Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,317 A→C 6.3% N21H (AAT→CAT)  yffL → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,3170AC 6.3% 271.9 / 10.9 80N21H (AAT→CAT) yffLCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (41/34);  new base C (2/3);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

CGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCA  >  NC_000913/2560170‑2560461
                                                                                                                                                   |                                                                                                                                                
cGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGaa                                                                                                                                                 <  3:57015/149‑1 (MQ=255)
cGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGaa                                                                                                                                                 <  1:556270/149‑1 (MQ=255)
 ggATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAt                                                                                                                                                >  4:503252/1‑149 (MQ=255)
 ggATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAt                                                                                                                                                <  1:358269/149‑1 (MQ=255)
       gTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCa                                                                                                                                          >  4:273170/1‑149 (MQ=255)
        tCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCaa                                                                                                                                         >  3:552150/1‑149 (MQ=255)
          cGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACa                                                                                                                                       >  1:558243/1‑149 (MQ=255)
              ttttcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGcc                                                                                                                                   <  3:667545/149‑1 (MQ=255)
                ttcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgg                                                                                                                                 <  1:562216/149‑1 (MQ=255)
                 tcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGCACTCAAATCCACGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                                                                <  2:499636/149‑1 (MQ=255)
                  cttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCgggg                                                                                                                               >  1:395400/1‑149 (MQ=255)
                   tttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggggg                                                                                                                              >  2:304081/1‑149 (MQ=255)
                   tttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggggg                                                                                                                              >  2:222277/1‑149 (MQ=255)
                     tttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCg                                                                                                                            <  3:114789/149‑1 (MQ=255)
                      ttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                                                           <  1:457395/149‑1 (MQ=255)
                      ttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                                                           <  1:371611/149‑1 (MQ=255)
                           tAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATga                                                                                                                      <  1:550487/149‑1 (MQ=255)
                               tattccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGaaa                                                                                                                  <  4:661068/149‑1 (MQ=255)
                                attccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAAc                                                                                                                 <  2:564619/149‑1 (MQ=255)
                                attccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAAc                                                                                                                 <  3:375093/149‑1 (MQ=255)
                                  tccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACaa                                                                                                               <  4:508480/149‑1 (MQ=255)
                                    cattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGATTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGc                                                                                                             <  2:525177/149‑1 (MQ=255)
                                     attattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATgaga                                                                                                                    <  4:533438/141‑1 (MQ=255)
                                     attattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATgaga                                                                                                                    >  3:533438/1‑141 (MQ=255)
                                       tattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTaa                                                                                                          <  4:5244/149‑1 (MQ=255)
                                        attccaATTAAGTGGAATAAAGTACGCAACATCAAGAACTTAAATCCATGTTTGCGACGAAAGATCCAGAGATCGAAAACAGGATAAACACAAATAAAAGCCCCCTGAATGCAGCAACATGCCTGGGGCGGTATGAGAAACAAGCTaaa                                                                                                         >  4:424231/1‑149 (MQ=255)
                                         ttccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAg                                                                                                        >  1:516081/1‑149 (MQ=255)
                                            caATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGCGCGGTATGAGAAACAAGCTAAAGgag                                                                                                     >  4:567179/1‑149 (MQ=255)
                                              aTTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                                                                   <  3:650148/149‑1 (MQ=255)
                                                 aaGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAAttt                                                                                                <  2:219356/149‑1 (MQ=255)
                                                  aGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtc                                                                                               <  3:501689/149‑1 (MQ=255)
                                                    tGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtctc                                                                                             >  1:464198/1‑149 (MQ=255)
                                                     ggAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                                                            >  4:395739/1‑149 (MQ=255)
                                                       aaTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATg                                                                                          >  4:372500/1‑149 (MQ=255)
                                                         tgaaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCt                                                                                       >  3:3027/3‑149 (MQ=255)
                                                           aaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTg                                                                                      >  4:230491/1‑149 (MQ=255)
                                                            attattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATGCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGa                                                                                     <  2:516081/149‑1 (MQ=255)
                                                            attattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGa                                                                                     >  3:410619/1‑149 (MQ=255)
                                                            attattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGa                                                                                     >  3:92859/1‑149 (MQ=255)
                                                              tattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTcc                                                                                                                                   <  1:627134/101‑1 (MQ=255)
                                                              tattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTcc                                                                                                                                   >  2:627134/1‑101 (MQ=255)
                                                                  cAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCt                                                                               <  3:165418/149‑1 (MQ=255)
                                                                   aaTATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                                                              <  2:513864/149‑1 (MQ=255)
                                                                   aaTATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                                                              <  1:299184/149‑1 (MQ=255)
                                                                      atCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                                                                   <  3:549009/125‑1 (MQ=255)
                                                                      atCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                                                                   >  4:549009/1‑125 (MQ=255)
                                                                           gAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGa                                                                      <  2:25463/149‑1 (MQ=255)
                                                                            aaCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGaa                                                                     >  3:89357/1‑149 (MQ=255)
                                                                               ttAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCGTGTCTGATATGCTAGCAGTAGAACaa                                                                  >  3:477570/1‑149 (MQ=255)
                                                                               ttAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACaa                                                                  >  1:3890/1‑149 (MQ=255)
                                                                               ttAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACaa                                                                  >  1:606062/1‑149 (MQ=255)
                                                                               ttAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTCCTCATGTCTGATATGCTAGCAGTAGAACaa                                                                  <  1:370033/149‑1 (MQ=255)
                                                                                     ccATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAg                                                                                                        <  3:653797/105‑1 (MQ=255)
                                                                                     ccATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAg                                                                                                        >  4:653797/1‑105 (MQ=255)
                                                                                            tGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatg                                                     <  1:385683/149‑1 (MQ=255)
                                                                                              cAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatgat                                                   <  4:483608/149‑1 (MQ=255)
                                                                                                aCGAAAGATCCAGAGTTCGAAAAGAGAATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGt                                                 <  3:395739/149‑1 (MQ=255)
                                                                                                  gAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTc                                               >  3:222466/1‑149 (MQ=255)
                                                                                                   aaaGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGTGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCg                                              >  1:528451/1‑149 (MQ=255)
                                                                                                     aGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTc                                            >  3:357555/1‑149 (MQ=255)
                                                                                                     aGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTc                                            <  3:260205/149‑1 (MQ=255)
                                                                                                       aTCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCaa                                          >  4:245365/1‑149 (MQ=255)
                                                                                                           agagTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAtttt                                      >  3:353166/1‑149 (MQ=255)
                                                                                                               ttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                  >  4:508780/1‑149 (MQ=255)
                                                                                                               ttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                  >  1:481942/1‑149 (MQ=255)
                                                                                                               ttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                  >  1:229583/1‑149 (MQ=255)
                                                                                                               ttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATACCGGGGGCGGTATGAGAAACAAGCTAAAGGAGACTTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                  >  3:451826/1‑149 (MQ=255)
                                                                                                                    aaaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaaaa                             <  4:246488/149‑1 (MQ=255)
                                                                                                                    aaaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaaaa                             <  2:3890/149‑1 (MQ=255)
                                                                                                                         ggATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAAc                        <  2:558243/149‑1 (MQ=255)
                                                                                                                          gATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACg                       >  1:310777/1‑149 (MQ=255)
                                                                                                                           aTCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                                                             <  2:460313/94‑1 (MQ=255)
                                                                                                                           aTCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                                                             >  1:460313/1‑94 (MQ=255)
                                                                                                                               acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTa                  >  1:128858/1‑149 (MQ=255)
                                                                                                                                cacaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTAc                 <  1:304081/149‑1 (MQ=255)
                                                                                                                                     aTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAAt            >  2:239766/1‑149 (MQ=255)
                                                                                                                                         aaGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAg        >  1:294958/1‑149 (MQ=255)
                                                                                                                                             ccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAcc    >  1:500757/1‑149 (MQ=255)
                                                                                                                                             ccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAcc    >  1:545049/1‑149 (MQ=255)
                                                                                                                                               ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAg                                                                 >  4:237611/1‑86 (MQ=255)
                                                                                                                                               ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAg                                                                 <  3:237611/86‑1 (MQ=255)
                                                                                                                                               ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCa  >  1:82754/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCA  >  NC_000913/2560170‑2560461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: