Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,321,432 | A→T | 7.5% | A505A (GCT→GCA) | trpE ← | component I of anthranilate synthase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,321,432 | 0 | A | T | 7.5% | 376.1 / 17.0 | 107 | A505A (GCT→GCA) | trpE | component I of anthranilate synthase |
Reads supporting (aligned to +/- strand): ref base A (51/48); new base T (3/5); total (54/53) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01 |
Only 100 of 107 total aligned reads displayed.
GGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCC > NC_000913/1321285‑1321569 | ggTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAg > 3:369447/1‑149 (MQ=255) ggTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAg > 1:599743/1‑149 (MQ=255) ttGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAgcg > 4:143806/1‑149 (MQ=255) tGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGACAATAGCGCGCAGTACAgcgc < 3:612701/149‑1 (MQ=255) gCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGcgcg < 4:393910/149‑1 (MQ=255) cGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCTGTACTGcgcgc < 3:370469/149‑2 (MQ=255) tAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCTGTACTGcgcgctat < 3:585356/149‑5 (MQ=255) tAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCt < 2:599743/149‑1 (MQ=255) aaTCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCttt < 3:460098/149‑1 (MQ=255) aTCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTg < 2:515079/149‑1 (MQ=255) caccacGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGtt < 3:531254/149‑1 (MQ=255) cacGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACg > 4:514592/1‑149 (MQ=255) ttATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGtt > 3:745064/1‑149 (MQ=255) tATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGttt > 4:570834/1‑149 (MQ=255) cccATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTtcgtc > 1:225034/1‑149 (MQ=255) cccATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTtcgtc > 2:6625/1‑149 (MQ=255) ccATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTtcgtcg > 2:559969/1‑149 (MQ=255) ccATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTtcgtcg > 1:506488/1‑149 (MQ=255) aTTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGcgcg < 3:339746/125‑1 (MQ=255) aTTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGcgcg > 4:339746/1‑125 (MQ=255) gctgcGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACgg > 1:343157/1‑135 (MQ=255) gctgcGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACgg < 2:343157/135‑1 (MQ=255) gctgcGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTc < 3:33349/149‑1 (MQ=255) tgcGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCg > 3:665670/1‑149 (MQ=255) gcgcAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCGTTGTTACGGGTTTCGTCGGCTTCCGa < 3:496538/149‑1 (MQ=255) aaCTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGc < 3:514592/149‑1 (MQ=255) aaCTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGc > 1:809965/1‑149 (MQ=255) aaCTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGc < 1:559969/149‑1 (MQ=255) aaCTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGc < 2:225034/149‑1 (MQ=255) aCTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCg < 1:330657/149‑1 (MQ=255) tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGa > 4:335942/1‑149 (MQ=255) tGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGa > 2:281928/1‑149 (MQ=255) gCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGaa > 3:176488/1‑149 (MQ=255) cAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATc > 4:491252/1‑149 (MQ=255) ggTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCaa > 1:587876/1‑149 (MQ=255) ggTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCaa < 1:538609/149‑1 (MQ=255) gTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa > 3:405885/1‑144 (MQ=255) gTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa < 4:405885/144‑1 (MQ=255) tAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCaa > 3:362624/1‑140 (MQ=255) tAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCaa < 4:362624/140‑1 (MQ=255) tAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTacac < 3:335942/149‑1 (MQ=255) tAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTacac > 4:281823/1‑149 (MQ=255) aaaaGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCg > 1:190243/1‑127 (MQ=255) aaaaGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCg < 2:190243/127‑1 (MQ=255) aaaaGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACAcc > 3:746149/1‑149 (MQ=255) gagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa > 2:206042/1‑138 (MQ=255) gagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa < 1:206042/138‑1 (MQ=255) gagTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCa < 2:506488/149‑1 (MQ=255) tCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCAccc > 4:680943/1‑149 (MQ=255) tCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCAccc > 1:134766/1‑149 (MQ=255) atatTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa < 3:555059/132‑1 (MQ=255) atatTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa > 4:555059/1‑132 (MQ=255) tatCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCa > 3:615477/1‑149 (MQ=255) tCGAGCATCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACg > 2:440458/1‑149 (MQ=255) tCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa < 2:375360/126‑1 (MQ=255) tCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa > 1:375360/1‑126 (MQ=255) cagcagAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGc < 4:665670/149‑1 (MQ=255) agcagAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCg > 2:344587/1‑149 (MQ=255) gcagAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGa < 3:491252/149‑1 (MQ=255) gAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAg > 1:207174/1‑114 (MQ=255) gAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAg < 4:724107/114‑1 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGttt < 4:531432/149‑1 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGttt > 4:111025/1‑149 (MQ=255) tCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGttt < 2:809965/149‑1 (MQ=255) cAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATTAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGtttt > 3:217876/1‑149 (MQ=255) cAGCCATCAGAAAGGCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGtttt < 4:745064/149‑1 (MQ=255) aGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTc > 1:566401/1‑149 (MQ=255) aGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTc < 2:134766/149‑1 (MQ=255) aGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTc > 1:771452/1‑149 (MQ=255) ccATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTcca < 1:281928/149‑1 (MQ=255) tCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTccacca > 3:27100/1‑149 (MQ=255) tCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTccacca < 4:176488/149‑1 (MQ=255) cAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGttt > 4:426987/1‑141 (MQ=255) cAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGttt < 3:426987/141‑1 (MQ=255) aGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCg < 4:256976/149‑1 (MQ=255) gTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGa > 2:686626/1‑149 (MQ=255) tctcCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAg < 1:445085/149‑1 (MQ=255) tctcCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAg > 1:269022/1‑149 (MQ=255) tcCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTcc > 4:335402/1‑135 (MQ=255) tcCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTcc < 3:335402/135‑1 (MQ=255) cTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa < 3:314713/92‑1 (MQ=255) cTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa > 4:314713/1‑92 (MQ=255) tgtgCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTcc > 4:246803/1‑69 (MQ=255) tgtgCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTcc < 3:246803/69‑1 (MQ=255) tgtgCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGAt > 3:713437/1‑149 (MQ=255) atgatgCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACaa < 3:281823/149‑1 (MQ=255) gTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGgtggc < 1:686626/149‑3 (MQ=255) gTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTc < 3:143806/149‑1 (MQ=255) tGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCg > 1:530650/1‑149 (MQ=255) ggCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCga < 1:344587/149‑1 (MQ=255) aaTAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGAt < 4:412941/149‑1 (MQ=255) aaTAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGAt > 1:743883/1‑149 (MQ=255) aTAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATc > 1:211282/1‑149 (MQ=255) aTAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATc < 3:570834/149‑1 (MQ=255) gcccgcgcTGTACTGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACCGAATCAAGGa < 3:49460/66‑1 (MQ=38) gcccgcgcTGTACTGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGttt > 2:517358/4‑107 (MQ=255) gcccgcgcTGTACTGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGttt < 1:517358/104‑1 (MQ=255) gcccgcgcTGTACTGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGAt > 4:116517/4‑147 (MQ=255) gcccgcgcTGTACTGCGCGGGCTATGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGa > 4:49460/4‑69 (MQ=38) aGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGc < 1:6625/149‑1 (MQ=255) | GGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGTGGCAATAGCGCGCAGTACAGCGCGGGCTTTGTTACGGGTTTCGTCGGCTTCCGACTGCGGAACAGAATCAAGGACTACACCAGCACCCGCTTGCACGGTGGCGATACCGTTTTCCACCAGCGCCGAGCGGATCACAATGCAGGTGTCGAGATCGCC > NC_000913/1321285‑1321569 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |