Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,561,590 A→C 6.5% L362L (CTT→CTG yjiJ ← DUF1228 family putative inner membrane MFS superfamily transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,561,5900AC 6.5% 261.8 / 8.6 77L362L (CTT→CTGyjiJDUF1228 family putative inner membrane MFS superfamily transporter
Reads supporting (aligned to +/- strand):  ref base A (39/33);  new base C (3/2);  total (42/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGC  >  NC_000913/4561445‑4561738
                                                                                                                                                 |                                                                                                                                                    
gCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTTGCCAgc                                                                                                                                                   >  1:333502/1‑149 (MQ=255)
  tGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAgcgc                                                                                                                                                 >  2:492046/1‑149 (MQ=255)
  tGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAgcgc                                                                                                                                                 >  2:360498/1‑149 (MQ=255)
  tGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGcc                                                                                                                                                >  4:607056/1‑149 (MQ=255)
     cATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGa                                                                                                                                              <  4:591794/149‑1 (MQ=255)
      ataaaCGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCTAt                                                                                                                                             >  3:454113/4‑149 (MQ=255)
      aTCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTTGCCAGCGccact                                                                                                                                             <  2:333502/149‑4 (MQ=255)
       tCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATg                                                                                                                                            >  4:673979/1‑149 (MQ=255)
               tgcggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTg                                                                                                                                    <  3:538267/149‑1 (MQ=255)
                gcggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGc                                                                                                                                   >  2:264702/1‑149 (MQ=255)
                 cggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCt                                                                                                                                  <  3:380510/149‑1 (MQ=255)
                 cggttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCt                                                                                                                                  <  1:124244/149‑1 (MQ=255)
                   gttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa                                                                                                                                >  2:665050/1‑149 (MQ=255)
                   gttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa                                                                                                                                <  2:447655/149‑1 (MQ=255)
                   gttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa                                                                                                                                <  4:510000/149‑1 (MQ=255)
                   gttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGa                                                                                                                                <  1:266038/149‑1 (MQ=255)
                    ttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAg                                                                                                                               <  3:295551/149‑1 (MQ=255)
                    ttgAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAg                                                                                                                               >  4:474386/1‑149 (MQ=255)
                         tttCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATAc                                                                                                                          >  2:140408/1‑149 (MQ=255)
                              tGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTc                                                                                                                     <  3:215054/149‑1 (MQ=255)
                                    gTAAGGGAAACTTCCCCCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGcc                                                                                                               >  1:212444/1‑149 (MQ=255)
                                         ggAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGcc                                                                                                          >  2:221134/1‑149 (MQ=255)
                                               ttCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGAt                                                                                                    <  1:360498/149‑1 (MQ=255)
                                               ttCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGAt                                                                                                    >  3:442139/1‑149 (MQ=255)
                                                 cccTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGAttt                                                                                                  <  2:635800/149‑1 (MQ=255)
                                                  ccTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTg                                                                                                 >  1:539691/1‑149 (MQ=255)
                                                  ccTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTg                                                                                                 >  3:577490/1‑149 (MQ=255)
                                                   cTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGGGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGc                                                                                                >  1:309640/1‑149 (MQ=255)
                                                   cTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGc                                                                                                <  3:219409/149‑1 (MQ=255)
                                                   cTCATGAAGTGACCACTAGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGACGATTTGc                                                                                                <  3:406098/149‑1 (MQ=255)
                                                    tCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGAGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGcc                                                                                               <  4:540386/149‑1 (MQ=255)
                                                       tGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCaa                                                                                            >  2:348767/1‑149 (MQ=255)
                                                           gTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGcc                                                                                        >  1:663980/1‑149 (MQ=255)
                                                            tGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCg                                                                                       >  1:661785/1‑149 (MQ=255)
                                                               ccACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTaa                                                                                    >  2:22819/1‑149 (MQ=255)
                                                                cACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTaaa                                                                                   >  4:387725/1‑149 (MQ=255)
                                                                   ttGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGa                                                                                >  1:381431/1‑149 (MQ=255)
                                                                     gTATCTTAAATAATTGCACTGTGCTGATAAGCGCATCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGc                                                                              <  1:647517/149‑1 (MQ=255)
                                                                          ttAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTcac                                                                         >  2:497857/1‑149 (MQ=255)
                                                                             aataatTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCAaaaa                                                                      <  4:21041/149‑1 (MQ=255)
                                                                             aataatTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATCAGCGTCACaaa                                                                      >  3:443669/1‑149 (MQ=255)
                                                                               taatTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGc                                                                    <  1:345234/149‑1 (MQ=255)
                                                                               taatTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGc                                                                    <  2:161285/149‑1 (MQ=255)
                                                                                 atTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATCCTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCt                                                                  <  4:471286/149‑1 (MQ=255)
                                                                                 atTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGcca                                                                  <  2:209516/149‑2 (MQ=255)
                                                                                       cTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGa                                                            <  3:383345/149‑1 (MQ=255)
                                                                                         gtgCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGAtt                                                          <  3:507186/149‑1 (MQ=255)
                                                                                            cTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTgag                                                       <  4:597078/149‑1 (MQ=255)
                                                                                              gATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGAt                                                     >  1:240496/1‑149 (MQ=255)
                                                                                                  aGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTc                                                  >  3:420072/1‑148 (MQ=255)
                                                                                                  aGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTc                                                  <  4:420072/148‑1 (MQ=255)
                                                                                                    cgcAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCg                                               <  2:661785/149‑1 (MQ=255)
                                                                                                     gcagcaATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCg                                                                                                              <  3:668477/85‑1 (MQ=255)
                                                                                                     gcagcaATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCg                                                                                                              >  4:668477/1‑85 (MQ=255)
                                                                                                     gcagcaATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGCGGATTTGCCCAATGCCGTAAATGAGCGCCACATAGCTGACACGATTGAGATTTCCCgg                                              >  4:469511/1‑149 (MQ=255)
                                                                                                       agcaATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTa                                            >  4:339337/1‑149 (MQ=255)
                                                                                                          aaTAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGc                                         >  2:601178/1‑149 (MQ=255)
                                                                                                           aTAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCt                                        >  1:371730/1‑149 (MQ=255)
                                                                                                           aTAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCt                                        >  2:117001/1‑149 (MQ=255)
                                                                                                           aTAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCt                                        >  2:118714/1‑149 (MQ=255)
                                                                                                             aaaCAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCa                                      <  2:395093/149‑1 (MQ=255)
                                                                                                              aacaacGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAg                                     >  2:175747/1‑149 (MQ=255)
                                                                                                                 aacGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTg                                  >  3:176702/1‑149 (MQ=255)
                                                                                                                 aacGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTg                                  >  2:491671/1‑149 (MQ=255)
                                                                                                                  acGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTgg                                 >  3:439520/1‑149 (MQ=255)
                                                                                                                      cgccgcgccgCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTggcggg                             >  1:264667/1‑149 (MQ=255)
                                                                                                                         cgcgccgcAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACGGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGATATTTCCCGGTACGCTCAGTTGGCGGGCGa                          <  4:454113/149‑1 (MQ=255)
                                                                                                                              cgcAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGtc                     <  3:474386/149‑1 (MQ=255)
                                                                                                                              cgcAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGtc                     <  1:665050/149‑1 (MQ=255)
                                                                                                                                 agagagTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGtcatc                  <  1:252052/149‑1 (MQ=255)
                                                                                                                                  gagagTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGtcatca                 >  2:511232/1‑149 (MQ=255)
                                                                                                                                     atcggCGCTTGCCAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCa              >  3:679586/4‑149 (MQ=255)
                                                                                                                                     agTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCa              >  3:343861/1‑149 (MQ=255)
                                                                                                                                      tcggCGCTTGCCAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGa                                                                                                        >  4:213586/3‑58 (MQ=255)
                                                                                                                                      tcggCGCTTGCCAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGa                                                                                                        <  3:213586/56‑1 (MQ=255)
                                                                                                                                       tGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCaga            <  2:582793/149‑1 (MQ=255)
                                                                                                                                               gCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGt    <  2:290233/149‑1 (MQ=255)
                                                                                                                                                 aaGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGc  <  1:140408/149‑1 (MQ=255)
                                                                                                                                                 aaGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGAGACGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGc  <  1:363673/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCTGACATCAACGGTTGCGGTTGAGTTTCTTGAGTGGTAAGGGAAACTTCCCTCATGAAGTGACCACTTGTAGCTTAAATAATTGCACTGTGCTGATAAGCGCAGCAATAAACAACGCCGCCGCGCCGCAGAGAGTGGCGCTGGCAAGCGCCGATGTTCCATTGCTGAGCATACTGGTCAGCGCCGGGCCGAGGATTTGCCCAATGCCGTAAATGAGCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGC  >  NC_000913/4561445‑4561738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: