| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,520,584 | A→G | 5.2% | D108G (GAC→GGC) ‡ | mcbR → | colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,520,584 | 0 | A | G | 5.2% | 250.5 / 3.7 | 77 | D108G (GAC→GGC) ‡ | mcbR | colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled |
| Reads supporting (aligned to +/- strand): ref base A (36/37); new base G (2/2); total (38/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-02 | |||||||||||
GTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAG > NC_000913/1520436‑1520729 | gTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGa < 3:239805/149‑1 (MQ=255) tCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCt < 1:503844/149‑1 (MQ=255) tGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCg > 4:514027/1‑149 (MQ=255) cgcTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTc < 2:181868/149‑1 (MQ=255) cTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCa < 2:776352/149‑1 (MQ=255) gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCt > 3:443117/1‑149 (MQ=255) gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCt < 1:582335/149‑1 (MQ=255) cACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCtg < 4:22086/149‑2 (MQ=255) aCCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCg > 3:532645/1‑149 (MQ=255) acaAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAAt < 1:230277/149‑1 (MQ=255) caAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGaa < 4:783595/126‑1 (MQ=255) caAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGaa > 3:783595/1‑126 (MQ=255) aaGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTa > 3:787566/1‑149 (MQ=255) ttACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAcagcag > 4:586560/1‑149 (MQ=255) tACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAgg > 4:418163/1‑149 (MQ=255) aGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGgcgc > 4:236834/1‑149 (MQ=255) aGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGgcgc > 1:486265/1‑149 (MQ=255) ggAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGaaa > 4:142813/1‑149 (MQ=255) gAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGaaaa < 4:106019/149‑1 (MQ=255) gAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGaaaa < 4:443117/149‑1 (MQ=255) gAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGaaaa < 2:403835/149‑1 (MQ=255) gAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGaaaa > 2:640668/1‑149 (MQ=255) aGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCCTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAgg < 4:537893/149‑1 (MQ=255) tGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAca < 3:315004/130‑1 (MQ=255) tGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAca > 4:315004/1‑130 (MQ=255) ggggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCaa < 4:647401/139‑1 (MQ=255) ggggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCaa > 3:647401/1‑139 (MQ=255) gggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGa > 2:521429/1‑149 (MQ=255) gggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTCGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGTATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGa > 4:675354/1‑149 (MQ=255) gAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACa > 2:735918/1‑149 (MQ=255) aaaCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGGTCTTGCggg < 3:404006/98‑2 (MQ=255) aaaCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGGTCTTGCggg > 4:404006/1‑97 (MQ=255) aaCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATg < 1:563474/149‑1 (MQ=255) tCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAAc > 2:525940/1‑149 (MQ=255) tCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAAc > 4:649188/1‑149 (MQ=255) aaTTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaa < 4:787566/149‑1 (MQ=255) aTTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa < 3:611987/149‑1 (MQ=255) aGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa < 2:288036/149‑1 (MQ=255) aCGAAATCAATCGGAACCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCAtt < 1:409439/149‑1 (MQ=255) aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAt < 3:456/149‑1 (MQ=255) aatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa > 2:255161/1‑143 (MQ=255) aatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa < 1:255161/143‑1 (MQ=255) atcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAc > 3:700927/1‑149 (MQ=255) ggATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg > 2:356648/1‑149 (MQ=255) ggATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg < 1:735918/149‑1 (MQ=255) gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGc > 3:383589/1‑149 (MQ=255) ccGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAt > 2:183431/1‑149 (MQ=255) tACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCg > 1:369528/1‑149 (MQ=255) cGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCt < 3:512145/149‑1 (MQ=255) aCTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAg > 3:578736/1‑149 (MQ=255) cTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGc < 2:91546/149‑1 (MQ=255) tGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCg > 1:97625/1‑149 (MQ=255) aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAtt < 4:383589/149‑1 (MQ=255) ttAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTa < 2:97625/149‑1 (MQ=255) aaTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACATGCTATTTCGCTTAGCGATTTatc < 4:573858/149‑1 (MQ=255) aaTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc > 3:148665/1‑149 (MQ=255) aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatca < 3:236834/149‑1 (MQ=255) aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatca < 3:514027/149‑1 (MQ=255) gCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCaaa < 1:809805/149‑1 (MQ=255) ctctGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAat > 1:766171/1‑149 (MQ=255) tctGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcatc < 4:158851/140‑1 (MQ=255) tctGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcatc > 3:158851/1‑140 (MQ=255) tctGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAata > 3:197104/1‑149 (MQ=255) ggCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGc < 1:183431/149‑1 (MQ=255) tGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCa > 2:184082/1‑149 (MQ=255) tGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCa > 4:341205/1‑149 (MQ=255) aaCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtg < 4:532645/149‑1 (MQ=255) tCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag > 1:6085/1‑149 (MQ=255) cACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgaga < 4:290526/149‑1 (MQ=255) cACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgaga > 3:249444/1‑149 (MQ=255) aCCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgat > 3:391520/1‑149 (MQ=255) ttccGCAAGGTCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAtt < 4:139193/111‑1 (MQ=255) ttccGCAAGGTCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAtt > 3:139193/3‑113 (MQ=255) cccGCAATACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa < 4:481467/80‑1 (MQ=255) cccGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa > 3:481467/1‑80 (MQ=255) ccGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatgat < 4:148665/149‑1 (MQ=255) cAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGa < 3:418163/149‑1 (MQ=255) aaGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAGGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAg < 3:47755/149‑1 (MQ=255) | GTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAG > NC_000913/1520436‑1520729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |