Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,543,812	A→G	5.5%	intergenic (‑284/+20)	cysP ← / ← ucpA	thiosulfate‑binding protein/furfural resistance protein, putative short‑chain oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,543,812	0	A	G	5.5%	225.9 / 3.1	73	intergenic (‑284/+20)	cysP/ucpA	thiosulfate‑binding protein/furfural resistance protein, putative short‑chain oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (34/35);  new base G (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

TGTTGCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGC  >  NC_000913/2543666‑2543960                                                                                                                                                   |                                                                                                                                                     tgttgCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAgg                                                                                                                                                    <  2:281640/149‑1 (MQ=255)  gttgCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAggg                                                                                                                                                   <  2:125957/149‑1 (MQ=255)    tgCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGggag                                                                                                                                                 <  3:141170/149‑1 (MQ=255)       agtcagtTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGaa                                                                                                                                              <  4:258548/149‑1 (MQ=255)        gtcagtTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGaaa                                                                                                                                             <  2:250254/149‑1 (MQ=255)          cagtTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACa                                                                                                                                           <  4:389137/149‑1 (MQ=255)            gtTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACaga                                                                                                                                         <  1:590424/149‑1 (MQ=255)             tttGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACagag                                                                                                                                        <  2:352681/149‑1 (MQ=255)             tttGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACagag                                                                                                                                        >  2:641055/1‑149 (MQ=255)             tttGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACagag                                                                                                                                        <  2:291982/149‑1 (MQ=255)             tttGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACagag                                                                                                                                        <  1:563178/149‑1 (MQ=255)                       aaCAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGa                                                                                                                              <  2:685763/149‑1 (MQ=255)                       aaCAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGa                                                                                                                              <  1:641055/149‑1 (MQ=255)                       aaCAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGa                                                                                                                              <  4:487656/149‑1 (MQ=255)                              cgGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGAc                                                                                                                       >  4:649362/1‑149 (MQ=255)                               ggAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACg                                                                                                                      <  3:649362/149‑1 (MQ=255)                                gagaAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGc                                                                                                                     >  3:405300/1‑149 (MQ=255)                                            cGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCg                                                                                                         <  1:485805/149‑1 (MQ=255)                                             gTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCgg                                                                                                        <  2:348538/149‑1 (MQ=255)                                             gTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCgg                                                                                                        >  3:395643/1‑149 (MQ=255)                                              tACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGc                                                                                                       >  1:662281/1‑149 (MQ=255)                                               acacATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCa                                                                                                      >  3:207280/1‑149 (MQ=255)                                                cacaTAGTACGTGAGGATTTCGAGCACTGCCCTTGGCCAAAATGACAAGTAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAGAGGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAg                                                                                                     >  4:658937/1‑149 (MQ=255)                                                 acaTAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAgt                                                                                                    >  1:432309/1‑149 (MQ=255)                                                 acaTAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAgt                                                                                                    >  4:659244/1‑149 (MQ=255)                                                   aTAGTACGTGAGGATTACGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGATCAAGACGAACTCCTCTCCAGAAATGCAGGGAGGAAACAGAGGGGAATCATATCGCGACGCTAACCGTCTCCGGCAgtgt                                                                                                  >  4:572080/1‑149 (MQ=37)                                                           tGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTgccgc                                                                                          <  2:349374/149‑1 (MQ=255)                                                                 tttCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCatcaa                                                                                    >  2:700739/1‑149 (MQ=255)                                                                 tttCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCatcaa                                                                                    >  1:561320/1‑149 (MQ=255)                                                                 ttgctaGCACTGCCCGGGCCCAAAATGACAAATAAAATAGCCTGGCGAACATAGCTCGATACGAAAAACTCCCCACAAATGCAGTCAGGACCCAGATTGGACGCAGCTCCCGGCGCGTACGGGCCCCGGCGGCGTGCTTCCGCCatcac                                                                                    >  4:1081/6‑148 (MQ=255)                                                                  ttCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCatcaat                                                                                   <  2:163197/149‑1 (MQ=255)                                                                  ttCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCatcaat                                                                                   <  3:383143/149‑1 (MQ=255)                                                                      aGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATcaca                                                                               >  3:355981/1‑149 (MQ=255)                                                                        cACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACAtt                                                                             <  3:199200/149‑1 (MQ=255)                                                                          cTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCt                                                                           <  2:668534/149‑1 (MQ=255)                                                                           tGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTgccg                                                                                           >  2:124135/1‑132 (MQ=255)                                                                           tGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTgccg                                                                                           <  1:124135/132‑1 (MQ=255)                                                                           tGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCtg                                                                          >  1:327458/1‑149 (MQ=255)                                                                            gaccGGGGTCAAAATGACAAATAAAATAGCCTGATGAACTTAGTTCAAGACGAAATCCTCCCCGCAAATGCAGGGAGGAAACAGAGGAAAATCAGATACCGACGCTAACCGTCTCCGGCAGAGTGCTTCCGCCATCAATCACAATCtgt                                                                         <  4:325197/147‑1 (MQ=255)                                                                             cccGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCtgtg                                                                        <  1:597281/149‑1 (MQ=255)                                                                                    ccAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGt                                                                 <  3:94004/149‑1 (MQ=255)                                                                                         aTGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCGCCCCACAAATGCAGGGAGGAAACAGCGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAAt                                                            <  4:507923/149‑1 (MQ=255)                                                                                           gACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAg                                                          <  3:65110/149‑1 (MQ=255)                                                                                             cAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCt                                                        <  4:634287/149‑1 (MQ=255)                                                                                               aataaaataGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTgg                                                      <  2:137653/149‑1 (MQ=255)                                                                                               aataaaataGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTgg                                                      >  1:338448/1‑149 (MQ=255)                                                                                                ataaaataGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGa                                                     >  1:415380/1‑149 (MQ=255)                                                                                                      taGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATc                                               <  1:159738/149‑1 (MQ=255)                                                                                                        gCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGtctc                                                                                                           >  1:559266/1‑87 (MQ=255)                                                                                                        gCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGtctc                                                                                                           <  2:559266/87‑1 (MQ=255)                                                                                                          cTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGAt                                           <  3:243591/149‑1 (MQ=255)                                                                                                                 aCTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGa                                    >  3:586773/1‑149 (MQ=255)                                                                                                                   ttAGTTCGAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTGCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGTATTCATCCGATGCGAGGAAg                                  >  3:23574/1‑149 (MQ=255)                                                                                                                   ttAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAg                                  >  1:83997/1‑149 (MQ=255)                                                                                                                   ttAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAg                                  >  4:20637/1‑149 (MQ=255)                                                                                                                   ttAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAg                                  >  2:413705/1‑149 (MQ=255)                                                                                                                      gTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGgcc                               <  4:207280/149‑1 (MQ=255)                                                                                                                       ttCGAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACTGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGAGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGgcca                              >  4:66240/1‑148 (MQ=255)                                                                                                                        tcacctctgttTCCTCCCTGCATTTGTGGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTc                                                                            <  1:629941‑M1/91‑1 (MQ=255)                                                                                                                        tcacctctgttTCCTCCCTGCATTTGTGGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTc                                                                            >  2:629941‑M1/12‑102 (MQ=255)                                                                                                                         cAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGgccgcc                            >  2:539559/1‑149 (MQ=255)                                                                                                                           aGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAg                          >  4:509364/1‑149 (MQ=255)                                                                                                                            gACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCt                                                        >  2:321313/1‑118 (MQ=255)                                                                                                                            gACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCt                                                        <  1:321313/118‑1 (MQ=255)                                                                                                                            gACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGt                         >  4:328483/1‑149 (MQ=255)                                                                                                                            gACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGt                         >  3:530669/1‑149 (MQ=255)                                                                                                                            ctctgttTCCTCCCTGCATTTGTGGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGa                                                     >  3:301771‑M1/8‑121 (MQ=255)                                                                                                                            ctctgttTCCTCCCTGCATTTGTGGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGa                                                     <  4:301771‑M1/114‑1 (MQ=255)                                                                                                                                   TCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGa                  >  4:257986‑M1/1‑149 (MQ=255)                                                                                                                                    CCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGAc                 >  3:241465‑M1/1‑149 (MQ=255)                                                                                                                                      TCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACtt               <  2:198149‑M1/149‑1 (MQ=255)                                                                                                                                        CCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTcc             >  2:415380‑M1/1‑149 (MQ=255)                                                                                                                                           acaAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGc          >  2:589194/1‑149 (MQ=255)                                                                                                                                                   aGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCggc  <  1:198149/149‑1 (MQ=255)                                                                                                                                                   aGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCggc  >  3:266296/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     TGTTGCAGTCAGTTTGGACACGAACAGCGCGGAGAAACCGGAGCGTACACATAGTACGTGAGGATTTCGAGCACTGCCCGGGGCCAAAATGACAAATAAAATAGCCTGGTGAACTTAGTTCAAGACGAAATCCTCCCCACAAATGCAGGGAGGAAACAGAGGTGAATCAGATACCGACGCTAACCGTCTCCGGCAGTGTGCTGCCGCCATCAATCACATTCTGTGTACCGGTTAAATAGCTGGATTCATCCGATGCGAGGAAGGCCGCCAGTTCGCCGACTTCCAGCGGATCGGC  >  NC_000913/2543666‑2543960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑