Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,636,747	T→C	7.6%	T222A (ACC→GCC)	yhiN ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,747	0	T	C	7.6%	281.8 / 11.1	92	T222A (ACC→GCC)	yhiN	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (35/50);  new base C (2/5);  total (37/55)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

CGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATA  >  NC_000913/3636599‑3636891                                                                                                                                                     |                                                                                                                                                 cGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGt                                                                                                                                                  <  3:503598/149‑1 (MQ=255)    tgctCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGcc                                                                                                                                               <  1:376333/149‑1 (MQ=255)         aTTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGtttt                                                                                                                                          >  4:653210/1‑149 (MQ=255)          ttCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTc                                                                                                                                         >  4:543934/1‑149 (MQ=255)          ttCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTc                                                                                                                                         <  2:187192/149‑1 (MQ=255)             aGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGc                                                                                                                                      >  1:456938/1‑149 (MQ=255)             aGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTccgt                                                                                                                                      <  1:417997/149‑4 (MQ=255)             aGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTccgt                                                                                                                                      <  4:264258/149‑4 (MQ=255)               gAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCgg                                                                                                                                    >  1:91206/1‑149 (MQ=255)                     ttCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCa                                                                                                                              <  1:465027/149‑1 (MQ=255)                      tCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCAc                                                                                                                             <  1:510517/149‑1 (MQ=255)                         aGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGa                                                                                                                          <  4:57972/149‑1 (MQ=255)                          ggTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGaa                                                                                                                         <  3:237952/149‑1 (MQ=255)                            tCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAgg                                                                                                                       >  1:259768/1‑149 (MQ=255)                             ccACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGc                                                                                                                      >  3:662622/1‑149 (MQ=255)                              cacaTCCGGTGGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCa                                                                                                                     >  3:43617/1‑149 (MQ=255)                              cacaTCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTATCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGAGGGTGAAGAGTAAGCTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCa                                                                                                                     >  2:100347/1‑149 (MQ=255)                              cacaTCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCa                                                                                                                     >  1:218570/1‑149 (MQ=255)                              cacaTCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCa                                                                                                                     >  3:455320/1‑149 (MQ=255)                                caTCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCGCGGAAAACGGTGCCGTTTTCAGCGGTAGTCACGGAAGGCAcc                                                                                                                   <  3:51773/149‑1 (MQ=255)                                 aTCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCg                                                                                                                  >  1:217978/1‑149 (MQ=255)                                  tCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGc                                                                                                                 <  4:455320/149‑1 (MQ=255)                                  tCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGc                                                                                                                 >  1:661137/1‑149 (MQ=255)                                         gCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgccc                                                                                                          <  4:531987/149‑1 (MQ=255)                                         gCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgccc                                                                                                          <  1:532301/149‑1 (MQ=255)                                         gCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTccg                                                                                                                                       <  1:130979/120‑3 (MQ=255)                                         gCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTccg                                                                                                                                       >  2:130979/1‑118 (MQ=255)                                           aGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgcccgc                                                                                                        >  4:157943/1‑149 (MQ=255)                                            gATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgcccgcc                                                                                                       <  1:232665/149‑1 (MQ=255)                                             aTTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCa                                                                                                      >  1:189705/1‑149 (MQ=255)                                             aTTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCa                                                                                                      <  4:58098/149‑1 (MQ=255)                                                 aTGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCAc                                                                                                  <  4:539225/149‑1 (MQ=255)                                                   gCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCt                                                                                                <  1:513664/149‑1 (MQ=255)                                                   gCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCt                                                                                                <  2:241836/149‑1 (MQ=255)                                                     tGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGt                                                                                              <  2:650229/149‑1 (MQ=255)                                                      gACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgccc                                                                                                          <  2:554033/136‑1 (MQ=255)                                                      gACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCAcgccc                                                                                                          >  1:554033/1‑136 (MQ=255)                                                            ttCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCtt                                                                                       >  2:31529/1‑149 (MQ=255)                                                            ttCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCtt                                                                                       <  3:453432/149‑1 (MQ=255)                                                             tCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTc                                                                                      <  1:656086/149‑1 (MQ=255)                                                               ccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAg                                                                                                                                       >  3:561542/1‑98 (MQ=255)                                                               ccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAg                                                                                                                                       <  4:561542/98‑1 (MQ=255)                                                               ccccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGa                                                                                    >  4:215372/1‑149 (MQ=255)                                                                cccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAg                                                                                   >  1:446902/1‑149 (MQ=255)                                                                 ccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGc                                                                                  >  1:248150/1‑149 (MQ=255)                                                                 ccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTcc                                                                                                                                        >  3:176909/1‑94 (MQ=255)                                                                 ccGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGCACCGTTTTcc                                                                                                                                        <  4:176909/95‑2 (MQ=255)                                                                  cGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCa                                                                                 <  2:679444/149‑1 (MQ=255)                                                                   ggTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCaa                                                                                <  3:416352/149‑1 (MQ=255)                                                                      tGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGGGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTGTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACgg                                                                             <  4:323110/149‑1 (MQ=255)                                                                       gCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGt                                                                            <  1:171581/149‑1 (MQ=255)                                                                         cAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGttt                                                                          <  2:218570/149‑1 (MQ=255)                                                                          aGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTa                                                                         <  2:105128/149‑1 (MQ=255)                                                                              gCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCa                                                                     <  3:229639/149‑1 (MQ=255)                                                                                ttGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCaga                                                                   <  2:415411/149‑1 (MQ=255)                                                                                     aTCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGaa                                                              <  3:441891/149‑1 (MQ=255)                                                                                      tCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCGCGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAAt                                                             <  2:200998/149‑1 (MQ=255)                                                                                      tCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGTCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAAt                                                             <  4:385253/149‑1 (MQ=255)                                                                                      tCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAAt                                                             >  4:506149/1‑149 (MQ=255)                                                                                      tCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCACCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAAt                                                             <  4:214779/149‑1 (MQ=255)                                                                                             cacCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCa                                                      <  2:590181/149‑1 (MQ=255)                                                                                              acCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAg                                                     >  2:658947/1‑149 (MQ=255)                                                                                                cgccgGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGAc                                                   <  2:91206/149‑1 (MQ=255)                                                                                                cgccgGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGAc                                                   <  1:222350/149‑1 (MQ=255)                                                                                                  ccgGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGAccc                                                 <  2:295485/149‑1 (MQ=255)                                                                                                        cAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgctg                                           <  4:619975/149‑3 (MQ=255)                                                                                                        cAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCgg                                           >  1:6401/1‑149 (MQ=255)                                                                                                        cAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCgg                                           >  1:497104/1‑149 (MQ=255)                                                                                                        cAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCgg                                           <  3:591060/149‑1 (MQ=255)                                                                                                          gACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGt                                         >  2:374144/1‑149 (MQ=255)                                                                                                              aGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGgc                                     <  2:456518/149‑1 (MQ=255)                                                                                                               gCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGgca                                    >  1:258467/1‑149 (MQ=255)                                                                                                                      tgggtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                             <  3:657414/149‑1 (MQ=255)                                                                                                                      tgggtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                             <  4:422248/149‑1 (MQ=255)                                                                                                                        ggtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGa                           <  3:417704/149‑1 (MQ=255)                                                                                                                        ggtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTCTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGa                           <  1:272088/149‑1 (MQ=255)                                                                                                                         gtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCGGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAg                          <  3:543934/149‑1 (MQ=255)                                                                                                                          tgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCa                                                                                                   <  2:343524/75‑1 (MQ=255)                                                                                                                          tgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCa                                                                                                   >  1:343524/1‑75 (MQ=255)                                                                                                                          tgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAgg                         >  3:532341/1‑149 (MQ=255)                                                                                                                              gagTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCGTCGAGCAACGGTTTATGCCGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTAAGGCCaa                     >  1:541171/1‑149 (MQ=255)                                                                                                                               agTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCa                                                         >  2:274804/1‑112 (MQ=255)                                                                                                                               agTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCa                                                         <  1:274804/112‑1 (MQ=255)                                                                                                                               agTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCaaa                    >  3:649728/1‑149 (MQ=255)                                                                                                                                 tAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAAtt                  >  4:534104/1‑149 (MQ=255)                                                                                                                                     ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTc              <  4:43617/149‑1 (MQ=255)                                                                                                                                     ttCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTc              <  1:585654/149‑1 (MQ=255)                                                                                                                                      tctcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCg             <  1:142259/149‑1 (MQ=255)                                                                                                                                        tcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGc           >  4:400414/1‑149 (MQ=255)                                                                                                                                        tcACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGc           >  2:498508/1‑149 (MQ=255)                                                                                                                                              aaaaCGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAgg                         <  4:171156/129‑1 (MQ=255)                                                                                                                                              aaaaCGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAgg                         >  3:171156/1‑129 (MQ=255)                                                                                                                                                 aCGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTtata  <  3:482703/149‑1 (MQ=255)                                                                                                                                                     tGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCgg                                           <  1:535364/104‑1 (MQ=255)                                                                                                                                                     tGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCgg                                           >  2:535364/1‑104 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGCTGCTCATTCAGGAAGGTTTCGAGGTCCACATCCGGTAGCAGATTGATGCTGACAAATTCCCCCGGTTGCCAGTAGCTTGAAATCTGCAACACCGCCGGTCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATA  >  NC_000913/3636599‑3636891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑