Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 431,107 A→T 5.7% intergenic (+155/+22) yajD → / ← tsx HNH nuclease family protein/nucleoside channel, receptor of phage T6 and colicin K

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913431,1070AT 5.7% 547.6 / 17.0 158intergenic (+155/+22)yajD/tsxHNH nuclease family protein/nucleoside channel, receptor of phage T6 and colicin K
Reads supporting (aligned to +/- strand):  ref base A (80/69);  new base T (6/3);  total (86/72)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 160 total aligned reads displayed.

CGTAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTAC  >  NC_000913/430959‑431254
                                                                                                                                                    |                                                                                                                                                   
cGTAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGaa                                                                                                                                                     <  2:232580/149‑1 (MQ=255)
    aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATaaa                                                                                                                                                 >  1:404664/1‑149 (MQ=255)
    aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATaaa                                                                                                                                                 >  2:18914/1‑149 (MQ=255)
        tGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATccc                                                                                                                                             <  1:294626/149‑1 (MQ=255)
        tGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATccc                                                                                                                                             <  2:1271822/149‑1 (MQ=255)
         gCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCg                                                                                                                                            <  4:1182411/149‑1 (MQ=255)
         gCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTCACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCg                                                                                                                                            <  4:420601/149‑1 (MQ=255)
              aTGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCAtt                                                                                                                                       >  1:1344316/1‑149 (MQ=255)
                 cgcTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTc                                                                                                                                    <  4:83523/149‑1 (MQ=255)
                 cgcTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTc                                                                                                                                    <  1:422915/149‑1 (MQ=255)
                   cTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCAt                                                                                                                                  >  4:714849/1‑149 (MQ=255)
                    tACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATa                                                                                                                                 >  2:1494363/1‑149 (MQ=255)
                      cGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAAt                                                                                                                               <  2:298825/149‑1 (MQ=255)
                         ttATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAg                                                                                                                            >  3:747861/1‑149 (MQ=255)
                           aTCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGaa                                                                                                                          <  2:240235/149‑1 (MQ=255)
                           aTCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGaa                                                                                                                          >  4:32213/1‑149 (MQ=255)
                           aTCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAAGGCCGGGAATAAATCCCGGCATTTTCATAATCAGaa                                                                                                                          <  4:658872/149‑1 (MQ=255)
                              aGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGtt                                                                                                                       >  4:1204160/1‑149 (MQ=255)
                                  cTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTaa                                                                                                                   >  1:1050180/1‑149 (MQ=255)
                                  cTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCACGGCATTTTCATAATCAGAAGTCGTaa                                                                                                                   >  4:1347260/1‑149 (MQ=255)
                                   tACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAAc                                                                                                                  >  2:433699/1‑149 (MQ=255)
                                   tACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAAc                                                                                                                  >  2:1184072/1‑149 (MQ=255)
                                   tACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAAc                                                                                                                  <  1:1184072/149‑1 (MQ=255)
                                      gTTATTTCAGCAATATATAGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACcta                                                                                                               >  2:525954/1‑149 (MQ=255)
                                         aTTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACctacta                                                                                                            <  3:1373890/149‑1 (MQ=255)
                                              aGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAgg                                                                                                       >  2:625643/1‑149 (MQ=255)
                                              aGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAgg                                                                                                       >  4:779827/1‑149 (MQ=255)
                                               gCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGt                                                                                                      >  2:581662/1‑149 (MQ=255)
                                                cAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTa                                                                                                     <  1:581662/149‑1 (MQ=255)
                                                 aaTATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTaa                                                                                                    >  4:1048429/1‑149 (MQ=255)
                                                 aaTATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTaa                                                                                                    <  3:779827/149‑1 (MQ=255)
                                                      atTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAaccacc                                                                                               >  1:50523/1‑149 (MQ=255)
                                                       ttGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATACTCAGAAGTTGTAACCTACTACCAGGTAaccacac                                                                                              >  3:1209910/1‑147 (MQ=255)
                                                       ttGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCAccc                                                                                              >  2:227839/1‑149 (MQ=255)
                                                         gAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCa                                                                                            <  2:307152/149‑1 (MQ=255)
                                                          aaTTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAg                                                                                           >  4:143454/1‑149 (MQ=255)
                                                           aTTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGc                                                                                          <  3:570956/149‑1 (MQ=255)
                                                           aTTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATACGGCATGAACAAAGCACACGTTGTTAAAAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGc                                                                                          >  4:1369443/1‑149 (MQ=255)
                                                            tttACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGcc                                                                                         >  4:593609/1‑149 (MQ=255)
                                                              tACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCgg                                                                                       <  2:466340/149‑1 (MQ=255)
                                                                cGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTa                                                                                     <  1:1488139/149‑1 (MQ=255)
                                                                cGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTa                                                                                     <  1:1457454/149‑1 (MQ=255)
                                                                  tGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTaga                                                                                   <  1:227839/149‑1 (MQ=255)
                                                                  tGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTaga                                                                                   <  3:703082/149‑1 (MQ=255)
                                                                   gCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTagag                                                                                  <  4:18663/149‑1 (MQ=255)
                                                                     ttttGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCg                                                                                >  4:1035866/1‑149 (MQ=255)
                                                                       ttgttgGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGaa                                                                              <  4:836710/149‑1 (MQ=255)
                                                                        tgttgGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAAc                                                                             <  2:50523/149‑1 (MQ=255)
                                                                           tgGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGtt                                                                          <  1:18914/149‑1 (MQ=255)
                                                                              ccGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGaa                                                                       <  4:1298201/149‑1 (MQ=255)
                                                                                 gACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgtt                                                                    >  1:894541/1‑149 (MQ=255)
                                                                                  aCAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttg                                                                   >  3:425809/1‑149 (MQ=255)
                                                                                    aaaGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGGATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgcc                                                                 >  4:969981/1‑149 (MQ=255)
                                                                                    aaaGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgcc                                                                 >  2:856130/1‑149 (MQ=255)
                                                                                    aaaGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgcc                                                                 <  2:1050180/149‑1 (MQ=255)
                                                                                     aaGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccg                                                                >  1:121902/1‑149 (MQ=255)
                                                                                         gTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgttgc                                                            <  2:1345634/149‑1 (MQ=255)
                                                                                             tcgccgcATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGTATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCATCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGaa                                                        <  1:521446/148‑1 (MQ=255)
                                                                                              cgccgcATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAag                                                       <  1:1052035/149‑1 (MQ=255)
                                                                                               gccgcATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagt                                                      <  3:1075577/149‑1 (MQ=255)
                                                                                               gacgcATCCGGCATGAACAAAGCACACGTTGTTAAAAATCAGAAATGCCGGGAATAAATCCCGGAATTTTCATCATCAGAAGTTGTACCCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagt                                                      >  2:1377135/3‑149 (MQ=255)
                                                                                                ccgcATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagtt                                                     >  1:462577/1‑149 (MQ=255)
                                                                                                   cATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttcag                                                  <  3:258713/149‑1 (MQ=255)
                                                                                                    aTCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttcagt                                                 >  3:1406783/1‑149 (MQ=255)
                                                                                                       cGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCt                                              <  3:896494/149‑1 (MQ=255)
                                                                                                            tGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAtc                                         <  4:537413/149‑1 (MQ=255)
                                                                                                              aaCAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAtcgt                                       >  2:875624/1‑149 (MQ=255)
                                                                                                               aCAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATACCGGCATTTACATAAACAGAAGTTGTAACCTACTACCAGGTAACTACCGCAGTCGGTAGAGCGAACGTTGAAGTTGCCGTAGCCGAAGAGCAGTTCTGCAtcgtc                                      >  4:1004676/1‑149 (MQ=255)
                                                                                                                   aGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGaa                                                                       <  4:448718/112‑1 (MQ=255)
                                                                                                                   aGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTc                                  <  1:336240/149‑1 (MQ=255)
                                                                                                                       cacGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACt                              <  1:646436/149‑1 (MQ=255)
                                                                                                                        acGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTg                             >  3:827049/1‑149 (MQ=255)
                                                                                                                        acGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAATTCTGCATCGTCGTTCCACTg                             >  4:1398974/1‑149 (MQ=255)
                                                                                                                         cGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGa                            <  1:871726/149‑1 (MQ=255)
                                                                                                                          gttgttAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGac                           >  2:451612/1‑149 (MQ=255)
                                                                                                                             gttAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGaccac                        >  3:896982/1‑149 (MQ=255)
                                                                                                                               tAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACcg                      <  3:115332/149‑1 (MQ=255)
                                                                                                                                aaCAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACcgt                     >  2:544590/1‑149 (MQ=255)
                                                                                                                                     tCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGc                <  3:143454/149‑1 (MQ=255)
                                                                                                                                     tCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGc                >  3:251183/1‑149 (MQ=255)
                                                                                                                                     tCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGc                >  3:1216487/1‑149 (MQ=255)
                                                                                                                                      tgaaaaTGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGcc               >  4:269208/4‑149 (MQ=255)
                                                                                                                                       aGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCa              <  4:773246/149‑1 (MQ=255)
                                                                                                                                       aGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCa              <  4:609261/149‑1 (MQ=255)
                                                                                                                                        gAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGac                           <  3:1482350/135‑1 (MQ=255)
                                                                                                                                        gAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGac                           >  4:1482350/1‑135 (MQ=255)
                                                                                                                                        gAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAg             >  2:884727/1‑149 (MQ=255)
                                                                                                                                         aaaTGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGt            >  3:1256162/1‑149 (MQ=255)
                                                                                                                                           aTGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTaa          <  4:441324/149‑1 (MQ=255)
                                                                                                                                            tGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttca                                                   >  3:707071/1‑107 (MQ=255)
                                                                                                                                            tGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAAc         <  4:22597/149‑1 (MQ=255)
                                                                                                                                            tGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAAc         <  3:1408822/149‑1 (MQ=255)
                                                                                                                                             gCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAg             >  1:1293790/1‑144 (MQ=255)
                                                                                                                                              ccGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCTGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGa       <  4:1364831/149‑1 (MQ=255)
                                                                                                                                               cGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAg      >  2:1143682/1‑149 (MQ=255)
                                                                                                                                               cGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAg      >  3:37504/1‑149 (MQ=255)
                                                                                                                                                gggAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTaa          >  4:499272/1‑144 (MQ=255)
                                                                                                                                                gggAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTaa          <  3:499272/144‑1 (MQ=255)
                                                                                                                                                 ggAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCt    >  3:585037/1‑149 (MQ=255)
                                                                                                                                                   aaTAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTAc  <  4:1161713/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTAC  >  NC_000913/430959‑431254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: