Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,561,587 G→T 6.3% A363A (GCC→GCA)  yjiJ ← DUF1228 family putative inner membrane MFS superfamily transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,561,5870GT 6.3% 266.9 / 5.2 77A363A (GCC→GCA) yjiJDUF1228 family putative inner membrane MFS superfamily transporter
Reads supporting (aligned to +/- strand):  ref base G (40/32);  new base T (3/2);  total (43/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

TCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGA  >  NC_000913/4561441‑4561728
                                                                                                                                                  |                                                                                                                                             
tCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGc                                                                                                                                             >  2:333184/1‑149 (MQ=255)
    gCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTTGCCAgc                                                                                                                                         >  1:333502/1‑149 (MQ=255)
      tGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAgcgc                                                                                                                                       >  2:360498/1‑149 (MQ=255)
      tGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAgcgc                                                                                                                                       >  2:492046/1‑149 (MQ=255)
      tGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGC‑TAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGcc                                                                                                                                      >  4:607056/1‑149 (MQ=255)
         cATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGa                                                                                                                                    <  4:591794/149‑1 (MQ=255)
          ataaaCGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCTAt                                                                                                                                   >  3:454113/4‑149 (MQ=255)
          aTCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTTGCCAGCGccact                                                                                                                                   <  2:333502/149‑4 (MQ=255)
           tCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATg                                                                                                                                  >  4:673979/1‑149 (MQ=255)
                   tgcggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTg                                                                                                                          <  3:538267/149‑1 (MQ=255)
                    gcggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGc                                                                                                                         >  2:264702/1‑149 (MQ=255)
                     cggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCt                                                                                                                        <  1:124244/149‑1 (MQ=255)
                     cggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCt                                                                                                                        <  3:380510/149‑1 (MQ=255)
                       gttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa                                                                                                                      >  2:665050/1‑149 (MQ=255)
                       gttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa                                                                                                                      <  2:447655/149‑1 (MQ=255)
                       gttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa                                                                                                                      <  1:266038/149‑1 (MQ=255)
                       gttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa                                                                                                                      <  4:510000/149‑1 (MQ=255)
                        ttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTg                                                                                                                                               <  1:198257/123‑1 (MQ=255)
                        ttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTg                                                                                                                                               >  2:198257/1‑123 (MQ=255)
                        ttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAg                                                                                                                     >  4:474386/1‑149 (MQ=255)
                        ttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAg                                                                                                                     <  3:295551/149‑1 (MQ=255)
                             tttCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATAc                                                                                                                >  2:140408/1‑149 (MQ=255)
                                  tGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTc                                                                                                           <  3:215054/149‑1 (MQ=255)
                                        gTAAGGGAAACTTCCCCCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGcc                                                                                                     >  1:212444/1‑149 (MQ=255)
                                             ggAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGcc                                                                                                >  2:221134/1‑149 (MQ=255)
                                                   ttCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGAt                                                                                          >  3:442139/1‑149 (MQ=255)
                                                   ttCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGAt                                                                                          <  1:360498/149‑1 (MQ=255)
                                                     cccTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGAttt                                                                                        <  2:635800/149‑1 (MQ=255)
                                                      ccTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTg                                                                                       >  3:577490/1‑149 (MQ=255)
                                                      ccTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTg                                                                                       >  1:539691/1‑149 (MQ=255)
                                                       cTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGGGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGc                                                                                      >  1:309640/1‑149 (MQ=255)
                                                       cTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGc                                                                                      <  3:219409/149‑1 (MQ=255)
                                                       cTCATGAAGTGACCACTAGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGACGATTTGc                                                                                      <  3:406098/149‑1 (MQ=255)
                                                        tCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGAGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGcc                                                                                     <  4:540386/149‑1 (MQ=255)
                                                           tGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCaa                                                                                  >  2:348767/1‑149 (MQ=255)
                                                               gTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGcc                                                                              >  1:663980/1‑149 (MQ=255)
                                                                tGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCg                                                                             >  1:661785/1‑149 (MQ=255)
                                                                   ccACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTaa                                                                          >  2:22819/1‑149 (MQ=255)
                                                                    cACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTaaa                                                                         >  4:387725/1‑149 (MQ=255)
                                                                       ttGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGa                                                                      >  1:381431/1‑149 (MQ=255)
                                                                         gTATCTTAAATAATTGCACTGTGCTGATAAGCGCATCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGc                                                                    <  1:647517/149‑1 (MQ=255)
                                                                              ttAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTcac                                                               >  2:497857/1‑149 (MQ=255)
                                                                                 aataatTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCAaaaa                                                            <  4:21041/149‑1 (MQ=255)
                                                                                 aataatTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATCAGCGTCACaaa                                                            >  3:443669/1‑149 (MQ=255)
                                                                                   taatTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGc                                                          <  2:161285/149‑1 (MQ=255)
                                                                                   taatTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGc                                                          <  1:345234/149‑1 (MQ=255)
                                                                                     atTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATCCTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCt                                                        <  4:471286/149‑1 (MQ=255)
                                                                                     atTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGcca                                                        <  2:209516/149‑2 (MQ=255)
                                                                                           cTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGa                                                  <  3:383345/149‑1 (MQ=255)
                                                                                             gtgCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGAtt                                                <  3:507186/149‑1 (MQ=255)
                                                                                                cTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTgag                                             <  4:597078/149‑1 (MQ=255)
                                                                                                  gATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGAt                                           >  1:240496/1‑149 (MQ=255)
                                                                                                      aGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTc                                        <  4:420072/148‑1 (MQ=255)
                                                                                                      aGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTc                                        >  3:420072/1‑148 (MQ=255)
                                                                                                        cgcAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCg                                     <  2:661785/149‑1 (MQ=255)
                                                                                                         gcagcaATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCg                                                                                                    <  3:668477/85‑1 (MQ=255)
                                                                                                         gcagcaATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCg                                                                                                    >  4:668477/1‑85 (MQ=255)
                                                                                                         gcagcaATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGCGGATTTGCCCAATGCCGTAAATGAGCGCCACATAGCTGACACGATTGAGATTTCCCgg                                    >  4:469511/1‑149 (MQ=255)
                                                                                                           agcaATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTa                                  >  4:339337/1‑149 (MQ=255)
                                                                                                              aaTAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGc                               >  2:601178/1‑149 (MQ=255)
                                                                                                               aTAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCt                              >  2:118714/1‑149 (MQ=255)
                                                                                                               aTAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCt                              >  2:117001/1‑149 (MQ=255)
                                                                                                               aTAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCt                              >  1:371730/1‑149 (MQ=255)
                                                                                                                 aaaCAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCa                            <  2:395093/149‑1 (MQ=255)
                                                                                                                  aacaacGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAg                           >  2:175747/1‑149 (MQ=255)
                                                                                                                     aacGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTg                        >  2:491671/1‑149 (MQ=255)
                                                                                                                     aacGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTg                        >  3:176702/1‑149 (MQ=255)
                                                                                                                      acGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTgg                       >  3:439520/1‑149 (MQ=255)
                                                                                                                          cgccgcgccgCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTggcggg                   >  1:264667/1‑149 (MQ=255)
                                                                                                                             cgcgccgcAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACGGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGATATTTCCCGGTACGCTCAGTTGGCGGGCGa                <  4:454113/149‑1 (MQ=255)
                                                                                                                                  cgcAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGtc           <  1:665050/149‑1 (MQ=255)
                                                                                                                                  cgcAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGtc           <  3:474386/149‑1 (MQ=255)
                                                                                                                                     agagagTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGtcatc        <  1:252052/149‑1 (MQ=255)
                                                                                                                                      gagagTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGtcatca       >  2:511232/1‑149 (MQ=255)
                                                                                                                                         atcggCGCTTGCCAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCa    >  3:679586/4‑149 (MQ=255)
                                                                                                                                         agTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCa    >  3:343861/1‑149 (MQ=255)
                                                                                                                                          tcggCGCTTGCCAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGa                                                                                              <  3:213586/56‑1 (MQ=255)
                                                                                                                                          tcggCGCTTGCCAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGa                                                                                              >  4:213586/3‑58 (MQ=255)
                                                                                                                                           tGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCaga  <  2:582793/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
TCTGGCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGA  >  NC_000913/4561441‑4561728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑