Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,543,803	C→T	5.3%	intergenic (‑275/+29)	cysP ← / ← ucpA	thiosulfate‑binding protein/furfural resistance protein, putative short‑chain oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,543,803	0	C	T	5.3%	249.6 / 3.1	76	intergenic (‑275/+29)	cysP/ucpA	thiosulfate‑binding protein/furfural resistance protein, putative short‑chain oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (37/35);  new base T (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

GCGTAATTGTTGCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCC  >  NC_000913/2543659‑2543949                                                                                                                                                 |                                                                                                                                                   gCGTAATTGTTGCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACaa                                                                                                                                                >  1:681325/1‑149 (MQ=255)   gTAATTGTTGCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAAt                                                                                                                                              >  4:88082/1‑149 (MQ=255)   gTAATTGTTGCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAAt                                                                                                                                              >  3:650478/1‑149 (MQ=255)   gTAATTGTTGCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTAAACATAGTACGTGATGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAAt                                                                                                                                              >  2:492623/1‑149 (MQ=255)        tgttgCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAgg                                                                                                                                         <  2:281640/149‑1 (MQ=255)         gttgCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAggg                                                                                                                                        <  2:125957/149‑1 (MQ=255)           tgCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGggag                                                                                                                                      <  3:141170/149‑1 (MQ=255)              agtcagtTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGaa                                                                                                                                   <  4:258548/149‑1 (MQ=255)               gtcagtTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGaaa                                                                                                                                  <  2:250254/149‑1 (MQ=255)                 cagtTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACa                                                                                                                                <  4:389137/149‑1 (MQ=255)                   gtTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACaga                                                                                                                              <  1:590424/149‑1 (MQ=255)                    tttGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACagag                                                                                                                             >  2:641055/1‑149 (MQ=255)                    tttGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACagag                                                                                                                             <  2:352681/149‑1 (MQ=255)                    tttGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACagag                                                                                                                             <  1:563178/149‑1 (MQ=255)                    tttGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACagag                                                                                                                             <  2:291982/149‑1 (MQ=255)                              aaCAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGa                                                                                                                   <  4:487656/149‑1 (MQ=255)                              aaCAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGa                                                                                                                   <  2:685763/149‑1 (MQ=255)                              aaCAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGa                                                                                                                   <  1:641055/149‑1 (MQ=255)                                     cgGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGAc                                                                                                            >  4:649362/1‑149 (MQ=255)                                      ggAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACg                                                                                                           <  3:649362/149‑1 (MQ=255)                                       gagaAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGc                                                                                                          >  3:405300/1‑149 (MQ=255)                                                   cGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCg                                                                                              <  1:485805/149‑1 (MQ=255)                                                    gTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCgg                                                                                             >  3:395643/1‑149 (MQ=255)                                                    gTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCgg                                                                                             <  2:348538/149‑1 (MQ=255)                                                     tACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGc                                                                                            >  1:662281/1‑149 (MQ=255)                                                      acacATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCa                                                                                           >  3:207280/1‑149 (MQ=255)                                                       cacaTAGTACGTGAGGATTTCGAGCACTGCCCTTGGCCAAAATGACAAGTAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAGAGGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAg                                                                                          >  4:658937/1‑149 (MQ=255)                                                        acaTAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAgt                                                                                         >  1:432309/1‑149 (MQ=255)                                                        acaTAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAgt                                                                                         >  4:659244/1‑149 (MQ=255)                                                          aTAGTACGTGAGGATTACGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGATCAAGACGAACTCCTCTCCAGAAATGCAGGGAGGAAACAGAGGGGAATCATATCGCGACGCTAACCGTCTCCGGCAgtgt                                                                                       >  4:572080/1‑149 (MQ=37)                                                                  tGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTgccgc                                                                               <  2:349374/149‑1 (MQ=255)                                                                        tttCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCatcaa                                                                         >  1:561320/1‑149 (MQ=255)                                                                        tttCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCatcaa                                                                         >  2:700739/1‑149 (MQ=255)                                                                        ttgctaGCACTGCCCGGGCCCAAAATGACAAATAAAATAGCCTGGCGAACATAGCTCGATACGAAAAACTCCCCACAAATGCAGTCAGGACCCAGATTGGACGCAGCTCCCGGCGCGTACGGGCCCCGGCGGCGTGCTTCCGCCatcac                                                                         >  4:1081/6‑148 (MQ=255)                                                                         ttCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCatcaat                                                                        <  3:383143/149‑1 (MQ=255)                                                                         ttCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCatcaat                                                                        <  2:163197/149‑1 (MQ=255)                                                                             aGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATcaca                                                                    >  3:355981/1‑149 (MQ=255)                                                                               cACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACAtt                                                                  <  3:199200/149‑1 (MQ=255)                                                                                 cTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCt                                                                <  2:668534/149‑1 (MQ=255)                                                                                  tGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTgccg                                                                                >  2:124135/1‑132 (MQ=255)                                                                                  tGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTgccg                                                                                <  1:124135/132‑1 (MQ=255)                                                                                  tGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCtg                                                               >  1:327458/1‑149 (MQ=255)                                                                                   gaccGGGGTCAAAATGACAAATAAAATAGCCTGATGAACTTAGTTCAAGACGAAATCCTCCCCGCAAATGCAGGGAGGAAACAGAGGAAAATCAGATACCGACGCTAACCGTCTCCGGCAGAGTGCTTCCGCCATCAATCACAATCtgt                                                              <  4:325197/147‑1 (MQ=255)                                                                                    cccGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCtgtg                                                             <  1:597281/149‑1 (MQ=255)                                                                                           ccAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGt                                                      <  3:94004/149‑1 (MQ=255)                                                                                                aTGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCGCCCCACAAATGCAGGGAGGAAACAGCGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAAt                                                 <  4:507923/149‑1 (MQ=255)                                                                                                  gACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAg                                               <  3:65110/149‑1 (MQ=255)                                                                                                    cAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCt                                             <  4:634287/149‑1 (MQ=255)                                                                                                      aataaaataGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTgg                                           >  1:338448/1‑149 (MQ=255)                                                                                                      aataaaataGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTgg                                           <  2:137653/149‑1 (MQ=255)                                                                                                       ataaaataGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGa                                          >  1:415380/1‑149 (MQ=255)                                                                                                             taGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATc                                    <  1:159738/149‑1 (MQ=255)                                                                                                               gCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGtctc                                                                                                >  1:559266/1‑87 (MQ=255)                                                                                                               gCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGtctc                                                                                                <  2:559266/87‑1 (MQ=255)                                                                                                                 cTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGAt                                <  3:243591/149‑1 (MQ=255)                                                                                                                        aCTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGa                         >  3:586773/1‑149 (MQ=255)                                                                                                                          ttAGTTCGAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTGCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGTATTCATCCGATGCGAGGAAg                       >  3:23574/1‑149 (MQ=255)                                                                                                                          ttAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAg                       >  1:83997/1‑149 (MQ=255)                                                                                                                          ttAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAg                       >  4:20637/1‑149 (MQ=255)                                                                                                                          ttAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAg                       >  2:413705/1‑149 (MQ=255)                                                                                                                             gTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGgcc                    <  4:207280/149‑1 (MQ=255)                                                                                                                              ttCGAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACTGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGAGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGgcca                   >  4:66240/1‑148 (MQ=255)                                                                                                                               tcacctctgttTCCTCCCTGCATTTGTGGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTc                                                                 <  1:629941‑M1/91‑1 (MQ=255)                                                                                                                               tcacctctgttTCCTCCCTGCATTTGTGGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTc                                                                 >  2:629941‑M1/12‑102 (MQ=255)                                                                                                                                cAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGgccgcc                 >  2:539559/1‑149 (MQ=255)                                                                                                                                  aGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAg               >  4:509364/1‑149 (MQ=255)                                                                                                                                   gACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCt                                             <  1:321313/118‑1 (MQ=255)                                                                                                                                   gACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCt                                             >  2:321313/1‑118 (MQ=255)                                                                                                                                   gACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGt              >  4:328483/1‑149 (MQ=255)                                                                                                                                   gACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGt              >  3:530669/1‑149 (MQ=255)                                                                                                                                   ctctgttTCCTCCCTGCATTTGTGGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGa                                          <  4:301771‑M1/114‑1 (MQ=255)                                                                                                                                   ctctgttTCCTCCCTGCATTTGTGGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGa                                          >  3:301771‑M1/8‑121 (MQ=255)                                                                                                                                          TCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGa       >  4:257986‑M1/1‑149 (MQ=255)                                                                                                                                           CCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGAc      >  3:241465‑M1/1‑149 (MQ=255)                                                                                                                                             TCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACtt    <  2:198149‑M1/149‑1 (MQ=255)                                                                                                                                               CCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTcc  >  2:415380‑M1/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GCGTAATTGTTGCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCC  >  NC_000913/2543659‑2543949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑