Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 438,924 G→A 5.3% I418I (ATC→ATT)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913438,9240GA 5.3% 245.7 / 2.7 76I418I (ATC→ATT) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base G (39/33);  new base A (2/2);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

GTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGG  >  NC_000913/438780‑439072
                                                                                                                                                |                                                                                                                                                    
gTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATgg                                                                                                                                                  <  3:447578/149‑1 (MQ=255)
  gATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCg                                                                                                                                                >  1:171928/1‑149 (MQ=255)
      gCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACa                                                                                                                                            >  4:288099/1‑149 (MQ=255)
         ggTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGa                                                                                                                                         >  3:412924/1‑149 (MQ=255)
              agagCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGa                                                                                                                                    >  1:423624/1‑149 (MQ=255)
               gagCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGaa                                                                                                                                   >  3:184823/1‑149 (MQ=255)
               gagCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGaa                                                                                                                                   >  4:293200/1‑149 (MQ=255)
                agCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAg                                                                                                                                  >  4:136080/1‑149 (MQ=255)
                   aTCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCtt                                                                                                                               <  4:484052/149‑1 (MQ=255)
                   aTCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCtt                                                                                                                               >  4:131482/1‑149 (MQ=255)
                   aTCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCAGTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCtt                                                                                                                               >  2:621144/1‑149 (MQ=255)
                     cTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCtttt                                                                                                                             <  1:646514/149‑1 (MQ=255)
                     cTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCtttt                                                                                                                             <  2:235409/149‑1 (MQ=255)
                      tGGTGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTg                                                                                                                            >  4:600426/1‑149 (MQ=255)
                         cGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAAt                                                                                                                         <  3:85456/149‑1 (MQ=255)
                          gACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCTGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTCTTGAATc                                                                                                                        >  4:544372/1‑149 (MQ=255)
                          gACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATc                                                                                                                        <  3:352612/149‑1 (MQ=255)
                             aTTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGcc                                                                                                                     <  3:285857/149‑1 (MQ=255)
                             aTTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCAATGGCGAACAGGACCGGAAGCt                                                                                                                                <  3:90686/138‑1 (MQ=255)
                             aTTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCAATGGCGAACAGGACCGGAAGCt                                                                                                                                <  3:657370/138‑1 (MQ=255)
                             aTTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCAATGGCGAACAGGACCGGAAGCt                                                                                                                                >  4:657370/1‑138 (MQ=255)
                             aTTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCAATGGCGAACAGGACCGGAAGCt                                                                                                                                >  4:90686/1‑138 (MQ=255)
                              ttCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGAc                                                                                                                                        <  4:223121/129‑1 (MQ=255)
                              ttCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGAc                                                                                                                                        >  3:223121/1‑129 (MQ=255)
                              gtCGTTTTCATCGCTCGGGGGCATAATGACAATTTCCGGTATGCAGCGCTGGTAAGAGGGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGCTGGCGAGCAGGACCGGAAGCTTTTGAATCGcga                                                                                                                    >  2:36234/2‑147 (MQ=255)
                               tCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCAc                                                                                                                   >  3:315414/1‑149 (MQ=255)
                               tCGTTTTCATCGCTCGGAGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCAc                                                                                                                   <  3:486846/149‑1 (MQ=255)
                                  ttttCATCGCTCGGGGTCATATTGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTc                                                                                                                >  3:571638/1‑149 (MQ=255)
                                  ttttCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCACGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTc                                                                                                                >  3:620505/1‑149 (MQ=255)
                                    ttCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCAt                                                                                                              >  4:675957/1‑149 (MQ=255)
                                       aTCGCTCGGGGACATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATgca                                                                                                           >  3:386008/1‑149 (MQ=255)
                                           cTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCAc                                                                                                       <  4:184823/149‑1 (MQ=255)
                                               gggTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGa                                                                                                   <  3:639119/149‑1 (MQ=255)
                                                 gTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATc                                                                                                 <  3:597179/149‑1 (MQ=255)
                                                  tCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCa                                                                                                >  4:545814/1‑149 (MQ=255)
                                                    aTAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATa                                                                                              <  2:358994/149‑1 (MQ=255)
                                                      aaTGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAgg                                                                                            >  3:601525/1‑149 (MQ=255)
                                                       aTGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGgc                                                                                           >  1:653855/1‑149 (MQ=255)
                                                        tGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGgcg                                                                                          >  2:100169/1‑149 (MQ=255)
                                                         gACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGgcgc                                                                                         >  3:34045/1‑149 (MQ=255)
                                                         gACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGgcgc                                                                                         >  2:268106/1‑149 (MQ=255)
                                                          aCCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGcgcg                                                                                        <  2:14947/149‑1 (MQ=255)
                                                          aCCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGcgcg                                                                                        <  1:651132/149‑1 (MQ=255)
                                                            cATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGtt                                                                                      >  3:470515/1‑149 (MQ=255)
                                                              tttCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGATCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGc                                                                                    >  1:654267/1‑149 (MQ=255)
                                                              tttCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGc                                                                                    >  4:658696/1‑149 (MQ=255)
                                                              tttCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGc                                                                                    >  3:311658/1‑149 (MQ=255)
                                                               ttCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCa                                                                                   <  1:70728/149‑1 (MQ=255)
                                                                tCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAg                                                                                  <  3:552526/149‑1 (MQ=255)
                                                                    gTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCTTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGATCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGaaa                                                                              <  4:269522/149‑1 (MQ=255)
                                                                         cAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGa                                                                         <  4:486755/149‑1 (MQ=255)
                                                                         cAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGa                                                                         <  1:100169/149‑1 (MQ=255)
                                                                              cAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTaaa                                                                    <  4:601525/149‑1 (MQ=255)
                                                                                         gaTCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATg                                                         <  1:580714/149‑1 (MQ=255)
                                                                                         gaTCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATg                                                         >  2:83819/1‑149 (MQ=255)
                                                                                         gaTCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATg                                                         >  4:402716/1‑149 (MQ=255)
                                                                                           tCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATggg                                                       <  4:311658/149‑1 (MQ=255)
                                                                                              aaaGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGttt                                                    <  3:600426/149‑1 (MQ=255)
                                                                                              aaaGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGttt                                                    >  1:547486/1‑149 (MQ=255)
                                                                                              aaaGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGttt                                                    <  3:109472/149‑1 (MQ=255)
                                                                                              aaaGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGttt                                                    <  3:448631/149‑1 (MQ=255)
                                                                                                aGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGt                                                  <  2:25748/149‑1 (MQ=255)
                                                                                                 gCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTa                                                 <  3:239098/149‑1 (MQ=255)
                                                                                                            ggTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCg                                      >  1:609670/1‑149 (MQ=255)
                                                                                                              tttGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGcc                                    <  3:128877/149‑1 (MQ=255)
                                                                                                               ttGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCGGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCa                                   <  2:611709/149‑1 (MQ=255)
                                                                                                                       cAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGa                                                              <  4:127417/114‑1 (MQ=255)
                                                                                                                       cAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGa                                                              >  3:127417/1‑114 (MQ=255)
                                                                                                                           aCCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCaa                       >  4:230033/1‑149 (MQ=255)
                                                                                                                           aCCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCaa                       >  2:71040/1‑149 (MQ=255)
                                                                                                                              aacaaTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAgg                    >  4:650886/1‑149 (MQ=255)
                                                                                                                                 aaTGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCa                 >  2:492930/1‑149 (MQ=255)
                                                                                                                                   tGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCAcc               >  1:13171/1‑149 (MQ=255)
                                                                                                                                      ccGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACcgcg            >  2:173558/1‑149 (MQ=255)
                                                                                                                                       cgcgcgGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCgt           <  3:247751/149‑1 (MQ=255)
                                                                                                                                       cgcgcgGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCgt           <  2:547486/149‑1 (MQ=255)
                                                                                                                                                gATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCgg  >  2:574821/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGG  >  NC_000913/438780‑439072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑