Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,524,463 | Δ1 bp | 5.5% | intergenic (+95/+18) | yncE → / ← ansP | ATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,524,463 | 0 | A | . | 5.5% | 563.2 / 23.3 | 163 | intergenic (+95/+18) | yncE/ansP | ATP‑binding protein, periplasmic, function unknown/L‑asparagine transporter |
Reads supporting (aligned to +/- strand): ref base A (83/71); new base . (3/6); total (86/77) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.09e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 |
Only 100 of 164 total aligned reads displayed.
CTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGA > NC_000913/1524316‑1524611 | cTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGACTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAg > 4:1403421/1‑149 (MQ=255) tAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGc > 1:826746/1‑149 (MQ=255) aaCAGCAGTAAGCTATCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCg < 2:390412/149‑1 (MQ=255) aaCAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCg < 4:1000462/149‑1 (MQ=255) agGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGT‑GCCGGGCaaa < 2:1103500/149‑2 (MQ=255) aaGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGT‑GCCGGGCAAattc < 4:889216/149‑2 (MQ=255) aGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGT‑GCCGGGCAAattca < 2:384378/149‑3 (MQ=255) aGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAAtttt < 4:469465/148‑1 (MQ=255) gCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGCTACGT‑GCCGGGCaaattcag < 1:1341974/149‑7 (MQ=255) gCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGc < 4:832084/149‑1 (MQ=255) tACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCaa < 1:85130/149‑1 (MQ=255) cccAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAAtt < 3:1066994/149‑1 (MQ=255) cccAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAAtt < 4:1178505/149‑1 (MQ=255) cccAGCCAGACGATGTGATTCGTATTCCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAAtt > 3:1244237/1‑149 (MQ=255) gccagACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTaa < 4:297988/147‑1 (MQ=255) gccagACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACt > 2:1374811/1‑149 (MQ=255) ccagACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTc < 2:150705/149‑1 (MQ=255) gACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGcc < 4:16516/149‑1 (MQ=255) gATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGtt > 3:8352/1‑149 (MQ=255) gATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGtt > 2:1065166/1‑149 (MQ=255) aTGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGttt > 1:1064706/1‑149 (MQ=255) tgtgATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTc > 2:464120/1‑149 (MQ=255) tgtgATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTc < 3:1454449/149‑1 (MQ=255) tgtgATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTc < 1:393458/149‑1 (MQ=255) tgATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAg < 2:1016344/149‑1 (MQ=255) gATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAgg < 2:561766/149‑1 (MQ=255) tATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTaaa < 1:688276/149‑1 (MQ=255) cgcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACaa < 3:248449/149‑1 (MQ=255) cgcTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACaa > 1:1315755/1‑149 (MQ=255) cTGTAATAGATATGTCGAGGGTGAAAACTGTGATACTTGCCTGCCGTTTAGCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCAGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAtt > 2:1031214/1‑149 (MQ=255) tGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAttt > 4:769129/1‑149 (MQ=255) gTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTc < 3:852144/149‑1 (MQ=255) tAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTcc > 3:472327/1‑149 (MQ=255) gATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGttt > 3:64543/1‑149 (MQ=255) atatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGtttt > 4:567812/1‑149 (MQ=255) tatGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGttttt < 1:488137/149‑1 (MQ=255) atGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttc < 2:1308152/149‑1 (MQ=255) ggCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttctt > 1:1131208/1‑149 (MQ=255) gCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttcttc < 1:566190/149‑1 (MQ=255) gatggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct < 1:681067/142‑1 (MQ=255) gggTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCt > 1:583765/1‑149 (MQ=255) gTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTc > 4:749122/1‑149 (MQ=255) tGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTcc > 4:1291022/1‑149 (MQ=255) gAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCt > 4:1455795/1‑149 (MQ=255) aaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg > 1:1190896/1‑149 (MQ=255) aaaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcg > 4:1130217/1‑149 (MQ=255) aaCGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTcga > 3:328629/1‑149 (MQ=255) agaCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTgg < 4:1426783/149‑1 (MQ=255) aCTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGgcg > 1:111280/1‑149 (MQ=255) ctgcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAAt < 1:1217009/108‑1 (MQ=255) ctgcctTTTATCACGGCGTATCCGGCACTTTCGTCAATAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCt < 2:651987/113‑1 (MQ=255) tgcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGT‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCt > 2:571217/1‑149 (MQ=255) tgcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGTAGTGCt > 3:987826/1‑149 (MQ=255) tgcctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCt > 2:243565/1‑149 (MQ=255) cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCAt < 4:66957/122‑1 (MQ=255) cctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgt > 1:680430/1‑149 (MQ=255) ctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTg > 3:764011/1‑110 (MQ=255) ctTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCtgtg < 3:948213/149‑1 (MQ=255) ttttATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTcc > 1:157657/1‑125 (MQ=255) ttttATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTcc < 2:157657/125‑1 (MQ=255) ttttATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGa > 3:1215774/1‑149 (MQ=255) ttttATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGa > 4:605070/1‑149 (MQ=255) ttATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAAt < 4:1364853/149‑1 (MQ=255) tCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTc > 3:86209/1‑149 (MQ=255) cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCa < 3:1212472/149‑1 (MQ=255) cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCa < 3:749122/149‑1 (MQ=255) cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCa < 2:962375/149‑1 (MQ=255) cACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCa < 1:228988/149‑1 (MQ=255) aCGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAg > 4:1318248/1‑149 (MQ=255) gCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCaa > 2:1369483/1‑149 (MQ=255) tATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAAcgc > 3:498996/1‑149 (MQ=255) tATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAAcgc > 1:698896/1‑149 (MQ=255) cGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTGCATCTGCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGttt < 4:939247/149‑1 (MQ=255) ggCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTg > 4:575021/1‑149 (MQ=255) gCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTgc < 3:401047/149‑1 (MQ=255) cTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCACTTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTAGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGca < 4:911691/149‑1 (MQ=255) tttCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcac > 4:350773/1‑149 (MQ=255) cAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCaaa < 4:817326/149‑1 (MQ=255) gTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGttt > 1:1292852/1‑135 (MQ=255) aTCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGc < 2:725210/149‑1 (MQ=255) aTCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGc > 1:725210/1‑149 (MQ=255) tCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTGCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGcc > 2:672948/1‑149 (MQ=255) tCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGcc > 2:1227504/1‑149 (MQ=255) tGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCTGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCta > 2:1291490/1‑149 (MQ=255) tGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCta > 1:1406240/1‑149 (MQ=255) aTTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATaa < 4:86209/149‑1 (MQ=255) tttGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGc > 1:394339/1‑142 (MQ=255) ttGCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGcac < 4:185582/130‑1 (MQ=255) tGCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct < 2:683536/73‑1 (MQ=38) tGCCCGGCTACGT‑GCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct > 1:683536/1‑73 (MQ=38) cccGGC‑ACGTAGCCGTGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCGTCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGc < 1:1117022/149‑1 (MQ=255) ccGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCa > 3:1370720/1‑149 (MQ=255) ccGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCa > 3:1406100/1‑149 (MQ=255) ggC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCaaa > 2:1093976/1‑149 (MQ=255) aCGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct > 3:1048917/1‑65 (MQ=255) aCGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTttct < 4:1048917/65‑1 (MQ=255) aCGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATa > 2:1172246/1‑149 (MQ=255) aCGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATa < 1:64027/149‑1 (MQ=255) cGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATAc < 2:637188/149‑1 (MQ=255) aGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGa < 1:1076703/149‑1 (MQ=255) | CTAAACAGCAGGAAGCTACCCAGCCAGACGATGTGATTCGTATTGCGCTGTAATAGATATGGCGAGGGTGAAAACGGTGAGACTTGCCTGCCTTTTATCACGGCGTATCCGGCACTTTCGTCAGTAATCTGAATTTGCCCGGC‑ACGTAGCCGGGCAATTTTGCAATTAACTCGCCGTTTCAGGCTTAAACACAATTTCCTGTTTTTCTTCATCTTCCTCGACGACTGGCGCAGTGCTGTGAATTTCAGCAACGCGTTTGCGCACACCAAACCAGCCTATAACCAGCAAAATACCGA > NC_000913/1524316‑1524611 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |