Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,470,108 A→C 5.3% intergenic (‑255/+37) chiA ← / ← tufA periplasmic endochitinase/translation elongation factor EF‑Tu 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,470,1080AC 5.3% 561.0 / 16.5 163intergenic (‑255/+37)chiA/tufAperiplasmic endochitinase/translation elongation factor EF‑Tu 1
Reads supporting (aligned to +/- strand):  ref base A (75/79);  new base C (5/4);  total (80/83)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

GAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCATT  >  NC_000913/3469961‑3470256
                                                                                                                                                    |                                                                                                                                                    
gAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGaa                                                                                                                                                     >  1:1210557/1‑149 (MQ=255)
     aaGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGcc                                                                                                                                                <  4:1384867/149‑1 (MQ=255)
        acTGACAAAATGAACAATTTAAAAAAAAAAAACATTAAATTTAAAATGTTAAAATGAAATGATAATTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtt                                                                                                                                             <  2:974922/148‑1 (MQ=255)
          tGACAAATGAAGCATTTTAAAAACAGAAACATTTATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGTGCGCCGAAGCGCCCtttt                                                                                                                                           >  1:514178/1‑149 (MQ=255)
          tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCtttt                                                                                                                                           <  1:1020041/149‑1 (MQ=255)
          tGACAAATGAAACATTTTAACACAAGAAACATTCATCTTTACAATGTTAAATTGAATTGATATTTAAAACAAAAATAATTTATTTGTCTCGACAGTCAGAATAAGTTCTGCCGACAACAAATGCAAAATGGCCGCCGAAGCGaccaaat                                                                                                                                           >  4:434827/1‑145 (MQ=255)
           gACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttc                                                                                                                                          >  4:977562/1‑149 (MQ=255)
            aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttca                                                                                                                                         >  1:606010/1‑149 (MQ=255)
               aaaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaat                                                                                                                                       >  2:248281/1‑149 (MQ=255)
                aaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaatt                                                                                                                                      <  4:1053301/149‑1 (MQ=255)
                  tGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaattca                                                                                                                                    >  1:1091021/1‑149 (MQ=255)
                     aGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCaaaa                                                                                                                                 >  1:1338114/1‑149 (MQ=255)
                     aGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCaaaa                                                                                                                                 <  2:1273273/149‑1 (MQ=255)
                       cATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACt                                                                                                                               >  4:1432389/1‑149 (MQ=255)
                         ttttAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTaa                                                                                                                             >  4:791732/1‑149 (MQ=255)
                              aaaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                        <  4:1289544/149‑1 (MQ=255)
                              aaaaCAGAAACAATCATATTTAAAATGATAAAATGAATTGATAATTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAc                                                                                                                        <  1:572912/149‑1 (MQ=255)
                               aaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACg                                                                                                                       >  1:10624/1‑149 (MQ=255)
                                aaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgt                                                                                                                      <  2:766839/149‑1 (MQ=255)
                                aaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgt                                                                                                                      <  1:921978/149‑1 (MQ=255)
                                aaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgt                                                                                                                      >  4:352670/1‑149 (MQ=255)
                                   aGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTa                                                                                                                   >  4:545678/1‑149 (MQ=255)
                                       aCATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTa                                                                                                               <  2:10624/149‑1 (MQ=255)
                                        cATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAg                                                                                                              <  4:1386507/149‑1 (MQ=255)
                                          ttCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGcc                                                                                                            <  3:196203/149‑1 (MQ=255)
                                           tCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGccc                                                                                                           >  1:928030/1‑149 (MQ=255)
                                             atatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAg                                                                                                         >  3:125768/1‑149 (MQ=255)
                                                tttAAAATGTTAAATTGAATTGATATTTTAATTATGAATAATTTATTCGTTCTGACAGTACGAATAAAATATGCCGTCTACAAATTCAAAATGGGCGCCGCAGCGCCCTTTTCAATTCAAAACTAATTACCGTGAAATTAGCCCAGAAc                                                                                                      >  2:706487/1‑149 (MQ=255)
                                                tttAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAAc                                                                                                      >  4:821729/1‑149 (MQ=255)
                                                    aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTa                                                                                                  <  2:1107875/149‑1 (MQ=255)
                                                    aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTa                                                                                                  <  3:352670/149‑1 (MQ=255)
                                                    aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTa                                                                                                  <  1:260929/149‑1 (MQ=255)
                                                    aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTa                                                                                                  <  3:1108286/149‑1 (MQ=255)
                                                      aTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGc                                                                                                <  3:909575/149‑1 (MQ=255)
                                                         ttAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTaa                                                                                                                             >  3:806495/1‑117 (MQ=255)
                                                         ttAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTaa                                                                                                                             <  4:806495/117‑1 (MQ=255)
                                                         ttAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGcaac                                                                                             >  2:1502618/1‑149 (MQ=255)
                                                            aattgaattgATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACtt                                                                                                    >  4:1146983/1‑139 (MQ=255)
                                                            aattgaattgATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACtt                                                                                                    <  3:1146983/139‑1 (MQ=255)
                                                                 aattgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTaa                                                                                                                  <  3:260547/120‑1 (MQ=255)
                                                                  attgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCcgc                                                                                    <  2:1218609/149‑1 (MQ=255)
                                                                  attgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCcgc                                                                                    <  3:1311003/149‑1 (MQ=255)
                                                                  attgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCcgc                                                                                    >  1:1158414/1‑149 (MQ=255)
                                                                            tAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTa                                                                          <  1:1053188/149‑1 (MQ=255)
                                                                              aaTATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACg                                                                        >  3:77947/1‑149 (MQ=255)
                                                                                tatGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgc                                                                      >  1:839836/1‑149 (MQ=255)
                                                                                  tGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccg                                                                    >  2:530404/1‑149 (MQ=255)
                                                                                   gAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccgc                                                                   >  1:112228/1‑149 (MQ=255)
                                                                                   gAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccgc                                                                   <  4:342750/149‑1 (MQ=255)
                                                                                    aataatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccgcc                                                                  >  2:32722/1‑149 (MQ=255)
                                                                                     ataatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCt                                                                 >  3:157297/1‑149 (MQ=255)
                                                                                      taatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAAt                                                                                                                 >  4:497740/1‑100 (MQ=255)
                                                                                      taatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAAt                                                                                                                 <  3:497740/100‑1 (MQ=255)
                                                                                         tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCaacaac                                                                                          <  3:544844/120‑1 (MQ=255)
                                                                                         tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCAc                                                             >  3:777462/1‑149 (MQ=255)
                                                                                         tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCAc                                                             <  2:1324570/149‑1 (MQ=255)
                                                                                         tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCAc                                                             >  4:274879/1‑149 (MQ=255)
                                                                                          ttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAGCGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACg                                                            <  4:1240756/149‑1 (MQ=255)
                                                                                             ttCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAt                                                         >  4:189605/1‑149 (MQ=255)
                                                                                              tCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAtt                                                        <  3:1204722/149‑1 (MQ=255)
                                                                                                gTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGc                                                      >  3:1389412/1‑149 (MQ=255)
                                                                                                        aGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTCCACGGATTGCGAAACGCa                                              >  2:1092989/1‑149 (MQ=255)
                                                                                                          tACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGa                                            <  2:1338114/149‑1 (MQ=255)
                                                                                                          tACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGa                                            <  2:912098/149‑1 (MQ=255)
                                                                                                               aTAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcgtc                                       <  2:112228/149‑1 (MQ=255)
                                                                                                                 aaGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcgtcgt                                     <  2:104098/149‑1 (MQ=255)
                                                                                                                 aaGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATCGCGAAACGCAGACcgtcgt                                     >  4:1011889/1‑149 (MQ=255)
                                                                                                                   gATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAAt                                                                                                                            <  2:551756/60‑1 (MQ=255)
                                                                                                                   gATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTcc                                   >  1:1427034/1‑149 (MQ=255)
                                                                                                                   gATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTcc                                   >  2:203003/1‑149 (MQ=255)
                                                                                                                     tatGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGaa                                                                                                       <  1:674431/79‑1 (MQ=255)
                                                                                                                     tatGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCAt                                 <  1:18023/149‑1 (MQ=255)
                                                                                                                      atGCCGTCAACAGATGCAAAAAGGGCGCCGAAGCGCCCGTTTGAAATCAAAACTAATTGACGTGGAATTAGCCCAGAACTTTAGCATAAGCGCACGCTCCAACGGTACGGCCGCGTACACGGATTGCTAATAGCAGGCCGTcgtccgtc                                <  4:118589/149‑1 (MQ=255)
                                                                                                                      atGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATc                                <  3:859816/149‑1 (MQ=255)
                                                                                                                       tGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATcg                               <  4:1059519/149‑1 (MQ=255)
                                                                                                                           gTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGAt                           <  3:624573/149‑1 (MQ=255)
                                                                                                                           gTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGAt                           >  4:1464128/1‑149 (MQ=255)
                                                                                                                               acaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCggg                       >  2:1065258/1‑149 (MQ=255)
                                                                                                                                caaAAGCAAAAAGGTCGCCGAAGCGCCCTTTTCAATTCAAAACGGATTAACGTGTAAGTAGCCCAGAACTTTAGCAGCAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGt                      <  1:812316/149‑1 (MQ=21)
                                                                                                                                 aaaTGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTg                     <  4:522368/149‑1 (MQ=255)
                                                                                                                                 aaaTGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTg                     <  1:206995/149‑1 (MQ=255)
                                                                                                                                    tGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGAt                  >  1:542605/1‑149 (MQ=255)
                                                                                                                                    tGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGAt                  <  4:1437179/149‑1 (MQ=255)
                                                                                                                                     gCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATc                 <  4:593752/149‑1 (MQ=255)
                                                                                                                                     gCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATc                 <  1:1157817/149‑1 (MQ=255)
                                                                                                                                       aaaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAg               >  3:620008/1‑149 (MQ=255)
                                                                                                                                        aaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCCGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTg                     >  1:47795/1‑142 (MQ=21)
                                                                                                                                        aaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCCGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTg                     <  2:47795/142‑1 (MQ=21)
                                                                                                                                        aaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGt                      <  3:998632/141‑1 (MQ=255)
                                                                                                                                        aaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAgg              >  3:367881/1‑149 (MQ=255)
                                                                                                                                        aaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAgg              <  2:66641/149‑1 (MQ=255)
                                                                                                                                         aaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAggg             <  1:1502618/149‑1 (MQ=255)
                                                                                                                                         aaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAggg             <  3:470796/149‑1 (MQ=255)
                                                                                                                                          aaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGt            >  4:574087/1‑149 (MQ=255)
                                                                                                                                           aGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTa           >  3:1050838/1‑149 (MQ=255)
                                                                                                                                           aGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTa           <  2:445820/149‑1 (MQ=255)
                                                                                                                                            gggCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTaa          <  2:185555/149‑1 (MQ=255)
                                                                                                                                               cgcCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCgg                        <  2:165962/132‑1 (MQ=255)
                                                                                                                                                  cGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATc                 <  4:24696/136‑1 (MQ=255)
                                                                                                                                                    aaGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAATGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCAtt  <  3:1011889/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCATT  >  NC_000913/3469961‑3470256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: