Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,987,442 Δ1 bp 5.5% intergenic (+14/+65) ftnB → / ← yecJ ferritin B, putative ferrous iron reservoir/DUF2766 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,987,4420T. 5.5% 632.8 / 26.9 183intergenic (+14/+65)ftnB/yecJferritin B, putative ferrous iron reservoir/DUF2766 family protein
Reads supporting (aligned to +/- strand):  ref base T (83/89);  new base . (7/3);  total (90/93)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

Only 100 of 187 total aligned reads displayed.

GAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGC  >  NC_000913/1987294‑1987590
                                                                                                                                                    |                                                                                                                                                     
 aaaaaGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTa                                                                                                                                                      >  3:576522/1‑149 (MQ=255)
  aaaaGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTaa                                                                                                                                                     >  3:635276/1‑149 (MQ=255)
    aaGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATg                                                                                                                                                   <  3:534905/149‑1 (MQ=255)
      gAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCa                                                                                                                                                 >  2:990616/1‑149 (MQ=255)
      gAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCa                                                                                                                                                 <  2:281134/149‑1 (MQ=255)
           gcagcaTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTgcg                                                                                                                                           >  4:68836/1‑149 (MQ=255)
            cagcaTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTgcgc                                                                                                                                          <  1:1145089/149‑1 (MQ=255)
                atgatgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGAt                                                                                                                                      >  1:1213114/1‑149 (MQ=255)
                  gatgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGa                                                                                                                                    >  2:708335/1‑149 (MQ=255)
                   atgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGaa                                                                                                                                   <  3:555751/149‑1 (MQ=255)
                    tgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAg                                                                                                                                  >  3:276781/1‑149 (MQ=255)
                         tGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGtttt                                                                                                                             <  3:1351741/149‑1 (MQ=255)
                           ttGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTga                                                                                                                           <  1:1067319/149‑1 (MQ=255)
                           ttGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTga                                                                                                                           <  2:1514253/149‑1 (MQ=255)
                           ttGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTga                                                                                                                           <  2:1140237/149‑1 (MQ=255)
                           ttGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTga                                                                                                                           <  1:1103615/149‑1 (MQ=255)
                               tgcAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATg                                                                                                                                     >  1:183510/1‑135 (MQ=255)
                               tgcAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGaaa                                                                                                                       >  2:418477/1‑149 (MQ=255)
                                gcAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAAc                                                                                                                      >  4:482813/1‑149 (MQ=255)
                                   aaCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGc                                                                                                                   >  3:1264923/1‑149 (MQ=255)
                                           ttGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCAt                                                                                                           >  4:285794/1‑149 (MQ=255)
                                              atgaAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTg                                                                                                        >  4:413075/1‑149 (MQ=255)
                                                 aaGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGttt                                                                                                     <  3:1124488/149‑1 (MQ=255)
                                                    tGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGaa                                                                                                  <  1:990616/149‑1 (MQ=255)
                                                        cAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagcag                                                                                              <  2:387121/149‑1 (MQ=255)
                                                          gTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCg                                                                                            >  1:339428/1‑149 (MQ=255)
                                                           tGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGaa                                                                                                  <  1:949685/142‑1 (MQ=255)
                                                           tGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGaa                                                                                                  >  2:949685/1‑142 (MQ=255)
                                                           tGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCgg                                                                                           >  1:592696/1‑149 (MQ=255)
                                                            gCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGt                                                                                          <  1:685315/149‑1 (MQ=255)
                                                             cGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGtt                                                                                                      <  2:1350107/136‑1 (MQ=255)
                                                             cGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtt                                                                                         >  1:1354828/1‑149 (MQ=255)
                                                              gAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGaa                                                                                                  >  2:730982/1‑139 (MQ=255)
                                                              gAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGaa                                                                                                  <  1:730982/139‑1 (MQ=255)
                                                              gAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGttt                                                                                        <  4:1086029/149‑1 (MQ=255)
                                                              gAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGttt                                                                                        <  4:10699/149‑1 (MQ=255)
                                                               aaaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtttt                                                                                       <  4:348211/149‑1 (MQ=255)
                                                               aaaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtttt                                                                                       >  1:852369/1‑149 (MQ=255)
                                                                      cGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAtt                                                                                <  2:143793/149‑2 (MQ=255)
                                                                      cGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATg                                                                                <  3:395908/149‑1 (MQ=255)
                                                                         gTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGa                                                                             <  3:84880/149‑1 (MQ=255)
                                                                            tgtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTc                                                                          >  2:1057544/1‑149 (MQ=255)
                                                                                  cTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCtg                                                                    >  3:596479/1‑149 (MQ=255)
                                                                                      gCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTggg                                                                <  4:596479/149‑1 (MQ=255)
                                                                                      gCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTggg                                                                <  4:256580/149‑1 (MQ=255)
                                                                                        aGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTgg                                                                 <  2:929377/146‑1 (MQ=255)
                                                                                        aGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTgg                                                                 >  1:929377/1‑146 (MQ=255)
                                                                                        aGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGt                                                              >  1:498682/1‑149 (MQ=255)
                                                                                         gaccgaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTa                                                             >  3:1144501/1‑149 (MQ=255)
                                                                                             gaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt                                                                                 >  1:477148/1‑125 (MQ=255)
                                                                                             gaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt                                                                                 <  2:477148/125‑1 (MQ=255)
                                                                                              accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAtt                                                                           >  2:189835/1‑130 (MQ=255)
                                                                                              accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACtt                                                        >  4:1198111/1‑149 (MQ=255)
                                                                                              accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACtt                                                        >  1:863979/1‑149 (MQ=255)
                                                                                              accAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACtt                                                        <  1:134314/149‑1 (MQ=255)
                                                                                                cAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                      <  4:1431892/149‑1 (MQ=255)
                                                                                                cAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                      <  2:278539/149‑1 (MQ=255)
                                                                                                cAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                      >  3:129514/1‑149 (MQ=255)
                                                                                                cAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                      <  4:576522/149‑1 (MQ=255)
                                                                                                cAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                      >  1:278539/1‑149 (MQ=255)
                                                                                                cAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                      <  1:1454938/149‑1 (MQ=255)
                                                                                                 aaCATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAg                                                     <  2:1209847/149‑1 (MQ=255)
                                                                                                  aCATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGt                                                    >  1:211856/1‑149 (MQ=255)
                                                                                                  aCATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGt                                                    >  3:199377/1‑149 (MQ=255)
                                                                                                     tGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCa                                                 >  1:458838/1‑149 (MQ=255)
                                                                                                      gTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCaa                                                <  2:56546/149‑1 (MQ=255)
                                                                                                       ttCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGttt                                                                                                     >  4:16385/1‑95 (MQ=255)
                                                                                                         cTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATaa                                             >  3:253330/1‑149 (MQ=255)
                                                                                                          tGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAg                                            >  4:945374/1‑149 (MQ=255)
                                                                                                          tGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGAGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATACACCCTGTGGGGTAAACTTGAGATCAATAAg                                            >  3:1240773/1‑149 (MQ=255)
                                                                                                           gAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa                                                      >  1:774042/1‑138 (MQ=255)
                                                                                                            aaTGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAgcg                                          >  3:377055/1‑149 (MQ=255)
                                                                                                                    gtCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAgcg                                          <  4:660559/141‑1 (MQ=255)
                                                                                                                       acacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCtttt                               >  3:670310/1‑149 (MQ=255)
                                                                                                                        cacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCttttt                              <  2:744096/149‑1 (MQ=255)
                                                                                                                        cacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCAGCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGTGTAAAATTGAGTTCAATAAGCGAGATTGCttgtt                              <  4:1433589/149‑1 (MQ=255)
                                                                                                                         acCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTg                             >  4:632380/1‑149 (MQ=255)
                                                                                                                         acCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTg                             >  2:544366/1‑149 (MQ=255)
                                                                                                                            aGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTc                                                  <  3:792978/125‑1 (MQ=255)
                                                                                                                            aGCTGCATTGATCATCATAGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTc                                                  >  4:792978/1‑125 (MQ=255)
                                                                                                                            aGCGGCATTGATCATCATCGGCGCTAATTCATTGCGCCGATGAAGGTTTTGAGAAACAGCTGCCTCATCTGTTTGATGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCTATAAGCGCGATGGCTTTTTGGAt                          <  4:276781/149‑1 (MQ=255)
                                                                                                                             gCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCCTCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGAtt                         <  2:1127792/149‑1 (MQ=255)
                                                                                                                                gCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGcc                      <  2:311332/149‑1 (MQ=255)
                                                                                                                                gCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCCGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGcc                      <  2:842518/149‑1 (MQ=255)
                                                                                                                                  aTTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCg                    >  2:139177/1‑149 (MQ=255)
                                                                                                                                     gATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCAt                 <  3:438524/149‑1 (MQ=255)
                                                                                                                                      atcatcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCt                                                                                                         <  4:97491/60‑1 (MQ=255)
                                                                                                                                      atcatcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCt                                                                                                         >  3:97491/1‑60 (MQ=255)
                                                                                                                                        catcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTaa                                                                                  <  2:1089164/81‑1 (MQ=255)
                                                                                                                                        catcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTaa                                                                                  >  1:1089164/1‑81 (MQ=255)
                                                                                                                                           catcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGAc           >  4:388399/1‑149 (MQ=255)
                                                                                                                                           catcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGAc           >  4:1150742/1‑149 (MQ=255)
                                                                                                                                            atcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATgg                                                                               >  2:1114260/1‑80 (MQ=38)
                                                                                                                                             tcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCg         >  2:1039900/1‑149 (MQ=255)
                                                                                                                                             tcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCg         >  4:1272745/1‑149 (MQ=255)
                                                                                                                                                gcgcTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGt      >  1:1055602/1‑149 (MQ=255)
                                                                                                                                                  gcTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCt    >  1:390372/1‑149 (MQ=255)
                                                                                                                                                   cTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTg   <  3:1462272/149‑1 (MQ=255)
                                                                                                                                                    tAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgc  <  3:489622/149‑1 (MQ=255)
                                                                                                                                                    tAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGCTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgc  >  4:537837/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGC  >  NC_000913/1987294‑1987590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: