Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,395,455 G→A 8.8% A464T (GCA→ACA)  nnr → bifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,395,4550GA 8.8% 423.5 / 22.3 136A464T (GCA→ACA) nnrbifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
Reads supporting (aligned to +/- strand):  ref base G (60/64);  new base A (8/4);  total (68/68)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 137 total aligned reads displayed.

AACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATT  >  NC_000913/4395310‑4395602
                                                                                                                                                 |                                                                                                                                                   
aaCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAg                                                                                                                                                  <  3:463468/149‑1 (MQ=255)
  ccGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGc                                                                                                                                                <  2:1136947/149‑1 (MQ=255)
   cGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCt                                                                                                                                               <  2:993798/149‑1 (MQ=255)
     tGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt                                                                                                                                             <  1:331810/149‑1 (MQ=255)
     tGGTCGCAGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt                                                                                                                                             <  2:23095/149‑1 (MQ=255)
             gccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCa                                                                                                                                     >  3:56482/1‑149 (MQ=255)
               ccATCCTGACGCTTTAGGCTTTATTGATGCCGTAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTGTGGTATTATTGGTGTATTGCTTGGCCAAAGACTGTCGCCGTATGTTGCATCCTGTGCAGGCTGTGTCGCGCACg                                                                                                                                   >  4:686134/1‑149 (MQ=255)
               ccATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACg                                                                                                                                   >  3:669469/1‑149 (MQ=255)
                   ccTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgc                                                                                                                                        >  2:1394588/1‑140 (MQ=255)
                   ccTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGc                                                                                                                               <  3:752911/149‑1 (MQ=255)
                         gCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGc                                                                                                                         >  4:223205/1‑149 (MQ=255)
                         gCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGc                                                                                                                         >  1:1094795/1‑149 (MQ=255)
                          cTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCAGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTCTGCAGGCTGTGTCGCGCACGGTGCGGCAGCt                                                                                                                        >  1:1418889/1‑149 (MQ=255)
                            ttAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGa                                                                                                                      <  4:1186825/149‑1 (MQ=255)
                             tAGGCATTATTGATGGCGGAAATGCCGGTCTGGCGAGCGGCGGCATGGGCGATCTGATCTCTGGTATTATTCGCGCATTGATTGAGGAAAAACTCCCGACATATGATACGGCATGTGCAGGATGTGTAGCGCACGGTGCGGCAGATGaa                                                                                                                     <  4:913897/149‑2 (MQ=255)
                             tAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGAc                                                                                                                     >  2:172558/1‑149 (MQ=255)
                               ggCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGt                                                                                                                   >  1:962484/1‑149 (MQ=255)
                               ggCAATATTGATGCCGGAAATGCAGGCATGGCGAGCGGGGGCATGGGCGATGTGCTCTCTCGTATTATTGGCGCATTGCTTGGGCAAAAATTGTCGCCGTATGATTCAGCCTGTGCAAGCTGTGTCGCGCACGGTGCGGCAGCTGACGt                                                                                                                   >  4:214646/1‑149 (MQ=255)
                                 cATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTAc                                                                                                                 >  4:571344/1‑149 (MQ=255)
                                        gATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCgg                                                                                                                             >  1:100285/1‑130 (MQ=255)
                                        gATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCgg                                                                                                                             <  2:100285/130‑1 (MQ=255)
                                        gATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTggcggc                                                                                                          >  1:851987/1‑149 (MQ=255)
                                          tGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGgcgc                                                                                                        <  3:223205/149‑1 (MQ=255)
                                           gCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGcgcg                                                                                                       >  2:173903/1‑149 (MQ=255)
                                            ccGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGt                                                                                                      >  2:698209/1‑149 (MQ=255)
                                               gAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTGTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGGTGACGTACTGGCGGCGCGtttt                                                                                                   >  3:907397/1‑149 (MQ=255)
                                                aaaTGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTg                                                                                                  <  1:873407/149‑1 (MQ=255)
                                                aaaTGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTg                                                                                                  <  3:938186/149‑1 (MQ=255)
                                                aaaTGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTg                                                                                                  <  2:917559/149‑1 (MQ=255)
                                                aaaTGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTg                                                                                                  <  3:1042376/149‑1 (MQ=255)
                                                  aTGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGgcgc                                                                                                        >  4:602302/1‑141 (MQ=255)
                                                  aTGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGgcgc                                                                                                        <  3:602302/141‑1 (MQ=255)
                                                            ggCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGa                                                                                      <  2:227307/149‑1 (MQ=255)
                                                                 gcggcgGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTg                                                                                 <  1:1215599/149‑1 (MQ=255)
                                                                       gcATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAAcc                                                                           <  4:319662/149‑1 (MQ=255)
                                                                       gcATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAAcc                                                                           <  1:696049/149‑1 (MQ=255)
                                                                       gcATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAAcc                                                                           <  2:283446/149‑1 (MQ=255)
                                                                           gggCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGCACAGGCtgc                                                                                                                                             >  4:1449580/1‑78 (MQ=255)
                                                                                aTGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCttt                                                                  <  1:430736/149‑1 (MQ=255)
                                                                                aTGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCttt                                                                  <  1:520529/149‑1 (MQ=255)
                                                                                 tgtgCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCtttt                                                                 <  4:431393/149‑1 (MQ=255)
                                                                                    gCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACAGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCa                                                              >  2:1460011/1‑149 (MQ=255)
                                                                                      tctctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACg                                                            >  1:607260/1‑149 (MQ=255)
                                                                                      tctctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACg                                                            >  4:618230/1‑149 (MQ=255)
                                                                                      tctctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACg                                                            <  1:188000/149‑1 (MQ=255)
                                                                                        tctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCt                                                          >  2:185786/1‑149 (MQ=255)
                                                                                        tctGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCt                                                          >  4:405492/1‑149 (MQ=255)
                                                                                          tGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGa                                                                         >  4:806141/1‑132 (MQ=255)
                                                                                          tGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGa                                                                         <  3:806141/132‑1 (MQ=255)
                                                                                            gTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACAGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAg                                                      <  1:1460011/149‑1 (MQ=255)
                                                                                             tattatTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGc                                                     >  1:630876/1‑149 (MQ=255)
                                                                                             tattatTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGc                                                     <  2:630876/149‑1 (MQ=255)
                                                                                                tattGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTa                                                  <  3:1263920/149‑1 (MQ=255)
                                                                                                tattGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTa                                                  <  3:237117/149‑1 (MQ=255)
                                                                                                  ttGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTAtt                                                <  3:716071/149‑1 (MQ=255)
                                                                                                       gcATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTaa                                           <  3:398406/149‑1 (MQ=255)
                                                                                                        ccttGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCATGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAc                                          <  4:1273059/147‑1 (MQ=255)
                                                                                                        cATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAc                                          <  1:173903/149‑1 (MQ=255)
                                                                                                         aTTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAcc                                         <  1:1026309/149‑1 (MQ=255)
                                                                                                         aTTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAcc                                         >  4:366378/1‑149 (MQ=255)
                                                                                                         aTTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAcc                                         >  1:124413/1‑149 (MQ=255)
                                                                                                            gCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCgg                                      <  2:1035130/149‑1 (MQ=255)
                                                                                                             cTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCCACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGa                                     >  2:110/1‑149 (MQ=255)
                                                                                                              ttGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGaa                                    <  3:459575/149‑1 (MQ=255)
                                                                                                              ttGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGaa                                    <  3:561607/149‑1 (MQ=255)
                                                                                                              ttGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGaa                                    <  4:843418/149‑1 (MQ=255)
                                                                                                                gggCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGt                                  >  2:308095/1‑149 (MQ=255)
                                                                                                                  gCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGa                                >  1:908517/1‑149 (MQ=255)
                                                                                                                   cAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGAc                               <  2:621003/149‑1 (MQ=255)
                                                                                                                   cAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGAc                               >  3:825267/1‑149 (MQ=255)
                                                                                                                    aaaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACt                              <  1:6423/149‑1 (MQ=255)
                                                                                                                    aaaaaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACt                              >  1:743645/1‑149 (MQ=255)
                                                                                                                       aaCTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGAt                           >  2:702885/1‑149 (MQ=255)
                                                                                                                            tCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAc                                          <  2:848037/129‑1 (MQ=255)
                                                                                                                              gCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAAcc                    <  4:705165/149‑1 (MQ=255)
                                                                                                                              gCCGTATGATGCAGCCTGTACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAAcc                    >  3:1434451/1‑149 (MQ=255)
                                                                                                                               ccGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCa                   <  4:96849/149‑1 (MQ=255)
                                                                                                                                cGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCat                  <  3:1135018/149‑1 (MQ=255)
                                                                                                                                  tATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCatga                >  2:1455476/1‑149 (MQ=255)
                                                                                                                                   gcgacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGGTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCatgat               >  4:1085618/7‑149 (MQ=255)
                                                                                                                                   atgatgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCatgat               <  4:1042390/149‑1 (MQ=255)
                                                                                                                                   atgatgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCatgat               <  4:56482/149‑1 (MQ=255)
                                                                                                                                   atgatgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCatgat               <  1:702885/149‑1 (MQ=255)
                                                                                                                                     gatgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCAtgatga             >  4:86029/1‑149 (MQ=255)
                                                                                                                                     gatgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCAtgatga             >  3:285971/1‑149 (MQ=255)
                                                                                                                                     gacacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCAtgatga             >  3:1144699/5‑149 (MQ=255)
                                                                                                                                       tgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAAt           >  4:1297904/1‑149 (MQ=255)
                                                                                                                                       tgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAAt           >  1:233436/1‑149 (MQ=255)
                                                                                                                                       tgCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAAt           >  4:28140/1‑149 (MQ=255)
                                                                                                                                       cacAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTa                                                  <  1:253283/108‑1 (MQ=255)
                                                                                                                                        acAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCttt                                                                  >  4:1137140/2‑93 (MQ=255)
                                                                                                                                        acAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCttt                                                                  <  3:1137140/92‑1 (MQ=255)
                                                                                                                                        acAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATc          >  4:575259/2‑149 (MQ=255)
                                                                                                                                        acAGCCTGCACAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATc          >  4:664550/2‑149 (MQ=255)
                                                                                                                                          aGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCCCGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGa        <  1:159283/149‑1 (MQ=255)
                                                                                                                                          aGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGa        >  1:341631/1‑149 (MQ=255)
                                                                                                                                           gCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAg       <  1:308095/149‑1 (MQ=255)
                                                                                                                                            ccTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGt      >  1:1277420/1‑149 (MQ=255)
                                                                                                                                            ccTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGt      >  1:1409683/1‑149 (MQ=255)
                                                                                                                                                tgCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAAtt  <  2:528643/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACGGTGCGGCAGCTGACGTACTGGCGGCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATT  >  NC_000913/4395310‑4395602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: