Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 824,572 T→A 7.0% intergenic (‑75/‑58) ybhP ← / → ybhQ endo/exonuclease/phosphatase family protein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913824,5720TA 7.0% 236.0 / 6.5 71intergenic (‑75/‑58)ybhP/ybhQendo/exonuclease/phosphatase family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (30/36);  new base A (2/3);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

AACGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGG  >  NC_000913/824427‑824717
                                                                                                                                                 |                                                                                                                                                 
aaCGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAtttt                                                                                                                                                >  4:198864/1‑149 (MQ=255)
  cgcgGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTCGGCGACGTCACAAAAAGGTGCGGTCTTCCGGCAtccgcc                                                                                                                                              <  2:489433/149‑6 (MQ=255)
   gcgGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCg                                                                                                                                             >  1:153644/1‑149 (MQ=255)
       tAAAGCCTTTGTGAATATTGGTGGTGAGCTCCTTGAACGAAAATCGTTGTGTTTGTTCGGGCATGATTTTCCTGTTTTTGCCACTTATCTCATTGAAATAGTGTAGTCGGCGTCCCAGAAAGGTGCGGTCTTACGGAATTTTCCGTaaa                                                                                                                                         <  2:27789/149‑1 (MQ=255)
        aaaGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAg                                                                                                                                        <  3:182812/149‑1 (MQ=255)
         aaGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaagac                                                                                                                                       <  4:619510/149‑4 (MQ=255)
              tttGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaaa                                                                                                                                         <  2:699831/142‑1 (MQ=255)
              tttGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaaa                                                                                                                                         >  1:699831/1‑142 (MQ=255)
              tttGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGt                                                                                                                                  >  1:115250/1‑149 (MQ=255)
              tttGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGt                                                                                                                                  >  1:198679/1‑149 (MQ=255)
                   gAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActct                                                                                                                             <  1:655586/149‑1 (MQ=255)
                     atatTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGa                                                                                                                           <  1:202685/149‑1 (MQ=255)
                     atatTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGa                                                                                                                           <  2:198679/149‑1 (MQ=255)
                      tatTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGag                                                                                                                          <  2:558775/149‑1 (MQ=255)
                      tatTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGAGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGag                                                                                                                          <  2:359404/149‑1 (MQ=255)
                       atTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagt                                                                                                                         >  2:142027/1‑149 (MQ=255)
                            tGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaagta                                                                                                                    <  3:127919/149‑1 (MQ=255)
                              gTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTaga                                                                                                                  <  4:122907/149‑1 (MQ=255)
                                      cTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaaga                                                                                                                                        <  3:406686/119‑3 (MQ=255)
                                      cTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaaga                                                                                                                                        >  4:406686/1‑117 (MQ=255)
                                          aaCGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAttcttc                                                                                                      <  2:374216/149‑1 (MQ=255)
                                           tctaaaaTTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTTTGTATAGGCAAATTCTTCa                                                                                                     <  4:274028/146‑1 (MQ=255)
                                             gAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAgg                                                                                                   >  1:584953/1‑149 (MQ=255)
                                             gAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTATTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAgg                                                                                                   <  3:439744/149‑1 (MQ=255)
                                              aaaaTTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGga                                                                                                  <  2:599381/149‑1 (MQ=255)
                                              aaaaTTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGga                                                                                                  <  2:584953/149‑1 (MQ=255)
                                               aaaTTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaa                                                                                                                       <  4:371562/127‑1 (MQ=255)
                                               aaaTTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaa                                                                                                                       >  3:371562/1‑127 (MQ=255)
                                                aaTTGTTGTGTTTGATCGGGCATTATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGgaga                                                                                                >  3:229041/1‑149 (MQ=255)
                                                     ttgtGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGc                                                                                           <  3:198864/149‑1 (MQ=255)
                                                     ttgtGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGc                                                                                           >  1:681424/1‑149 (MQ=255)
                                                          tttGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGa                                                                                      >  3:19/1‑149 (MQ=255)
                                                             gATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGt                                                                                   <  1:263001/149‑1 (MQ=255)
                                                                  ggCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGCAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGcaa                                                                              <  4:598015/149‑1 (MQ=255)
                                                                     aTAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGcaacaa                                                                           <  1:142027/149‑1 (MQ=255)
                                                                        aTTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACgt                                                                        <  4:229041/149‑1 (MQ=255)
                                                                         ttttCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACgtg                                                                       >  1:626699/1‑149 (MQ=255)
                                                                                gTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCgtgt                                                                >  1:1497/1‑149 (MQ=255)
                                                                                   tttGCCTCTTATCTCATTGAAATAGTGTAGTAGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGc                                                             >  1:674413/1‑149 (MQ=255)
                                                                                      gCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAAc                                                          <  1:348041/149‑1 (MQ=255)
                                                                                        ctctTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACgg                                                        >  2:514546/1‑149 (MQ=255)
                                                                                        ctctTACCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACgg                                                        >  4:186324/1‑149 (MQ=255)
                                                                                         tctTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTAGTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACgg                                                        >  4:447682/1‑148 (MQ=255)
                                                                                         tctTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACggg                                                       >  3:181527/1‑149 (MQ=255)
                                                                                         tctTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACgg                                                        <  3:447682/148‑1 (MQ=255)
                                                                                           ttATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTc                                                               >  4:581270/1‑139 (MQ=255)
                                                                                           ttATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTc                                                               <  3:581270/139‑1 (MQ=255)
                                                                                              tctcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTaa                                                  <  1:444655/149‑1 (MQ=255)
                                                                                              tctcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTaa                                                  <  2:115250/149‑1 (MQ=255)
                                                                                                tcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGt                                                >  2:51055/1‑149 (MQ=255)
                                                                                                 cATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGtt                                               <  2:153644/149‑1 (MQ=255)
                                                                                                 cATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGtt                                               <  2:85/149‑1 (MQ=255)
                                                                                                  aTTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActct                                                                                                                             <  2:63499/70‑1 (MQ=255)
                                                                                                  aTTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTaa                                                  <  4:493936/145‑1 (MQ=255)
                                                                                                  aTTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTaa                                                  >  3:493936/1‑145 (MQ=255)
                                                                                                  aTTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTg                                              >  1:529809/1‑149 (MQ=255)
                                                                                                  aTTGAAATAGTGTAGTCGGCGTCACAAAAAGATGCGCTCTTACGGAATTTTCCGTAAAGTTCGGTActct                                                                                                                             >  1:63499/1‑70 (MQ=255)
                                                                                                   ttGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGc                                             >  4:499082/1‑149 (MQ=255)
                                                                                                    tGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCt                                            <  2:681424/149‑1 (MQ=255)
                                                                                                    tGAAATAGCGTAGGCGGCTGCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTACGGTACTCTGAGAAAGTAGAGATAAATTCTGCCGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCt                                            <  2:149877/149‑1 (MQ=255)
                                                                                                     gAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTg                                           >  3:105527/1‑149 (MQ=255)
                                                                                                         tAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAg                                       >  3:142582/1‑149 (MQ=255)
                                                                                                         tAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAg                                       >  2:310089/1‑149 (MQ=255)
                                                                                                               aGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActct                                                                                                                             <  2:616024/57‑1 (MQ=255)
                                                                                                               aGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActct                                                                                                                             >  1:616024/1‑57 (MQ=255)
                                                                                                                 tCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGtt                               <  2:531758/149‑1 (MQ=255)
                                                                                                                       tCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCAttt                         >  2:291788/1‑149 (MQ=255)
                                                                                                                         acaAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTAc                       <  3:499082/149‑1 (MQ=255)
                                                                                                                           aaaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTg                     >  1:519353/1‑149 (MQ=255)
                                                                                                                           aaaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTg                     >  4:261732/1‑149 (MQ=255)
                                                                                                                                        tttACGGAAAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTg                                                                                  >  3:643802/2‑75 (MQ=255)
                                                                                                                                        tttACGGAAAATTCCGTAAAGGTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTg                                                                                  <  4:643802/74‑1 (MQ=255)
                                                                                                                                              gAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggtgg  <  1:291788/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AACGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGG  >  NC_000913/824427‑824717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: