Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,522,716 T→A 5.5% D117V (GAC→GTC)  yncD ← putative iron outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,522,7160TA 5.5% 535.4 / 15.8 157D117V (GAC→GTC) yncDputative iron outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (70/78);  new base A (3/6);  total (73/84)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 158 total aligned reads displayed.

TTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCA  >  NC_000913/1522568‑1522863
                                                                                                                                                    |                                                                                                                                                   
   aaTGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCgg                                                                                                                                                  >  2:790202/1‑149 (MQ=255)
      ggttggtGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCgg                                                                                                                                                  >  1:115446/1‑146 (MQ=255)
      ggttggtGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCgg                                                                                                                                                  <  2:115446/146‑1 (MQ=255)
        ttggtGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGACGGGCaag                                                                                                                                             <  4:495836/149‑3 (MQ=255)
             ggCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGc                                                                                                                                        <  1:790202/149‑1 (MQ=255)
             ggCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGc                                                                                                                                        <  2:685262/149‑1 (MQ=255)
             ggCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGc                                                                                                                                        >  4:182354/1‑149 (MQ=255)
              gCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCATGCCCGACGGGCAagggcaaa                                                                                                                                       <  1:1108609/149‑6 (MQ=255)
               cTGTTGTCCGGTCTGGGAGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTCGATGTTTGCCCTTGCCCGTCGGGCATTGTGGCgg                                                                                                                                      <  4:445355/149‑1 (MQ=255)
                  ttgtCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGACGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAACTCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGaa                                                                                                                                   <  4:1233178/149‑1 (MQ=255)
                  ttggCCAGTCTGGGTGGATATAGTCATTCACCCACCGGATCCGTTGCCATTCAGGGCAGAGTAGGGGCCACGCAGCACGTCCACATTTTGGACACCGCTTCAATCCATGTTGGAGGGTTGCCCTTGCCCGTCGGGCATGGTGGCGGGaa                                                                                                                                   <  2:525921/149‑1 (MQ=255)
                    gtCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCATGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATa                                                                                                                                 <  3:574032/149‑1 (MQ=255)
                    gtCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATa                                                                                                                                 >  2:1445420/1‑149 (MQ=255)
                      ccGGTCTGGGGGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATAcc                                                                                                                               <  2:1341701/149‑1 (MQ=255)
                         gTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTc                                                                                                                            <  3:1259849/149‑1 (MQ=255)
                          tCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTcc                                                                                                                           >  1:311903/1‑149 (MQ=255)
                                  gtgACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAg                                                                                                                   >  3:529842/1‑149 (MQ=255)
                                   tgACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAgg                                                                                                                  <  4:1349454/149‑1 (MQ=255)
                                    gACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgc                                                                                                                 <  4:1019945/149‑1 (MQ=255)
                                    gACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgc                                                                                                                 <  3:1396691/149‑1 (MQ=255)
                                      cattcattACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcga                                                                                                               <  2:311903/149‑1 (MQ=255)
                                       attcattACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCAtg                                                                                                                                             <  1:1109406/118‑1 (MQ=255)
                                       attcattACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCAtg                                                                                                                                             >  2:1109406/1‑118 (MQ=255)
                                       attcattACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaa                                                                                                              >  1:546636/1‑149 (MQ=255)
                                           attACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaatacc                                                                                                          >  3:896767/1‑149 (MQ=255)
                                           attACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaatacc                                                                                                          >  1:1503435/1‑149 (MQ=255)
                                             tACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaataccgc                                                                                                        >  1:1140818/1‑149 (MQ=255)
                                              aCCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTc                                                                                                                                                    >  2:1205152/1‑104 (MQ=255)
                                              aCCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTc                                                                                                                                                    <  1:1205152/104‑1 (MQ=255)
                                                 ccaccaGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaataccgc                                                                                                        <  4:872537/145‑1 (MQ=255)
                                                  caccaGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaataccgcgaata                                                                                                   >  3:602071/1‑149 (MQ=255)
                                                       gACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgc                                                                                                                 >  4:574551/1‑130 (MQ=255)
                                                        aCGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTaa                                                                                             >  1:606701/1‑149 (MQ=255)
                                                                 cATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCgg                                                                                    >  3:4611/1‑149 (MQ=255)
                                                                   tACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGa                                                                                  <  4:444140/149‑1 (MQ=255)
                                                                     cAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGc                                                                                <  4:1455645/149‑1 (MQ=255)
                                                                      aGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGcc                                                                               >  1:1383892/1‑149 (MQ=255)
                                                                        ggCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCaa                                                                             >  3:1446860/1‑149 (MQ=255)
                                                                         gCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCaaa                                                                            >  1:183276/1‑149 (MQ=255)
                                                                                   ggCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATc                                                                  >  3:425871/1‑149 (MQ=255)
                                                                                   ggCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATc                                                                  <  4:4611/149‑1 (MQ=255)
                                                                                   ggCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATc                                                                  <  2:546636/149‑1 (MQ=255)
                                                                                    gCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGTGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCg                                                                 >  3:1411114/1‑149 (MQ=255)
                                                                                      cACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaatacc                                                                                                          <  1:904064/106‑1 (MQ=255)
                                                                                       aCGCAGCACTTCCACATTTTGCACACTGCTTACATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACa                                                              <  2:224204/149‑1 (MQ=255)
                                                                                       aCGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACa                                                              <  2:842750/149‑1 (MQ=255)
                                                                                         gcagcaCTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGc                                                            >  2:392595/1‑149 (MQ=255)
                                                                                                  ccACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCt                                                   >  4:1421214/1‑149 (MQ=255)
                                                                                                   cacaTTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGTCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGACCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCtt                                                  >  4:1373887/1‑149 (MQ=255)
                                                                                                   cacaTTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCtt                                                  >  4:1351835/1‑149 (MQ=255)
                                                                                                   cacaTTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCtt                                                  >  1:21904/1‑149 (MQ=255)
                                                                                                   cacaTTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCtt                                                  <  2:40451/149‑1 (MQ=255)
                                                                                                    acaTTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGa                                                                >  4:290361/1‑134 (MQ=255)
                                                                                                    acaTTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTg                                                 >  2:1053098/1‑149 (MQ=255)
                                                                                                     caTTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTgc                                                <  4:1037178/149‑1 (MQ=255)
                                                                                                     caTTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTgc                                                <  3:1331691/149‑1 (MQ=255)
                                                                                                     caTTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAACCTTgc                                                <  1:431278/149‑1 (MQ=255)
                                                                                                       ttttGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGcc                                                                               <  1:675497/116‑1 (MQ=255)
                                                                                                       ttttGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGcc                                                                               >  2:675497/1‑116 (MQ=255)
                                                                                                       ttttGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTgcgc                                              >  2:347983/1‑149 (MQ=255)
                                                                                                             acacTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGtt                                        >  1:1233701/1‑149 (MQ=255)
                                                                                                               acTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCt                                      >  1:194564/1‑149 (MQ=255)
                                                                                                                 tGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGc                                    <  3:24688/149‑1 (MQ=255)
                                                                                                                    ttAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCgg                                 >  3:1405731/1‑149 (MQ=255)
                                                                                                                    ttAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCgg                                 >  3:576794/1‑149 (MQ=255)
                                                                                                                        aTCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGtttt                             <  1:535342/149‑1 (MQ=255)
                                                                                                                        aTCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGtttt                             >  2:616679/1‑149 (MQ=255)
                                                                                                                        aTCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGtttt                             <  2:839434/149‑1 (MQ=255)
                                                                                                                        aTCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGtttt                             >  4:21816/1‑149 (MQ=255)
                                                                                                                        aTCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGtttt                             <  3:1351835/149‑1 (MQ=255)
                                                                                                                           gATGTTGGATGTTTGCCGTTGCCCGTGGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCTGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCAGAGTTCTGCCGGTTTTGTa                          <  2:1159173/149‑1 (MQ=255)
                                                                                                                           gATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTa                          <  4:309280/149‑1 (MQ=255)
                                                                                                                            aTGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTAc                         <  2:998804/149‑1 (MQ=255)
                                                                                                                             tGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTg                                                 >  2:1371721/1‑124 (MQ=255)
                                                                                                                              gTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCt                       <  3:509741/149‑1 (MQ=255)
                                                                                                                               ttGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTg                      <  4:664650/149‑1 (MQ=255)
                                                                                                                               ttGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTg                      >  2:1452310/1‑149 (MQ=255)
                                                                                                                                 ggATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTgc                                                >  3:705417/1‑121 (MQ=255)
                                                                                                                                 ggATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTgc                                                <  4:705417/121‑1 (MQ=255)
                                                                                                                                 ggATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCa                    >  3:453369/1‑149 (MQ=255)
                                                                                                                                      tttGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCtt                                                  >  4:1071613/1‑114 (MQ=255)
                                                                                                                                      tttGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCtt                                                  <  3:1071613/114‑1 (MQ=255)
                                                                                                                                      tttGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCa               <  1:1473424/149‑1 (MQ=255)
                                                                                                                                      tttGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCa               <  3:122738/149‑1 (MQ=255)
                                                                                                                                      tttGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCa               <  3:459704/149‑1 (MQ=255)
                                                                                                                                        tgcccttgcccGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAgg             >  2:1470649/1‑149 (MQ=255)
                                                                                                                                        tgcccttgcccGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAgg             <  3:917309/149‑1 (MQ=255)
                                                                                                                                        tgcccttgcccGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAgg             <  3:287600/149‑1 (MQ=255)
                                                                                                                                         gcccttgcccGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGa                                                                <  2:98119/97‑1 (MQ=255)
                                                                                                                                         gcccttgcccGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGa                                                                >  1:98119/1‑97 (MQ=255)
                                                                                                                                         caccatgcccGACGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAAcc                >  2:423330/6‑145 (MQ=255)
                                                                                                                                          accatgcccGACGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCa           >  4:592046/5‑149 (MQ=255)
                                                                                                                                             ttgcccGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTAc                         <  2:460099/132‑1 (MQ=255)
                                                                                                                                             ttgcccGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGc        <  3:1421214/149‑1 (MQ=255)
                                                                                                                                             ttgcccGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGc        >  3:366956/1‑149 (MQ=255)
                                                                                                                                             atgcccGACGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTgc                                                <  3:816915/108‑1 (MQ=255)
                                                                                                                                             atgcccGACGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTgc                                                >  4:816915/2‑109 (MQ=255)
                                                                                                                                                 ccGTCGGGCACGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAACCCAGGCACGCCGGt    >  2:1470931/1‑149 (MQ=255)
                                                                                                                                                   gTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCa  <  3:986170/149‑1 (MQ=255)
                                                                                                                                                   gTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCa  >  4:365292/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCA  >  NC_000913/1522568‑1522863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: