Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,829,322 A→C 6.8% L138R (CTG→CGG)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,829,3220AC 6.8% 454.3 / 23.8 132L138R (CTG→CGG) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (71/52);  new base C (2/7);  total (73/59)
Fisher's exact test for biased strand distribution p-value = 7.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 135 total aligned reads displayed.

AGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCT  >  NC_000913/1829174‑1829469
                                                                                                                                                    |                                                                                                                                                   
agccTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCa                                                                                                                                                     <  2:472915/149‑1 (MQ=255)
agccTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCa                                                                                                                                                     >  4:841111/1‑149 (MQ=255)
agccTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCa                                                                                                                                                     >  3:804366/1‑149 (MQ=255)
 gccTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAg                                                                                                                                                    >  3:1379589/1‑149 (MQ=255)
         ccATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGt                                                                                                                                            >  3:345995/1‑149 (MQ=255)
          cATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACTAGGGAAATTATACGGCCCAAACACCGCCAGCACGTCGTg                                                                                                                                           >  4:925079/1‑149 (MQ=255)
              aaCGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCtgg                                                                                                                                       <  4:1269093/149‑1 (MQ=255)
              aaCGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCggg                                                                                                                                       <  4:284277/149‑1 (MQ=255)
               aCGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGc                                                                                                                                      >  2:281295/1‑149 (MQ=255)
               aCGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGc                                                                                                                                      >  2:1221003/1‑149 (MQ=255)
                 gCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGa                                                                                                                                    >  3:732726/1‑149 (MQ=255)
                  cATTACCGCTTCGCCACTCCACGGTGTCGGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCggt                                                                                                                                   <  4:251293/149‑3 (MQ=255)
                  cATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGAt                                                                                                                                   <  4:717167/149‑1 (MQ=255)
                   aTTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCggt                                                                                                                                   <  1:1252081/148‑3 (MQ=255)
                   aTTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCggt                                                                                                                                   >  2:1252081/1‑146 (MQ=255)
                   aTTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATg                                                                                                                                  >  1:1420856/1‑149 (MQ=255)
                     tACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCGgtgtt                                                                                                                                <  2:529999/149‑2 (MQ=255)
                      aCCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCGgtgttt                                                                                                                               <  4:358410/149‑3 (MQ=255)
                      aCCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCg                                                                                                                               >  2:1370365/1‑149 (MQ=255)
                        cGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCa                                                                                                                             >  1:50891/1‑149 (MQ=255)
                           ttCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGc                                                                                                                          >  2:345250/1‑149 (MQ=255)
                               ccACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgc                                                                                                                      >  2:144790/1‑149 (MQ=255)
                                cACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgcc                                                                                                                     >  3:600973/1‑149 (MQ=255)
                                  cTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGa                                                                                                                                    <  2:542195/132‑1 (MQ=255)
                                  cTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGa                                                                                                                                    >  1:542195/1‑132 (MQ=255)
                                   tCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgcg                                                                                                                  >  1:1280719/1‑149 (MQ=255)
                                     cACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgcgcc                                                                                                                >  1:1438508/1‑149 (MQ=255)
                                              aGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCAt                                                                                                       <  2:1420856/149‑1 (MQ=255)
                                              aGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCAt                                                                                                       >  1:296541/1‑149 (MQ=255)
                                               gTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAgg                                                                                                                           <  2:1261952/128‑1 (MQ=255)
                                               gTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAgg                                                                                                                           >  1:1261952/1‑128 (MQ=255)
                                               gTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCAtt                                                                                                      <  4:229373/149‑1 (MQ=255)
                                                   gCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTc                                                                                                             >  4:278614/1‑138 (MQ=255)
                                                    cTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACt                                                                                                 >  2:562283/1‑149 (MQ=255)
                                                     tGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTa                                                                                                >  2:148438/1‑149 (MQ=255)
                                                        gTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgc                                                                                             <  3:1261353/149‑1 (MQ=255)
                                                            aaaGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgctgct                                                                                         <  4:600973/149‑1 (MQ=255)
                                                             aaGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTc                                                                                        >  4:709723/1‑149 (MQ=255)
                                                               gATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGc                                                                                      <  4:88400/149‑1 (MQ=255)
                                                                           aCCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCAtt                                                                                                      >  2:208481/1‑121 (MQ=255)
                                                                           aCCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCAtt                                                                                                      <  1:208481/121‑1 (MQ=255)
                                                                           aCCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTa                                                                                                <  4:752638/127‑1 (MQ=255)
                                                                           aCCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTa                                                                                                >  3:752638/1‑127 (MQ=255)
                                                                           aCCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGt                                                                          >  2:391471/1‑149 (MQ=255)
                                                                            ccTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTg                                                                         <  4:345995/149‑1 (MQ=255)
                                                                               gCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATa                                                                      >  3:274284/1‑149 (MQ=255)
                                                                               gCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATa                                                                      <  2:296541/149‑1 (MQ=255)
                                                                                ccAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCg                                                                                    >  1:912060/1‑134 (MQ=255)
                                                                                     aaTGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCATGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCtt                                                                >  3:1027963/1‑149 (MQ=255)
                                                                                     aaTGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCtt                                                                >  3:895531/1‑149 (MQ=255)
                                                                                      aTGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTa                                                               >  4:580041/1‑149 (MQ=255)
                                                                                      aTGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTa                                                               <  1:1221003/149‑1 (MQ=255)
                                                                                       tGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTTGCCGGTACGAACGTGATACGCCTTaa                                                              <  2:1011709/149‑1 (MQ=255)
                                                                                         ccGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCAAGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAAtt                                                            >  1:300220/1‑149 (MQ=255)
                                                                                          cGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACATCGCCCGCACGGCGTGCTGGCGgtgtttgg                                                                                                                             >  1:331514/1‑79 (MQ=21)
                                                                                           ggAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGa                                                          >  1:694352/1‑149 (MQ=255)
                                                                                           ggAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGa                                                          >  1:841996/1‑149 (MQ=255)
                                                                                           ggAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGa                                                          >  3:505228/1‑149 (MQ=255)
                                                                                            gAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTCTACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTAGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGat                                                         <  2:724650/149‑1 (MQ=255)
                                                                                            gAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGat                                                         <  3:319175/149‑1 (MQ=255)
                                                                                            gAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGat                                                         <  3:1122834/149‑1 (MQ=255)
                                                                                                gATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATcg                                                     >  1:483005/1‑149 (MQ=255)
                                                                                                 atatGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTc                                                                                                             >  3:1208948/1‑92 (MQ=255)
                                                                                                 atatGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTc                                                                                                             <  4:1208948/92‑1 (MQ=255)
                                                                                                 atatGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATcgc                                                    >  1:1497321/1‑149 (MQ=255)
                                                                                                 atatGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATcgc                                                    >  1:391573/1‑149 (MQ=255)
                                                                                                  tatGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATcgcg                                                   >  4:645094/1‑149 (MQ=255)
                                                                                                    tGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGTCTTAATTGATATCGCGAt                                                 <  4:867468/149‑1 (MQ=255)
                                                                                                      tCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGAttt                                               >  3:301544/1‑149 (MQ=255)
                                                                                                      tCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGAttt                                               >  2:85055/1‑149 (MQ=255)
                                                                                                        cGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCATGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTa                                             <  4:133599/149‑1 (MQ=255)
                                                                                                         gTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAt                                            <  3:1323743/149‑1 (MQ=255)
                                                                                                          ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                           <  1:112856/149‑1 (MQ=255)
                                                                                                          ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                           <  1:1422847/149‑1 (MQ=255)
                                                                                                          ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                           <  4:1027963/149‑1 (MQ=255)
                                                                                                          ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                           <  3:1022657/149‑1 (MQ=255)
                                                                                                            cGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGa                                         <  4:651354/149‑1 (MQ=255)
                                                                                                             ggCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGat                                        <  3:992390/149‑1 (MQ=255)
                                                                                                                aaaTGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAAc                                                                            <  1:584746/110‑1 (MQ=255)
                                                                                                                        aGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCa                             >  4:1211376/1‑149 (MQ=255)
                                                                                                                        aGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCa                             >  2:563199/1‑149 (MQ=255)
                                                                                                                        aGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCa                             >  3:1249889/1‑149 (MQ=255)
                                                                                                                          ggAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACt                           >  3:742544/1‑149 (MQ=255)
                                                                                                                          ggAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACACCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACt                           >  2:482834/1‑149 (MQ=255)
                                                                                                                                  taCGGCCCTAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGc                   <  2:300220/149‑1 (MQ=255)
                                                                                                                                  taCGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGc                   >  3:1329653/1‑149 (MQ=255)
                                                                                                                                  taCGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGc                   <  1:682935/149‑1 (MQ=255)
                                                                                                                                      gCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATa                                                                      >  3:242577/1‑94 (MQ=255)
                                                                                                                                      gCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATa                                                                      <  4:242577/94‑1 (MQ=255)
                                                                                                                                      gCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCt               >  1:408977/1‑149 (MQ=255)
                                                                                                                                        ccAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTc             >  2:1308512/1‑149 (MQ=255)
                                                                                                                                        ccAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTc             >  4:1122681/1‑149 (MQ=255)
                                                                                                                                             cacCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAgc        >  1:1190584/1‑149 (MQ=255)
                                                                                                                                             cacCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAgc        <  2:841996/149‑1 (MQ=255)
                                                                                                                                              acCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAgcg       >  4:989755/1‑149 (MQ=255)
                                                                                                                                                cGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGcgcg     >  1:241334/1‑149 (MQ=255)
                                                                                                                                                 gCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCgg    >  4:1088251/1‑149 (MQ=255)
                                                                                                                                                  ccAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGc   <  4:1379589/149‑1 (MQ=255)
                                                                                                                                                  ccAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGAATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGc   <  4:178041/149‑1 (MQ=255)
                                                                                                                                                   cAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCt  <  3:580041/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCT  >  NC_000913/1829174‑1829469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: