Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,623,294 T→A 28.0% V84E (GTG→GAG)  ppk → polyphosphate kinase, component of RNA degradosome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,623,2940TA28.0% 249.6 / 123.4 139V84E (GTG→GAG) ppkpolyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base T (51/49);  new base A (20/19);  total (71/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 140 total aligned reads displayed.

AGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCAGTATCTGC  >  NC_000913/2623146‑2623437
                                                                                                                                                    |                                                                                                                                               
aGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGt                                                                                                                                                 <  1:1188994/149‑1 (MQ=255)
 ggATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTg                                                                                                                                                >  3:1408736/1‑149 (MQ=255)
  gATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGc                                                                                                                                               >  4:1324619/1‑149 (MQ=255)
       gTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGaaa                                                                                                                                          >  1:1346697/1‑149 (MQ=255)
       gTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGaaa                                                                                                                                          <  2:1450107/149‑1 (MQ=255)
            cTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGa                                                                                                                                     >  4:760777/1‑149 (MQ=255)
             tGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGAt                                                                                                                                    >  4:159138/1‑149 (MQ=255)
             tGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGAt                                                                                                                                    >  4:423069/1‑149 (MQ=255)
               gggATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCa                                                                                                                                  <  1:103466/149‑1 (MQ=255)
               gggATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCa                                                                                                                                  >  2:5507/1‑149 (MQ=255)
               gggATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCa                                                                                                                                  <  4:1466539/149‑1 (MQ=255)
               gggATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCa                                                                                                                                  <  1:1242062/149‑1 (MQ=255)
                 gATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAgg                                                                                                                                >  4:64388/1‑149 (MQ=255)
                        tCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGa                                                                                                                         >  3:1268739/1‑149 (MQ=255)
                        tCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGa                                                                                                                         <  4:349000/149‑1 (MQ=255)
                          cAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACg                                                                                                                       >  4:759591/1‑149 (MQ=255)
                          cAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACg                                                                                                                       >  3:1471538/1‑149 (MQ=255)
                          cAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACg                                                                                                                       >  2:918639/1‑149 (MQ=255)
                           aaTAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACgg                                                                                                                      >  4:212706/1‑149 (MQ=255)
                           aaTAACCTTGATGAGTTATATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCACAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACgg                                                                                                                      >  2:1007643/1‑149 (MQ=255)
                                ccTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCctct                                                                                                                 >  2:126625/1‑149 (MQ=255)
                                  ttGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTc                                                                                                                           <  2:243076/137‑1 (MQ=255)
                                  ttGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTAc                                                                                                               >  4:848195/1‑149 (MQ=255)
                                      tgaGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACg                                                                                                           <  3:277207/149‑1 (MQ=255)
                                       gaGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGa                                                                                                          <  1:1347451/149‑1 (MQ=255)
                                        aGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAg                                                                                                         <  1:1264433/149‑1 (MQ=255)
                                            cTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTAt                                                                                                     <  3:1149569/149‑1 (MQ=255)
                                            cTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTAt                                                                                                     >  2:882240/1‑149 (MQ=255)
                                             tataAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTAtt                                                                                                    >  3:597693/1‑149 (MQ=255)
                                             tataAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTAtt                                                                                                    <  1:378179/149‑1 (MQ=255)
                                               taAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGc                                                                                                  >  3:1152650/1‑149 (MQ=255)
                                               taAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGc                                                                                                  >  4:456216/1‑149 (MQ=255)
                                                 aaGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTg                                                                                                <  3:64388/149‑1 (MQ=255)
                                                  aGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTgg                                                                                               <  2:1011209/149‑1 (MQ=255)
                                                   gTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGga                                                                                              <  4:1462729/149‑1 (MQ=255)
                                                    tCCGCTTCGCTGAACTGAAGCGACGCCTCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCATCTACAACGAGCTATTGCTGgag                                                                                             >  1:1405547/1‑149 (MQ=255)
                                                    tCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGATGAACAACGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCGGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGgag                                                                                             >  4:441496/1‑149 (MQ=255)
                                                    tCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGaaa                                                                                                                                          >  4:225131/1‑104 (MQ=255)
                                                    tCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGgag                                                                                             >  1:1438203/1‑149 (MQ=255)
                                                     ccGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGgaga                                                                                            <  3:1437196/149‑1 (MQ=255)
                                                        cTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATgg                                                                                         >  3:317115/1‑149 (MQ=255)
                                                        cTTCGCTGAACTGAAGCGAAGCATCATTATTAGCGAAGAACAACGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGCCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATgg                                                                                         >  2:435749/1‑149 (MQ=255)
                                                         ttCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGgc                                                                                        <  1:777336/149‑1 (MQ=255)
                                                         ttCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGgc                                                                                        <  1:804013/149‑1 (MQ=255)
                                                              tgaactgaaGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCa                                                                                   >  1:804619/1‑149 (MQ=255)
                                                                aactgaaGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAAc                                                                                 <  1:50789/149‑1 (MQ=255)
                                                                aactgaaGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAAc                                                                                 <  3:850599/149‑1 (MQ=255)
                                                                   tgaaGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGga                                                                                              >  2:740692/1‑133 (MQ=255)
                                                                   tgaaGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGga                                                                                              <  1:740692/133‑1 (MQ=255)
                                                                    gaaGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGa                                                                             <  3:581240/149‑1 (MQ=255)
                                                                       gCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCt                                                                          >  3:1081272/1‑149 (MQ=255)
                                                                         gACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTc                                                                        <  1:1484669/149‑1 (MQ=255)
                                                                         gACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTc                                                                        <  2:260483/149‑1 (MQ=255)
                                                                         gACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTc                                                                        <  3:692581/149‑1 (MQ=255)
                                                                            gCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATTTTCCTg                                                                     >  3:46998/1‑149 (MQ=255)
                                                                             catcatTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGa                                                                    <  3:423069/149‑1 (MQ=255)
                                                                             catcatTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGa                                                                    <  1:1047491/149‑1 (MQ=255)
                                                                             catcatTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGa                                                                    <  2:526794/149‑1 (MQ=255)
                                                                              atcatTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGAt                                                                   <  2:1160741/149‑1 (MQ=255)
                                                                                 attattAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTaa                                                                <  3:355403/149‑1 (MQ=255)
                                                                                  ttattaGCGAAGAACAAGGCTCCATCACTCATGCCCGACATATACTGGGCAAAATTCAGTCCCAGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGATATTGCGCGCAACCCGATCTTCCTGATTAAt                                                               <  2:298810/149‑1 (MQ=255)
                                                                                      taGCGAAGCACGAGGGTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATTTTCCTGATTAATGAAc                                                           <  2:1261610/149‑1 (MQ=255)
                                                                                          gaagaaCAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCa                                                       <  3:243258/149‑1 (MQ=255)
                                                                                          gaagaaCAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCa                                                       <  3:848195/149‑1 (MQ=255)
                                                                                           aagaaCAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAg                                                      >  4:631160/1‑149 (MQ=255)
                                                                                           aagaaCAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAg                                                      >  3:1267479/1‑149 (MQ=255)
                                                                                               aCAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACAAGATCTTCCTGATTAATCAACGCCAGctat                                                  >  1:453444/1‑147 (MQ=255)
                                                                                                 aaGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTcc                                                >  4:84331/1‑149 (MQ=255)
                                                                                                        cAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGtcaatc                                         >  1:621384/1‑149 (MQ=255)
                                                                                                         aaCTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGtcaatca                                        >  2:9031/1‑149 (MQ=255)
                                                                                                         aaCTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGtcaatca                                        >  3:1214215/1‑149 (MQ=255)
                                                                                                            tctcATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATcaaca                                     >  1:272339/1‑149 (MQ=255)
                                                                                                            tctcATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATcaaca                                     >  4:1294694/1‑149 (MQ=255)
                                                                                                              tcATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACaaa                                   >  3:514887/1‑149 (MQ=255)
                                                                                                                aTTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAAc                                 >  4:874097/1‑149 (MQ=255)
                                                                                                                aTTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCAATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAAc                                 >  4:1392677/1‑149 (MQ=255)
                                                                                                                    ccGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTc                                                                        >  2:793192/1‑106 (MQ=255)
                                                                                                                    ccGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTc                                                                        <  1:793192/106‑1 (MQ=255)
                                                                                                                     cGCCATTTACTGGGCAAAATTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCt                            >  2:391480/1‑149 (MQ=255)
                                                                                                                      gCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTTgaga                                                                                            >  1:441225/1‑84 (MQ=255)
                                                                                                                      gCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTg                           >  2:1185656/1‑149 (MQ=255)
                                                                                                                        cATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGaa                                                            >  3:492887/1‑114 (MQ=255)
                                                                                                                        cATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGaa                                                            <  4:492887/114‑1 (MQ=255)
                                                                                                                          tttACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTc                       >  2:1058963/1‑149 (MQ=255)
                                                                                                                                 ggCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCa                                                       <  4:1120307/110‑1 (MQ=255)
                                                                                                                                 ggCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCa                                                       >  3:1120307/1‑110 (MQ=255)
                                                                                                                                 ggCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTAttt                <  1:1058963/149‑1 (MQ=255)
                                                                                                                                    aaaaTTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTaa             <  4:596806/149‑1 (MQ=255)
                                                                                                                                     aaaTTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAg            <  3:84331/149‑1 (MQ=255)
                                                                                                                                      aaTTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAg                                                                              <  3:475548/82‑1 (MQ=255)
                                                                                                                                      aaTTCAGTCACGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAg                                                                              >  4:475548/1‑82 (MQ=255)
                                                                                                                                       aTTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCa          <  4:1408736/149‑1 (MQ=255)
                                                                                                                                       aTTCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATTTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCa          <  4:46998/149‑1 (MQ=255)
                                                                                                                                        ttCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGtcaatc                                         <  1:113344/117‑1 (MQ=255)
                                                                                                                                         tCAGTCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCAGt        <  2:1050768/149‑1 (MQ=255)
                                                                                                                                             tCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCAGTATCt    <  1:126625/149‑1 (MQ=255)
                                                                                                                                             tCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCa          >  2:558175/1‑143 (MQ=255)
                                                                                                                                             tCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCa          <  1:558175/143‑1 (MQ=255)
                                                                                                                                             tCCCGGGAGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCAGTATCt    <  1:970149/149‑1 (MQ=255)
                                                                                                                                               ccGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCAGTATCTGc  <  3:617418/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
AGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGCTGGAGATGGCGCGCAACCAGATCTTCCTGATTAATGAACGCCAGCTCTCCGTCAATCAACAAAACTGGCTGCGTCATTATTTTAAGCAGTATCTGC  >  NC_000913/2623146‑2623437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: