Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,763,267 A→G 5.4% V248A (GTC→GCC)  sufB ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,763,2670AG 5.4% 243.0 / 3.1 74V248A (GTC→GCC) sufBcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base A (34/36);  new base G (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

CGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATT  >  NC_000913/1763124‑1763415
                                                                                                                                               |                                                                                                                                                    
cgccgGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAg                                                                                                                                                 >  3:143368/1‑149 (MQ=255)
cgccgGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAg                                                                                                                                                 <  3:754765/149‑1 (MQ=255)
  ccgGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCt                                                                                                                                               <  3:590321/149‑1 (MQ=255)
          gttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTtcgt                                                                                                                                       <  1:198582/149‑1 (MQ=255)
               cGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGcc                                                                                                                                  <  3:585863/149‑1 (MQ=255)
                 ccAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGccac                                                                                                                                >  4:296950/1‑149 (MQ=255)
                   aGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGccacca                                                                                                                              >  4:713381/1‑149 (MQ=255)
                   aGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGccacca                                                                                                                              >  2:208066/1‑149 (MQ=255)
                     gAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGa                                                                                                                            >  2:415068/1‑149 (MQ=255)
                      aaaCCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGaa                                                                                                                           <  2:193213/149‑1 (MQ=255)
                       aaCCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAAt                                                                                                                          >  2:652385/1‑149 (MQ=255)
                        aCCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCAGTCTTCGTCGGCCACCAGAATg                                                                                                                         <  2:225691/149‑1 (MQ=255)
                          cAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGt                                                                                                                       >  1:722207/1‑149 (MQ=255)
                           aGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTg                                                                                                                      >  2:399744/1‑149 (MQ=255)
                                tGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTc                                                                                                                 <  1:651388/149‑1 (MQ=255)
                                   aCCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCaaa                                                                                                              <  3:184507/149‑1 (MQ=255)
                                         gAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGaa                                                                                                                           >  1:506469/1‑130 (MQ=255)
                                         gAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGaa                                                                                                                           <  2:506469/130‑1 (MQ=255)
                                           atatTTCACCTCGGCGTTTTTTTGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGGCGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCgg                                                                                                      >  3:361985/1‑149 (MQ=255)
                                           atatTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGGCGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCgg                                                                                                      >  1:816857/1‑149 (MQ=255)
                                               ttCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGtttt                                                                                                  <  2:236759/149‑1 (MQ=255)
                                               ttCACCTCGGCGTTTTTATGGATGATGACTTCCACCACAGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGtttt                                                                                                  <  4:61350/149‑1 (MQ=255)
                                                   ccTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCt                                                                                              <  1:755399/149‑1 (MQ=255)
                                                    cTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTg                                                                                             <  2:191552/149‑1 (MQ=255)
                                                     tCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCt                                                                                              <  4:587530/147‑1 (MQ=255)
                                                     tCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCt                                                                                              >  3:587530/1‑147 (MQ=255)
                                                     tCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGc                                                                                            >  3:315216/1‑149 (MQ=255)
                                                     tCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGc                                                                                            <  1:475323/149‑1 (MQ=255)
                                                       ggCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGt                                                                                          <  3:320981/149‑1 (MQ=255)
                                                           tttttATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAAt                                                                                      >  3:171923/1‑149 (MQ=255)
                                                           tttttATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAAt                                                                                      <  2:621305/149‑1 (MQ=255)
                                                            ttttATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATg                                                                                     <  1:399744/149‑1 (MQ=255)
                                                            ttttATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATg                                                                                     >  2:512/1‑149 (MQ=255)
                                                                      gatgaCTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAgg                                                                           >  3:322674/1‑149 (MQ=255)
                                                                      gatgaCTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAgg                                                                           <  3:746120/149‑1 (MQ=255)
                                                                       atgaCTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGt                                                                          <  2:491149/149‑1 (MQ=255)
                                                                       atgaCTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGt                                                                          <  1:652385/149‑1 (MQ=255)
                                                                        tgaCTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGt                                                                          >  4:10380/1‑148 (MQ=255)
                                                                        tgaCTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGt                                                                          <  3:10380/148‑1 (MQ=255)
                                                                            ttCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGGCGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGaaa                                                                     <  4:361985/149‑1 (MQ=255)
                                                                            ttCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGGCGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGaaa                                                                     <  2:816857/149‑1 (MQ=255)
                                                                            ttCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGaaa                                                                     >  1:599710/1‑149 (MQ=255)
                                                                            ttCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGaaa                                                                     <  3:713381/149‑1 (MQ=255)
                                                                                accacTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTc                                                                 <  2:599710/149‑1 (MQ=255)
                                                                                accacTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTc                                                                 >  3:43325/1‑149 (MQ=255)
                                                                                accacTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTc                                                                 >  3:465875/1‑149 (MQ=255)
                                                                                  cacTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGccacca                                                                                                                              <  2:669208/86‑1 (MQ=255)
                                                                                  cacTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGccacca                                                                                                                              >  1:669208/1‑86 (MQ=255)
                                                                                         gcgTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCa                                                        <  3:296950/149‑1 (MQ=255)
                                                                                               aaCTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCAc                                                  >  1:261082/1‑149 (MQ=255)
                                                                                                  tGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGcc                                               >  4:448446/1‑149 (MQ=255)
                                                                                                    aTAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCtt                                             >  1:72152/1‑149 (MQ=255)
                                                                                                      aGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTa                                           >  2:118970/1‑149 (MQ=255)
                                                                                                      aGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTa                                           >  1:490010/1‑149 (MQ=255)
                                                                                                          gTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGca                                       <  1:208066/149‑1 (MQ=255)
                                                                                                            cACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGcaca                                     <  4:171923/149‑1 (MQ=255)
                                                                                                             aCGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACat                                    >  2:329165/1‑149 (MQ=255)
                                                                                                                 aCCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACataaat                                >  3:774535/1‑149 (MQ=255)
                                                                                                                  ccGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACataaata                               <  1:329165/149‑1 (MQ=255)
                                                                                                                   cGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACataaataa                              <  4:143368/149‑1 (MQ=255)
                                                                                                                    ggggCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACAtaaataaa                             <  1:512/146‑1 (MQ=255)
                                                                                                                    ggAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACAtaaataaa                             <  4:315216/149‑1 (MQ=255)
                                                                                                                    ggAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACAtaaataaa                             <  4:322674/149‑1 (MQ=255)
                                                                                                                       gCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGt                          <  3:776354/149‑1 (MQ=255)
                                                                                                                           aaCAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCa                      >  1:750592/1‑149 (MQ=255)
                                                                                                                                ccTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCCTCaga                 <  4:43325/149‑1 (MQ=255)
                                                                                                                                ccTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCaga                 >  4:535412/1‑149 (MQ=255)
                                                                                                                                 cTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCagag                >  1:675355/1‑149 (MQ=255)
                                                                                                                                 cTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCagag                >  4:363584/1‑149 (MQ=255)
                                                                                                                                  ttCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGaaaa                                                                               >  1:456105/1‑85 (MQ=255)
                                                                                                                                  ttCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGaaaa                                                                               <  2:456105/85‑1 (MQ=255)
                                                                                                                                  ttCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAgg               >  3:736743/1‑149 (MQ=255)
                                                                                                                                      aTGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTAc           >  4:644549/1‑149 (MQ=255)
                                                                                                                                       tGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTAcc          >  3:138656/1‑149 (MQ=255)
                                                                                                                                               aCGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCAtt  <  3:363584/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGCCGCGTGTAACTGATAGCTGTCACGCACCGGAGCGGAACAGCCTTCAATGTAGCTGACGTAGCTGTCTTCGTCGGCCACCAGAATGGTGCGCTCAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATT  >  NC_000913/1763124‑1763415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: