Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,359,750 G→T 5.8% Q135K (CAG→AAG)  rhmT ← putative L‑rhamnonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,359,7500GT 5.8% 297.8 / 5.5 83Q135K (CAG→AAG) rhmTputative L‑rhamnonate transporter
Reads supporting (aligned to +/- strand):  ref base G (37/41);  new base T (3/2);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CTTCAATCACAAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCG  >  NC_000913/2359606‑2359896
                                                                                                                                                |                                                                                                                                                  
cTTCAATCACAAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTTCGGa                                                                                                                                                >  1:587276/1‑149 (MQ=255)
cTTCAATCACAAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTTCGGa                                                                                                                                                >  1:405975/1‑149 (MQ=255)
 ttCAATCACAAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGaa                                                                                                                                               >  2:753428/1‑149 (MQ=255)
 ttCAATCACAAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGaa                                                                                                                                               >  2:475000/1‑149 (MQ=255)
 ttCAATCACAAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCCCGATTACGCTTCGGaa                                                                                                                                               >  2:303788/1‑149 (MQ=255)
     aTCACAAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCa                                                                                                                                           >  2:348429/1‑149 (MQ=255)
      tCACAAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCAt                                                                                                                                          >  4:218732/1‑149 (MQ=255)
        acaAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTg                                                                                                                                        >  3:248419/1‑149 (MQ=255)
          aaaCATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCg                                                                                                                                      >  1:537165/1‑149 (MQ=255)
          aaaCATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCg                                                                                                                                      >  3:593484/1‑149 (MQ=255)
            aCATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAg                                                                                                                                    >  3:116998/1‑149 (MQ=255)
            aCATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAg                                                                                                                                    <  4:455483/149‑1 (MQ=255)
            aCATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAg                                                                                                                                    <  1:738143/149‑1 (MQ=255)
            aCATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAg                                                                                                                                    <  4:320350/149‑1 (MQ=255)
             cATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAgg                                                                                                                                   >  3:666448/1‑149 (MQ=255)
              aTCCAGAACCGGCCGGAAGGCCCGATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGATCACCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGt                                                                                                                                  <  4:557167/149‑1 (MQ=255)
               accAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTg                                                                                                                                 >  1:580909/2‑149 (MQ=255)
                ccAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTga                                                                                                                                <  2:135322/149‑1 (MQ=255)
                ccAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTga                                                                                                                                >  2:118344/1‑149 (MQ=255)
                  aGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAAc                                                                                                                                             <  1:484593/134‑1 (MQ=255)
                  aGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAAc                                                                                                                                             >  2:664036/1‑134 (MQ=255)
                  aGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAAc                                                                                                                                             >  2:484593/1‑134 (MQ=255)
                  aGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAAc                                                                                                                                             <  1:664036/134‑1 (MQ=255)
                  aGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTgaga                                                                                                                              >  4:314086/1‑149 (MQ=255)
                   gAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGAt                                                                                                                             >  1:690381/1‑149 (MQ=255)
                      ccAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATaaa                                                                                                                          <  4:744336/149‑1 (MQ=255)
                       cAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAAt                                                                                                                         <  3:218732/149‑1 (MQ=255)
                       cAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAAt                                                                                                                         >  3:211893/1‑149 (MQ=255)
                       cAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAAt                                                                                                                         <  4:666448/149‑1 (MQ=255)
                       cAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAAt                                                                                                                         >  2:170032/1‑149 (MQ=255)
                        aGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATc                                                                                                                        <  4:593484/149‑1 (MQ=255)
                         gCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCTCGATGACGCTGCGGAAACCATTGCGAGGTGAGATAAATCa                                                                                                                       >  4:520368/1‑149 (MQ=255)
                         gCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGt                                                                                                                                  >  3:238096/1‑138 (MQ=255)
                         gCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGt                                                                                                                                  <  4:238096/138‑1 (MQ=255)
                            gggATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATAc                                                                                                                    <  1:758111/149‑1 (MQ=255)
                              gATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCa                                                                                                                  >  4:199770/1‑149 (MQ=255)
                              gATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCa                                                                                                                  <  3:130280/149‑1 (MQ=255)
                                tGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAgg                                                                                                                <  3:154592/149‑1 (MQ=255)
                                 gCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAggg                                                                                                               <  4:444621/149‑1 (MQ=255)
                                    ccATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGaaa                                                                                                            >  2:76618/1‑149 (MQ=255)
                                     cATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGaaaa                                                                                                           >  4:12643/1‑149 (MQ=255)
                                         aaTCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTTCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATcc                                                                                                       <  2:587276/149‑1 (MQ=255)
                                         aaTCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTTCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATcc                                                                                                       <  2:405975/149‑1 (MQ=255)
                                          aTCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCg                                                                                                      <  2:22915/149‑1 (MQ=255)
                                           tCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCAt                                                                                                                                          <  1:213923/112‑1 (MQ=255)
                                           tCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCAt                                                                                                                                          >  2:213923/1‑112 (MQ=255)
                                            ccATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGc                                                                                                    >  4:116092/1‑149 (MQ=255)
                                                  aTCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCgc                                                                                              >  4:155372/1‑149 (MQ=255)
                                                   tctcCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGTGATAAATCATACCAg                                                                                                                 <  4:592191/129‑1 (MQ=255)
                                                   tctcCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAg                                                                                                                 >  3:592191/1‑129 (MQ=255)
                                                   tctcCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCgca                                                                                             >  2:738094/1‑149 (MQ=255)
                                                      ccAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCgcagca                                                                                          >  3:231891/1‑149 (MQ=255)
                                                      ccAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCgcagca                                                                                          >  3:422292/1‑149 (MQ=255)
                                                      ccAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCgcagca                                                                                          <  1:76618/149‑1 (MQ=255)
                                                       cAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCAc                                                                                         >  3:711637/1‑149 (MQ=255)
                                                               cgcCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCaga                                                                                 <  3:520368/149‑1 (MQ=255)
                                                                   ggAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGc                                                                             <  3:199770/149‑1 (MQ=255)
                                                                    gAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTCCg                                                                            <  4:711637/149‑1 (MQ=255)
                                                                           gTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATc                                                                     <  3:155372/149‑1 (MQ=255)
                                                                            tGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCa                                                                    <  4:231891/149‑1 (MQ=255)
                                                                                     tgtTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCg                                                           <  2:400693/149‑1 (MQ=255)
                                                                                      gtTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGc                                                          <  1:348429/149‑1 (MQ=255)
                                                                                        tAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCtt                                                        <  2:580909/149‑1 (MQ=255)
                                                                                                    tGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGccc                                            <  2:636985/149‑1 (MQ=255)
                                                                                                           atatAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCccatgccat                                     <  2:169293/149‑1 (MQ=255)
                                                                                                             ataGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCaga                                                                                 <  1:15253/103‑1 (MQ=255)
                                                                                                             ataGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCaga                                                                                 >  2:15253/1‑103 (MQ=255)
                                                                                                             ataGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATgg                                   >  2:208642/1‑149 (MQ=255)
                                                                                                              taGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGc                                  <  4:211893/149‑1 (MQ=255)
                                                                                                               aGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCt                                 <  4:422292/149‑1 (MQ=255)
                                                                                                                 aaCAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGc                               >  1:338975/1‑149 (MQ=255)
                                                                                                                      ccccATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCgaaa                          <  2:537165/149‑1 (MQ=255)
                                                                                                                        ccATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGTCCATGCCATGGCTGCCgaaaga                        >  3:491159/1‑149 (MQ=255)
                                                                                                                         cATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCgaaagaa                       <  3:585526/149‑1 (MQ=255)
                                                                                                                         cATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCgaaa                          >  2:188251/1‑146 (MQ=255)
                                                                                                                         cATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCgaaa                          <  1:188251/146‑1 (MQ=255)
                                                                                                                          aTAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCgaaagaaa                      >  3:271209/1‑149 (MQ=255)
                                                                                                                            aaTGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATc                    <  2:214900/149‑1 (MQ=255)
                                                                                                                             aTGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATcc                   <  1:303788/149‑1 (MQ=255)
                                                                                                                                  ggCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCcaca              <  2:690381/149‑1 (MQ=255)
                                                                                                                                    cgcgATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCAcagc            <  1:738094/149‑1 (MQ=255)
                                                                                                                                        aTTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAgtg        <  1:118344/149‑1 (MQ=255)
                                                                                                                                              cTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCg  >  1:230503/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CTTCAATCACAAACATCCAGAACCAGCCGGGATGCCCCATAAATCCATGCATCTCCAACAGCGCGCCGGAAAGCGGTGATCCCAGTGTTAACGCCAGCGGTGCGCCCATATAGAACAGCCCCATAATGCTGGCGCGATTACGCTGCGGAAACCATTGCGAGGTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCG  >  NC_000913/2359606‑2359896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: