Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,845,746 G→A 5.2% intergenic (+16/+30) adeD → / ← uhpT cryptic adenine deaminase/hexose phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,845,7460GA 5.2% 441.5 / 9.3 134intergenic (+16/+30)adeD/uhpTcryptic adenine deaminase/hexose phosphate transporter
Reads supporting (aligned to +/- strand):  ref base G (72/55);  new base A (4/3);  total (76/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01

Only 100 of 135 total aligned reads displayed.

TCCGTTACCCGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAG  >  NC_000913/3845598‑3845883
                                                                                                                                                    |                                                                                                                                         
tCCGTTACCCGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTCATGGCGAGATGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCg                                                                                                                                           >  4:1019219/1‑149 (MQ=255)
  cGTTACCCGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTc                                                                                                                                         <  3:250067/149‑1 (MQ=255)
   gTTACCCGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCa                                                                                                                                        >  1:1202712/1‑149 (MQ=255)
        ccGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCg                                                                                                                                   >  4:808353/1‑149 (MQ=255)
        ccGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCg                                                                                                                                   >  2:842914/1‑149 (MQ=255)
         cGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCgg                                                                                                                                  <  3:324645/149‑1 (MQ=255)
          gATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCggg                                                                                                                                 <  2:760333/149‑1 (MQ=255)
               gCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCaaaa                                                                                                                            <  2:605512/149‑1 (MQ=255)
               gCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCaaaa                                                                                                                            <  2:134357/149‑1 (MQ=255)
                ccGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAg                                                                                                                           >  4:1194722/1‑149 (MQ=255)
                ccGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCATGACGCCGGGcttttt                                                                                                                           <  3:842073/149‑6 (MQ=255)
                  gTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGTCGTCATGCCGGGCAAAAGTc                                                                                                                         >  2:188887/1‑149 (MQ=255)
                    ttATTCAGATGGCGTTTCTTTCTCCGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCAc                                                                                                                       <  1:1050503/149‑1 (MQ=255)
                      aTTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCa                                                                                                                     <  2:382466/149‑1 (MQ=255)
                         cAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGtt                                                                                                                  >  2:1469254/1‑149 (MQ=255)
                          aGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTa                                                                                                                 >  1:1121191/1‑149 (MQ=255)
                          aGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTa                                                                                                                 >  1:495806/1‑149 (MQ=255)
                               gCGTTTCTTTCTCTGCCAGTGATCCCCGAGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGttt                                                                                                            >  3:101057/1‑149 (MQ=255)
                                 gTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTAt                                                                                                          >  2:1237688/1‑149 (MQ=255)
                                       ttctCTGCCAGAGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACt                                                                                                    >  3:867315/1‑149 (MQ=255)
                                        tctcTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTg                                                                                                   >  4:117854/1‑149 (MQ=255)
                                          tctGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTc                                                                                                 >  3:1198175/1‑149 (MQ=255)
                                             gCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAAc                                                                                              >  1:560395/1‑149 (MQ=255)
                                              ccAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAAct                                                                                             >  3:30755/1‑149 (MQ=255)
                                                 gTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAActgct                                                                                          >  1:130386/1‑149 (MQ=255)
                                                    aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGGCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGaa                                                                                       <  1:1364018/149‑1 (MQ=255)
                                                    aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCa                                                                                                                        >  4:479712/1‑116 (MQ=255)
                                                    aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGaa                                                                                       <  1:658841/149‑1 (MQ=255)
                                                    aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGaa                                                                                       >  2:67515/1‑149 (MQ=255)
                                                    aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGGCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGaa                                                                                       <  2:829537/149‑1 (MQ=255)
                                                       cccGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAAttt                                                                                    >  1:623207/1‑149 (MQ=255)
                                                        ccGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAAtttt                                                                                   <  3:879059/149‑1 (MQ=255)
                                                         cgcgCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAAttttt                                                                                  <  3:808353/149‑1 (MQ=255)
                                                         cgcgCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAAttttt                                                                                  <  2:623207/149‑1 (MQ=255)
                                                                   cTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCgcggc                                                                        >  3:1485183/1‑149 (MQ=255)
                                                                      aCCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGa                                                                     <  2:101292/149‑1 (MQ=255)
                                                                           tCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTg                                                                <  1:7212/149‑1 (MQ=255)
                                                                              ggggCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGt                                                             <  4:1150692/149‑1 (MQ=255)
                                                                              ggggCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGt                                                             <  3:960639/149‑1 (MQ=255)
                                                                                ggCTATTTGATGGCGAGAAGTTTGCCTTCACTTCGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGgtc                                                           <  2:556728/149‑1 (MQ=255)
                                                                                ggCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttc                                                           >  4:591448/1‑149 (MQ=255)
                                                                                ggCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttc                                                           >  3:1302415/1‑149 (MQ=255)
                                                                                  cTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttctt                                                         <  1:1419789/149‑1 (MQ=255)
                                                                                   tATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttcttc                                                        >  2:1416731/1‑149 (MQ=255)
                                                                                   tATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttcttc                                                        >  4:1436151/1‑149 (MQ=255)
                                                                                     tttGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCa                                                      <  4:1375829/149‑1 (MQ=255)
                                                                                         aTGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTAc                                                  >  1:1110626/1‑149 (MQ=255)
                                                                                         aTGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTAc                                                  <  3:710653/149‑1 (MQ=255)
                                                                                           ggCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTcgc                                                                           >  1:333675/1‑122 (MQ=255)
                                                                                           ggCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTcgc                                                                           <  2:333675/122‑1 (MQ=255)
                                                                                              gcgaAGTTTGCCTTCACTACGCTGGAAGTCCCGGAATGATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCa                                             >  3:595314/3‑149 (MQ=255)
                                                                                                   gTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATc                                        <  2:871011/149‑1 (MQ=255)
                                                                                                    tttGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCg                                       >  2:1196772/1‑149 (MQ=255)
                                                                                                    attGCCATCACTACGCTCGAGGACACGAAAAAATAAAAAAAGCCTGGCGTCATGCCGGGCAAACGTCACCAGTTACGTTGATGCCACAGACCACTGCTGAATTTTTTTCTCGCGGCGGATATTGCGTTCTTCCATTACCGCCACTATCg                                       >  4:172143/2‑149 (MQ=255)
                                                                                                       gCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAGGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCa                                    <  2:173004/149‑1 (MQ=255)
                                                                                                        ccTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCGAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCAt                                   >  4:127335/1‑149 (MQ=255)
                                                                                                         cTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATc                                  >  3:14403/1‑149 (MQ=255)
                                                                                                          ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                 >  3:625682/1‑149 (MQ=255)
                                                                                                          ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                 >  1:1048300/1‑149 (MQ=255)
                                                                                                          ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                 >  1:1392024/1‑149 (MQ=255)
                                                                                                          ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                 <  1:929014/149‑1 (MQ=255)
                                                                                                          ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                 <  2:560395/149‑1 (MQ=255)
                                                                                                          ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                 <  2:517455/149‑1 (MQ=255)
                                                                                                            cACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAgg                               <  2:1033198/149‑1 (MQ=255)
                                                                                                             aCTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGc                              >  4:696273/1‑149 (MQ=255)
                                                                                                              cTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCa                             <  2:1048300/149‑1 (MQ=255)
                                                                                                               tACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttc                                                           <  1:736899/118‑1 (MQ=255)
                                                                                                               tACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttc                                                           >  2:736899/1‑118 (MQ=255)
                                                                                                               tACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAg                            >  1:298884/1‑149 (MQ=255)
                                                                                                               tACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAg                            >  1:773665/1‑149 (MQ=255)
                                                                                                               tACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAg                            >  2:1189945/1‑149 (MQ=255)
                                                                                                                  gCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATa                         >  4:855375/1‑149 (MQ=255)
                                                                                                                    tGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACa                       >  2:1399986/1‑149 (MQ=255)
                                                                                                                    tGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACa                       >  3:512242/1‑149 (MQ=255)
                                                                                                                     ggAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACaa                      <  2:1121191/149‑1 (MQ=255)
                                                                                                                     ggAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACaa                      <  4:1002430/149‑1 (MQ=255)
                                                                                                                     ggAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTAAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACaa                      <  3:890030/149‑1 (MQ=255)
                                                                                                                      gAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAAc                     <  2:803536/149‑1 (MQ=255)
                                                                                                                            aCGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATcg               >  1:1012238/1‑149 (MQ=255)
                                                                                                                            aCGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATcg               >  3:122091/1‑149 (MQ=255)
                                                                                                                            aCGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGCCAGATACAACCAATcg               >  1:149327/1‑149 (MQ=255)
                                                                                                                              ggAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATcgcg             >  1:426121/1‑149 (MQ=255)
                                                                                                                                aataatAAAAAAAGCCCGGCGTCATTCCGGGCAAAAGTCACCAGTTACTTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCtcggc           <  2:28629/149‑1 (MQ=255)
                                                                                                                                aataatAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCgcggc           <  3:117854/149‑1 (MQ=255)
                                                                                                                                    ataaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGata       >  2:998425/1‑149 (MQ=255)
                                                                                                                                     taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACaa                      >  3:168288/1‑133 (MQ=255)
                                                                                                                                     taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACaa                      <  4:168288/133‑1 (MQ=255)
                                                                                                                                     taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGatat      >  2:900439/1‑149 (MQ=255)
                                                                                                                                     taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGatat      >  3:280606/1‑149 (MQ=255)
                                                                                                                                     taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGatat      >  3:28396/1‑149 (MQ=255)
                                                                                                                                     taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGatat      >  3:335096/1‑149 (MQ=255)
                                                                                                                                      aaaaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATc     >  3:1159133/1‑149 (MQ=255)
                                                                                                                                      aaaaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATc     >  3:535356/1‑149 (MQ=255)
                                                                                                                                       aaaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATcc    >  1:342009/1‑149 (MQ=255)
                                                                                                                                       aaaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATcc    <  3:723889/149‑1 (MQ=255)
                                                                                                                                        aaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCa   >  1:929697/1‑149 (MQ=255)
                                                                                                                                         ttttgCCCGGCATGACGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCgcggc           <  2:348136/136‑1 (MQ=255)
                                                                                                                                         ttttgCCCGGCATGACGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAg  >  1:1425960/5‑149 (MQ=255)
                                                                                                                                         ttttgCCCGGCATGACACCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAg  >  2:285560/5‑149 (MQ=255)
                                                                                                                                               ccGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGa                           >  4:170643/1‑118 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
TCCGTTACCCGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAG  >  NC_000913/3845598‑3845883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: