BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,117,047	G→T	93.7%	S59Y (TCC→TAC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,047	0	G	T	93.7%	371.9 / 14.8	111	S59Y (TCC→TAC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (3/4); new base T (48/56); total (51/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 112 total aligned reads displayed.

GGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTC  >  NC_000913/4116904‑4117192

ggCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCtt                                                                                                                                              >  2:443811/1‑149 (MQ=255)

ggCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCtt                                                                                                                                              <  1:251975/149‑1 (MQ=255)

 gCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCttt                                                                                                                                             <  3:255049/149‑1 (MQ=255)

   aCTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTgg                                                                                                                                           <  1:463973/149‑1 (MQ=255)

    cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTggg                                                                                                                                          <  1:328097/149‑1 (MQ=255)

         aGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGcc                                                                                                                                     >  2:137386/1‑149 (MQ=255)

             aaTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAGTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGATCTGTTATCGGCTTTCGCCAGCACTTCTACCAGTGTGTAGCTTTGGGTGGCCCTGa                                                                                                                                 <  4:424652/149‑1 (MQ=255)

              aTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGAt                                                                                                                                >  1:24839/1‑149 (MQ=255)

               tGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGAtt                                                                                                                               <  2:358325/149‑1 (MQ=255)

                 gCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCttt                                                                                                                                             >  4:497717/1‑133 (MQ=255)

                 gCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCttt                                                                                                                                             <  3:497717/133‑1 (MQ=255)

                  cGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTcc                                                                                                                            <  4:416057/149‑1 (MQ=255)

                          atagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                    >  3:280372/1‑149 (MQ=255)

                            agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt                                                                                                                  >  1:152895/1‑149 (MQ=255)

                            agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt                                                                                                                  <  3:488621/149‑1 (MQ=255)

                            agGCTTGCAGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGAGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt                                                                                                                  <  2:698808/149‑1 (MQ=255)

                              gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGTACTGATATCGGCTCTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                                                                                                 <  4:558002/148‑1 (MQ=255)

                              gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                                                                >  1:552384/1‑149 (MQ=255)

                              gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTCTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                                                                                                 >  3:558002/1‑148 (MQ=255)

                                ttGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTc                                                                                                              >  3:407125/1‑149 (MQ=255)

                                 tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt                                                                                                             <  4:261498/149‑1 (MQ=255)

                                      gTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCa                                                                                                        >  3:237844/1‑149 (MQ=255)

                                       tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                                                                                     >  1:86234/1‑135 (MQ=255)

                                       tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                                                                                     <  2:86234/135‑1 (MQ=255)

                                       tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                                                                                       >  1:534114/1‑149 (MQ=255)

                                       tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa                                                                                                       <  4:213103/149‑1 (MQ=255)

                                           tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                                   >  3:20859/1‑149 (MQ=255)

                                           tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                                   >  2:199725/1‑149 (MQ=255)

                                            ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAGCTgg                                                                                                  >  3:660101/1‑149 (MQ=255)

                                            ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                                                                                                  >  1:303010/1‑149 (MQ=255)

                                              ttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                <  4:111538/149‑1 (MQ=255)

                                                 ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTg                                                                                             <  1:487864/149‑1 (MQ=255)

                                                 ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTg                                                                                             <  3:45390/149‑1 (MQ=255)

                                                 ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTg                                                                                             >  4:21990/1‑149 (MQ=255)

                                                  cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                            <  4:503662/149‑1 (MQ=255)

                                                  cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                            >  4:153582/1‑149 (MQ=255)

                                                   aGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGTTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                            >  1:175302/1‑148 (MQ=255)

                                                   aGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                            <  2:175302/148‑1 (MQ=255)

                                                     aCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTa                                                                                         >  2:387383/1‑149 (MQ=255)

                                                        aTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAt                                                                                      <  4:246366/149‑1 (MQ=255)

                                                         tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAtt                                                                                     >  1:188611/1‑149 (MQ=255)

                                                            tGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGAt                                                                                                                                <  3:70325/103‑1 (MQ=255)

                                                             ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCt                                                                                 <  4:569717/149‑1 (MQ=255)

                                                              gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGAGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTc                                                                                >  3:409690/1‑149 (MQ=255)

                                                                ttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaa                                                                              >  4:295290/1‑149 (MQ=255)

                                                                 tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaaa                                                                             <  2:648538/149‑1 (MQ=255)

                                                                  cACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGCGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAt                                                                            <  3:322649/149‑1 (MQ=255)

                                                                   aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt                                                                           <  3:89610/149‑1 (MQ=255)

                                                                     gCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcg                                                                         <  2:449176/149‑1 (MQ=255)

                                                                       tGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg                                                                       <  3:114038/149‑1 (MQ=255)

                                                                          ttCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg                                                                    >  3:308049/1‑149 (MQ=255)

                                                                           tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                                   <  1:215367/149‑1 (MQ=255)

                                                                           tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCATGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                                   <  3:465547/149‑1 (MQ=255)

                                                                              tAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                                                                >  1:181329/1‑149 (MQ=255)

                                                                              tAATACCGATAACTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                                                                >  3:441354/1‑149 (MQ=255)

                                                                                  aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                            >  2:669864/1‑117 (MQ=255)

                                                                                  aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                            <  1:669864/117‑1 (MQ=255)

                                                                                  aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGc                                                            <  1:199725/149‑1 (MQ=255)

                                                                                    cGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                          >  3:625241/1‑149 (MQ=255)

                                                                                     gATAGCTGCAATTTGATCGGAACTGATCTCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGCTTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                         <  4:409690/149‑1 (MQ=255)

                                                                                     gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                         >  4:259/1‑149 (MQ=255)

                                                                                      aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt                                                        <  2:361621/149‑1 (MQ=255)

                                                                                      aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATATGCTCAAATTCGCGCTGCGACACGCTAAt                                                        <  2:472238/149‑1 (MQ=255)

                                                                                         gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGat                                                     <  1:115337/149‑1 (MQ=255)

                                                                                           tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                   <  2:196068/149‑1 (MQ=255)

                                                                                           tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                   <  3:438360/149‑1 (MQ=255)

                                                                                           tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCCGCGACACGCTAATGatat                                                   >  2:537068/1‑149 (MQ=255)

                                                                                           tGCAACTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                   <  4:441354/149‑1 (MQ=255)

                                                                                             cAATTTGATCGGAACTGATATCGGCTTTCGCCCGCACTTCTCCCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                                                 <  4:409047/149‑1 (MQ=255)

                                                                                              aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg                                                <  4:299795/149‑1 (MQ=255)

                                                                                               aTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGc                                               <  4:248176/149‑1 (MQ=255)

                                                                                                  tGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATc                                            <  2:181329/149‑1 (MQ=255)

                                                                                                    aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGt                                          >  2:403784/1‑149 (MQ=255)

                                                                                                    aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGt                                          <  2:526913/149‑1 (MQ=255)

                                                                                                        gAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATc                                      <  1:356457/149‑1 (MQ=255)

                                                                                                        gAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATc                                      >  3:335241/1‑149 (MQ=255)

                                                                                                         aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATcc                                     <  2:188611/149‑1 (MQ=255)

                                                                                                            tGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCAtt                                  <  1:403784/149‑1 (MQ=255)

                                                                                                              atatCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTAc                                >  4:638121/1‑149 (MQ=255)

                                                                                                              atatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTAc                                >  2:462576/1‑149 (MQ=255)

                                                                                                                 tCGGCTTTGGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                             >  3:629485/1‑149 (MQ=255)

                                                                                                                 tCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                            >  4:182000/1‑86 (MQ=255)

                                                                                                                 tCGGCTTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                             >  3:393529/1‑149 (MQ=255)

                                                                                                                  cGGCTTTCGCCAGCACTTCTGCCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAcc                            <  1:178259/149‑1 (MQ=255)

                                                                                                                          gCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGa                    <  2:193474/149‑1 (MQ=255)

                                                                                                                           ccAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTTTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAg                   >  1:320896/1‑149 (MQ=255)

                                                                                                                             aGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCt                 <  4:130554/149‑1 (MQ=255)

                                                                                                                              gCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtg                <  4:211994/149‑1 (MQ=255)

                                                                                                                              gCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtg                <  1:443811/149‑1 (MQ=255)

                                                                                                                              gCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtg                >  1:126890/1‑149 (MQ=255)

                                                                                                                              gCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtg                <  4:435017/149‑1 (MQ=255)

                                                                                                                               cACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtgg               >  2:582563/1‑149 (MQ=255)

                                                                                                                                 cTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg             <  1:387383/149‑1 (MQ=255)

                                                                                                                                   tCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTggtggt           <  4:308049/149‑1 (MQ=255)

                                                                                                                                     tACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGc         >  3:314901/1‑149 (MQ=255)

                                                                                                                                        cAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTGATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCt      >  1:675624/1‑149 (MQ=255)

                                                                                                                                        cAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCt      >  1:415550/1‑149 (MQ=255)

                                                                                                                                          gCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTgg    >  4:364951/1‑149 (MQ=255)

                                                                                                                                          gCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTgg    >  2:137342/1‑149 (MQ=255)

                                                                                                                                            gtgtAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTc  >  4:625496/1‑149 (MQ=255)

GGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTC  >  NC_000913/4116904‑4117192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`