Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,657,460 A→G 5.3% V492V (GTT→GTC hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,657,4600AG 5.3% 253.0 / 3.3 76V492V (GTT→GTChscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base A (46/26);  new base G (2/2);  total (48/28)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

AGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCA  >  NC_000913/2657317‑2657605
                                                                                                                                               |                                                                                                                                                 
aGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGcc                                                                                                                                              >  2:440008/1‑149 (MQ=255)
 gCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCg                                                                                                                                             <  4:462234/149‑1 (MQ=255)
       gcgcCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGAt                                                                                                                                       <  1:264032/149‑1 (MQ=255)
         gccgcTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGAttt                                                                                                                                     >  1:363570/1‑149 (MQ=255)
            gcTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATGGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTtctc                                                                                                                                  >  2:236193/1‑149 (MQ=255)
            gcTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTtctc                                                                                                                                  <  2:214129/149‑1 (MQ=255)
                 tACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCg                                                                                                                             >  3:332972/1‑149 (MQ=255)
                 tACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGGCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCg                                                                                                                             >  3:91607/1‑149 (MQ=255)
                       ttGTTCTGCCAGCTTTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCa                                                                                                                       <  4:484006/149‑1 (MQ=255)
                               ccAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCaaa                                                                                                               >  1:337682/1‑149 (MQ=255)
                                 aGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTc                                                                                                                        >  3:640393/1‑138 (MQ=255)
                                 aGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTc                                                                                                                        <  4:640393/138‑1 (MQ=255)
                                 aGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAg                                                                                                             >  2:642628/1‑149 (MQ=255)
                                 aGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAg                                                                                                             >  3:178319/1‑149 (MQ=255)
                                       cGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTc                                                                                                       <  2:565103/149‑1 (MQ=255)
                                        gggCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCg                                                                                                      >  4:319065/1‑149 (MQ=255)
                                        gggCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCGCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCg                                                                                                      >  3:502628/1‑149 (MQ=255)
                                         ggCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGc                                                                                                                            >  4:186055/1‑126 (MQ=255)
                                         ggCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGc                                                                                                                            <  3:186055/126‑1 (MQ=255)
                                                  gTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCGACGCCGGTGGATTTCTCCATc                                                                                                                              >  1:613048/1‑115 (MQ=255)
                                                  gTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCGACGCCGGTGGATTTCTCCATc                                                                                                                              <  2:115684/115‑1 (MQ=255)
                                                  gTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCGACGCCGGTGGATTTCTCCATc                                                                                                                              <  2:613048/115‑1 (MQ=255)
                                                  gTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCGACGCCGGTGGATTTCTCCATc                                                                                                                              >  1:115684/1‑115 (MQ=255)
                                                   tCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTg                                                                                           <  1:768559/149‑1 (MQ=255)
                                                             cATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCAcg                                                                                 >  2:242178/1‑149 (MQ=255)
                                                                 gCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGaa                                                                             >  2:767190/1‑149 (MQ=255)
                                                                   tCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAata                                                                           >  2:191302/1‑149 (MQ=255)
                                                                     aTTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGTTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATg                                                                         >  4:757696/1‑149 (MQ=255)
                                                                     aTTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATg                                                                         <  4:178319/149‑1 (MQ=255)
                                                                             tttGATCATCGAAGCTATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGt                                                                                                                                           <  4:674741/75‑1 (MQ=255)
                                                                             tttGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGt                                                                                                                                           >  3:674741/1‑75 (MQ=255)
                                                                                 atcatcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCt                                                                                            >  3:311336/1‑118 (MQ=255)
                                                                                 atcatcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCt                                                                                            <  4:311336/118‑1 (MQ=255)
                                                                                     tcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGt                                                         >  4:172844/1‑149 (MQ=255)
                                                                                       gAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGCCCGTCCCGCGAATATGCGCACCGCCAGCCGGTAg                                                       >  4:98024/1‑149 (MQ=255)
                                                                                       gAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAg                                                       >  2:603500/1‑149 (MQ=255)
                                                                                        aaGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAgc                                                      >  1:125323/1‑149 (MQ=255)
                                                                                        aaGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAgc                                                      >  1:777461/1‑149 (MQ=255)
                                                                                           cGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGcc                                                   <  4:390731/149‑1 (MQ=255)
                                                                                            gATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCg                                                  >  1:384803/1‑149 (MQ=255)
                                                                                            gATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCg                                                  <  1:440008/149‑1 (MQ=255)
                                                                                                tCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAAt                                              <  2:363570/149‑1 (MQ=255)
                                                                                                tCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAAt                                              <  1:603500/149‑1 (MQ=255)
                                                                                                 cGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCt                                                                                            <  1:541177/102‑1 (MQ=255)
                                                                                                 cGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCt                                                                                            >  4:254629/1‑102 (MQ=255)
                                                                                                 cGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCt                                                                                            <  3:254629/102‑1 (MQ=255)
                                                                                                 cGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCt                                                                                            >  2:541177/1‑102 (MQ=255)
                                                                                                    tATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCa                                          >  3:398897/1‑149 (MQ=255)
                                                                                                    tATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCa                                          >  2:265714/1‑149 (MQ=255)
                                                                                                     aTCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCAc                                         <  2:230734/149‑1 (MQ=255)
                                                                                                       cGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGc                                       >  3:443380/1‑149 (MQ=255)
                                                                                                       cGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGc                                       >  1:444022/1‑149 (MQ=255)
                                                                                                        ggTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCa                                      >  4:94535/1‑149 (MQ=255)
                                                                                                        ggTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCa                                      <  2:337682/149‑1 (MQ=255)
                                                                                                         gTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAg                                     >  1:361591/1‑149 (MQ=255)
                                                                                                            aGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGAAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAgcgc                                  >  3:318442/1‑149 (MQ=255)
                                                                                                               ccGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCaaa                               >  2:68089/1‑149 (MQ=255)
                                                                                                                     gACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACgcgc                         >  2:103337/1‑149 (MQ=255)
                                                                                                                      aCGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGcc                        >  1:691131/1‑149 (MQ=255)
                                                                                                                       cGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCa                       >  1:437290/1‑149 (MQ=255)
                                                                                                                         gTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGt                     <  1:242178/149‑1 (MQ=255)
                                                                                                                          tttGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTg                    >  2:276001/1‑149 (MQ=255)
                                                                                                                                cTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGc              <  4:332972/149‑1 (MQ=255)
                                                                                                                                cTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACAGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCAAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGc              <  4:91607/149‑1 (MQ=255)
                                                                                                                                cTGAATAGACGCCTAAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGc              >  1:356065/1‑149 (MQ=255)
                                                                                                                                  gAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAg            >  4:717996/1‑149 (MQ=255)
                                                                                                                                  gAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAg            >  1:58747/1‑149 (MQ=255)
                                                                                                                                   aaTAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGAcc                                                                                             >  1:189493/1‑67 (MQ=255)
                                                                                                                                   aaTAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGAcc                                                                                             <  2:189493/67‑1 (MQ=255)
                                                                                                                                   aaTAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGt           >  1:714800/1‑149 (MQ=255)
                                                                                                                                       gACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTg       >  1:353375/1‑149 (MQ=255)
                                                                                                                                        aCGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGc      <  3:94535/149‑1 (MQ=255)
                                                                                                                                          gCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGcc                                                                >  1:514924/1‑89 (MQ=255)
                                                                                                                                          gCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGcc                                                                <  2:514924/89‑1 (MQ=255)
                                                                                                                                           ccTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCAcc   >  4:686760/1‑149 (MQ=255)
                                                                                                                                            cTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGAAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCa  <  4:318442/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
AGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCA  >  NC_000913/2657317‑2657605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: