Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,244,289 G→A 6.1% A80V (GCG→GTG)  ycgR ← flagellar velocity braking protein, c‑di‑GMP‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,244,2890GA 6.1% 210.4 / 2.9 65A80V (GCG→GTG) ycgRflagellar velocity braking protein, c‑di‑GMP‑regulated
Reads supporting (aligned to +/- strand):  ref base G (36/25);  new base A (2/2);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCAACTGA  >  NC_000913/1244141‑1244433
                                                                                                                                                    |                                                                                                                                                
gCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCg                                                                                                                                                  >  2:426509/1‑149 (MQ=255)
 cAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGc                                                                                                                                                 >  3:498012/1‑149 (MQ=255)
        aaGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGa                                                                                                                                          <  3:533566/149‑1 (MQ=255)
           gCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGGTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGtt                                                                                                                                       >  2:171040/1‑149 (MQ=255)
             ggATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTc                                                                                                                                     >  2:137318/1‑149 (MQ=255)
             ggATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTc                                                                                                                                     >  3:337498/1‑149 (MQ=255)
                tGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTcggc                                                                                                                                  <  1:490712/149‑1 (MQ=255)
                    gtgtGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggta                                                                                                                              >  4:653607/1‑149 (MQ=255)
                       gggCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatg                                                                                                                           <  1:39309/149‑1 (MQ=255)
                       gggCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatg                                                                                                                           <  3:375456/149‑1 (MQ=255)
                         gCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatggt                                                                                                                         >  2:492787/1‑149 (MQ=255)
                           ggAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatggtaa                                                                                                                       >  1:12063/1‑149 (MQ=255)
                                   ggAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAAtgtgctgt                                                                                                               >  2:155874/1‑149 (MQ=255)
                                   ggAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCACACCCTGAGTTTCGGCGGTAATGGTAAtgtgctgt                                                                                                               >  3:314482/1‑149 (MQ=255)
                                        tatCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCtt                                                                                                          <  1:492787/149‑1 (MQ=255)
                                         atCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCttt                                                                                                         <  2:159421/149‑1 (MQ=255)
                                         atCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGGAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCttt                                                                                                         <  2:299469/149‑1 (MQ=255)
                                          tCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTcggc                                                                                                                                  >  3:654410/1‑123 (MQ=255)
                                          tCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTcggc                                                                                                                                  <  4:654410/123‑1 (MQ=255)
                                          tCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTa                                                                                                        >  3:158620/1‑149 (MQ=255)
                                           cGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAg                                                                                                       >  2:577880/1‑149 (MQ=255)
                                                 cgtTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGc                                                                                                 <  1:137318/149‑1 (MQ=255)
                                                 cgtTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCACACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGc                                                                                                 <  4:314482/149‑1 (MQ=255)
                                                  gtTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCg                                                                                                >  4:612821/1‑149 (MQ=255)
                                                      tACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGAtgt                                                                                            >  2:146848/1‑149 (MQ=255)
                                                       aCAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGAtgtt                                                                                           >  4:610454/1‑149 (MQ=255)
                                                       aCAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGAtgtt                                                                                           <  4:374826/149‑1 (MQ=255)
                                                          aaCCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTc                                                                                        <  3:612821/149‑1 (MQ=255)
                                                            ccATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatggtaat                                                                                                                      <  2:628173/117‑1 (MQ=255)
                                                            ccATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatggtaat                                                                                                                      >  1:628173/1‑117 (MQ=255)
                                                             cATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTc                                                                                     <  3:610454/149‑1 (MQ=255)
                                                             cATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTc                                                                                     >  1:534350/1‑149 (MQ=255)
                                                             cATAAGATGGGAGGCGGTAGGGTAATAAATGCCGGAAGCTGCAAGTATTCAGTCTGCTGTAGTTGTTCAAAAGTAAACTCGAGTTTCGCACCCTGAGTTTCGGCGGGAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTc                                                                                     >  4:577355/1‑149 (MQ=255)
                                                              aTAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCg                                                                                    >  1:168412/1‑149 (MQ=255)
                                                               tAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaa                                                                                                                             >  4:287610/1‑107 (MQ=255)
                                                               tAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaa                                                                                                                             <  3:287610/107‑1 (MQ=255)
                                                                    tGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGa                                                                              <  4:498012/149‑1 (MQ=255)
                                                                       gAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTg                                                                           >  1:482171/1‑149 (MQ=255)
                                                                                   taataaATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGc                                                               >  3:507441/1‑149 (MQ=255)
                                                                                                 aGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCgg                                                 <  1:697771/149‑1 (MQ=255)
                                                                                                 aGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCgg                                                 <  2:65157/149‑1 (MQ=255)
                                                                                                    tGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGt                                              >  4:144415/1‑149 (MQ=255)
                                                                                                     gCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCACACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGtt                                             >  3:500067/1‑149 (MQ=255)
                                                                                                           aTTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGcc                                       <  1:155874/149‑1 (MQ=255)
                                                                                                            ttCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCa                                      <  2:46744/149‑1 (MQ=255)
                                                                                                               aCTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGATTCGGCGGTACTGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTa                                   >  4:100441/1‑149 (MQ=255)
                                                                                                                 tctGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAAt                                 >  4:505283/1‑149 (MQ=255)
                                                                                                                   tgctgTGGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAAttt                               >  2:496542/1‑149 (MQ=255)
                                                                                                                      tgTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCt                            >  3:359156/1‑149 (MQ=255)
                                                                                                                          gttgttCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCtt                                                                                      <  3:494111/87‑1 (MQ=255)
                                                                                                                          gttgttCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCtt                                                                                      >  4:494111/1‑87 (MQ=255)
                                                                                                                          gttgttCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATc                        >  2:24119/1‑149 (MQ=255)
                                                                                                                          gttgttCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATc                        >  3:372175/1‑149 (MQ=255)
                                                                                                                          gttgttCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATc                        >  3:266748/1‑149 (MQ=255)
                                                                                                                          gttgttCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATc                        <  2:12063/149‑1 (MQ=255)
                                                                                                                                    aGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGc              >  4:144179/1‑149 (MQ=255)
                                                                                                                                     gTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGcc             >  2:160594/1‑149 (MQ=255)
                                                                                                                                         aCTCGACTTTCGCACCCTGGGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAACTCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTc         <  4:394127/149‑1 (MQ=255)
                                                                                                                                           tCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCa       >  3:177655/1‑149 (MQ=255)
                                                                                                                                           tCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCa       >  2:239248/1‑149 (MQ=255)
                                                                                                                                           tCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCa       >  1:281769/1‑149 (MQ=255)
                                                                                                                                            cGACTTTCACACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCAAGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCaa      <  4:500067/149‑1 (MQ=255)
                                                                                                                                             gACTTTCGCAGCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGGACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCAAc     <  3:241287/149‑1 (MQ=255)
                                                                                                                                             gACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCAAc     <  4:372175/149‑1 (MQ=255)
                                                                                                                                             gACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCAAc     >  1:498555/1‑149 (MQ=255)
                                                                                                                                                tttCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCAACTga  <  1:426509/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATGTGCTGTGCCTTTAGCACGGCGATGTTGTCTTCGGCTTGACTGCCGAAATCCAGCACCAGTTTATCCGGGGTTATTGCCAGTAATTTGCTGATCAGCTGCCCGCCATTCCAACTGA  >  NC_000913/1244141‑1244433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑