Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 574,486:1 | +C | 9.5% | intergenic (+147/+43) | quuD → / ← nmpC | DLP12 prophage; putative antitermination protein/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 574,486 | 1 | . | C | 9.5% | 175.2 / 14.4 | 42 | intergenic (+147/+43) | quuD/nmpC | DLP12 prophage; putative antitermination protein/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage |
Reads supporting (aligned to +/- strand): ref base . (24/14); new base C (2/2); total (26/16) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.28e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 |
TTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCA > NC_000913/574348‑574632 | ttGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAAc < 3:497950/149‑1 (MQ=255) tACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTc < 2:31498/149‑1 (MQ=255) tAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGa < 1:543110/135‑1 (MQ=255) tAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGa > 2:543110/1‑135 (MQ=255) aaTTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTg < 3:660733/127‑1 (MQ=255) agaCGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGAtt > 4:149813/1‑149 (MQ=255) aCGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAg > 1:441041/1‑149 (MQ=255) cccccAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAAc > 1:238228/1‑149 (MQ=255) aaTAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTa > 3:802375/1‑146 (MQ=255) aaTAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTa < 4:802375/146‑1 (MQ=255) tatataACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGc > 1:637739/1‑149 (MQ=255) ataACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAAc < 3:149813/149‑1 (MQ=255) ataACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAAc > 4:597246/1‑149 (MQ=255) aaCAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTGATATGTTGCGGGAGGCGTTGCCTCCC‑CAACatataa < 1:545778/103‑3 (MQ=25) aaCAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTGATATGTTGCGGGAGGCGTTGCCTCCC‑CAACatataa > 2:545778/1‑101 (MQ=25) aGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGt > 1:341386/1‑149 (MQ=255) atataGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGc < 4:4235/70‑1 (MQ=255) atataGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGc > 3:4235/1‑70 (MQ=255) ttAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTacac > 3:469969/1‑149 (MQ=255) ttAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTacac < 2:637739/149‑1 (MQ=255) cAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGc < 2:341386/149‑1 (MQ=255) ttGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAg < 3:597246/149‑1 (MQ=255) gCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGAt < 2:670075/116‑1 (MQ=255) gCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGAt > 1:670075/1‑116 (MQ=255) ttCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAgg > 4:706250/1‑149 (MQ=255) ttCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAgg > 2:672546/1‑149 (MQ=255) ggAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCtttt < 3:766362/63‑1 (MQ=255) ggAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCtttt > 4:766362/1‑63 (MQ=255) cTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCgggg > 1:163102/1‑149 (MQ=255) gAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCggg > 3:276634/1‑141 (MQ=255) gAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCggg < 4:276634/141‑1 (MQ=255) cACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCgg > 2:579961/1‑136 (MQ=255) cACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCgg < 1:579961/136‑1 (MQ=255) cACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTtctc > 4:682780/1‑149 (MQ=255) aCTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCg > 3:511879/1‑149 (MQ=255) aCTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCg > 2:260721/1‑149 (MQ=255) agatatGTTGCGGGAGGCGTTGCCTCCC‑CAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAAtt < 3:467245/138‑1 (MQ=38) agatatGTTGCGGGAGGCGTTGCCTCCC‑CAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAAtt > 4:467245/3‑140 (MQ=38) tGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGCTGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACt > 1:227604/1‑149 (MQ=255) gTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAgg < 2:457898/109‑1 (MQ=255) gTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAgg > 1:457898/1‑109 (MQ=255) gTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTc > 4:519728/1‑149 (MQ=255) ttG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa > 3:661431/1‑149 (MQ=255) g‑gggAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAAc > 4:220028/1‑58 (MQ=255) g‑gggAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAAc < 3:220028/58‑1 (MQ=255) | TTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATAAAGGGTAATATATAACAGAAGGTTTATATAGTTAGAAGCAAGGTTGTGCTTCTAAAGGAAGTGGCTTGAGGGAGCCACTTATATGTTG‑GGGAGGCAACGCCTCCCGCAACATATCTTTTTCGTAATCAGATTAGAACTGGTAAACCAGACCTACAGCAACGATGTCATCAGTGCTTACACCGAGTGCTTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCA > NC_000913/574348‑574632 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |