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gTCAATACCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCACGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAttt  >  2:217083/1‑276 (MQ=255)
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                             
CGCATTAAGAAGATTATCCGTAACACGAACTTCGAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTT  >  NC_000913/2535659‑2536202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: