Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,314,239 A→T 6.8% intergenic (+42/+105) yjdP → / ← phnP putative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,314,2390AT 6.8% 488.6 / 18.7 146intergenic (+42/+105)yjdP/phnPputative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (65/71);  new base T (7/3);  total (72/74)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 170 total aligned reads displayed.

TTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAAC  >  NC_000913/4314091‑4314386
                                                                                                                                                    |                                                                                                                                                   
ttGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGggga                                                                                                                                                     >  1:1457858/1‑149 (MQ=255)
  gAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggaga                                                                                                                                                   >  2:1469792/1‑149 (MQ=255)
   aaGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagag                                                                                                                                                  <  2:1453359/149‑1 (MQ=255)
    aGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtgagg                                                                                                                                                 <  3:639593/149‑1 (MQ=255)
    aGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtgagg                                                                                                                                                 <  3:1168818/149‑1 (MQ=255)
     gATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggg                                                                                                                                                <  1:702707/149‑1 (MQ=255)
     gATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggg                                                                                                                                                >  1:654165/1‑149 (MQ=255)
     gATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggg                                                                                                                                                <  2:517783/149‑1 (MQ=255)
      aTCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtgaggg                                                                                                                                                >  3:1286354/1‑148 (MQ=255)
      aTCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagaggga                                                                                                                                               >  2:787534/1‑149 (MQ=255)
      aTCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGAGGCGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCTCCCCATTGgggtgaggt                                                                                                                                                <  4:1286354/148‑2 (MQ=255)
       tCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGgggagagggag                                                                                                                                              <  3:479678/149‑1 (MQ=255)
            gcaggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAAc                                                                                                                                         <  2:1485309/149‑1 (MQ=255)
              aggcagCTGGACGATCGCTAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGa                                                                                                                                       <  1:24853/149‑1 (MQ=255)
              aggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGa                                                                                                                                       <  1:814844/149‑1 (MQ=255)
              aggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGa                                                                                                                                       <  1:442997/149‑1 (MQ=255)
              aggcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGa                                                                                                                                       <  4:330084/149‑1 (MQ=255)
                gcagCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAgc                                                                                                                                     <  1:269370/149‑1 (MQ=255)
                       gACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCaa                                                                                                                                 <  4:1237338/146‑1 (MQ=255)
                               ccagcgccagcTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATc                                                                                                                      >  2:616647/1‑149 (MQ=255)
                                 agcgccagcTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGTGCAATATTCAATATCGa                                                                                                                    <  3:705771/149‑1 (MQ=255)
                                      cagcTTGATCAGGAGCGTCGGCAAATGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGAATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGcaca                                                                                                               >  2:373565/1‑149 (MQ=255)
                                         cTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATc                                                                                                            >  4:446673/1‑149 (MQ=255)
                                            gATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAACGCAATATTCAATATCGAGCACAATCGGt                                                                                                         <  3:1056246/149‑1 (MQ=255)
                                             aTCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGa                                                                                                                                       <  2:135144/118‑1 (MQ=255)
                                                aGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTcccc                                                                                                     <  1:787534/149‑1 (MQ=255)
                                                 ggAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGAGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACACTCGGTCCCCt                                                                                                    <  3:911983/149‑1 (MQ=255)
                                                  gAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTc                                                                                                   >  1:907324/1‑149 (MQ=255)
                                                    gCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGc                                                                                                 >  4:818200/1‑149 (MQ=255)
                                                     cGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGcc                                                                                                <  1:616647/149‑1 (MQ=255)
                                                            aaCTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTgg                                                                                         >  3:223985/1‑149 (MQ=255)
                                                                 gaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                    <  4:967950/149‑1 (MQ=255)
                                                                 gaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                    <  4:486289/149‑1 (MQ=255)
                                                                 gaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                    >  3:1314612/1‑149 (MQ=255)
                                                                  aggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                   <  1:110493/149‑1 (MQ=255)
                                                                   ggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                                  <  1:125481/149‑1 (MQ=255)
                                                                   ggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                                  <  3:446673/149‑1 (MQ=255)
                                                                    gaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGANAggg                                                                                 <  3:1336847/149‑1 (MQ=255)
                                                                    gaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                 <  2:735393/149‑1 (MQ=255)
                                                                    gaTGAAGAGCGGAGAATGAAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                   >  1:440601/1‑147 (MQ=255)
                                                                       gAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                                  <  4:1313793/145‑1 (MQ=255)
                                                                         agagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAAc                                                                                                                                         <  4:658857/88‑1 (MQ=255)
                                                                         agagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAAc                                                                                                                                         >  3:658857/1‑88 (MQ=255)
                                                                         agagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                            >  2:748850/1‑149 (MQ=255)
                                                                          gagCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                           >  2:249672/1‑149 (MQ=255)
                                                                             cGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa                                                                        <  1:14295/149‑1 (MQ=255)
                                                                             cGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa                                                                        <  4:684744/149‑1 (MQ=255)
                                                                              ggAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGTAGAGGGTTAGGGTGAg                                                                       >  2:1058302/1‑149 (MQ=255)
                                                                              ggAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGtt                                                                               <  3:981253/141‑1 (MQ=255)
                                                                              ggAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                       <  1:1369908/149‑1 (MQ=255)
                                                                              ggAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                       <  1:131993/149‑1 (MQ=255)
                                                                              ggAGAATGGAAGATGAGTATGGGCAATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCCCCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                       <  4:1303709/149‑1 (MQ=255)
                                                                               gagaATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGggga                                                                                                                                                     <  4:926835/70‑1 (MQ=255)
                                                                               gagaATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGggga                                                                                                                                                     >  3:926835/1‑70 (MQ=255)
                                                                                   aTGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATCTCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGc                                                                  >  4:615477/1‑149 (MQ=255)
                                                                                   aTGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGc                                                                  <  4:47170/149‑1 (MQ=255)
                                                                                     ggAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCa                                                                <  3:245757/149‑1 (MQ=255)
                                                                                            gAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGca                                                         >  2:236593/1‑149 (MQ=255)
                                                                                                aTGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACAtt                                                     <  2:379782/149‑1 (MQ=255)
                                                                                                 tGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTc                                                    <  1:146741/149‑1 (MQ=255)
                                                                                                  gggCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTcc                                                   <  1:236593/149‑1 (MQ=255)
                                                                                                   ggCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTccc                                                  <  3:380315/149‑1 (MQ=255)
                                                                                                      gATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCctct                                               >  4:986385/1‑149 (MQ=255)
                                                                                                        tGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCtca                                             <  4:1103983/149‑1 (MQ=255)
                                                                                                        tGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCtca                                             >  2:839549/1‑149 (MQ=255)
                                                                                                            gTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCctc                                                                                            <  3:1378812/98‑1 (MQ=255)
                                                                                                            gTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGtt                                                                               >  1:985527/1‑111 (MQ=255)
                                                                                                            gTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGtt                                                                               <  2:985527/111‑1 (MQ=255)
                                                                                                            gTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCAc                                         >  1:504128/1‑149 (MQ=255)
                                                                                                                 gTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCAc                                    >  2:666843/1‑149 (MQ=255)
                                                                                                                  tATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                                    >  1:322689/1‑116 (MQ=255)
                                                                                                                  tATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCAcc                                   <  3:377175/149‑1 (MQ=255)
                                                                                                                  tATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCAcc                                   >  1:1077020/1‑149 (MQ=255)
                                                                                                                  tATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCAcc                                   >  4:204153/1‑149 (MQ=255)
                                                                                                                  tATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCAcc                                   >  2:250705/1‑149 (MQ=255)
                                                                                                                        gCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAtc                             >  3:780698/1‑149 (MQ=255)
                                                                                                                         cAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAtct                            >  4:649610/1‑149 (MQ=255)
                                                                                                                            gCATCGCCCCTCTCCCCAATGGGGAGGGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATAGGTCCCCTCGCCCCTCTGGGGAGGGGGTTAGGGTGAGGGGGACACCGCGCACACTCCCTCTCATCACGCCACCCCAATCTcca                         >  2:840305/1‑149 (MQ=38)
                                                                                                                                  tcccTCTCCCCATTTGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccat                   >  3:106843/2‑149 (MQ=38)
                                                                                                                                  tcccTCTCCCCATTGGGGTGAGGGGGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                                  >  1:350589/2‑86 (MQ=37)
                                                                                                                                  tcccTCTCCCCATTGGGGTGAGGGGGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                                  <  2:350589/85‑1 (MQ=37)
                                                                                                                                  ccccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccat                   >  1:43675/1‑149 (MQ=255)
                                                                                                                                   ccttCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccatc                  <  1:718190/146‑1 (MQ=255)
                                                                                                                                   cccTCTCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatcccatc                  >  3:684973/1‑149 (MQ=255)
                                                                                                                                    ccTCTCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCa                 >  3:508646/1‑149 (MQ=255)
                                                                                                                                    ccTCTCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCa                 >  4:404694/1‑149 (MQ=255)
                                                                                                                                     cTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCAc                                         >  4:348762/1‑124 (MQ=255)
                                                                                                                                       cACCCAAATCGGGAGAGGTAGAACACGAACGCAATATTCAATATCGAGCACAAGCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCTACCCCAATCTCCATCCCATCAAAc              <  3:1251391/149‑1 (MQ=38)
                                                                                                                                         ccccAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                   >  4:1232392/1‑78 (MQ=255)
                                                                                                                                         ccccAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                   <  3:1232392/78‑1 (MQ=255)
                                                                                                                                          cccATTGGGGTGAGGGAGAAAACGAGCGCAATATTCACTATCGAGCACAATCGGTCCCCTCGCCCCTCTgg                                                                                         >  2:713689/1‑71 (MQ=38)
                                                                                                                                          cccAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTg                                                                                          >  1:21964/1‑70 (MQ=255)
                                                                                                                                          cccAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAcccc           >  2:1225684/1‑149 (MQ=255)
                                                                                                                                          cccAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAcccc           >  3:28162/1‑149 (MQ=255)
                                                                                                                                          cccAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGAGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAcccc           >  1:976140/1‑149 (MQ=255)
                                                                                                                                            cAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGGGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCAc         >  2:281528/1‑149 (MQ=255)
                                                                                                                                             aaTGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCAcc        >  2:1357698/1‑149 (MQ=255)
                                                                                                                                                  ggagagggagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCAc         >  2:630278/1‑143 (MQ=255)
                                                                                                                                                  ggagagggagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCaaa   <  1:1058302/149‑1 (MQ=255)
                                                                                                                                                   gagagggagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAAc  <  3:1204314/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGAAGATCGCCGCAGGCAGCTGGACGATCGCCAGCGCCAGCTTGATCAGGAGCGTCGGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAAC  >  NC_000913/4314091‑4314386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: