BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,638,691	A→G	6.9%	T172A (ACC→GCC)	creD →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,691	0	A	G	6.9%	441.6 / 18.0	144	T172A (ACC→GCC)	creD	inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (65/69); new base G (3/7); total (68/76)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

Only 100 of 145 total aligned reads displayed.

TTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATG  >  NC_000913/4638543‑4638837

tttCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCa                                                                                                                                                    >  1:1353660/1‑149 (MQ=255)

    gATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGTTTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCgg                                                                                                                                                >  4:1014298/1‑149 (MQ=255)

    gATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCgg                                                                                                                                                <  4:1305061/149‑1 (MQ=255)

    gATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCgg                                                                                                                                                >  4:617208/1‑149 (MQ=255)

    gATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCgg                                                                                                                                                <  1:1400401/149‑1 (MQ=255)

    gATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTAGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCgg                                                                                                                                                >  3:1404098/1‑149 (MQ=255)

      tGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGt                                                                                                                                              >  3:1273794/1‑149 (MQ=255)

          tCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGTGCCGGGttcaa                                                                                                                                          <  2:1406285/149‑4 (MQ=255)

          tCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTaga                                                                                                                                          <  1:1003062/149‑1 (MQ=255)

           cgcgTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGTGCCGGGttcaat                                                                                                                                         <  1:1493871/149‑5 (MQ=255)

            gcgTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGTGCCGGGttcaatg                                                                                                                                        <  3:1098350/149‑6 (MQ=255)

            gcgTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGTGCCGGGttcaatg                                                                                                                                        <  4:26127/149‑6 (MQ=255)

            gcgTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGTGCCGGGttcaatg                                                                                                                                        <  1:613976/149‑6 (MQ=255)

            gcgTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGc                                                                                                                                        >  2:130345/1‑149 (MQ=255)

               tCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGTGCCGGGttcaatg                                                                                                                                        >  2:1181710/1‑141 (MQ=255)

               tCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAg                                                                                                                                     <  3:777406/149‑1 (MQ=255)

               tCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAg                                                                                                                                     <  4:771507/149‑1 (MQ=255)

                  tAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCg                                                                                                                                  <  4:1227170/149‑1 (MQ=255)

                   aGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCgg                                                                                                                                 >  1:143464/1‑149 (MQ=255)

                    gCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCggg                                                                                                                                >  3:832124/1‑149 (MQ=255)

                    gCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCggg                                                                                                                                >  4:869569/1‑149 (MQ=255)

                      gAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCa                                                                                                                              <  4:1150288/149‑1 (MQ=255)

                         cTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGTGCCGGGttcaat                                                                                                                                         <  3:905902/135‑5 (MQ=255)

                            aaCGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGt                                                                                                                        >  1:682264/1‑149 (MQ=255)

                            aaCGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCAGGGATGCGCGTGATATTGGTGTGGTGAAAGCGCCTGAAGTTAACGCAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGt                                                                                                                        >  4:1086708/1‑149 (MQ=255)

                              cgcgCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTACCGGAACGGCGCTGACCATTGACCCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGc                                                                                                                      <  1:1197075/149‑1 (MQ=255)

                              cgcgCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGc                                                                                                                      <  1:1032853/149‑1 (MQ=255)

                              cgcgCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCATGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGc                                                                                                                      <  3:274567/149‑1 (MQ=255)

                                 gcCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCata                                                                                                                   >  1:195835/1‑149 (MQ=255)

                                  ccAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCatat                                                                                                                  <  4:48029/149‑1 (MQ=255)

                                     aaTATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATccc                                                                                                               >  3:140457/1‑149 (MQ=255)

                                       tatCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCtt                                                                                                             >  2:438852/1‑149 (MQ=255)

                                          cACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTAc                                                                                                          <  1:998626/149‑1 (MQ=255)

                                           aCCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTAcc                                                                                                         <  3:1228189/149‑1 (MQ=255)

                                             cTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTg                                                                                                       <  4:1149567/149‑1 (MQ=255)

                                              ttAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGa                                                                                                      >  4:257299/1‑149 (MQ=255)

                                                    aaGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTTAAGGGGa                                                                                                <  4:210121/149‑1 (MQ=255)

                                                       ccATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTg                                                                                             >  1:1467841/1‑149 (MQ=255)

                                                        cATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTgg                                                                                            <  4:756075/149‑1 (MQ=255)

                                                         aTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGc                                                                                           <  1:741857/149‑1 (MQ=255)

                                                             aTTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTCGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGaa                                                                                       <  1:284993/149‑1 (MQ=255)

                                                                gtgATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCa                                                                                    <  1:1149558/149‑1 (MQ=255)

                                                                  gATTAGCGTCGGGGATGCGCGTGGGATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGTTCACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGt                                                                                                                        <  4:910530/111‑1 (MQ=255)

                                                                    ttAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAAc                                                                                >  3:497803/1‑149 (MQ=255)

                                                                          tCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAg                                                                          <  2:1253509/149‑1 (MQ=255)

                                                                            ggggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGt                                                                                                                        >  2:661094/1‑101 (MQ=255)

                                                                            ggggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGt                                                                                                                        <  1:661094/101‑1 (MQ=255)

                                                                            ggggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGaa                                                                                                     >  3:1060984/1‑120 (MQ=255)

                                                                            ggggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGa                                                                                                >  1:385849/1‑125 (MQ=255)

                                                                            ggggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCt                                                                        <  2:675700/149‑1 (MQ=255)

                                                                             gggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAg                                                                                                    >  1:181788/1‑120 (MQ=255)

                                                                              ggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCaa                                                                                                                                      >  2:980419/1‑85 (MQ=255)

                                                                              ggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCaa                                                                                                                                      <  1:980419/85‑1 (MQ=255)

                                                                               gATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGaa                                                                     <  4:1461246/149‑1 (MQ=255)

                                                                                aTGCGCGTGGTATTGGTGTGGTGAAAGCGCGTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAat                                                                    <  2:1085933/149‑1 (MQ=255)

                                                                                  gcgcGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGTCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAatat                                                                  <  4:501045/149‑1 (MQ=255)

                                                                                     cgTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTGCCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGc                                                               <  4:550502/149‑1 (MQ=255)

                                                                                      gTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGcc                                                              <  4:355252/149‑1 (MQ=255)

                                                                                          tATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGc                                                                                                                                   <  2:800289/76‑1 (MQ=255)

                                                                                            ttGGTGTGGTGAAAGCGCCTGAAGTTAGCGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATTCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAAt                                                        <  2:1221847/149‑1 (MQ=255)

                                                                                            ttGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGTCCCTGAAt                                                        >  2:1351374/1‑149 (MQ=255)

                                                                                                tgtggtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTaa                                                    <  1:322658/149‑1 (MQ=255)

                                                                                                tgtggtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTaa                                                    >  2:1380763/1‑149 (MQ=255)

                                                                                                   ggtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCg                                                 <  3:1254202/149‑1 (MQ=255)

                                                                                                    gtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAgg                                                                                                                                    <  2:1095777/65‑1 (MQ=255)

                                                                                                    gtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAgg                                                                                                                                    >  1:1095777/1‑65 (MQ=255)

                                                                                                    gtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCgg                                                >  2:1510613/1‑149 (MQ=255)

                                                                                                         aGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCg                                           >  3:28950/1‑149 (MQ=255)

                                                                                                          gcgcCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCgg                                          >  1:801321/1‑149 (MQ=255)

                                                                                                            gcCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCg                                        >  4:1351025/1‑149 (MQ=255)

                                                                                                               tGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATc                                     <  3:257299/149‑1 (MQ=255)

                                                                                                               tGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATc                                     >  3:469440/1‑149 (MQ=255)

                                                                                                                    ttAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGACGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCt                                >  3:328028/1‑149 (MQ=255)

                                                                                                                    ttAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCt                                >  3:1029795/1‑149 (MQ=255)

                                                                                                                    ttAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCt                                >  2:874060/1‑149 (MQ=255)

                                                                                                                    ttAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCACTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCt                                <  4:52269/149‑1 (MQ=255)

                                                                                                                      aacggaacggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCtgt                              <  1:337613/149‑1 (MQ=255)

                                                                                                                      aacggaacggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCtgt                              >  1:115540/1‑149 (MQ=255)

                                                                                                                         ggaacggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTgtggt                           >  3:650119/1‑149 (MQ=255)

                                                                                                                         ggaacggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTgtggt                           >  2:521033/1‑149 (MQ=255)

                                                                                                                              ggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGCGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAAt                                                        >  4:922655/1‑115 (MQ=255)

                                                                                                                              ggCGCTCACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAAt                                                        <  3:922655/115‑1 (MQ=255)

                                                                                                                                   tGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTggcggg                 <  2:242860/149‑1 (MQ=255)

                                                                                                                                   tGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTggcggg                 >  2:961626/1‑149 (MQ=255)

                                                                                                                                   tGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTggcggg                 <  3:869569/149‑1 (MQ=255)

                                                                                                                                    gACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATg                                                                 <  4:350686/100‑1 (MQ=255)

                                                                                                                                    gACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATg                                                                 >  3:350686/1‑100 (MQ=255)

                                                                                                                                    gACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTggcgggc                <  1:1380763/149‑1 (MQ=255)

                                                                                                                                     aCCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTggcgggcg               >  4:1075014/1‑149 (MQ=255)

                                                                                                                                      ccATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGt              <  4:1232473/149‑1 (MQ=255)

                                                                                                                                      ccATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGt              >  3:1249671/1‑149 (MQ=255)

                                                                                                                                       cATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCtg                               >  3:1299667/1‑131 (MQ=255)

                                                                                                                                       cATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCtg                               <  4:1299667/131‑1 (MQ=255)

                                                                                                                                       cATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTa             >  2:1496764/1‑149 (MQ=255)

                                                                                                                                       cATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTa             >  2:331933/1‑149 (MQ=255)

                                                                                                                                        aTTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTaa            >  4:826688/1‑149 (MQ=255)

                                                                                                                                          tGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATa          <  1:561009/149‑1 (MQ=255)

                                                                                                                                           gAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAg         <  2:458013/149‑1 (MQ=255)

                                                                                                                                             aCCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCg       >  3:1110612/1‑149 (MQ=255)

                                                                                                                                               ccGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCtgtg                             <  1:930145/125‑1 (MQ=255)

TTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATG  >  NC_000913/4638543‑4638837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`