Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,535,964 A→T E44V (GAA→GTA)  crr → glucose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,535,9640AT97.7% 534.2 / NA 171E44V (GAA→GTA) crrglucose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (74/93);  minor base G (2/2);  total (76/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 175 total aligned reads displayed.

GAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTC  >  NC_000913/2535692‑2536234
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                              
gaagagggaagggtggtAACAGAGCAGGTTTTTGCTAAAACGAAAAAGGACGATGTAAGGAAGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGtaa                                                                                                                                                                                                                                                                              <  4:355118/264‑1 (MQ=255)
 aaGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGATCCGCTCTCTGGCGAGACCGTCAATATTAAAGACGTGCCGGAGGTCGTTTTTTCGGtaaa                                                                                                                                                                                                                                                                             >  4:597069/1‑275 (MQ=255)
      gctaaGGTGTTAGCAGATCAGGTTCTTGCTCAACCGACAACGGACGAGTTAATTCCTCTGGTTAACAAGTTCATTGAAGAAAAAACGATCTGCTAATCCACGAGATGCGGCCCAATTTCCTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCg                                                                                                                                                                                                                                                                        <  2:252239/272‑1 (MQ=255)
      gCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCg                                                                                                                                                                                                                                                                        <  1:478042/275‑1 (MQ=255)
       cGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTCTTGAGCTCATTGCTCCGCTCTCTGGCGAGATCGGCAATATCGAAAACGTGCCGGATGTGTTTTTTGCGGTAAAAATTGt                                                                                                                                                                                                                                                                       >  3:139119/1‑275 (MQ=255)
             tgtTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGa                                                                                                                                                                                                                                                                 <  1:607300/275‑1 (MQ=255)
              gtTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGACGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATGTCGACGCCGTGCCGGATGTCGTTTTGGCGGTAAAAATCGTTGGTGAt                                                                                                                                                                                                                                                                >  3:365647/1‑275 (MQ=255)
                   caggacatgCTCGTGCTCAACCGAGAACGGACGAGTTAATGACGCTGGTTAAAAAGTTCATTGAAGAAAAAACAATCTGCGAATCGACGAGATGCGGCCCAATGTACTGCTTAGGTGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTAt                                                                                                                                                                                                                                                           <  4:296022/267‑1 (MQ=255)
                   aagggcaaggtgttGCTCAAACGACAAAGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAAGTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTAt                                                                                                                                                                                                                                                           <  4:181063/263‑1 (MQ=255)
                   aagagcagGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAAAAAGTTCATTGAAGAAAAAACAATCTGCTAATCCCCGGAATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTAt                                                                                                                                                                                                                                                           <  1:754906/274‑1 (MQ=255)
                    agagcagGGTCTTGCTAAACAGACAACGGACGAGTTAATGGCGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAAGCCACGAGATGCCGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTAt                                                                                                                                                                                                                                                           <  3:124689/274‑1 (MQ=255)
                       gcagGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGTCGGAGGTCGTTTTTGCGGTCAAAATCGTTGGTTATGGTATTGCt                                                                                                                                                                                                                                                       >  1:118989/1‑275 (MQ=255)
                       gcagGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTCAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGATCTCTGGCGAGACCGTAAATATCGAAGACGTGCCGGATGTCGTTTTTTGGGTAAAAATCGTTGTGGATGGTATTGCt                                                                                                                                                                                                                                                       >  4:216882/1‑275 (MQ=255)
                                       ccGACAACGGACGAGTTAATGACGCTGGTTAAAAAGTTCATTGAAGAAAAAACAATCTGCTAATCCCCGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGt                                                                                                                                                                                                                                        <  1:770283/274‑1 (MQ=255)
                                            aaCGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAAGTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACaaa                                                                                                                                                                                                                                  <  2:145118/275‑1 (MQ=255)
                                                 acaaGTTAATGACGCTTATTAACAAGTTCTTTGAAAAAAAAACAATCTGCTAATCCCCGTGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGTGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCAGTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGt                                                                                                                                                                                                                             <  4:151901/272‑1 (MQ=255)
                                                 aCGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGCCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGt                                                                                                                                                                                                                             >  1:721674/1‑275 (MQ=255)
                                                   gAGTTAATGGCGGTGGATAACAAGTTCATTTAAGAAAAAACAGTCTGATAATACACGAGATGCGGACCAATGTACTGCTTAGGAAAAGATCATGGGTTTGTTCAATAAACTGAAGTCGCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTAAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTcg                                                                                                                                                                                                                           <  2:384554/275‑1 (MQ=255)
                                                   gAGTGAATGAGACTGGTTAACAAGTTCAGTGAATAAAAAACCATCTGCGACTGCCCGGGATGCGGCACAATTTACGGCTTAGGAGAAGATCATGGGTGTGTGCGATAAACTGAAATCTCTGGTTGCCGAGGACAAGAAGGATACCGGAACTATTGAGATCGTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGGcg                                                                                                                                                                                                                           <  3:536433/275‑1 (MQ=255)
                                                      ttAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGGGATGCGGACCAATTTACTGATTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCgcgc                                                                                                                                                                                                                        <  1:55089/275‑1 (MQ=255)
                                                        aaTGACGCTGGTTAACAAGTTCATTTAAGAAAAAACAATCTGCTAATCCCCGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCa                                                                                                                                                                                                                      <  2:2452/275‑1 (MQ=255)
                                                           ggcagtGGTTAACAAGTGCATGTATGAAAAAAGAATCGGCTAATCCACGGGATGCGGCCCAATTTACGGCTTAGGCACAGATCATGGGTTTGGTCGATAAACTGAAAACTCTGGTTTCCGACGAAAAGAAGGATACCGGAACTATTGAGATCATTGCTGCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTa                                                                                                                                                                                                                   <  3:269718/270‑1 (MQ=255)
                                                               cTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTTCTCCGCTCTCTGGCGACATCGTCAATATCGAAGACGTGCCGGATGTAGTTTTTGCGGTAACAATCGTTGGTGTGGGTATTGCTATCAAATCAACGGTGTACCAAATGGTCGCGCACGTGGACg                                                                                                                                                                                                               >  4:103874/1‑275 (MQ=255)
                                                               cTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGGCCCAGTAGACg                                                                                                                                                                                                               >  1:344971/1‑275 (MQ=255)
                                                               cTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGGAAAAATCGTTGGTGATGGTATTGCTTTCAAACCAACGGGTAACAAAATGGCTGCGCCAGtgg                                                                                                                                                                                                                  >  3:486540/1‑270 (MQ=255)
                                                                     aaaaaGTTCATTGAAGAAAAAACAAACTGCTGATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCa                                                                                                                                                                                                         <  4:120348/272‑1 (MQ=255)
                                                                            tCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGTCGAGCTCGCCAATATCGAAGACGTGCCGGTTGTCGTTTTTGCGGTAAAAATCGTTCGTGATGGTATTGCTATCAAACAAACTGTTAACAAAATGGTCGCTCCAGTAGGCCGCCCCATTGCTaa                                                                                                                                                                                                  >  4:418872/1‑275 (MQ=255)
                                                                             cATTGAAGAAAAAAGATTCTTCTAATCCACGAGATGCGGCCCAATTTTCTGGTTAGGAGAAGATCATGGGTTTGTTCGTGAAACTGAAATCTCTGGTTTCCGACGACAAGTAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATAGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTaaa                                                                                                                                                                                                 <  4:249554/275‑1 (MQ=255)
                                                                                tGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCg                                                                                                                                                                                                                                                                        <  4:183860/201‑1 (MQ=255)
                                                                                          aCAATCTGCGAATCCACGCGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCtgaaacca                                                                                                                                                                                      <  4:75649/273‑8 (MQ=255)
                                                                                              tCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAACAGGGTCGCGCCAGTAGACGGCACCATTGGACAATTCTTTGAaacaaacc                                                                                                                                                                                 >  2:208020/1‑274 (MQ=255)
                                                                                                acctatcccACGGAATACGGCCCAATTTACTTCTTAGGAGAAGATCATAGGTTGGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACg                                                                                                                                                                              <  4:538541/268‑1 (MQ=255)
                                                                                                   tAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTTAGATCATTGCTCCGCTCTCTGGCGAGATCGACAATATCGAAGACGTGCCGGATGCGGTTTTTGCGGTAACAATCGTTGGTGATGGTATTCCTATTAAACCAACGGGTAACAAAAGGGTCGGCGCTGTAAGCGGACCCCTTGGGAAAATCCTTTAaaccaacccag                                                                                                                                                                              >  4:632144/1‑269 (MQ=255)
                                                                                                         caggtaggCGGCTTAATTGACTGCGTAGGAGATGATCATGGGGTTGTTAGATAAACTGAAATCTCTGGTTGCCGACGACAAGAAGGATACCGGAACTAGTGAGATCATGGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAAACAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTa                                                                                                                                                                     <  2:685214/268‑1 (MQ=255)
                                                                                                          cGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGTGACAGTCAACGGCACCATTGGTAAAATCCTTGAAACCAACCACGCATTCTCTAt                                                                                                                                                                    >  3:404721/1‑275 (MQ=255)
                                                                                                               tGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATAACGAAGACGTGCCGGATGTCGTTTGTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCTCACCAGTAGACGGCACCAGTGGGAAAATCTTTGAAACCAACCACGCAGTCCCTACCGAAt                                                                                                                                                               >  3:803373/1‑275 (MQ=255)
                                                                                                                gCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGAGCGTCAATATCGGAGAACACGCGGATGTCGTTTTTGCGGTCAACATCGTTGGTGCTGGGCGTGCCAGCAAACCTATGGGGAACAAAGAGGTCGTGACAACAGACGGTCACATTGGGAACAGTTTTTAACATAACCACGCAtacataaaccaaat                                                                                                                                                              >  4:693879/1‑262 (MQ=255)
                                                                                                                      tcaTTTACGGCTTAGGAGAAGAGCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGGAGGATACCGGAACGATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAACTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACCAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg                                                                                                                                                        <  1:157362/273‑1 (MQ=255)
                                                                                                                      gaaTTTAATGGTTAGGGGAAGATCATGGGTTTGTTCCATAAACTGAAATATCTGGTTTCCAACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGGGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGGGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg                                                                                                                                                        <  3:721674/274‑1 (MQ=255)
                                                                                                                      cAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg                                                                                                                                                        <  3:771272/275‑1 (MQ=255)
                                                                                                                         tttACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCTGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAACCGGCACCATTTGTAAAATCTTTGAAACCAAGCACGCATTCTCTAACGAATCTTATAgagg                                                                                                                                                     >  1:731383/1‑275 (MQ=255)
                                                                                                                                cTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCTGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGa                                                                                                                                               <  1:519371/274‑1 (MQ=255)
                                                                                                                                  tagggaaagaTCATGGGTTTGTTCGATAAACTGAAATCGCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAAc                                                                                                                                             <  3:523776/268‑1 (MQ=255)
                                                                                                                                   aGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAAAAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCCTTCTCTATCGAATTTGGTAGCGGCGTTGAACTg                                                                                                                                           >  1:96603/1‑275 (MQ=255)
                                                                                                                                   aGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGCTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTg                                                                                                                                           >  1:493706/1‑275 (MQ=255)
                                                                                                                                    ggAGAAGATCATGGGTTTGTTGGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGt                                                                                                                                          <  2:173114/275‑1 (MQ=255)
                                                                                                                                          gagcaagggTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTATCTGGCGAGATCGTCAATAGCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCa                                                                                                                                   <  2:328780/269‑1 (MQ=255)
                                                                                                                                          gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTg                                                                                                                                                                                                                                                         >  1:243059/1‑158 (MQ=255)
                                                                                                                                          gaTCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCCAATCTGATAGCGGCGTTGAACTGTTCGTc                                                                                                                                     >  1:794738/1‑274 (MQ=255)
                                                                                                                                           ttCATGGGTTGTTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGCTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCa                                                                                                                                   <  3:493706/274‑1 (MQ=255)
                                                                                                                                             aagaggTTTGTTCGATAAAATGAAATCCCTGGTTTCCGACGCCAAGAAGGATACCGGAACTATGGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACt                                                                                                                                 <  3:152805/271‑1 (MQ=255)
                                                                                                                                              aTGGGTTTGTTCGATCAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGAAACTATTGAGACCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCCTTTTTGCGGTACAAATCGCTGGTGATg                                                                                                                                                                                                                                                               >  3:712097/1‑148 (MQ=255)
                                                                                                                                              aTGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTAt                                                                                                                                                                                                                                                     <  3:243059/158‑1 (MQ=255)
                                                                                                                                               tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCAAGTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTTCCTATCGAATCTGATAGCGGCGTTACATTTTTCGTCCCCTTc                                                                                                                               >  3:23534/1‑275 (MQ=255)
                                                                                                                                               tGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCGCTGGCGAGATCGTCAATATCGAAGACGTGCCGGAAGTCGTTTTTGCGGTAACAATCGTTGGTGTTGGTATTGCTGTCAAACCCACGGGTAACAAAATGGTCGCGCCAGTAGACGGGACCATTGGAAAAATCTTTGAAACCAAACCCGCATTCTCTATCGAATCTGATCGAGGCGGTGAAGTGGTTGACCACtcc                                                                                                                               >  3:109623/1‑273 (MQ=255)
                                                                                                                                                     tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATc                                                                                                                         >  2:124137/1‑275 (MQ=255)
                                                                                                                                                       ttCGATAAACTGAAATCTCTTGTTTCCGACGACAAGAAGGATACCTGAACTATTGAGATCCTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAATACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa                                                                                                                       <  1:132485/275‑1 (MQ=255)
                                                                                                                                                       ttCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTTCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTTATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa                                                                                                                       <  2:76126/275‑1 (MQ=255)
                                                                                                                                                       gtCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGa                                                                                                                       <  1:282142/274‑1 (MQ=255)
                                                                                                                                                          gaaaaaCTAAAATCTCTGGTTTCCGGCGACAAGAAGGATACCTGAACTATTGGCATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGaca                                                                                                                     <  3:214788/271‑1 (MQ=255)
                                                                                                                                                            tAAACTGAAATCTCGGGTTTCCGCCGACAAGAAGGAGACCGGAACCAGTGCGATCATTGCGCGTCTCTCTGTCGAGATCGTCAATAGCGAAGACGGGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCg                                                                                                                  <  4:274238/275‑1 (MQ=255)
                                                                                                                                                                           tggttCCCACCACAAAAAGGATACCGGAACTTTTTAGGTCCTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAAACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCg                                                                                                   <  3:705479/272‑1 (MQ=255)
                                                                                                                                                                           ggTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCg                                                                                                   <  3:28272/275‑1 (MQ=255)
                                                                                                                                                                                   acgacAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTc                                                                                           <  2:241920/275‑1 (MQ=255)
                                                                                                                                                                                        aagaagGATACCGGAACGAGTGAGATCATTGCTCCGGTCTCTAGCGGGATAGTCAATATCGAAGACGTGCCAGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCg                                                                                      <  3:431420/275‑1 (MQ=255)
                                                                                                                                                                                                 aCCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTTCTga                                                                             >  1:381068/1‑275 (MQ=255)
                                                                                                                                                                                                 aCCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTg                                                                              <  3:757711/274‑1 (MQ=255)
                                                                                                                                                                                                     gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGaa                                                                                                                                              <  2:56160/206‑1 (MQ=255)
                                                                                                                                                                                                     gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTTGGTATCGACACAGTGGAACTGAAAGGCGAAGGCTTCAGGCGTATTGCTgaagaa                                                                         >  2:270376/1‑275 (MQ=255)
                                                                                                                                                                                                        cTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGt                                                                      >  2:598798/1‑275 (MQ=255)
                                                                                                                                                                                                          atttggaTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCa                                                                    <  1:515438/270‑1 (MQ=255)
                                                                                                                                                                                                          aTTGAGATCATTGCTCCGGTCTATGGCGAGATCATCAATATTGAAGACGTTCGGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGTTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCa                                                                    <  1:511227/275‑1 (MQ=255)
                                                                                                                                                                                                                aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTg                                                                                                                                                            >  2:36560/1‑181 (MQ=255)
                                                                                                                                                                                                                aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGt                                                              >  1:319388/1‑275 (MQ=255)
                                                                                                                                                                                                                aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTCGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGt                                                              >  3:326180/1‑275 (MQ=255)
                                                                                                                                                                                                                   aTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTCAAGAAGGTCAGcgcg                                                               >  1:427445/1‑271 (MQ=255)
                                                                                                                                                                                                                    ttGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAg                                                                                                                                                        <  4:36560/181‑1 (MQ=255)
                                                                                                                                                                                                                     tGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGaaa                                                          <  1:747073/274‑1 (MQ=255)
                                                                                                                                                                                                                     tGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAg                                                         >  1:424087/1‑275 (MQ=255)
                                                                                                                                                                                                                     tGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAg                                                         >  1:107892/1‑275 (MQ=255)
                                                                                                                                                                                                                         ccGCTCTCTGGCGAGATCGTCAATATCGAAGACGTTCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTgg                                                     <  1:23534/275‑1 (MQ=255)
                                                                                                                                                                                                                            ctctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTTGCGCCAGTAGACGGCACCATTGGTAAAATCttt                                                                                                                                                                                           >  1:209277/1‑138 (MQ=255)
                                                                                                                                                                                                                            ctctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACATCGTTGAACTGAAAGGCGACGGCTTCAAGCGTATTGCTGATGAAGGTCACAGCGTGAAAGTTGGCGa                                                  >  3:446680/1‑275 (MQ=255)
                                                                                                                                                                                                                            ctctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGa                                                  >  2:502870/1‑275 (MQ=255)
                                                                                                                                                                                                                            ctctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCACCGCGCGAAAGTTGGCgg                                                  >  1:710805/1‑274 (MQ=255)
                                                                                                                                                                                                                             tctctGGGGGTATCGTAAAAATGGAAGGAGAGGCGGATGACGTTTCTGCCGTAAAAAGCTTAGGTGATGGTATTGCGATCAAACCAACGGGTAACAAAAGGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCGCTGTCGAATCTGATAGCAGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTAAAGAAGGTCAGCGCGTGAAAGTTGGCGa                                                  <  4:315384/274‑1 (MQ=255)
                                                                                                                                                                                                                             tatctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGa                                                  <  2:586586/272‑1 (MQ=255)
                                                                                                                                                                                                                              ctctGGCGAAATGGGCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGGGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATa                                                <  1:90455/275‑1 (MQ=255)
                                                                                                                                                                                                                               tctGGGGAGATCGTCAATATCCAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATa                                                <  1:147677/274‑1 (MQ=255)
                                                                                                                                                                                                                                    cGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCa                                          <  1:446680/275‑1 (MQ=255)
                                                                                                                                                                                                                                      agaTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGCGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGACAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCAATTCGGTATCGACACCGTTTAACTGAAAGGCGAAGGCTCCAAGCGTATTGCTGAAGAACGTCAGCGCGTGAAAGTTGGCGATCCCGTCAt                                         >  3:130409/1‑274 (MQ=255)
                                                                                                                                                                                                                                                           tGCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGAGGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGAGAAGGCTTCAAGCGTATTGCTGAAGTAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCTctgctg                   >  2:275418/1‑275 (MQ=255)
                                                                                                                                                                                                                                                            gCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATg                                                                                                                                                                                                                                                               >  1:57823/1‑38 (MQ=255)
                                                                                                                                                                                                                                                            gCCGGATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTTAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTgg                  >  2:216984/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                gATGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTAt                                                                                                                                                                                                                                                           <  3:57823/38‑1 (MQ=255)
                                                                                                                                                                                                                                                                 aTGTCGTTTTTGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAagag             <  1:130401/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tttttGCTATAAAACGCGGTGGTTAGGGTCATGCTAACAAACCGACGGGTAACCACCTGGACACACCAGTAGACTGCACCATTGGTAAAATCTTAGAAACCAACCACGCATTCTCGATCGAAGCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGAAACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc        <  4:267205/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tttttGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc        <  2:181231/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tttttGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc        <  1:37864/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tttttGCGGTAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATAGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc        <  3:319388/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                              
GAAGATGCGAAGGTGTTAGCAGAGCAGGCTCTTGCTCAACCGACAACGGACGAGTTAATGACGCTGGTTAACAAGTTCATTGAAGAAAAAACAATCTGCTAATCCACGAGATGCGGCCCAATTTACTGCTTAGGAGAAGATCATGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTC  >  NC_000913/2535692‑2536234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: