Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,717,186	G→A	5.1%	R13W (CGG→TGG)	tyrS ←	tyrosyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,717,186	0	G	A	5.1%	265.6 / 3.3	78	R13W (CGG→TGG)	tyrS	tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (38/36);  new base A (2/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

GAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTAAAGCA  >  NC_000913/1717040‑1717334                                                                                                                                                   |                                                                                                                                                     gAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGct                                                                                                                                                    >  1:332124/1‑149 (MQ=255)  aaGCGTTTCAGGCATAACAACGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGctc                                                                                                                                                   <  4:385010/149‑1 (MQ=255)   aGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGctct                                                                                                                                                  <  4:232456/149‑1 (MQ=255)          cAGGCATAACAATGGAACAAGACGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAAtt                                                                                                                                           <  1:699733/149‑1 (MQ=255)           aGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCActc                                                                                                                                                   <  1:322024/140‑1 (MQ=255)           aGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCActc                                                                                                                                                   >  2:70676/1‑140 (MQ=255)           aGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCActc                                                                                                                                                   >  2:322024/1‑140 (MQ=255)           aGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCActc                                                                                                                                                   <  1:70676/140‑1 (MQ=255)              cATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGttt                                                                                                                                       >  2:679331/1‑149 (MQ=255)              cATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGttt                                                                                                                                       <  4:606049/149‑1 (MQ=255)               aTAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTa                                                                                                                                      >  3:177084/1‑149 (MQ=255)                    aaTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCaa                                                                                                                                 <  1:679331/149‑1 (MQ=255)                     aTGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAg                                                                                                                                >  1:18126/1‑149 (MQ=255)                      tGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGt                                                                                                                               <  3:706359/149‑1 (MQ=255)                      tGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGt                                                                                                                               <  2:371530/149‑1 (MQ=255)                         aaCAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTAc                                                                                                                            >  4:480071/1‑149 (MQ=255)                              gATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCtt                                                                                                                       <  3:236438/149‑1 (MQ=255)                               aTGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTg                                                                                                                      <  3:242417/149‑1 (MQ=255)                                   cccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCAt                                                                                                                  >  4:384951/1‑149 (MQ=255)                                    ccAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATc                                                                                                                 >  1:69474/1‑149 (MQ=255)                                     cAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATcc                                                                                                                >  4:543073/1‑148 (MQ=255)                                     cAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCa                                                                                                                >  1:90117/1‑149 (MQ=255)                                     cAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCa                                                                                                                >  3:478448/1‑149 (MQ=255)                                             aGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTcc                                                                                                        <  2:561273/149‑1 (MQ=255)                                              gCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCa                                                                                                       <  3:384951/149‑1 (MQ=255)                                               cTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCAt                                                                                                      <  4:386372/149‑1 (MQ=255)                                                tGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATg                                                                                                     >  3:708087/1‑149 (MQ=255)                                                    aGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCAt                                                                                                      <  3:464772/144‑1 (MQ=255)                                                    aGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTGGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCAt                                                                                                      >  4:464772/1‑144 (MQ=255)                                                        gTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAAc                                                                                             >  1:602137/1‑149 (MQ=255)                                                          aGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGa                                                                                           <  2:18126/149‑1 (MQ=255)                                                           ggATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGAc                                                                                          <  4:84848/149‑1 (MQ=255)                                                            gATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACt                                                                                         >  1:428012/1‑149 (MQ=255)                                                             aTCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTg                                                                                        >  4:783537/1‑149 (MQ=255)                                                             aTCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTg                                                                                        >  3:730185/1‑149 (MQ=255)                                                             aTCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTg                                                                                        >  4:542633/1‑149 (MQ=255)                                                             aTCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTg                                                                                        >  1:38358/1‑149 (MQ=255)                                                                       cAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGcc                                                                              <  3:2608/149‑1 (MQ=255)                                                                         aTAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCgg                                                                            >  1:460741/1‑149 (MQ=255)                                                                                   aTCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACtt                                                                  <  4:730185/149‑1 (MQ=255)                                                                                    tCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGc                                                                                                                     >  2:395354/1‑97 (MQ=255)                                                                                    tCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGc                                                                                                                     <  1:395354/97‑1 (MQ=255)                                                                                     cGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGATCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGCATATAACGACTGCACCTTTGCCGGTACACGACtttt                                                                >  4:472425/1‑149 (MQ=255)                                                                                       ggCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCg                                                              >  2:516110/1‑149 (MQ=255)                                                                                       ggCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCg                                                              >  3:537950/1‑149 (MQ=255)                                                                                        gCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGc                                                             <  3:73555/149‑1 (MQ=255)                                                                                          cTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCa                                                           >  1:244848/1‑149 (MQ=255)                                                                                               gcCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGc                                                      <  2:332124/149‑1 (MQ=255)                                                                                                   gTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGaaa                                                  >  4:223648/1‑149 (MQ=255)                                                                                                     cGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAaga                                                <  2:69474/149‑1 (MQ=255)                                                                                                           gCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATa                                          <  2:602137/149‑1 (MQ=255)                                                                                                             tAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGaaa                                                  <  1:584823/139‑1 (MQ=255)                                                                                                             tAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGaaa                                                  >  2:584823/1‑139 (MQ=255)                                                                                                               aCGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAAt                                      >  3:193082/1‑149 (MQ=255)                                                                                                               aCGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTCAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAAt                                      >  4:251206/1‑149 (MQ=255)                                                                                                                 gCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCGATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATaa                                    >  2:517222/1‑149 (MQ=255)                                                                                                                  cTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATaaa                                   <  2:428012/149‑1 (MQ=255)                                                                                                                     ccTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTTCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACAAAGAATAAAGTg                                >  1:482524/1‑149 (MQ=255)                                                                                                                      cTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGc                               <  2:482524/149‑1 (MQ=255)                                                                                                                       tCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGcc                              >  3:2913/1‑149 (MQ=255)                                                                                                                       tCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGcc                              <  2:244848/149‑1 (MQ=255)                                                                                                                            cgtcACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAAt                         <  3:480071/149‑1 (MQ=255)                                                                                                                             gtcACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATc                        >  4:273154/1‑149 (MQ=255)                                                                                                                              tcACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCa                       <  4:708087/149‑1 (MQ=255)                                                                                                                                 ccTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATTTAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGa                    <  3:542633/149‑1 (MQ=255)                                                                                                                                 ccTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGa                    <  4:177084/149‑1 (MQ=255)                                                                                                                                 ccTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGa                    <  4:2913/149‑1 (MQ=255)                                                                                                                                          ccAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCgg                                                                            <  1:723237/84‑1 (MQ=255)                                                                                                                                          ccAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCgg                                                                            >  2:723237/1‑84 (MQ=255)                                                                                                                                          ccAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCg           >  2:286961/1‑149 (MQ=255)                                                                                                                                            aGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCCCCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGAt         <  3:543073/149‑1 (MQ=255)                                                                                                                                            aGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGAt         <  4:537950/149‑1 (MQ=255)                                                                                                                                                cccGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGAc                                                                    >  3:767553/1‑86 (MQ=255)                                                                                                                                                cccGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGAc                                                                    <  4:767553/86‑1 (MQ=255)                                                                                                                                                cccGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTaaa     >  1:605749/1‑149 (MQ=255)                                                                                                                                                cccGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTaaa     >  3:637827/1‑149 (MQ=255)                                                                                                                                                 ccGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAAt                                      <  2:157510/115‑1 (MQ=255)                                                                                                                                                 ccGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAAt                                      >  1:157510/1‑115 (MQ=255)                                                                                                                                                   gCTCTTGCGATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTAAAGCa  <  1:517222/149‑1 (MQ=255)                                                                                                                                                   gCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTAAAGCa  <  3:273154/149‑1 (MQ=255)                                                                                                                                                   gCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTAAAGCa  >  4:380721/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGCCTTGCGCCAGTCGCTCTGCTAACGCTTCCTCGTCCGTCACCTGGGCTACCAGCCCCCGCTCTTGCAATTGTTTAATCAAGTTACTGCTTGCCATCAAAATCTCCATGTATATAACGACTGCACCTTTGCCGGTACACGACTTTTCGCCAGATGCGAAAGAGACATAGAATAAAGTGCCAGAATCAGGAGTACCAGCGATTAAAGCA  >  NC_000913/1717040‑1717334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑