Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,845,748 C→G 5.2% intergenic (+18/+28) adeD → / ← uhpT cryptic adenine deaminase/hexose phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,845,7480CG 5.2% 473.8 / 14.2 135intergenic (+18/+28)adeD/uhpTcryptic adenine deaminase/hexose phosphate transporter
Reads supporting (aligned to +/- strand):  ref base C (73/55);  new base G (4/3);  total (77/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-01

Only 100 of 136 total aligned reads displayed.

CGTTACCCGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGT  >  NC_000913/3845600‑3845895
                                                                                                                                                    |                                                                                                                                                   
 gTTACCCGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCa                                                                                                                                                    >  1:1202712/1‑149 (MQ=255)
     cccGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGcc                                                                                                                                                >  2:1419789/1‑149 (MQ=255)
      ccGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCg                                                                                                                                               >  2:842914/1‑149 (MQ=255)
      ccGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCg                                                                                                                                               >  3:862891/1‑149 (MQ=255)
      ccGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCg                                                                                                                                               >  4:808353/1‑149 (MQ=255)
       cGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCgg                                                                                                                                              <  3:324645/149‑1 (MQ=255)
        gATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCggg                                                                                                                                             <  2:760333/149‑1 (MQ=255)
             gCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCaaaa                                                                                                                                        <  2:605512/149‑1 (MQ=255)
             gCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCaaaa                                                                                                                                        <  2:134357/149‑1 (MQ=255)
              ccGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAg                                                                                                                                       >  4:1194722/1‑149 (MQ=255)
              ccGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCATGACGCCGGGcttttt                                                                                                                                       <  2:72827/149‑6 (MQ=255)
              ccGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCATGACGCCGGGcttttt                                                                                                                                       <  3:842073/149‑6 (MQ=255)
                  ttATTCAGATGGCGTTTCTTTCTCCGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCAc                                                                                                                                   <  1:1050503/149‑1 (MQ=255)
                    aTTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCa                                                                                                                                 <  2:382466/149‑1 (MQ=255)
                       cAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGtt                                                                                                                              >  2:1469254/1‑149 (MQ=255)
                        aGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTa                                                                                                                             >  1:1121191/1‑149 (MQ=255)
                        aGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTa                                                                                                                             >  1:495806/1‑149 (MQ=255)
                             gCGTTTCTTTCTCTGCCAGTGATCCCCGAGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGttt                                                                                                                        >  3:101057/1‑149 (MQ=255)
                                tttctttcTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATg                                                                                                                     <  2:30128/149‑1 (MQ=255)
                                tttctttcTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATg                                                                                                                     >  2:880685/1‑149 (MQ=255)
                                    tttctCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCAc                                                                                                                 >  3:303967/1‑149 (MQ=255)
                                     ttctCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACt                                                                                                                >  2:236438/1‑149 (MQ=255)
                                        tctGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTc                                                                                                             >  3:1198175/1‑149 (MQ=255)
                                        tctGCCAGTAATCCCCGCGCTAAAACTAGCCAGTCGGGGGCTATTTGATGGCGAGACGTTTGCCTTCACTACGCTAGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGta                                                                                                             >  4:1071709/1‑148 (MQ=255)
                                         ctGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCa                                                                                                            >  2:127472/1‑149 (MQ=255)
                                               gTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAActgct                                                                                                      >  1:130386/1‑149 (MQ=255)
                                                  aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGGCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGaa                                                                                                   <  1:1364018/149‑1 (MQ=255)
                                                  aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCa                                                                                                                                    >  4:479712/1‑116 (MQ=255)
                                                  aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCa                                                                                                                                    <  3:479712/116‑1 (MQ=255)
                                                  aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGaa                                                                                                   <  1:658841/149‑1 (MQ=255)
                                                  aTCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGaa                                                                                                   >  2:67515/1‑149 (MQ=255)
                                                     cccGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAAttt                                                                                                >  1:623207/1‑149 (MQ=255)
                                                      ccGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACTGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAAtttt                                                                                               >  4:1212679/1‑149 (MQ=255)
                                                      ccGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAAtttt                                                                                               <  3:879059/149‑1 (MQ=255)
                                                       cgcgCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAAttttt                                                                                              <  3:808353/149‑1 (MQ=255)
                                                       cgcgCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAAttttt                                                                                              <  2:495806/149‑1 (MQ=255)
                                                            tAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTtctc                                                                                         <  2:577750/149‑1 (MQ=255)
                                                                 cTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCgcggc                                                                                    >  3:1485183/1‑149 (MQ=255)
                                                                  tAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCgcggcg                                                                                   >  3:1065160/1‑149 (MQ=255)
                                                                        gTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGAtttt                                                                             <  3:942485/149‑1 (MQ=255)
                                                                         tCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTg                                                                            <  1:7212/149‑1 (MQ=255)
                                                                            ggggCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGt                                                                         <  4:1150692/149‑1 (MQ=255)
                                                                              ggCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttc                                                                       >  4:591448/1‑149 (MQ=255)
                                                                              ggCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttc                                                                       >  3:1302415/1‑149 (MQ=255)
                                                                                cTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttctt                                                                     <  1:1419789/149‑1 (MQ=255)
                                                                                 tATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttcttc                                                                    >  4:1436151/1‑149 (MQ=255)
                                                                                  aTTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTcc                                                                   <  3:1436151/149‑1 (MQ=255)
                                                                                   tttGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCa                                                                  <  4:1375829/149‑1 (MQ=255)
                                                                                       aTGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTAc                                                              >  1:1110626/1‑149 (MQ=255)
                                                                                       aTGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTAc                                                              <  3:710653/149‑1 (MQ=255)
                                                                                         ggCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTcgc                                                                                       <  2:333675/122‑1 (MQ=255)
                                                                                         ggCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTcgc                                                                                       >  1:333675/1‑122 (MQ=255)
                                                                                            gcgaAGTTTGCCTTCACTACGCTGGAAGTCCCGGAATGATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCa                                                         >  3:595314/3‑149 (MQ=255)
                                                                                                 gTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATc                                                    <  2:871011/149‑1 (MQ=255)
                                                                                                  tttGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCg                                                   >  2:1196772/1‑149 (MQ=255)
                                                                                                  attGCCATCACTACGCTCGAGGACACGAAAAAATAAAAAAAGCCTGGCGTCATGCCGGGCAAACGTCACCAGTTACGTTGATGCCACAGACCACTGCTGAATTTTTTTCTCGCGGCGGATATTGCGTTCTTCCATTACCGCCACTATCg                                                   >  4:172143/2‑149 (MQ=255)
                                                                                                     gCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAGGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCa                                                <  2:173004/149‑1 (MQ=255)
                                                                                                        ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                             >  3:625682/1‑149 (MQ=255)
                                                                                                        ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                             >  1:1048300/1‑149 (MQ=255)
                                                                                                        ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                             >  1:1392024/1‑149 (MQ=255)
                                                                                                        ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                             <  2:560395/149‑1 (MQ=255)
                                                                                                        ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                             <  1:929014/149‑1 (MQ=255)
                                                                                                        ttCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCa                                             <  2:517455/149‑1 (MQ=255)
                                                                                                          cACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAgg                                           <  2:1033198/149‑1 (MQ=255)
                                                                                                           aCTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttcttc                                                                    >  2:818021/1‑123 (MQ=255)
                                                                                                           aCTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGc                                          >  4:696273/1‑149 (MQ=255)
                                                                                                            cTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCa                                         <  2:1048300/149‑1 (MQ=255)
                                                                                                             tACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGttc                                                                       <  1:736899/118‑1 (MQ=255)
                                                                                                             tACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAg                                        >  1:298884/1‑149 (MQ=255)
                                                                                                             tACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAg                                        >  1:773665/1‑149 (MQ=255)
                                                                                                             tACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAg                                        >  2:1189945/1‑149 (MQ=255)
                                                                                                                gCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATa                                     >  4:855375/1‑149 (MQ=255)
                                                                                                                  tGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACa                                   >  2:1399986/1‑149 (MQ=255)
                                                                                                                   ggAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACaa                                  <  4:1002430/149‑1 (MQ=255)
                                                                                                                   ggAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTAAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACaa                                  <  3:890030/149‑1 (MQ=255)
                                                                                                                    gAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAAc                                 <  2:803536/149‑1 (MQ=255)
                                                                                                                          aCGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATcg                           >  1:1012238/1‑149 (MQ=255)
                                                                                                                          aCGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGCCAGATACAACCAATcg                           >  1:149327/1‑149 (MQ=255)
                                                                                                                            ggAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATcgcg                         >  1:426121/1‑149 (MQ=255)
                                                                                                                              aataatAAAAAAAGCCCGGCGTCATTCCGGGCAAAAGTCACCAGTTACTTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCtcggc                       <  2:28629/149‑1 (MQ=255)
                                                                                                                              aataatAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCgcggc                       <  3:117854/149‑1 (MQ=255)
                                                                                                                                  ataaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGata                   >  2:998425/1‑149 (MQ=255)
                                                                                                                                   taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACaa                                  >  3:168288/1‑133 (MQ=255)
                                                                                                                                   taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGatat                  >  3:335096/1‑149 (MQ=255)
                                                                                                                                   taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGatat                  >  2:1030685/1‑149 (MQ=255)
                                                                                                                                   taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGatat                  >  3:28396/1‑149 (MQ=255)
                                                                                                                                   taaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGatat                  >  3:280606/1‑149 (MQ=255)
                                                                                                                                    aaaaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATc                 >  3:535356/1‑149 (MQ=255)
                                                                                                                                     aaaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATcc                <  4:1485183/149‑1 (MQ=255)
                                                                                                                                     aaaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATcc                <  3:1194722/149‑1 (MQ=255)
                                                                                                                                     aaaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATcc                >  2:1132168/1‑149 (MQ=255)
                                                                                                                                     aaaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATcc                <  3:723889/149‑1 (MQ=255)
                                                                                                                                      aaaaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCa               >  1:929697/1‑149 (MQ=255)
                                                                                                                                       ttttgCCCGGCATGACGCCGGGCAAAAGTCCCCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTCTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAg              >  1:618139/5‑149 (MQ=255)
                                                                                                                                       ttttgCCCGGCATGACGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCgcggc                       >  1:348136/5‑140 (MQ=255)
                                                                                                                                       ttttgCCCGGCATGACGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAg              >  1:1425960/5‑149 (MQ=255)
                                                                                                                                             ccGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGa                                       >  4:170643/1‑118 (MQ=255)
                                                                                                                                             ccGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGa                                       <  3:170643/118‑1 (MQ=255)
                                                                                                                                                   tCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGt  >  4:355258/1‑149 (MQ=255)
                                                                                                                                                   tCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGt  <  2:19378/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTTACCCGATGAGCCGTTTATTCAGATGGCGTTTCTTTCTCTGCCAGTGATCCCCGCGCTAAAACTAACCAGTCAGGGGCTATTTGATGGCGAGAAGTTTGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGT  >  NC_000913/3845600‑3845895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: