Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,637,492 A→T 5.9% E266V (GAG→GTG)  creC → sensory histidine kinase in two‑component regulatory system with CreB or PhoB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,637,4920AT 5.9% 273.6 / 5.9 81E266V (GAG→GTG) creCsensory histidine kinase in two‑component regulatory system with CreB or PhoB
Reads supporting (aligned to +/- strand):  ref base A (41/35);  new base T (2/3);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCC  >  NC_000913/4637344‑4637635
                                                                                                                                                    |                                                                                                                                               
gCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGa                                                                                                                                                 <  2:255407/149‑1 (MQ=255)
gCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGa                                                                                                                                                 <  4:68504/149‑1 (MQ=255)
          tCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGTAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGc                                                                                                                                       >  2:578735/1‑149 (MQ=255)
          tCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGc                                                                                                                                       >  4:486024/1‑149 (MQ=255)
             cTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCa                                                                                                                                    >  4:94472/1‑149 (MQ=255)
             cTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCa                                                                                                                                    <  4:260911/149‑1 (MQ=255)
             cTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCa                                                                                                                                    <  4:102126/149‑1 (MQ=255)
              tGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCAc                                                                                                                                   >  2:221576/1‑149 (MQ=255)
              tGACAATAAGCCCGTTCCTCTCCCAGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCAc                                                                                                                                   >  3:380552/1‑149 (MQ=255)
               gACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACt                                                                                                                                  >  3:678444/1‑149 (MQ=255)
               gACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTg                                                                                                                                 >  2:452903/1‑149 (MQ=255)
                aCAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGTGCTAAAAAGCCCACTg                                                                                                                                 >  3:311748/1‑149 (MQ=255)
                aCAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGTGCTAAAAAGCCCACTg                                                                                                                                 >  3:502901/1‑149 (MQ=255)
                 cAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTgg                                                                                                                                <  2:576334/149‑1 (MQ=255)
                   aTAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTggcg                                                                                                                              >  4:370915/1‑149 (MQ=255)
                           gTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCg                                                                                                                      >  3:306917/1‑149 (MQ=255)
                            ttCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGt                                                                                                                     <  1:565598/149‑1 (MQ=255)
                             tCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTg                                                                                                                    <  2:531912/149‑1 (MQ=255)
                             tCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTg                                                                                                                    <  1:226323/149‑1 (MQ=255)
                                   ccccGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAgcgg                                                                                                              >  1:432109/1‑149 (MQ=255)
                                   ccccGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAgcgg                                                                                                              >  1:685777/1‑149 (MQ=255)
                                   ccccGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAgcgg                                                                                                              <  1:71408/149‑1 (MQ=255)
                                    cccGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAgcggc                                                                                                             <  3:281862/149‑1 (MQ=255)
                                    cccGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAgcggc                                                                                                             >  1:75317/1‑149 (MQ=255)
                                    cccGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAgcggc                                                                                                             <  4:402675/149‑1 (MQ=255)
                                    cccGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAgcggc                                                                                                             >  1:262692/1‑149 (MQ=255)
                                     ccGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAgcggcg                                                                                                            >  4:626246/1‑149 (MQ=255)
                                        aTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGaa                                                                                                         >  2:213519/1‑149 (MQ=255)
                                          ctcGGTAGTAGCGAGTTGCGTAAGCTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAAt                                                                                                       <  4:326583/149‑1 (MQ=255)
                                          ctcGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAAt                                                                                                       <  4:678444/149‑1 (MQ=255)
                                           tcGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAAtt                                                                                                      >  2:269782/1‑149 (MQ=255)
                                           tcGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAAtt                                                                                                      >  2:610043/1‑149 (MQ=255)
                                           tcGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAAtt                                                                                                      >  2:492114/1‑149 (MQ=255)
                                                     cGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAg                                                                                            <  2:193764/149‑1 (MQ=255)
                                                           gCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTccgc                                                                                      >  1:499773/1‑149 (MQ=255)
                                                            cGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTccgcc                                                                                     <  1:277472/149‑1 (MQ=255)
                                                             gTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTccgccg                                                                                    >  4:328163/1‑149 (MQ=255)
                                                              tAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTccgccgc                                                                                   >  4:601447/1‑149 (MQ=255)
                                                              tAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTccgccgc                                                                                   >  1:189885/1‑149 (MQ=255)
                                                                   tCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGaa                                                                              <  3:328163/149‑1 (MQ=255)
                                                                   tCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGaa                                                                              <  4:396286/149‑1 (MQ=255)
                                                                   tCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGaa                                                                              >  1:142093/1‑149 (MQ=255)
                                                                     gcgcAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAgt                                                                            >  3:606182/1‑149 (MQ=255)
                                                                        cAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAgtggt                                                                         <  4:163357/149‑1 (MQ=255)
                                                                        cAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAgtggt                                                                         <  3:486024/149‑1 (MQ=255)
                                                                               tGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGt                                                                  >  1:134969/1‑149 (MQ=255)
                                                                                ggAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGtt                                                                 <  2:325392/149‑1 (MQ=255)
                                                                                 gAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGttt                                                                >  3:37180/1‑149 (MQ=255)
                                                                                   aaGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTa                                                              <  4:380552/149‑1 (MQ=255)
                                                                                    aGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTAc                                                             <  3:632832/149‑1 (MQ=255)
                                                                                    aGTATGCAAATGAAACTGGAAGGGAAAAACTATATTGAGCAGAATGATTTCGCCATAACTCATGTGATAAAAAGCCCCCTGGCGGCGATTCTTGGGGCCGTGGAAAGTTTGCGCGAAGGCCCGCCGCCGGACGTGGTGGCACGTTTTAc                                                             <  4:32595/149‑1 (MQ=255)
                                                                                       aTGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGa                                                          <  2:685777/149‑1 (MQ=255)
                                                                                              tGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACAtt                                                   <  1:662365/149‑1 (MQ=255)
                                                                                                   cTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGAc                                              <  2:189885/149‑1 (MQ=255)
                                                                                                   cTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGAc                                              <  1:476082/149‑1 (MQ=255)
                                                                                                      gAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCa                                           >  4:39192/1‑149 (MQ=255)
                                                                                                        aGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCaaa                                         >  4:878/1‑149 (MQ=255)
                                                                                                           gAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATg                                      >  1:623814/1‑149 (MQ=255)
                                                                                                              aaaCTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATgcgc                                   <  2:414986/149‑1 (MQ=255)
                                                                                                                aCTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGt                                 >  3:439974/1‑149 (MQ=255)
                                                                                                                  tataTTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTAt                               >  3:138293/1‑149 (MQ=255)
                                                                                                                  tataTTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTAt                               >  2:423102/1‑149 (MQ=255)
                                                                                                                      ttGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAg                           <  3:370915/149‑1 (MQ=255)
                                                                                                                      ttGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAg                           <  2:499773/149‑1 (MQ=255)
                                                                                                                      ttGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAg                           >  1:411851/1‑149 (MQ=255)
                                                                                                                       tGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAgg                          <  2:563446/149‑1 (MQ=255)
                                                                                                                         aGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCa                        >  4:641994/1‑149 (MQ=255)
                                                                                                                           cAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCAtt                      <  1:83343/149‑1 (MQ=255)
                                                                                                                            aGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTg                     <  2:486553/149‑1 (MQ=255)
                                                                                                                            aGTAAGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGAGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGCTGTACTGTCAACATTCTGACGCAAAATGCGCGTATGCAGGCATTg                     >  4:324300/1‑149 (MQ=255)
                                                                                                                                    tACGCATTAACTCATGTGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAAc             <  4:311748/149‑1 (MQ=255)
                                                                                                                                    tACGCATTAACTCATGTGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAAGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAAc             <  4:502901/149‑1 (MQ=255)
                                                                                                                                    tACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAAc             <  2:623814/149‑1 (MQ=255)
                                                                                                                                       gCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACACAAAATGCGCGTATGCAGGCATTGGTAGAAACGtt          >  4:575197/1‑149 (MQ=255)
                                                                                                                                        cATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAAc             >  1:257096/1‑145 (MQ=255)
                                                                                                                                        cATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAAc             <  2:257096/145‑1 (MQ=255)
                                                                                                                                        cATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTta         <  4:439974/149‑1 (MQ=255)
                                                                                                                                         aTTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTtac        >  1:550783/1‑149 (MQ=255)
                                                                                                                                         aTTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTtac        >  1:259074/1‑149 (MQ=255)
                                                                                                                                              cTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGTAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGc   <  3:446088/149‑1 (MQ=255)
                                                                                                                                               tCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGcc  >  4:49130/1‑149 (MQ=255)
                                                                                                                                                 aTGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTAc                                                             >  1:507340/1‑88 (MQ=255)
                                                                                                                                                 aTGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTAc                                                             <  2:507340/88‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCC  >  NC_000913/4637344‑4637635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: