| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,488,426 | A→T | 10.0% | intergenic (+26/+135) | lptG → / ← yjgR | lipopolysaccharide export ABC permease/DUF853 family protein with NTPase fold |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,488,426 | 0 | A | T | 10.0% | 319.8 / 21.0 | 99 | intergenic (+26/+135) | lptG/yjgR | lipopolysaccharide export ABC permease/DUF853 family protein with NTPase fold |
| Reads supporting (aligned to +/- strand): ref base A (44/45); new base T (3/7); total (47/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
ACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT > NC_000913/4488280‑4488570 | aCTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAg < 1:615013/149‑1 (MQ=255) ggACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTc < 2:669020/149‑1 (MQ=255) gACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTcc > 1:304724/1‑149 (MQ=255) ccAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCt > 1:267462/1‑149 (MQ=255) cAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCtt > 2:163924/1‑149 (MQ=255) cAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCtt > 1:685006/1‑149 (MQ=255) cAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCtt > 2:236852/1‑149 (MQ=255) cAGATCTGCGGCCCGCTGACGTTGGTTTATAGCAGCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGTGAAAGCTCCCtt < 3:51211/149‑1 (MQ=255) gATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTtta < 4:458135/149‑1 (MQ=255) gATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTtta > 2:396991/1‑149 (MQ=255) gATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAACTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTtta < 1:439386/149‑1 (MQ=255) aTCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTttat < 2:182447/149‑1 (MQ=255) tCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttatt < 3:439382/149‑1 (MQ=255) cTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttattc < 1:262520/149‑2 (MQ=255) cTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttattc < 2:349737/149‑2 (MQ=255) cTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttattc < 3:245670/149‑2 (MQ=255) ttCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTtattat > 1:563830/1‑149 (MQ=255) tCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTattatt < 1:236852/149‑1 (MQ=255) gCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCttt > 2:519238/1‑140 (MQ=255) gCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTATCGCTCCCttt < 1:519238/140‑1 (MQ=255) cccGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTa < 1:147370/149‑1 (MQ=255) cccGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTa < 2:491202/149‑1 (MQ=255) gTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAgtgt < 1:409222/149‑1 (MQ=255) gTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAgtgt > 1:330650/1‑149 (MQ=255) ttGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGtgtg > 3:313724/1‑149 (MQ=255) ggTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCt > 3:21112/1‑149 (MQ=255) ggTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCt < 4:123974/149‑1 (MQ=255) tttATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTc > 4:653174/1‑149 (MQ=255) tATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGt < 2:679834/149‑1 (MQ=255) aTGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTGCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGtt < 2:38861/149‑1 (MQ=255) tGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttattcgtt < 3:114922/128‑5 (MQ=39) tGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTttattcgtt > 4:114922/1‑124 (MQ=38) gCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTattatt < 3:317649/125‑1 (MQ=255) gCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTattatt > 4:317649/1‑125 (MQ=255) gCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCAt > 3:533172/1‑149 (MQ=255) cATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCAtt < 3:556511/149‑1 (MQ=255) aTCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTc > 1:635082/1‑149 (MQ=255) tCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTcc > 1:568435/1‑149 (MQ=255) tCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTcc > 3:648670/1‑149 (MQ=255) gccgATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGaa > 3:274039/1‑149 (MQ=255) catcGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAcataa < 2:635082/149‑1 (MQ=255) tcGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAAtt > 3:194744/1‑149 (MQ=255) tGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa < 2:176450/149‑1 (MQ=255) tGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa < 4:153377/149‑1 (MQ=255) ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAata > 1:693869/1‑149 (MQ=255) gCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAatata < 2:304724/149‑1 (MQ=255) cAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATAtt > 1:524828/1‑149 (MQ=255) aGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGc > 1:76626/1‑149 (MQ=255) cAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtgg < 3:666554/149‑1 (MQ=255) aGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggg < 2:582492/149‑1 (MQ=255) gCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtgggg > 3:288626/1‑149 (MQ=255) cttcttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggggt < 2:479696/149‑1 (MQ=255) ttcttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggggtg > 2:304477/1‑149 (MQ=255) tcttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggggtgg < 2:330650/149‑1 (MQ=255) tcttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggggtgg > 1:184389/1‑149 (MQ=255) ttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTa < 3:564939/85‑1 (MQ=255) ttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTa > 4:564939/1‑85 (MQ=255) ttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGc > 3:515219/1‑149 (MQ=255) ttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGc > 4:253000/1‑149 (MQ=255) ttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGc > 2:516112/1‑149 (MQ=255) cttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCAt < 2:267462/149‑1 (MQ=255) ttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATg > 1:190893/1‑149 (MQ=255) aTCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATa > 2:109753/1‑149 (MQ=255) aGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGa < 2:649065/149‑1 (MQ=255) cTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGAcgc < 1:121996/149‑1 (MQ=255) cTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGc > 4:343292/1‑149 (MQ=255) tGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGAc < 4:370961/140‑1 (MQ=255) tGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGAc > 3:370961/1‑140 (MQ=255) ttAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATAtt > 1:525755/1‑114 (MQ=255) ttAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATAtt < 2:525755/114‑1 (MQ=255) ttAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTc < 3:219864/149‑1 (MQ=255) ttAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTc < 2:76626/149‑1 (MQ=255) tAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCg < 3:570210/149‑1 (MQ=255) tGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCAt < 4:158857/149‑1 (MQ=255) gagaAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATc > 2:416342/1‑149 (MQ=255) gagaAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATc > 3:44332/1‑149 (MQ=255) agaAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCa < 2:524828/149‑1 (MQ=255) gaAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAg < 4:313724/149‑1 (MQ=255) aaaaTCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAgg < 2:243743/149‑1 (MQ=255) aaaaTCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAg < 2:58049/148‑1 (MQ=255) aaaaTCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAg > 1:58049/1‑148 (MQ=255) aaaTCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGc < 4:160745/149‑1 (MQ=255) tCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCaaa < 4:515219/149‑1 (MQ=255) aCCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGTTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACaa > 4:514795/1‑149 (MQ=255) gAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAAtc > 4:41619/1‑149 (MQ=255) tGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCg < 2:693869/149‑1 (MQ=255) gAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGc < 4:519936/149‑1 (MQ=255) gAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGc > 2:381445/1‑149 (MQ=255) aaCGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGcc > 4:44936/1‑149 (MQ=255) aaTAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGAc < 4:194744/149‑1 (MQ=255) tAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACtt > 1:62755/1‑149 (MQ=255) aaaGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGAc > 2:520595/1‑146 (MQ=255) aaaGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGAc < 1:520595/146‑1 (MQ=255) aGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTAcc > 2:550708/1‑149 (MQ=255) aGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTAcc > 1:96965/1‑149 (MQ=255) ggAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACctt > 1:434281/1‑149 (MQ=255) gAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACcttc < 3:69496/149‑1 (MQ=255) gCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACcttctt < 3:41619/149‑1 (MQ=255) gCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACcttctt < 2:96965/149‑1 (MQ=255) | ACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT > NC_000913/4488280‑4488570 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |