BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,693,416	A→G	5.2%	L283P (CTG→CCG) ‡	uidB ←	glucuronide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,693,416	0	A	G	5.2%	449.6 / 7.0	139	L283P (CTG→CCG) ‡	uidB	glucuronide transporter
Reads supporting (aligned to +/- strand): ref base A (68/63); new base G (3/5); total (71/68)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

ACCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGAT  >  NC_000913/1693268‑1693561

aCCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCa                                                                                                                                                   >  2:917533/1‑149 (MQ=255)

aCCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCa                                                                                                                                                   >  2:215664/1‑149 (MQ=255)

   aaTTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCg                                                                                                                                                <  2:1184494/149‑1 (MQ=255)

    aTTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCgg                                                                                                                                               >  2:692234/1‑149 (MQ=255)

    aTTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCgg                                                                                                                                               <  3:416580/149‑1 (MQ=255)

     ttGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGt                                                                                                                                              <  4:240432/149‑1 (MQ=255)

     ttGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGt                                                                                                                                              >  1:9396/1‑149 (MQ=255)

       gAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGc                                                                                                                                            <  1:324274/149‑1 (MQ=255)

          gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCgg                                                                                                                                         <  2:1487891/149‑2 (MQ=255)

          gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGa                                                                                                                                         <  4:1404260/149‑1 (MQ=255)

          gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGa                                                                                                                                         <  1:487293/149‑1 (MQ=255)

           cGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCggg                                                                                                                                        <  2:486623/149‑3 (MQ=255)

             aTGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGc                                                                                                                                      <  1:215664/149‑1 (MQ=255)

             aTGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGc                                                                                                                                      <  1:731442/149‑1 (MQ=255)

             aTGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGc                                                                                                                                      >  4:1228464/1‑149 (MQ=255)

               ggCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCca                                                                                                                                    <  4:754814/149‑1 (MQ=255)

               ggCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCca                                                                                                                                    >  4:1131227/1‑149 (MQ=255)

                  cAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCgggg                                                                                                                                       >  3:523182/1‑140 (MQ=255)

                  cAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCgggg                                                                                                                                       <  4:523182/143‑4 (MQ=255)

                  cAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAg                                                                                                                                 >  3:449424/1‑149 (MQ=255)

                  cAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACACAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAg                                                                                                                                 <  4:855932/149‑1 (MQ=255)

                   aaCGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGt                                                                                                                                >  2:289036/1‑149 (MQ=255)

                      gCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTacc                                                                                                                             >  4:663008/1‑149 (MQ=255)

                        aaccaacGCCACCGGCAGTTACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTaccaa                                                                                                                           >  3:1170709/1‑149 (MQ=255)

                        aaccaacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGc                                                                                                                                      <  3:258675/138‑1 (MQ=255)

                        aaccaacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGc                                                                                                                                      >  4:258675/1‑138 (MQ=255)

                        aaccaacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTaccaa                                                                                                                           >  3:1374758/1‑149 (MQ=255)

                         accaacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTaccaac                                                                                                                          <  1:958777/149‑1 (MQ=255)

                           caacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTaccaacca                                                                                                                        >  2:937601/1‑149 (MQ=255)

                            aacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAg                                                                                                                       <  1:1048680/149‑1 (MQ=255)

                                 cACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCTGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGTCACCAGCGGTGCCGATGCCACAGTACCCACCAGTtttt                                                                                                                  <  2:1451974/149‑1 (MQ=255)

                                 cACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGtttt                                                                                                                  >  4:1448453/1‑149 (MQ=255)

                                  aCCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTg                                                                                                                 <  2:1169392/149‑1 (MQ=255)

                                     ggCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAc                                                                                                              <  1:399438/149‑1 (MQ=255)

                                             ccAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCa                                                                                                      <  4:1168390/149‑1 (MQ=255)

                                               aGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGc                                                                                                    >  1:1251215/1‑149 (MQ=255)

                                                gACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCTAAGCGCCAATCATGAAGGCATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACCGTACCAACCAGGTTTTTCACCAGTACCAGca                                                                                                   >  3:1240304/1‑149 (MQ=255)

                                                gACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCa                                                                                                      >  2:357653/1‑146 (MQ=255)

                                                   ggAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAg                                                                                                >  2:1244559/1‑149 (MQ=255)

                                                        cccAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                                                           <  2:187980/149‑1 (MQ=255)

                                                         ccAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTac                                                                                                                              >  1:294475/1‑113 (MQ=255)

                                                         ccAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACa                                                                                          >  2:798973/1‑149 (MQ=255)

                                                         ccAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACa                                                                                          <  1:426163/149‑1 (MQ=255)

                                                           agaagaACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTa                                                                                                         >  1:190626/1‑132 (MQ=255)

                                                           agaagaACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGc                                                                                        >  4:1072091/1‑149 (MQ=255)

                                                             aagaaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGccc                                                                                      >  3:102291/1‑149 (MQ=255)

                                                               gaaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCgg                                                                                    >  4:97702/1‑149 (MQ=255)

                                                                aaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGt                                                                                   <  3:1448453/149‑1 (MQ=255)

                                                                aaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGt                                                                                   <  1:1427029/149‑1 (MQ=255)

                                                                   agcagATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATc                                                                                >  2:882886/1‑149 (MQ=255)

                                                                     cagATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAATAGCCCGGTATCAt                                                                              <  1:785601/149‑1 (MQ=255)

                                                                       gATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAttt                                                                            >  1:912888/1‑149 (MQ=255)

                                                                          aaCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAac                                                                         <  1:692234/149‑1 (MQ=255)

                                                                             cGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCa                                                                                                      >  1:405886/1‑117 (MQ=255)

                                                                             cGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCa                                                                                                      <  2:405886/117‑1 (MQ=255)

                                                                             cGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAAcaca                                                                      <  4:1258183/149‑1 (MQ=255)

                                                                               cAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATa                                                                    <  4:1045963/149‑1 (MQ=255)

                                                                                   ttCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGGACCAGCGGTGCCGATGCCACAGTACCAACCAGATTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgcgc                                                                >  4:1088019/1‑149 (MQ=255)

                                                                                    tCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTaa                                                                          >  2:429001/1‑138 (MQ=255)

                                                                                    tCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTaa                                                                          <  1:429001/138‑1 (MQ=255)

                                                                                      ccAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACg                                                             >  3:982637/1‑149 (MQ=255)

                                                                                       cAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGt                                                            >  1:443167/1‑149 (MQ=255)

                                                                                        aGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTa                                                           >  4:421785/1‑149 (MQ=255)

                                                                                        aGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTa                                                           <  1:1031331/149‑1 (MQ=255)

                                                                                         gCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAg                                                          <  3:836607/149‑1 (MQ=255)

                                                                                           aaaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaa                                                        <  3:764351/149‑1 (MQ=255)

                                                                                            aaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaac                                                       >  4:565866/1‑149 (MQ=255)

                                                                                              gcgcCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaa                                                     <  4:255030/149‑1 (MQ=255)

                                                                                                gcCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaac                                                    >  2:1229215/1‑148 (MQ=255)

                                                                                                gcCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaac                                                    <  1:1229215/148‑1 (MQ=255)

                                                                                                 ccAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacga                                                  >  3:1258106/1‑149 (MQ=255)

                                                                                                  cAATCAGTAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAAGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATTGCGCACGTAGAACgacgac                                                 <  4:927502/149‑1 (MQ=255)

                                                                                                     tCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAg                                              <  1:5353/149‑1 (MQ=255)

                                                                                                     tCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAg                                              <  4:1258106/149‑1 (MQ=255)

                                                                                                      cAGAAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAgg                                             <  2:1079882/149‑1 (MQ=255)

                                                                                                        ggAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGgcg                                           >  1:933692/1‑149 (MQ=255)

                                                                                                         gAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAc                                                                                                              >  1:301026/1‑81 (MQ=255)

                                                                                                         gAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAc                                                                                                              <  2:301026/81‑1 (MQ=255)

                                                                                                         gAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGgcgc                                          >  3:710815/1‑149 (MQ=255)

                                                                                                          aaGGTATTCTTTTTACCGATCCTCGCGCCCATCCCCGGCAACAGCGGTGCCGATGCCACTGCCCCAACAAGGTTTTGCACCAGTACCACCACAGTGAACAGCACGGTATCAATTAACACATAGCGCACGTAGAAAAACGACGAGGCGCt                                         <  4:1293846/149‑1 (MQ=255)

                                                                                                             gTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGAc                                      >  2:1140381/1‑149 (MQ=255)

                                                                                                                ttCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                                                              <  1:948920/106‑1 (MQ=255)

                                                                                                                ttCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATa                                                                    >  4:1220126/1‑116 (MQ=255)

                                                                                                                ttCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGc                                   <  3:337952/149‑1 (MQ=255)

                                                                                                                   tttttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAtt                                                                             <  3:1216594/104‑1 (MQ=255)

                                                                                                                   tttttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCaaa                                >  1:1307490/1‑149 (MQ=255)

                                                                                                                         ccGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGa                          >  1:805175/1‑149 (MQ=255)

                                                                                                                          cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGaa                         >  4:1206486/1‑149 (MQ=255)

                                                                                                                          cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGaa                         >  1:1026681/1‑149 (MQ=255)

                                                                                                                            aTCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAAt                       >  3:40753/1‑149 (MQ=255)

                                                                                                                               cTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAg                    >  1:428782/1‑149 (MQ=255)

                                                                                                                                tCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGc                   >  2:731075/1‑149 (MQ=255)

                                                                                                                                tCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGc                   >  4:366400/1‑149 (MQ=255)

                                                                                                                                         tCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAgc          <  3:1400224/149‑1 (MQ=255)

                                                                                                                                          catcGGCACCGCTGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGa                                       >  2:1190433/4‑119 (MQ=255)

                                                                                                                                          catcGGCACCGCTGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGa                                       <  1:1190433/116‑1 (MQ=255)

                                                                                                                                           atcGGCACCGCTGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAgcgc        >  2:96305/3‑149 (MQ=255)

                                                                                                                                            ccGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCa       >  3:479934/1‑149 (MQ=255)

                                                                                                                                                 aCCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGAt  <  2:805175/149‑1 (MQ=255)

                                                                                                                                                 aCCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGAt  <  3:1047031/149‑1 (MQ=255)

ACCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGAT  >  NC_000913/1693268‑1693561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`