Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,689,821 T→G 9.3% intergenic (+70/‑31) manA → / → ydgA mannose‑6‑phosphate isomerase/DUF945 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,689,8210TG 9.3% 272.7 / 21.2 86intergenic (+70/‑31)manA/ydgAmannose‑6‑phosphate isomerase/DUF945 family protein
Reads supporting (aligned to +/- strand):  ref base T (38/40);  new base G (4/4);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

GTTTATTGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACATGGTCGCGCA  >  NC_000913/1689676‑1689967
                                                                                                                                                 |                                                                                                                                                  
gTTTATTGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCg                                                                                                                                                 >  4:249451/1‑149 (MQ=255)
 tttATTGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGc                                                                                                                                                >  1:87606/1‑149 (MQ=255)
     ttGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGa                                                                                                                                            <  2:102214/149‑1 (MQ=255)
     ttGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGa                                                                                                                                            <  3:249451/149‑1 (MQ=255)
      tGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGAt                                                                                                                                           >  3:637083/1‑149 (MQ=255)
      tGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCTGaa                                                                                                                                           <  2:624533/149‑2 (MQ=255)
      tGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGACTGGCCGCCTGaa                                                                                                                                           <  1:404175/149‑2 (MQ=255)
         cgccAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTCCAGGCGGACTGGCCGCCTGaaggt                                                                                                                                        <  3:113968/149‑5 (MQ=255)
          gccAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGaa                                                                                                                                           <  1:594551/145‑2 (MQ=255)
          accAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGaa                                                                                                                                           >  2:594551/2‑144 (MQ=255)
            cAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCAtt                                                                                                                                     <  3:567015/149‑1 (MQ=255)
               cGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTa                                                                                                                                  <  3:577963/149‑1 (MQ=255)
                gAATCACCGGTGACTGTCAAAGGCCGCGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTAt                                                                                                                                 >  4:140890/1‑149 (MQ=255)
                gAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGt                                                                                                                                                    <  2:177060/130‑1 (MQ=255)
                gAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGt                                                                                                                                                    >  1:177060/1‑130 (MQ=255)
                         gTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCa                                                                                                                        <  2:218784/149‑1 (MQ=255)
                          tGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCAt                                                                                                                       >  1:255605/1‑149 (MQ=255)
                             cTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTAt                                                                                                                    <  1:420537/149‑1 (MQ=255)
                               gTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGa                                                                                                                  >  4:433155/1‑149 (MQ=255)
                                tCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGaa                                                                                                                 <  4:637083/149‑1 (MQ=255)
                                tCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGaa                                                                                                                 <  1:108686/149‑1 (MQ=255)
                                tCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGaa                                                                                                                 >  4:545362/1‑149 (MQ=255)
                                 cAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAAt                                                                                                                <  1:634735/149‑1 (MQ=255)
                                  aaaGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTAt                                                                                                                                 <  2:4811/131‑1 (MQ=255)
                                  aaaGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTAt                                                                                                                                 >  1:4811/1‑131 (MQ=255)
                                       ccACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCg                                                                                                          >  2:20835/1‑149 (MQ=255)
                                                          gtTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCa                                                                                       >  3:143711/1‑149 (MQ=255)
                                                            ttACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAtt                                                                                     <  1:477650/149‑1 (MQ=255)
                                                               caacaaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttgtt                                                                                  <  4:207424/149‑1 (MQ=255)
                                                                aacaaGCTGTAAGAGCTTACTGAAAAAACTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttgttg                                                                                 >  4:478266/1‑149 (MQ=255)
                                                                 acaaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTgc                                                                                <  2:107996/149‑1 (MQ=255)
                                                                   aaGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTgcgc                                                                              >  3:651731/1‑149 (MQ=255)
                                                                     gCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTa                                                                            >  4:519090/1‑149 (MQ=255)
                                                                            gagCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAg                                                                     >  2:312285/1‑149 (MQ=255)
                                                                                    tGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACa                                                             >  4:340611/1‑149 (MQ=255)
                                                                                      aaaaaaTTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAgg                                                           >  4:77758/1‑149 (MQ=255)
                                                                                        aaaaTTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAggcg                                                         >  3:413205/1‑149 (MQ=255)
                                                                                         aaaTTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAggcgg                                                        <  3:497346/149‑1 (MQ=255)
                                                                                           aTTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGgcggcg                                                      >  2:256671/1‑149 (MQ=255)
                                                                                            ttAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGgcgc                                                     >  1:271793/1‑149 (MQ=255)
                                                                                            ttAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGgcgc                                                     <  2:273070/149‑1 (MQ=255)
                                                                                            ttAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGgcgc                                                     >  4:461393/1‑149 (MQ=255)
                                                                                               aCATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATg                                                  <  2:255605/149‑1 (MQ=255)
                                                                                               aCATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATg                                                  <  3:17125/149‑1 (MQ=255)
                                                                                                cATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATgg                                                 <  2:123163/149‑1 (MQ=255)
                                                                                                cATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATgg                                                 <  4:651731/149‑1 (MQ=255)
                                                                                                 aTCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGt                                                >  3:516493/1‑149 (MQ=255)
                                                                                                      ttGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACa                                           <  1:312285/149‑1 (MQ=255)
                                                                                                      ttGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACa                                           <  3:340611/149‑1 (MQ=255)
                                                                                                         cTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGc                                        >  1:151110/1‑149 (MQ=255)
                                                                                                               ttattaAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCaagaag                                  >  3:100384/1‑149 (MQ=255)
                                                                                                                tattaAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAagaaga                                 <  3:357578/149‑1 (MQ=255)
                                                                                                                 attaAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGAt                                <  3:519090/149‑1 (MQ=255)
                                                                                                                  ttaAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGAtt                               <  2:271793/149‑1 (MQ=255)
                                                                                                                    aaaGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGa                             >  4:285744/1‑149 (MQ=255)
                                                                                                                     aaGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGaa                            >  3:481263/1‑149 (MQ=255)
                                                                                                                     aaGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGaa                            >  1:182519/1‑149 (MQ=255)
                                                                                                                      aGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGaaa                           <  2:151110/149‑1 (MQ=255)
                                                                                                                        gCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGGAGATTGAAAcc                         >  3:444089/1‑149 (MQ=255)
                                                                                                                          ttATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCa                       >  4:377937/1‑149 (MQ=255)
                                                                                                                            ataACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCAtc                     <  3:818/149‑1 (MQ=255)
                                                                                                                            ataACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCAtc                     <  3:306574/149‑1 (MQ=255)
                                                                                                                               acacCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTGGGCGTAGTCTGGACAGGCGGCGCAGGGTATACAGGCAAGAAGATTGAAACCCATCTCg                  >  4:451688/1‑149 (MQ=255)
                                                                                                                               acacCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCg                  >  2:115491/1‑149 (MQ=255)
                                                                                                                                cacCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGa                 >  3:80676/1‑149 (MQ=255)
                                                                                                                                 acCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttg                                                                                    >  3:282918/1‑81 (MQ=255)
                                                                                                                                 acCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCAttg                                                                                    <  4:282918/81‑1 (MQ=255)
                                                                                                                                 acCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGaa                <  4:638856/149‑1 (MQ=255)
                                                                                                                                 acCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGaa                <  3:475943/149‑1 (MQ=255)
                                                                                                                                 acCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGaa                <  3:461393/149‑1 (MQ=255)
                                                                                                                                 acCTTCAGGCGGCCAGTCCGCCAGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGaa                <  3:418589/149‑1 (MQ=255)
                                                                                                                                  ccTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAg               <  3:527999/149‑1 (MQ=255)
                                                                                                                                   cTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCa                                                                                       <  1:456470/76‑1 (MQ=255)
                                                                                                                                   cTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCa                                                                                       >  2:456470/1‑76 (MQ=255)
                                                                                                                                   cTTCAGGCGGCCAGTCCGCCTGATTTCATTTCATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGa              >  2:38303/1‑149 (MQ=255)
                                                                                                                                   aatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGAtt                               <  1:275942/130‑1 (MQ=255)
                                                                                                                                   aatCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGAtt                               >  2:275942/3‑132 (MQ=255)
                                                                                                                                    ttCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGAc             >  1:585146/1‑149 (MQ=255)
                                                                                                                                    atCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGAc             >  2:200129/2‑149 (MQ=255)
                                                                                                                                    atCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGAc             >  2:310489/2‑149 (MQ=255)
                                                                                                                                     tCAGGCGGACTGGCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACa            >  1:453772/1‑149 (MQ=255)
                                                                                                                                      cAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTg                              <  4:43873/130‑1 (MQ=255)
                                                                                                                                      cAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTg                              >  3:43873/1‑130 (MQ=255)
                                                                                                                                      cAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACAt           <  1:97387/149‑1 (MQ=255)
                                                                                                                                       aGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACATg          <  2:585146/149‑1 (MQ=255)
                                                                                                                                            gcCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTTGTAGCGGTAGTCGTCATTGTTTCGCTAGGCGTAGTCTGGACAGGCGTCGCATGGTATACAGGCAAGTAGATTGAAACATATCTCGAAGACATGGTcga     >  3:677880/1‑148 (MQ=255)
                                                                                                                                            gcCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACATGGTcgc     <  2:625061/149‑1 (MQ=255)
                                                                                                                                               aGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACATGGTCGCGCa  <  2:347817/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GTTTATTGCCGCCAACGAATCACCGGTGACTGTCAAAGGCCACGGCCGTTTAGCGCGTGTTTACAACAAGCTGTAAGAGCTTACTGAAAAAATTAACATCTCTTGCTAAGCTTATTAAAGGCTTATAACACCTTCAGGCGGCCAGTCCGCCTGATTTCATTTTATGGATAATCATTATGAATAAATCGCTGGTAGCGGTAGGCGTCATTGTTGCGCTAGGCGTAGTCTGGACAGGCGGCGCATGGTATACAGGCAAGAAGATTGAAACCCATCTCGAAGACATGGTCGCGCA  >  NC_000913/1689676‑1689967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: