Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,193,709 T→A 5.2% M154L (ATG→TTG)  mrp ← antiporter inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,193,7090TA 5.2% 252.1 / 2.4 75M154L (ATG→TTG) mrpantiporter inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (38/32);  new base A (2/2);  total (41/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTCGCCATCGGTCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTT  >  NC_000913/2193565‑2193850
                                                                                                                                                |                                                                                                                                             
tggtggCCGTCGGTCCACGCCACACCATCGGATTGTCGTCAGTGACCAGATAACCGATTGAATTGTTTGCCAGGCCATGAGAGATAATCGGTGCCATAAGGGTACCGTCCGGCGAAGTTTTACGCTGATTTTCTGCTCCCAACgttgtt                                                                                                                                           <  4:342232/144‑1 (MQ=255)
 gCTCGCCATCGGTCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACAttgttg                                                                                                                                          <  4:112401/149‑1 (MQ=255)
 gCTCGCCATCGGTCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACAttgttg                                                                                                                                          <  1:746019/149‑1 (MQ=255)
     gCCATCGGTCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGAt                                                                                                                                      >  2:117800/1‑149 (MQ=255)
          cGGTCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATg                                                                                                                                 <  1:772011/149‑1 (MQ=255)
           ggTCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATgg                                                                                                                                <  3:769599/149‑1 (MQ=255)
               cACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCg                                                                                                                            >  2:620698/1‑149 (MQ=255)
                aCGCCACACCATCGCATTGTCGTCAGCGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGt                                                                                                                           >  1:349450/1‑149 (MQ=255)
                 cGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTa                                                                                                                          <  1:713840/149‑1 (MQ=255)
                    cgcacCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGat                                                                                                                       >  3:510183/3‑149 (MQ=255)
                      cacCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGatat                                                                                                                     <  4:362799/149‑1 (MQ=255)
                       acCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATgg                                                                                                                                >  1:222649/1‑137 (MQ=255)
                       acCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATgg                                                                                                                                <  2:222649/137‑1 (MQ=255)
                               aTTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCa                                                                                                            >  3:552270/1‑149 (MQ=255)
                                      tcAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGcc                                                                                                     <  3:487198/149‑1 (MQ=255)
                                      tcAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGcc                                                                                                     >  2:35215/1‑149 (MQ=255)
                                        aGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGa                                                                                                   <  1:117800/149‑1 (MQ=255)
                                         gTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGAc                                                                                                  >  2:62781/1‑149 (MQ=255)
                                         gTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGAc                                                                                                  >  2:718384/1‑149 (MQ=255)
                                          tGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATggg                                                                                                                               >  1:515194/1‑119 (MQ=255)
                                          tGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATggg                                                                                                                               <  2:515194/119‑1 (MQ=255)
                                            aCCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACttt                                                                                               >  3:144096/1‑149 (MQ=255)
                                             ccAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTc                                                                                              <  1:620698/149‑1 (MQ=255)
                                             ccAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTc                                                                                              <  4:765499/149‑1 (MQ=255)
                                              cAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCg                                                                                             >  4:166555/1‑149 (MQ=255)
                                                gATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGcc                                                                                           <  2:345910/149‑1 (MQ=255)
                                                   aaCCAATTCAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCt                                                                                        >  4:514916/1‑149 (MQ=255)
                                                       aattgaattgGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGGCGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATc                                                                                                                    >  3:362447/1‑117 (MQ=255)
                                                       aattgaattgGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGGCGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATc                                                                                                                    <  4:362447/117‑1 (MQ=255)
                                                       aattgaattgGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATc                                                                                                                    <  4:166998/117‑1 (MQ=255)
                                                       aattgaattgGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACACTGTTGGGATTGATGGGCCGTAGATATc                                                                                                                    >  3:166998/1‑117 (MQ=255)
                                                       aattgaattgGTTGCCAGGCCATGAGACATAATCGCTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATc                                                                                                                    <  2:238534/117‑1 (MQ=255)
                                                       aattgaattgGTTGCCAGGCCATGAGACATAATCGCTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGACGGGCCGTAGATATc                                                                                                                    >  1:238534/1‑117 (MQ=255)
                                                        attgaattgGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCg                                                                                                                   <  4:552117/117‑1 (MQ=255)
                                                        attgaattgGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCg                                                                                                                   >  3:552117/1‑117 (MQ=255)
                                                        attgaattgGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTgc                                                                                   <  1:718384/149‑1 (MQ=255)
                                                        attgaattgGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTgc                                                                                   <  1:62781/149‑1 (MQ=255)
                                                         ttgaattgGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTgcc                                                                                  >  4:763204/1‑149 (MQ=255)
                                                                   tGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCcgccagcgcca                                                                        >  3:617461/1‑149 (MQ=255)
                                                                       aGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGc                                                                    <  1:156141/149‑1 (MQ=255)
                                                                       aGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGc                                                                    >  1:458550/1‑149 (MQ=255)
                                                                        ggCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGcc                                                                   >  3:59760/1‑149 (MQ=255)
                                                                          ccATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAg                                                                 >  1:787255/1‑149 (MQ=255)
                                                                          ccATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAg                                                                 <  2:707579/149‑1 (MQ=255)
                                                                           cATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAgg                                                                <  1:35215/149‑1 (MQ=255)
                                                                           cATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAgg                                                                <  4:617461/149‑1 (MQ=255)
                                                                           cATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAgg                                                                >  2:645751/1‑149 (MQ=255)
                                                                             tGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGtt                                                              <  1:645751/149‑1 (MQ=255)
                                                                             tGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGtt                                                              <  3:763204/149‑1 (MQ=255)
                                                                                    tAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTAccgcc                                                       >  3:177157/1‑149 (MQ=255)
                                                                                     aaTCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTAccgccg                                                      >  3:83924/1‑149 (MQ=255)
                                                                                               atatGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGAttt                                            >  4:379616/1‑149 (MQ=255)
                                                                                               atatGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGAttt                                            >  3:9047/1‑149 (MQ=255)
                                                                                               atatGGGTACCGTCCGGCGAAGTTGGACGCAGATTTTCTGCGCCCAACATTGTTGGGAGTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGAttt                                            >  1:554745/1‑149 (MQ=255)
                                                                                                tatGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTa                                           <  4:479291/149‑1 (MQ=255)
                                                                                                 atGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTAc                                          <  3:332178/149‑1 (MQ=255)
                                                                                                      tACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCAcg                                     >  3:193959/1‑149 (MQ=255)
                                                                                                        ccGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAg                                                                 <  3:617834/119‑1 (MQ=255)
                                                                                                        ccGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAg                                                                 <  3:403899/119‑1 (MQ=255)
                                                                                                        ccGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAg                                                                 >  4:403899/1‑119 (MQ=255)
                                                                                                        ccGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAg                                                                 >  4:617834/1‑119 (MQ=255)
                                                                                                          gTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCg                                                                                                                   <  3:492024/67‑1 (MQ=255)
                                                                                                          gTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCg                                                                                                                   >  4:492024/1‑67 (MQ=255)
                                                                                                              ggCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCttt                             >  2:573747/1‑149 (MQ=255)
                                                                                                              ggCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCttt                             >  4:631088/1‑149 (MQ=255)
                                                                                                                     ttGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGaa                      <  4:552270/149‑1 (MQ=255)
                                                                                                                           gCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCAcc                >  2:43802/1‑149 (MQ=255)
                                                                                                                            cTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACcg               >  4:127776/1‑149 (MQ=255)
                                                                                                                            cTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACcg               >  3:281369/1‑149 (MQ=255)
                                                                                                                             tGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACcgc              >  3:523693/1‑149 (MQ=255)
                                                                                                                                     tGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAAt        <  1:167930/147‑1 (MQ=255)
                                                                                                                                     tGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAAt        >  2:167930/1‑147 (MQ=255)
                                                                                                                                      gcgcCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGtt     <  4:243401/149‑1 (MQ=255)
                                                                                                                                       atcccAACAATGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGa                                               <  2:274425/104‑1 (MQ=255)
                                                                                                                                       atcccAACAATGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGa                                               >  1:274425/3‑106 (MQ=255)
                                                                                                                                       atcccAACAATGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAAt        <  4:51665/143‑1 (MQ=255)
                                                                                                                                       atcccAACAATGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAAt        >  3:51665/3‑145 (MQ=255)
                                                                                                                                         cccAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGttctt  <  3:514916/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
TGCTCGCCATCGGTCCACGCCACACCATCGCATTGTCGTCAGTGACCAGATAACCAATTGAATTGGTTGCCAGGCCATGAGACATAATCGGTGCCATATGGGTACCGTCCGGCGAAGTTGGACGCTGATTTTCTGCGCCCAACATTGTTGGGATTGATGGGCCGTAGATATCGGCGTCCAGAATGCCGACTTTCGCCCCTTCTGCCGCCAGCGCCAGTGCCAGGTTTACCGCCGTGGACGATTTACCCACGCCGCCTTTACCGGAACTCACCGCGATAATGTTCTT  >  NC_000913/2193565‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: