| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,520,500 | Δ1 bp | 20.9% | intergenic (‑176/+171) | yjhU ← / ← yjhF | putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,520,500 | 0 | A | . | 20.9% | 128.3 / 30.2 | 43 | intergenic (‑176/+171) | yjhU/yjhF | putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter |
| Reads supporting (aligned to +/- strand): ref base A (19/15); new base . (5/4); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01 | |||||||||||
AATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAG > NC_000913/4520352‑4520646 | aaTGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGgaga < 1:589430/149‑1 (MQ=255) ttAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACa > 4:425047/1‑141 (MQ=255) ttAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACa < 3:425047/141‑1 (MQ=255) ttAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCgg > 1:255653/1‑149 (MQ=255) tAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGc < 2:61243/149‑1 (MQ=255) aaCCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACTAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt < 4:462401/149‑1 (MQ=255) ccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa < 1:234258/149‑1 (MQ=255) cGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACt > 3:155860/1‑149 (MQ=255) ttGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCttt > 1:290833/1‑131 (MQ=255) ttGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCttt < 2:290833/131‑1 (MQ=255) gATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAccc < 2:98036/146‑1 (MQ=255) gATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAccc > 1:98036/1‑146 (MQ=255) ccAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGaa < 2:6406/134‑1 (MQ=255) ccAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGaa > 1:6406/1‑134 (MQ=255) cAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCAt > 3:802543/1‑149 (MQ=255) tGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg < 4:155860/149‑1 (MQ=255) aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGt < 2:255653/149‑1 (MQ=255) ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt > 2:362300/1‑84 (MQ=25) ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt < 1:362300/84‑1 (MQ=25) ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGgaga > 3:172235/1‑71 (MQ=255) ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGgaga < 4:172235/71‑1 (MQ=255) ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg > 1:64784/1‑125 (MQ=255) ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg < 2:64784/125‑1 (MQ=255) tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg > 3:323580/1‑149 (MQ=255) aCCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCa < 4:802543/149‑1 (MQ=255) gCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTa > 2:757945/1‑75 (MQ=255) gCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTa < 1:757945/75‑1 (MQ=255) tttGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt > 2:416591/1‑131 (MQ=255) tttGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt < 1:416591/131‑1 (MQ=255) aGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGc > 3:153074/1‑149 (MQ=255) cGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcac < 2:135819/149‑1 (MQ=255) cccGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg > 4:524939/1‑86 (MQ=255) cccGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg < 3:524939/86‑1 (MQ=255) ccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGc > 2:98858/1‑131 (MQ=255) ccccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGc < 1:98858/131‑1 (MQ=255) ccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTaaa > 1:114878/1‑143 (MQ=255) ccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTaaa < 2:114878/143‑1 (MQ=255) ccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgg > 4:769586/1‑149 (MQ=255) tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTAGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACa > 1:237630/1‑149 (MQ=255) aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAA‑TATGGGGGGCaagga > 2:141024/1‑145 (MQ=255) aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGa > 3:562306/1‑149 (MQ=255) aGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATAGACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaa > 3:696929/1‑149 (MQ=255) aGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaa > 3:35847/1‑149 (MQ=255) aGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaa > 1:138476/1‑149 (MQ=255) aGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTCCAATATCGCCCCTTTTTGATCGCCAGCGATTTGTCACCGTGCAGAGGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaa > 1:221578/1‑149 (MQ=255) cGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaacaa < 3:769586/149‑1 (MQ=255) agaCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAg < 4:323580/149‑1 (MQ=255) | AATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTT‑CCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAG > NC_000913/4520352‑4520646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |