Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,330,419:1 | +T | 5.6% | intergenic (+23/+163) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,330,419 | 1 | . | T | 5.6% | 309.5 / 13.0 | 71 | intergenic (+23/+163) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
Reads supporting (aligned to +/- strand): ref base . (28/39); new base T (2/2); total (30/41) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-02 |
AACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGC > NC_000913/3330272‑3330566 | aaCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGGAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGCACAATTAGTCAAAAAGAAACCCCCGCACATg‑g > 3:499341/1‑149 (MQ=255) aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGAGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑Gc > 2:691852/1‑149 (MQ=255) aTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCggt < 4:515013/149‑2 (MQ=255) aTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCggg < 4:652585/149‑1 (MQ=255) aTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCggg > 3:313631/1‑149 (MQ=255) gTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTc < 4:297246/149‑1 (MQ=255) cAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat < 2:427777/149‑1 (MQ=255) aaTATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattatt < 3:562410/149‑1 (MQ=255) aaTATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattatt < 2:696973/149‑1 (MQ=255) aaTATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattatt < 4:547008/149‑1 (MQ=255) tatCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGa < 3:241452/149‑1 (MQ=255) tCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACa < 4:213973/149‑1 (MQ=255) tttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAGCCCCGGC‑CATGTGCCGGGGTTTcttttt > 2:216778/1‑135 (MQ=255) tttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAGCCCCGGC‑CATGTGCCGGGGTTTcttttt < 1:216778/140‑6 (MQ=255) tttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGTCaaa < 1:691852/149‑1 (MQ=255) gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTgcgc > 2:207968/1‑149 (MQ=255) gCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGt > 4:798751/1‑149 (MQ=255) aGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCa > 1:704446/1‑123 (MQ=255) aGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCa < 2:704446/123‑1 (MQ=255) gTAGAACCTCCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGa < 4:9827/124‑1 (MQ=255) gTAGAACCTCCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGa > 3:9827/1‑124 (MQ=255) cTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat > 4:276138/1‑121 (MQ=255) cTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat < 3:276138/121‑1 (MQ=255) gATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg < 3:772974/149‑1 (MQ=255) aTCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGt < 4:41711/124‑1 (MQ=255) aTCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGt > 3:41711/1‑124 (MQ=255) gCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa > 1:710788/1‑149 (MQ=255) tAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACg > 2:715666/1‑149 (MQ=255) aaTCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGc < 3:798751/149‑1 (MQ=255) gCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTg < 2:727900/120‑1 (MQ=255) gCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTg > 1:727900/1‑120 (MQ=255) tATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg < 1:159782/149‑1 (MQ=255) tCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc < 1:198643/149‑1 (MQ=255) gTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCtt < 4:612080/138‑1 (MQ=255) gTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCtt > 3:612080/1‑138 (MQ=255) gTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCa < 4:253462/83‑1 (MQ=255) gTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCa > 3:253462/1‑83 (MQ=255) gTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa > 3:85569/1‑149 (MQ=255) gTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGt < 2:789219/149‑1 (MQ=255) gCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa > 1:776482/1‑149 (MQ=255) ttGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc < 3:437492/124‑1 (MQ=255) ttGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc > 4:437492/1‑124 (MQ=255) ttGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata < 2:78700/149‑1 (MQ=255) tGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat < 3:79501/149‑1 (MQ=255) tataAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg > 3:291058/1‑149 (MQ=255) aaaGCTATTTATCAGAACAATTAGTAAAAAAGAAACCCCAGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa < 4:499341/149‑1 (MQ=255) aaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAt < 4:85569/149‑1 (MQ=255) aGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa < 4:285534/147‑1 (MQ=255) aGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa > 3:285534/1‑147 (MQ=255) gATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAc > 3:704332/1‑137 (MQ=255) gATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAc < 4:704332/137‑1 (MQ=255) aTCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa < 4:313631/149‑1 (MQ=255) cAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTAt < 1:233980/75‑1 (MQ=255) cAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTAt > 2:233980/1‑75 (MQ=255) aGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGc > 3:416117/1‑149 (MQ=255) gAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGcc > 3:297474/1‑149 (MQ=255) gAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGcc < 3:685775/149‑1 (MQ=255) aaCAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCt < 1:715666/149‑1 (MQ=255) aGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGc < 4:291058/149‑1 (MQ=255) aGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGc > 3:323160/1‑149 (MQ=255) aGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGc < 2:710788/149‑1 (MQ=255) tCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGt > 4:304673/1‑149 (MQ=255) cAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTa < 1:207968/149‑1 (MQ=255) aaaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATa > 1:135633/1‑142 (MQ=255) aaaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATa < 2:135633/142‑1 (MQ=255) aaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATTCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAgc > 4:118454/1‑149 (MQ=255) gAAGCCCCGGC‑CATGTGCCGGGGTTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGc < 2:86444/131‑1 (MQ=18) gAAGCCCCGGC‑CATGTGCCGGGGTTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGc > 1:86444/1‑131 (MQ=18) aaaCCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATc < 2:776482/149‑1 (MQ=255) aTG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTgg > 3:679626/1‑149 (MQ=255) aTG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTgg > 2:329606/1‑149 (MQ=255) tG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGc < 4:416117/149‑1 (MQ=255) | AACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGC > NC_000913/3330272‑3330566 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |