Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	9,907	A→C	6.4%	intergenic (+14/+21)	mog → / ← satP	molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	9,907	0	A	C	6.4%	263.8 / 9.4	78	intergenic (+14/+21)	mog/satP	molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
Reads supporting (aligned to +/- strand):  ref base A (39/34);  new base C (3/2);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATT  >  NC_000913/9762‑10051                                                                                                                                                   |                                                                                                                                                 gTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCg                                                                                                                                               >  4:322248/1‑149 (MQ=255) gTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCg                                                                                                                                               >  1:226420/1‑149 (MQ=255) gTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCg                                                                                                                                               >  1:448070/1‑149 (MQ=255) gTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCg                                                                                                                                               >  1:607781/1‑149 (MQ=255)       gTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGa                                                                                                                                         >  1:581148/1‑149 (MQ=255)       gTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGa                                                                                                                                         >  3:416836/1‑149 (MQ=255)        tACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGAt                                                                                                                                        <  4:588360/149‑1 (MQ=255)        tACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGAt                                                                                                                                        >  2:122696/1‑149 (MQ=255)        tACACGGTATTCTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGAt                                                                                                                                        >  1:329698/1‑149 (MQ=255)         acacGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGGGGAtt                                                                                                                                       <  4:284401/149‑2 (MQ=255)         acacGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGCTCGGGGGGAtt                                                                                                                                       <  1:251951/149‑2 (MQ=255)            cGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCa                                                                                                                                    >  4:412351/1‑149 (MQ=255)              gTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCaaa                                                                                                                                  >  2:618770/1‑149 (MQ=255)                  ttttGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACaa                                                                                                                              >  2:29942/1‑149 (MQ=255)                   tttGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATCAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAAt                                                                                                                             <  4:645446/149‑1 (MQ=255)                      gCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAg                                                                                                                          <  1:262099/149‑1 (MQ=255)                          gCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTggg                                                                                                                      >  4:232748/1‑149 (MQ=255)                           cgtaccgtacTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGa                                                                                                                     <  1:497060/149‑1 (MQ=255)                                cgtacTGCATTCAGTTGTTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCAc                                                                                                                >  1:404289/1‑149 (MQ=255)                                  tacTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCa                                                                                                              >  1:151438/1‑149 (MQ=255)                                   acTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCaa                                                                                                             <  3:604696/149‑1 (MQ=255)                                     tGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGCGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATc                                                                                                           <  4:372077/149‑1 (MQ=255)                                        aTTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTa                                                                                                                           >  2:455313/1‑130 (MQ=255)                                        aTTCAGTTGCTAGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTa                                                                                                                           <  1:455313/130‑1 (MQ=255)                                             gTTGCTGGAAGGGCCTTCCTTGGAAACGCAACGGGAGGTGGTTCAACCTTTCAAACC‑AAAAGTTCCAGAACCCGCCGTTAGGGAATAAAAAATTCCCCCCGAGCGGGGGATTTTCAAAACAATAAGTGGGATTCACCAATCGGCAGacg                                                                                                   >  4:199502/1‑147 (MQ=255)                                             gTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAAc                                                                                                   >  1:117975/1‑149 (MQ=255)                                                gCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGt                                                                                                <  2:226420/149‑1 (MQ=255)                                                  tGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGc                                                                                              >  1:347400/1‑149 (MQ=255)                                                  tGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGc                                                                                              <  2:347400/149‑1 (MQ=255)                                                         gCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCaaa                                                                                       >  3:184138/1‑149 (MQ=255)                                                         gCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCaaa                                                                                       <  1:29942/149‑1 (MQ=255)                                                         gCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCaaa                                                                                       <  2:581148/149‑1 (MQ=255)                                                             tACGTTGAAGCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGCCAGAACGGTGCGACCAAACTGc                                                                                   >  1:602875/1‑149 (MQ=255)                                                             tACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCa                                                                                                                 >  1:11757/1‑119 (MQ=255)                                                             tACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCa                                                                                                                 <  2:11757/119‑1 (MQ=255)                                                              aCGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATc                                                                                                           <  1:150354/124‑1 (MQ=255)                                                              aCGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATc                                                                                                           >  2:150354/1‑124 (MQ=255)                                                                gTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCg                                                                                >  1:189027/1‑149 (MQ=255)                                                                gTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCg                                                                                >  4:116650/1‑149 (MQ=255)                                                                 ttGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGt                                                                               >  2:632284/1‑149 (MQ=255)                                                                 ttGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCCACCAAACTGCTCGt                                                                               >  1:579039/1‑149 (MQ=255)                                                                  tGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGtt                                                                              <  3:322248/149‑1 (MQ=255)                                                                    aaaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCa                                                                            <  1:414896/149‑1 (MQ=255)                                                                    aaaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCa                                                                            <  3:116650/149‑1 (MQ=255)                                                                    aaaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCa                                                                            >  4:271187/1‑149 (MQ=255)                                                                    aaaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCa                                                                            <  1:474269/149‑1 (MQ=255)                                                                    aaaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCa                                                                            <  3:412351/149‑1 (MQ=255)                                                                     aaCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAg                                                                           <  1:632284/149‑1 (MQ=255)                                                                      aCGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGt                                                                          >  3:565825/1‑149 (MQ=255)                                                                        ggCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTAc                                                                        >  4:143260/1‑149 (MQ=255)                                                                        ggCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTAc                                                                        <  2:117975/149‑1 (MQ=255)                                                                        ggCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTAc                                                                        <  2:233756/149‑1 (MQ=255)                                                                               gAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCAccc                                                                 >  4:552690/1‑149 (MQ=255)                                                                                      ttGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGAc                                                                                           >  1:6983/1‑116 (MQ=255)                                                                                      ttGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGAc                                                                                           <  2:6983/116‑1 (MQ=255)                                                                                      ttGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCa                                                          <  2:346780/149‑1 (MQ=255)                                                                                      ttGCAGCATTCAGACCGAAGAGTGCAAG‑AAGCGACGTCAGCGAATAAAAGAATCCCACCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTTACCAAGCGGCAGAACGGTGCCACCAAACTGCTCGTTCAGTACTTCACCCATCCCCa                                                          <  2:208205/149‑1 (MQ=255)                                                                                           gcaTTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCagatag                                                     >  3:14188/1‑149 (MQ=255)                                                                                            caTTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCagataga                                                    <  2:151438/149‑1 (MQ=255)                                                                                             aTTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCagatagat                                                   <  1:108542/149‑1 (MQ=255)                                                                                                  gACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTgcgc                                              >  1:651950/1‑149 (MQ=255)                                                                                                        agagTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCAc                                        <  2:602875/149‑1 (MQ=255)                                                                                                                 g‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCa                               >  4:432545/1‑149 (MQ=255)                                                                                                                 g‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCa                               <  3:124192/149‑1 (MQ=255)                                                                                                                    cgcgACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGc                             <  2:250333/149‑1 (MQ=255)                                                                                                                     gcgACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGcc                            >  3:323982/1‑149 (MQ=255)                                                                                                                                     aaaaaaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGGGGGATTCACCAATCGGAAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCaaa            >  4:356223/1‑149 (MQ=255)                                                                                                                                      aaaaaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAg           >  1:507246/1‑149 (MQ=255)                                                                                                                                       aaaaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGt          <  1:672007/149‑1 (MQ=255)                                                                                                                                         gaTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTgg        >  4:219798/2‑149 (MQ=255)                                                                                                                                         gaTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTgg        >  3:98114/2‑149 (MQ=255)                                                                                                                                          aTCCCCCCGCTCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGga       >  3:334036/1‑149 (MQ=255)                                                                                                                                          aTCCCCCCGAGCGGGGGGAGCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGga       <  4:465056/149‑1 (MQ=255)                                                                                                                                           tCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGgat      <  3:271187/149‑1 (MQ=255)                                                                                                                                              ccccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGgatgat   >  3:191118/1‑149 (MQ=255)                                                                                                                                               cccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTAc                                                                        <  4:124129/79‑1 (MQ=255)                                                                                                                                               cccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTAc                                                                        >  3:124129/1‑79 (MQ=255)                                                                                                                                               cccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGAtt  <  4:674077/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAG‑ACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATT  >  NC_000913/9762‑10051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑