BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,900,542	A→T	5.1%	I12I (ATT→ATA)	yieL ←	putative xylanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,900,542	0	A	T	5.1%	696.7 / 20.6	199	I12I (ATT→ATA)	yieL	putative xylanase
Reads supporting (aligned to +/- strand): ref base A (94/94); new base T (3/7); total (98/101)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

Only 100 of 201 total aligned reads displayed.

GACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGTTTTCCAG  >  NC_000913/3900395‑3900690

gaCATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCaa                                                                                                                                                     >  2:1402215/1‑149 (MQ=255)

 aCATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAAt                                                                                                                                                    >  3:636154/1‑149 (MQ=255)

  cATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATa                                                                                                                                                   <  2:1119554/149‑1 (MQ=255)

   ctactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAg                                                                                                                                                  >  4:625221/5‑149 (MQ=255)

    tactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGc                                                                                                                                                 >  2:1225954/4‑149 (MQ=255)

    tactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGc                                                                                                                                                 >  2:1218831/4‑149 (MQ=255)

      ttttGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCa                                                                                                                                               <  1:722592/149‑1 (MQ=255)

      ttttGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCa                                                                                                                                               >  4:1067330/1‑149 (MQ=255)

          gCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCgg                                                                                                                                           <  4:971028/149‑2 (MQ=255)

          gCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCgg                                                                                                                                           <  2:839831/149‑2 (MQ=255)

             ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaa                                                                                                                                        >  2:1301764/1‑149 (MQ=255)

             ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaa                                                                                                                                        >  3:81126/1‑149 (MQ=255)

             ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaa                                                                                                                                        <  2:949581/149‑1 (MQ=255)

             ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaa                                                                                                                                        >  3:1301818/1‑149 (MQ=255)

              cAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTTTGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaaa                                                                                                                                       <  3:649755/149‑1 (MQ=255)

              cAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaaa                                                                                                                                       <  1:299697/149‑1 (MQ=255)

               aGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGACTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAag                                                                                                                                      >  4:760806/1‑149 (MQ=255)

                 gggCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAagca                                                                                                                                    <  1:593161/149‑1 (MQ=255)

                         tAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAtttt                                                                                                                            >  2:1291834/1‑149 (MQ=255)

                         tAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAtttt                                                                                                                            <  1:128675/149‑1 (MQ=255)

                            cGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaaa                                                                                                                                       >  4:1458410/1‑135 (MQ=255)

                             gAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCGGTa                                                                                                                                         <  1:1042112/132‑1 (MQ=255)

                             gAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTtata                                                                                                                        >  2:394435/1‑149 (MQ=255)

                                 ggTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCa                                                                                                                    >  3:805124/1‑149 (MQ=255)

                                  gTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCAt                                                                                                                   >  2:498978/1‑149 (MQ=255)

                                   tCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATc                                                                                                                  <  1:621802/149‑1 (MQ=255)

                                   tCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATc                                                                                                                  <  3:978607/149‑1 (MQ=255)

                                              tCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCGATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCt                                                                                                       >  4:1400810/1‑149 (MQ=255)

                                              tCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCt                                                                                                       <  4:1195109/149‑1 (MQ=255)

                                                ggCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCttt                                                                                                     <  3:1385323/149‑1 (MQ=255)

                                                ggCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCttt                                                                                                     <  4:1002910/149‑1 (MQ=255)

                                                 gCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTc                                                                                                    <  3:695618/149‑1 (MQ=255)

                                                 gCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTc                                                                                                    >  4:790690/1‑149 (MQ=255)

                                                   aTTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCAt                                                                                                  >  3:153304/1‑149 (MQ=255)

                                                    ttGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCAtt                                                                                                 >  4:1099393/1‑149 (MQ=255)

                                                         cTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATCTTCCTTTCATTAAGtt                                                                                            >  3:1264988/1‑149 (MQ=255)

                                                         cTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGtt                                                                                            >  4:1227793/1‑149 (MQ=255)

                                                             gTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCAt                                                                                        <  3:717104/149‑1 (MQ=255)

                                                              tCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCAtt                                                                                       >  1:1188831/1‑149 (MQ=255)

                                                                acaTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTac                                                                                     <  1:56889/149‑1 (MQ=255)

                                                                 caTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTacc                                                                                    <  3:1316170/149‑1 (MQ=255)

                                                                  aTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaaa                                                                                                                                       <  2:1377074/97‑1 (MQ=255)

                                                                  aTACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaaa                                                                                                                                       >  1:1377074/1‑97 (MQ=255)

                                                                    aCTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTaccacc                                                                                 >  4:419013/1‑149 (MQ=255)

                                                                    aCTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTaccacc                                                                                 >  3:1066462/1‑149 (MQ=255)

                                                                     cTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTAccacca                                                                                <  1:267102/149‑1 (MQ=255)

                                                                     cTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTAccacca                                                                                >  4:69325/1‑149 (MQ=255)

                                                                        tttCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATAtt                                                                                                                      <  1:1145292/108‑1 (MQ=255)

                                                                        tttCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATAtt                                                                                                                      >  2:1145292/1‑108 (MQ=255)

                                                                        tttCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGAt                                                                             >  3:1187000/1‑149 (MQ=255)

                                                                        tttCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGAt                                                                             >  3:301787/1‑149 (MQ=255)

                                                                           cACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTc                                                                          <  4:1183108/149‑1 (MQ=255)

                                                                            aCCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCa                                                                         <  3:1159545/149‑1 (MQ=255)

                                                                            aCCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCa                                                                         >  3:615484/1‑149 (MQ=255)

                                                                            aCCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCa                                                                         >  2:1434680/1‑149 (MQ=255)

                                                                             ccGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAg                                                                        >  4:550270/1‑149 (MQ=255)

                                                                              cGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGc                                                                       >  1:1420006/1‑149 (MQ=255)

                                                                                 gATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTg                                                                    <  1:971994/149‑1 (MQ=255)

                                                                                  aTAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTgg                                                                   <  4:112342/149‑1 (MQ=255)

                                                                                   tAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTggg                                                                  <  3:431399/149‑1 (MQ=255)

                                                                                     gTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCa                                                                >  2:74614/1‑149 (MQ=255)

                                                                                     gTGGGTGCCGGGCTGGCTAGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCa                                                                >  1:441432/1‑149 (MQ=255)

                                                                                       gggTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCAcc                                                              >  4:278246/1‑149 (MQ=255)

                                                                                           gCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACa                                                          <  4:399430/149‑1 (MQ=255)

                                                                                             cGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGc                                                        >  1:1302089/1‑149 (MQ=255)

                                                                                                gctggctgGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCaaa                                                     <  4:1301818/149‑1 (MQ=255)

                                                                                                      tggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgtc                                               <  1:1434680/149‑1 (MQ=255)

                                                                                                      tggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgtc                                               <  3:1119994/149‑1 (MQ=255)

                                                                                                      tggcATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgtc                                               <  3:760806/149‑1 (MQ=255)

                                                                                                          atatCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCt                                           >  4:823166/1‑149 (MQ=255)

                                                                                                          atatCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCt                                           >  3:727360/1‑149 (MQ=255)

                                                                                                          atatCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCt                                           >  3:1471237/1‑149 (MQ=255)

                                                                                                            atCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCttt                                         >  3:22770/1‑149 (MQ=255)

                                                                                                              ccGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTAt                                       <  3:710132/149‑1 (MQ=255)

                                                                                                                 ctgctATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGt                                    >  4:646685/1‑149 (MQ=255)

                                                                                                                  tgctATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTc                                   <  1:1467825/149‑1 (MQ=255)

                                                                                                                    ctATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCtt                                 <  1:498978/149‑1 (MQ=255)

                                                                                                                       tGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCaa                              <  1:818562/149‑1 (MQ=255)

                                                                                                                        ggCCCACTGAGCAGAACTACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCaaa                             <  3:941301/149‑1 (MQ=255)

                                                                                                                         gCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAg                            <  2:592521/149‑1 (MQ=255)

                                                                                                                          cccACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAgg                           >  2:1017448/1‑149 (MQ=255)

                                                                                                                           ccACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGt                          <  2:116955/149‑1 (MQ=255)

                                                                                                                           ccACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATCCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGgtc                                               <  2:276010/128‑1 (MQ=255)

                                                                                                                            cACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTg                         >  1:728480/1‑149 (MQ=255)

                                                                                                                            cACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTg                         <  3:1401406/149‑1 (MQ=255)

                                                                                                                             aCTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTc                                                                                                         >  1:345444/1‑68 (MQ=255)

                                                                                                                             aCTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTc                                                                                                         <  2:345444/68‑1 (MQ=255)

                                                                                                                             aCTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGa                        <  1:759613/149‑1 (MQ=255)

                                                                                                                             aCTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGa                        >  1:161573/1‑149 (MQ=255)

                                                                                                                              cTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGaa                       <  3:402284/149‑1 (MQ=255)

                                                                                                                                   cAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCAt                  <  4:152484/149‑1 (MQ=255)

                                                                                                                                    aGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCATCGACAGCAAACTGGTCGTCTTTAtt                                      >  4:304823/1‑128 (MQ=255)

                                                                                                                                    aGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATc                 <  2:639405/149‑1 (MQ=255)

                                                                                                                                      aaaTACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGaa                       >  2:263518/1‑141 (MQ=255)

                                                                                                                                      aaaTACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCa               >  1:861264/1‑149 (MQ=255)

                                                                                                                                        aTACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGt             <  3:1442708/149‑1 (MQ=255)

                                                                                                                                             gCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGtt        <  4:955078/149‑1 (MQ=255)

                                                                                                                                              cTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGttt       <  3:870389/149‑1 (MQ=255)

                                                                                                                                               tGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGtttt      >  2:1264649/1‑149 (MQ=255)

                                                                                                                                                  cAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCCGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGTTTTCCa   <  4:392978/149‑1 (MQ=255)

GACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCAGTTCGTTTTCCAG  >  NC_000913/3900395‑3900690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`