Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,552,837 Δ1 bp 5.2% intergenic (+17/‑64) uxuA → / → uxuB mannonate hydrolase/D‑mannonate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,552,8370T. 5.2% 603.9 / 23.9 172intergenic (+17/‑64)uxuA/uxuBmannonate hydrolase/D‑mannonate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (90/73);  new base . (4/5);  total (94/78)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 174 total aligned reads displayed.

GGTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCG  >  NC_000913/4552692‑4552985
                                                                                                                                                 |                                                                                                                                                     
ggTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTgcg                                                                                                                                                    >  3:214503/1‑149 (MQ=255)
ggTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTgcg                                                                                                                                                    >  4:156354/1‑149 (MQ=255)
 gTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTgcgc                                                                                                                                                   >  1:96056/1‑149 (MQ=255)
   catcaGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTgcgcgc                                                                                                                                                >  3:1221587/1‑149 (MQ=255)
     tcaGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCg                                                                                                                                              >  4:915107/1‑149 (MQ=255)
     tcaGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCg                                                                                                                                              >  1:1447990/1‑149 (MQ=255)
        gATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGc                                                                                                                                           <  3:966315/149‑1 (MQ=255)
         aTGCTGGACGACCTGAAGAAGACAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGcc                                                                                                                                          >  4:1429997/1‑149 (MQ=255)
            cTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCACGGCGCGCAGCCATGCCGGt                                                                                                                                       <  3:817621/149‑1 (MQ=255)
             tGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCACGGCGCGCAGCCATGCCGGtg                                                                                                                                      <  2:1001326/149‑2 (MQ=255)
              ggACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTc                                                                                                                                     <  3:733991/149‑1 (MQ=255)
               gacgacCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTcc                                                                                                                                    >  4:1109144/1‑149 (MQ=255)
               gacgacCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTcc                                                                                                                                    <  1:295234/149‑1 (MQ=255)
               gacgacCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTcc                                                                                                                                    <  1:23337/149‑1 (MQ=255)
                     cTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCttcttc                                                                                                                              <  4:514915/149‑1 (MQ=255)
                     cTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCttcttc                                                                                                                              >  3:652370/1‑149 (MQ=255)
                     cTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCACGGCGCGCAGCCATGCCGGt                                                                                                                                       <  4:574735/140‑1 (MQ=255)
                     cTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCACGGCGCGCAGCCATGCCGGt                                                                                                                                       >  3:574735/1‑140 (MQ=255)
                        aagaagaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCtt                                                                                                                           <  3:636840/149‑1 (MQ=255)
                         agaagaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTg                                                                                                                          >  3:360117/1‑149 (MQ=255)
                          gaagaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGc                                                                                                                         >  1:443860/1‑149 (MQ=255)
                          gaagaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGc                                                                                                                         >  1:1179729/1‑149 (MQ=255)
                             gaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGt                                                                                                                      >  2:38970/1‑149 (MQ=255)
                                aaccaaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGTCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCAc                                                                                                                   >  3:917930/1‑149 (MQ=255)
                                aaccaaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCAc                                                                                                                   >  3:1183330/1‑149 (MQ=255)
                                aaccaaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCAc                                                                                                                   >  1:651291/1‑149 (MQ=255)
                                  ccaaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCActc                                                                                                                 >  1:247792/1‑149 (MQ=255)
                                   caaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCActct                                                                                                                >  4:1029243/1‑149 (MQ=255)
                                    aaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCtt                                                                                                               >  1:454257/1‑149 (MQ=255)
                                    aaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCtt                                                                                                               <  2:984868/149‑1 (MQ=255)
                                     accCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCttt                                                                                                              <  4:652370/149‑1 (MQ=255)
                                     accCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCttt                                                                                                              <  2:1447990/149‑1 (MQ=255)
                                       ccAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGa                                                                                                            <  1:747513/149‑1 (MQ=255)
                                         aGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAg                                                                                                          >  1:355985/1‑149 (MQ=255)
                                                tCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGAtt                                                                                                   >  4:364909/1‑149 (MQ=255)
                                                tCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGAtt                                                                                                   <  1:1026075/149‑1 (MQ=255)
                                                tCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGAtt                                                                                                   >  4:1142662/1‑149 (MQ=255)
                                                tCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGAtt                                                                                                   >  3:1029462/1‑149 (MQ=255)
                                                tCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGAGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGTCGTCACTCTTTGAAGACGGAtt                                                                                                   >  4:1222233/1‑149 (MQ=255)
                                                     aaTTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGa                                                                                              <  2:862538/149‑1 (MQ=255)
                                                        tGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGCCGATCCAGCGCGCTTTCTTTAGCCGGTAATATCAACCGGCATGGCTGCGTGCCATGCCGGTTCCTTCTTCCTTGCCGTCACTCGTTGAAGACGGATTCTGGACtt                                                                                           <  4:921345/149‑1 (MQ=255)
                                                             gtcTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGa                                                                                      >  3:299670/1‑149 (MQ=255)
                                                             gtcTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGa                                                                                      <  4:440417/149‑1 (MQ=255)
                                                             gtcTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGa                                                                                      <  4:741323/149‑1 (MQ=255)
                                                              tcTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTGAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGAt                                                                                     <  1:1399718/149‑1 (MQ=255)
                                                               cTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGt                                                                                                                                             >  4:776690/1‑92 (MQ=255)
                                                                tGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTg                                                                                                >  3:1430700/1‑136 (MQ=255)
                                                                tGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGa                                                                                   >  4:662908/1‑149 (MQ=255)
                                                                 gAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGac                                                                                  >  2:149563/1‑149 (MQ=255)
                                                                   aaGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGTTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGacta                                                                                <  4:23397/149‑1 (MQ=255)
                                                                    aGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGactac                                                                               <  4:482935/149‑1 (MQ=255)
                                                                      gCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGActacta                                                                             <  2:1210233/149‑1 (MQ=255)
                                                                        cTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTAtt                                                                           <  1:1094956/149‑1 (MQ=255)
                                                                           gCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTAttgtt                                                                        <  2:247792/149‑1 (MQ=255)
                                                                           gCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTAttgtt                                                                        <  2:1456395/149‑1 (MQ=255)
                                                                             cGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGa                                                                      <  1:1370431/149‑1 (MQ=255)
                                                                              gAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTAttgtt                                                                        >  2:932828/1‑146 (MQ=255)
                                                                              gAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGAc                                                                     >  1:856467/1‑149 (MQ=255)
                                                                                aGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAg                                                                   <  4:286235/149‑1 (MQ=255)
                                                                                   tCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCaa                                                                <  2:982019/149‑1 (MQ=255)
                                                                                    cgcgGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAAt                                                               <  2:359764/149‑1 (MQ=255)
                                                                                         tgtCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGcc                                                          <  4:768944/149‑1 (MQ=255)
                                                                                             gAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGtt                                                      >  4:725278/1‑149 (MQ=255)
                                                                                              aaCTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTg                                                     >  2:1255592/1‑149 (MQ=255)
                                                                                                   gCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTgcccgc                                                >  3:931339/1‑149 (MQ=255)
                                                                                                     gATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCa                                                                 >  1:627055/1‑130 (MQ=255)
                                                                                                     gATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCa                                                                 <  2:627055/130‑1 (MQ=255)
                                                                                                     gATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTgcccgccc                                              >  4:339245/1‑149 (MQ=255)
                                                                                                     gATCCAGCGCGCGTTCTTTAGGCGTGAAGATCGACGGGGATGGCTGCGCGCCGTGCCGCTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGGTGACAGCAATCTGCCGGTTgcccgccc                                              <  2:923737/149‑1 (MQ=255)
                                                                                                          aGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCAt                                         <  1:898629/149‑1 (MQ=255)
                                                                                                           gcgcgcTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATg                                        >  2:171949/1‑149 (MQ=255)
                                                                                                                 tttctttAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCa                                  >  1:53466/1‑149 (MQ=255)
                                                                                                                 tttctttAGCCGTCAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACa                                                                    <  1:1035662/115‑1 (MQ=255)
                                                                                                                 tttctttAGCCGTCAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACa                                                                    >  2:1035662/1‑115 (MQ=255)
                                                                                                                  ttctttAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCAt                                 <  3:683389/149‑1 (MQ=255)
                                                                                                                     tttAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATtct                              >  2:172974/1‑149 (MQ=255)
                                                                                                                     tttAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATtct                              >  3:456484/1‑149 (MQ=255)
                                                                                                                     tttAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATtct                              >  1:761818/1‑149 (MQ=255)
                                                                                                                          ccGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCt                         <  2:85573/149‑1 (MQ=255)
                                                                                                                          ccGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCt                         >  3:370319/1‑149 (MQ=255)
                                                                                                                           cGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTg                        >  2:845070/1‑149 (MQ=255)
                                                                                                                             ttAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGa                      >  3:1083704/1‑149 (MQ=255)
                                                                                                                                atatCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAGTc                   <  3:496468/149‑1 (MQ=255)
                                                                                                                                  atCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGa                                                                      >  1:1494772/1‑96 (MQ=255)
                                                                                                                                  atCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCAc                 >  1:18835/1‑149 (MQ=255)
                                                                                                                                   tCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACg                >  2:1433375/1‑149 (MQ=255)
                                                                                                                                    ccACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGc               <  3:148058/149‑1 (MQ=255)
                                                                                                                                     aaCCGGCACGGCGCGCAGCCATGCCGGTTCCTTCTTCCTTGCCGTCTCTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCa              >  2:1281481/2‑149 (MQ=255)
                                                                                                                                      aCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCAt             >  2:1378327/1‑149 (MQ=255)
                                                                                                                                      aCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCAt             >  2:1350507/1‑149 (MQ=255)
                                                                                                                                      aCCGGCACGGCGCGCAGCCATGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCAt                                         >  1:1316262/1‑121 (MQ=255)
                                                                                                                                      aCCGGCACGGCGCGCAGCCATGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCAt             >  1:596372/1‑149 (MQ=255)
                                                                                                                                       ccGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCAtt            <  2:454257/149‑1 (MQ=255)
                                                                                                                                       ccGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCAtt            <  1:1213575/149‑1 (MQ=255)
                                                                                                                                          gCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATtgtg         <  3:7491/149‑1 (MQ=255)
                                                                                                                                            aTGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCa       >  1:819425/1‑149 (MQ=255)
                                                                                                                                            aTGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCa       <  4:1029462/149‑1 (MQ=255)
                                                                                                                                             tGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCACCCGTCATgg                                       >  4:1324785/1‑116 (MQ=255)
                                                                                                                                             tGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCACCCGTCATgg                                       <  3:1324785/116‑1 (MQ=255)
                                                                                                                                                 tGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCg  >  3:906438/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GGTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCG  >  NC_000913/4552692‑4552985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: