Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,573,189 T→C 6.9% pseudogene (776/1503 nt) yjiT → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,573,1890TC 6.9% 319.4 / 11.1 102pseudogene (776/1503 nt)yjiTpseudogene
Reads supporting (aligned to +/- strand):  ref base T (47/48);  new base C (4/3);  total (51/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01

Only 100 of 109 total aligned reads displayed.

TTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTACGCGCTT  >  NC_000913/4573041‑4573336
                                                                                                                                                     |                                                                                                                                                   
ttAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                                                                     <  2:701358/149‑1 (MQ=255)
ttAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                                                                     >  1:138936/1‑149 (MQ=255)
ttAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                                                                     <  2:18309/149‑1 (MQ=255)
ttAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                                                                     >  3:559627/1‑149 (MQ=255)
ttAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGt                                                                                                                                                     <  3:24484/149‑1 (MQ=255)
   gTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTaga                                                                                                                                                  <  1:687701/149‑1 (MQ=255)
   gTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTaga                                                                                                                                                  <  1:242936/149‑1 (MQ=255)
   gTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTaga                                                                                                                                                  <  1:534878/149‑1 (MQ=255)
   gTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTaga                                                                                                                                                  >  3:42093/1‑149 (MQ=255)
   gTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTAGAATGGACTGCTATGCGCAGCCTCTGTaga                                                                                                                                                  >  1:453962/1‑149 (MQ=255)
    tacttactCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTagag                                                                                                                                                 <  2:272932/149‑1 (MQ=255)
       ttactCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTATATGATGAAACAGGTACGCATTTTTTTTAATGGAGTGCTATGGGCAGTCCCTGTAGAGGc                                                                                                                                               >  2:702411/1‑149 (MQ=255)
         actCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGATTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTaa                                                                                                                                            <  2:567960/149‑1 (MQ=255)
          ctCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAg                                                                                                                                           <  2:32134/149‑1 (MQ=255)
           tCACAACTTAATAAATCACAAAGAACCAGTACAAAAGCGTGAGAAGGTTAACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATTATGAAACACGAACGCATTTTTTGAAGGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGc                                                                                                                                          >  2:493306/1‑149 (MQ=255)
           tCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGc                                                                                                                                          >  3:239766/1‑149 (MQ=255)
           tCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGc                                                                                                                                          >  2:544776/1‑149 (MQ=255)
           tCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTACAGAGGCtgcgc                                                                                                                                          >  1:70924/1‑145 (MQ=255)
            cacaACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTACAGAGGCtgcgca                                                                                                                                         <  1:46526/149‑6 (MQ=255)
            cacaACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTACAGAGGCtgcgca                                                                                                                                         <  3:192264/149‑6 (MQ=255)
             acaACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCt                                                                                                                                        >  1:192543/1‑149 (MQ=255)
             acaACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGCTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCt                                                                                                                                        >  4:336174/1‑149 (MQ=255)
               aaCCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCg                                                                                                                                      >  1:367545/1‑149 (MQ=255)
               aaCCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCg                                                                                                                                      >  2:34407/1‑149 (MQ=255)
               aaCCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCg                                                                                                                                      >  1:568070/1‑149 (MQ=255)
                aCCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGTCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTGGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGa                                                                                                                                     >  4:572719/1‑149 (MQ=255)
                 ccTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGAc                                                                                                                                    >  3:662587/1‑149 (MQ=255)
                   taataaATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTa                                                                                                                                  >  4:324049/1‑149 (MQ=255)
                   taataaATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTa                                                                                                                                  >  2:120213/1‑149 (MQ=255)
                   taataaATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTa                                                                                                                                  >  3:180674/1‑149 (MQ=255)
                      taaATCACAAAGAACCAGTACAACAGCTTGAGAACGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGACGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACaa                                                                                                                               >  2:479838/1‑149 (MQ=255)
                              aaaGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATaa                                                                                                                       >  2:186035/1‑149 (MQ=255)
                              aaaGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATaa                                                                                                                       >  3:81556/1‑149 (MQ=255)
                              aaaGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATaa                                                                                                                       <  4:662587/149‑1 (MQ=255)
                                  aaCCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGt                                                                                                                   >  4:208065/1‑149 (MQ=255)
                                    ccAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTAc                                                                                                                 <  4:532108/149‑1 (MQ=255)
                                    ccAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTAc                                                                                                                 <  1:328196/149‑1 (MQ=255)
                                           aacaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAaga                                                                                                                     >  2:119860/1‑138 (MQ=255)
                                           aacaGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAaga                                                                                                                     <  1:119860/138‑1 (MQ=255)
                                              aGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCaa                                                                                                       >  3:529406/1‑149 (MQ=255)
                                              aGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCaa                                                                                                       >  1:242120/1‑149 (MQ=255)
                                                cTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAAtt                                                                                                     >  1:426680/1‑149 (MQ=255)
                                                cTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAAtt                                                                                                     <  2:419487/149‑1 (MQ=255)
                                                  tGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCt                                                                                                   <  1:105333/149‑1 (MQ=255)
                                                     gaAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTAtt                                                                                                <  2:511546/149‑1 (MQ=255)
                                                     gaAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTAtt                                                                                                <  4:126010/149‑1 (MQ=255)
                                                         gTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCATCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTc                                                                                            <  1:693643/149‑1 (MQ=255)
                                                          ttCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCa                                                                                           <  3:351286/149‑1 (MQ=255)
                                                          ttCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCa                                                                                           <  4:191886/149‑1 (MQ=255)
                                                              cccTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGaaa                                                                                       >  2:444242/1‑149 (MQ=255)
                                                               ccTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTa                                                                                                                  <  3:600164/121‑1 (MQ=255)
                                                               ccTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTa                                                                                                                  >  4:600164/1‑121 (MQ=255)
                                                               ccTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGaaaa                                                                                      >  2:396399/1‑149 (MQ=255)
                                                               ccTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGaaaa                                                                                      <  2:453962/149‑1 (MQ=255)
                                                               ccTACCTGGCGAAGCGAGATTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGaaaa                                                                                      <  2:296764/149‑1 (MQ=255)
                                                                 tACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACCGGTACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGATAAGc                                                                                    >  4:371495/1‑149 (MQ=255)
                                                                    cTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATc                                                                                 <  4:488596/149‑1 (MQ=255)
                                                                     tGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATcc                                                                                <  3:18455/149‑1 (MQ=255)
                                                                      ggCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCa                                                                               >  1:19195/1‑149 (MQ=255)
                                                                        cGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGa                                                                             <  2:169089/149‑1 (MQ=255)
                                                                         gAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGAt                                                                            >  1:106911/1‑149 (MQ=255)
                                                                           aGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGa                                                                          <  2:192543/149‑1 (MQ=255)
                                                                           aGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGa                                                                          <  3:600950/149‑1 (MQ=255)
                                                                               aGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATaa                                                                                                                       >  4:604830/1‑100 (MQ=255)
                                                                               aGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATaa                                                                                                                       <  3:604830/100‑1 (MQ=255)
                                                                                    ccAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGt                                                                 <  4:42093/149‑1 (MQ=255)
                                                                                      aaTACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGa                                                               >  3:106582/1‑149 (MQ=255)
                                                                                      aaTACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGa                                                               >  2:542737/1‑149 (MQ=255)
                                                                                        tACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCGAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAATAGCATCCAGATGAGCTTAGGGTGATa                                                             <  4:582792/149‑1 (MQ=255)
                                                                                             ttAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTAcgc                                                                                                               >  4:456064/1‑94 (MQ=255)
                                                                                                       aaaCAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGAc                                              <  3:41644/149‑1 (MQ=255)
                                                                                                       aaaCAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGAc                                              >  3:131314/1‑149 (MQ=255)
                                                                                                        aaCAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACg                                             >  1:605866/1‑149 (MQ=255)
                                                                                                         aCAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGa                                            >  2:11275/1‑149 (MQ=255)
                                                                                                              aaCGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGttt                                       <  3:6741/149‑1 (MQ=255)
                                                                                                               aCGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttc                                      <  4:180674/149‑1 (MQ=255)
                                                                                                                 gCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcct                                    <  2:614891/149‑1 (MQ=255)
                                                                                                                 gCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcct                                    >  1:430274/1‑149 (MQ=255)
                                                                                                                     ttttttGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcctttc                                 <  1:38519/149‑1 (MQ=255)
                                                                                                                     ttttttGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcctttc                                 <  1:444242/149‑1 (MQ=255)
                                                                                                                     ttttttGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcctttc                                 <  1:396399/149‑1 (MQ=255)
                                                                                                                           gAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTCGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTaa                           >  1:289792/1‑149 (MQ=255)
                                                                                                                           gAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTaa                           >  2:285064/1‑149 (MQ=255)
                                                                                                                             aTGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACa                         >  4:485739/1‑149 (MQ=255)
                                                                                                                              tGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACaa                        <  4:554680/149‑1 (MQ=255)
                                                                                                                                gACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGc                      <  2:106911/149‑1 (MQ=255)
                                                                                                                                 aCTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCg                     <  2:452188/149‑1 (MQ=255)
                                                                                                                                       aTGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcc                                     >  3:351104/1‑127 (MQ=255)
                                                                                                                                       aTGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcc                                     <  4:389014/127‑1 (MQ=255)
                                                                                                                                       aTGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcc                                     <  4:351104/127‑1 (MQ=255)
                                                                                                                                       aTGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGtttcc                                     >  3:389014/1‑127 (MQ=255)
                                                                                                                                         gcttaGCCTCTACAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCa             >  3:116558/5‑149 (MQ=255)
                                                                                                                                          cttaGCCTCTACAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTAtctc                                                      >  3:174301/4‑107 (MQ=255)
                                                                                                                                           ttaGCCTCTACAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAAc           >  4:573155/3‑149 (MQ=255)
                                                                                                                                              gCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTAc        <  3:517105/149‑1 (MQ=255)
                                                                                                                                                ctctGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTAcgc      >  4:318771/1‑149 (MQ=255)
                                                                                                                                                 tctGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTAcgcg     >  1:552612/1‑149 (MQ=255)
                                                                                                                                                    gTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTACGCGCtt  <  1:257761/149‑1 (MQ=255)
                                                                                                                                                     tAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCaa            <  4:481928/138‑1 (MQ=255)
                                                                                                                                                     tAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCaa            >  3:481928/1‑138 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTAGTACTTACTCACAACCTAATAAATCACAAAGAACCAGTACAACAGCTTGAGAAGGTTCACCCTACCTGGCGAAGCGAGTTTCCAATACCGTTAGATGATGAAACAGGAACGCATTTTTTGAATGGACTGCTATGCGCAGCCTCTGTAGAGGCTAAGCCTCGACTACAAAAAAATAAGAGTACGCGTTGCCAATTCTATTGGTCAGAAAAGCATCCAGATGAGCTTAGGGTGATAGTATCTCTTCCGGACGAAGTTTCCTTTCCTGTAACAAGCGAGCCGTCAACTACGCGCTT  >  NC_000913/4573041‑4573336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: