Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC21.3% 86.2 / 10.6 48intergenic (+474/+168)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (17/20);  new base C (8/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 7.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATA  >  NC_000913/4296007‑4296538
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                    
tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAAGAGCCTGATTTACCGTGATTCTGTAGGACG‑GATCACGCGTTTACGCCGCATCCGACa                                                                                                                                                                                                                                                                         >  3:711533/1‑275 (MQ=255)
     cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCTGCCGCCTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACTCCGAAC‑AGCCCATTAACCTGCTATCGCTTGATTTTTTAGTTCG‑GATAATGCGTTTACGCCGCCTCCGACATCa                                                                                                                                                                                                                                                                      >  4:597789/1‑273 (MQ=11)
         aTTTTGTAGGTCGGATAAGGCGTTTACGCTGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTCAGCGTGATTTTGTAGGTCG‑GATAAGGCGCTTACGCCGCATCCGACATCAATGcc                                                                                                                                                                                                                                                                 >  2:598487/1‑274 (MQ=255)
         aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGCTTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATACGGCGTTTATGCCGCCTCCGCCATCAATGcc                                                                                                                                                                                                                                                                 >  2:74139/1‑274 (MQ=255)
         aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTCTCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCG‑GATCCGGCGTTTACTCCGCATCCGCGATCaatgac                                                                                                                                                                                                                                                                 >  4:522594/1‑270 (MQ=11)
                       aTAAGGCGTTTCCGCCGCCTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCttgc                                                                                                                                                                                                                                                  <  1:817699/275‑3 (MQ=19)
                                      cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                   >  1:72620/1‑275 (MQ=14)
                                         aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa                                                                                                                                                                                                                                >  1:721543/1‑275 (MQ=14)
                                              aCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGGGATTTTGTAGGTCG‑GATAAGGCGTTTACGCCGCATCCGCCATCAATGCTTGATGCGACGCTTCCCGCGTCTAATcctgtctcttag                                                                                                                                                                                                                            >  2:506166/1‑263 (MQ=11)
                                                                                                                                             gCGTTTATGCCGCATCCGACATCAACGCCTGATGCTACGCTTAACGCGTCTTATCAGGCCGACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                        <  2:316419/275‑1 (MQ=255)
                                                                                                                                                              gctataagcGCCTGATGCTACGCTCAACGGGTCGTATCGGGCCTA‑GTCCGACAACCGCAAGGGCCTGAGTTAGGGTGCTGTGGTGGTGCG‑GTTAAGGCTGTTATGCGGCTTCCGACATCAACGCCTGATGAGACGCTTAACGCGTTTGATCAGGCCTACGCCAGACAGCGCAAGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCGGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCt                                                                                                        <  3:50446/266‑1 (MQ=2)
                                                                                                                                                                           gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCg                                                                                                                                                                                                                                                                            >  1:555541/1‑101 (MQ=32)
                                                                                                                                                                            aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGttca                                                                                          >  1:788447/1‑272 (MQ=2)
                                                                                                                                                                                   gcttgccgcgTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTATCTTAACCGGTGGTTCATTTTTCa                                                                                   >  2:230886/7‑274 (MQ=255)
                                                                                                                                                                                    cttgccgagTCTTATCAGGCCTAAGCCAGAC‑AACGAAATAGCCTGATTTAGCTTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                 <  1:55313/267‑1 (MQ=11)
                                                                                                                                                                                                tATCAGGCCTACGCCAGAC‑AGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCACCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                      <  1:25150/274‑1 (MQ=255)
                                                                                                                                                                                                tATCAGGCCTACGCCAGAA‑ACGGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                      <  1:415913/274‑1 (MQ=255)
                                                                                                                                                                                                gaTCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                      <  2:167386/273‑1 (MQ=255)
                                                                                                                                                                                                      gCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTacc                                                               >  1:295816/1‑273 (MQ=255)
                                                                                                                                                                                                         tACGCAAGAA‑AGCGCAATGGCCTGATTTAGCGTGAGTGTGTAGGTCG‑AATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                            <  1:93588/275‑1 (MQ=14)
                                                                                                                                                                                                          aCGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                            <  2:358373/274‑1 (MQ=255)
                                                                                                                                                                                                          aCGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                            <  2:597789/274‑1 (MQ=255)
                                                                                                                                                                                                           cGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc                                                          >  1:344732/1‑275 (MQ=255)
                                                                                                                                                                                                           cGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAACGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATCTTGTCGGTCGGATACGGCGTTT‑‑ACGC‑‑CGCATCCGACATAATTGCCTGATGCGACGTTTGCCGCCTCTTCTACAGCCTATCCTACCTGTTGGTTCATTTTTCTAACTCTTTACACCCGCTATccatc                                                           >  3:411956/1‑270 (MQ=11)
                                                                                                                                                                                                              gaGAC‑AGCGCAATAGCCTGTTTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCTGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                                                       <  2:670925/274‑1 (MQ=255)
                                                                                                                                                                                                              cAGAC‑AGCACATTAGCCTGATTTAGCGTTATTTGGTGGGTCG‑GCTACGGCGTTGATGCCGCAGCCTACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                                                       <  3:550467/275‑1 (MQ=17)
                                                                                                                                                                                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGTCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTT‑‑ACGC‑‑CCCATCCGACATACATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCGTTGGCTAATCTTTTAAATTCTCCCCACCACGTATCAAACCCtccaacaca                                                >  3:615413/1‑267 (MQ=11)
                                                                                                                                                                                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCACTGCCTGATGCTCCCCTTTCCGCGTCTTATCCGGCCTATCTTAACCGTTGGTTACTTTTTCCAACTCTTCACCCCCTGTATCACACCTTTctctta                                                 >  1:453797/1‑269 (MQ=14)
                                                                                                                                                                                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                                                >  2:420540/1‑275 (MQ=255)
                                                                                                                                                                                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CCCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACCCTTCACACCCGGTACCAAACCCTtccatcca                                                >  3:578341/1‑275 (MQ=255)
                                                                                                                                                                                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGAGACGATTGCCGCGTCTCATCAGTCCTATCTAAACCGTTGGTTACTTTTTCACAATCTTCACACCCGGTTTCAAACCCTTCCAtctg                                                >  4:691825/1‑272 (MQ=14)
                                                                                                                                                                                                                         aaTAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                             <  2:294775/275‑1 (MQ=255)
                                                                                                                                                                                                                              acttgtttAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                        <  1:89586/270‑1 (MQ=255)
                                                                                                                                                                                                                                tGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGtt                                       >  1:825493/1‑274 (MQ=255)
                                                                                                                                                                                                                                 gATTTGGCTGGAGTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCACCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                        <  2:542888/272‑1 (MQ=255)
                                                                                                                                                                                                                                 gATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                        <  2:239768/272‑1 (MQ=255)
                                                                                                                                                                                                                                   tttAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                 >  3:463537/1‑261 (MQ=255)
                                                                                                                                                                                                                                     taccggtaTTTTGGAGGTCG‑GATAAGGCGTTTAGGCCGCATCCGACATCCACGCCTTATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTc                                      <  4:592140/263‑1 (MQ=17)
                                                                                                                                                                                                                                       gCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                             <  1:463537/261‑1 (MQ=255)
                                                                                                                                                                                                                                        cGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTTCATACAGCTCCGGTTCAACCattc                              >  4:454801/1‑272 (MQ=255)
                                                                                                                                                                                                                                        agTGATTGCGTAGGTCGTGAT‑AGGCATTTATTCACCATCCGACAGTAAAGCCTAAGGCAACGCGTACCGCTTCTTTTCATGCCTAGGCCAGGCAGCACAGTAGCCGGATTGTGCGTGATTTTGTAGGTCGGATCGTTCCTTTACA‑‑C‑‑CTCAGCCGACATCAAGGCCTGATTCGACGCTTGCCGCGTCTTATCAGGCCTATCTTTACCGTGGGTTAATTTTTCGAACTCATCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTg                              <  4:484036/274‑1 (MQ=255)
                                                                                                                                                                                                                                           gATTTTGTAGGTCG‑GATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGc                             <  3:825493/273‑1 (MQ=255)
                                                                                                                                                                                                                                            aTTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCCTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCTGGTATCAAACCCTTCCATACAGCTCCGGTTCAATCAGCGCat                           >  2:349886/1‑273 (MQ=255)
                                                                                                                                                                                                                                                       cG‑GATAAGGGGTTTATGCCCCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CGACGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGc                 <  1:801139/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATCCAGCTCAGTTTCAACCCTTGCAGCGCCTTTTGCTTGttcttcc       >  2:248719/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTtcttct       <  1:663375/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                 agTTTATGCCGCATCCTACATAAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAATGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTtcttct       <  2:691825/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCCCCTTTTGCTTGTTCTTCTCGATa  >  2:315254/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                    
TTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAC‑AGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCG‑GATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATA  >  NC_000913/4296007‑4296538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑