Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,663,474 A→T 5.5% A11A (GCA→GCT)  suhB → inositol monophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,663,4740AT 5.5% 322.1 / 7.7 91A11A (GCA→GCT) suhBinositol monophosphatase
Reads supporting (aligned to +/- strand):  ref base A (41/45);  new base T (2/3);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

TCTTTCTTCATCTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGATTCGTAAATCTTACCCAC  >  NC_000913/2663326‑2663622
                                                                                                                                                    |                                                                                                                                                    
tctttcttCATCTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcgcgca                                                                                                                                                      <  1:746405/149‑1 (MQ=255)
   ttcttcATCTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcgcgcagcg                                                                                                                                                   >  2:714723/1‑149 (MQ=255)
    tcttcATCTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcgcgcagcgc                                                                                                                                                  <  1:59628/149‑1 (MQ=255)
    tcttcATCTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTgcgcgcagcgc                                                                                                                                                  <  2:297247/149‑1 (MQ=255)
           cTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGTATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGc                                                                                                                                           >  2:339502/1‑149 (MQ=255)
           cTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGc                                                                                                                                           <  2:718412/149‑1 (MQ=255)
           cTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGc                                                                                                                                           <  4:300599/149‑1 (MQ=255)
            tAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCg                                                                                                                                          >  3:713720/1‑149 (MQ=255)
            tAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCg                                                                                                                                          >  2:395879/1‑149 (MQ=255)
             aaaGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTGCGCGCTGCGCGCACGGCga                                                                                                                                         <  1:83105/149‑2 (MQ=255)
                gATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGta                                                                                                                                      >  4:235874/1‑149 (MQ=255)
                  ttattCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaat                                                                                                                                    <  1:395879/149‑1 (MQ=255)
                       cACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaat                                                                                                                               <  4:665432/149‑1 (MQ=255)
                       cACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaat                                                                                                                               <  2:352266/149‑1 (MQ=255)
                       cACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaac                                                                                                                               <  4:713720/149‑2 (MQ=255)
                        aCGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaatt                                                                                                                              <  2:261575/149‑1 (MQ=255)
                        aCGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaatt                                                                                                                              >  2:291709/1‑149 (MQ=255)
                        aCGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaatt                                                                                                                              >  4:312600/1‑149 (MQ=255)
                         cGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTg                                                                                                                             >  2:457957/1‑149 (MQ=255)
                          gCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGc                                                                                                                            >  1:424637/1‑149 (MQ=255)
                          gCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGc                                                                                                                            <  1:785490/149‑1 (MQ=255)
                               ttATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCaaaa                                                                                                                       >  4:324677/1‑149 (MQ=255)
                                    aTAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTAt                                                                                                                  >  4:243964/1‑149 (MQ=255)
                                    aTAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTAt                                                                                                                  <  4:124009/149‑1 (MQ=255)
                                    aTAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTAt                                                                                                                  <  1:91653/149‑1 (MQ=255)
                                     tAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATg                                                                                                                 <  3:324677/149‑1 (MQ=255)
                                      aaaaCGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGa                                                                                                                >  2:79261/1‑149 (MQ=255)
                                        aaCGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGaaa                                                                                                              >  3:707428/1‑149 (MQ=255)
                                        aaCGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGaaa                                                                                                              <  3:588950/149‑1 (MQ=255)
                                        aaCGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGaaa                                                                                                              <  3:555896/149‑1 (MQ=255)
                                                 aGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACg                                                                                                     >  2:574783/1‑149 (MQ=255)
                                                 aGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACg                                                                                                     >  1:123474/1‑149 (MQ=255)
                                                    tGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaatt                                                                                                                              <  2:73114/121‑1 (MQ=255)
                                                    tGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaatt                                                                                                                              >  1:73114/1‑121 (MQ=255)
                                                         aTCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGaa                                                                                             <  1:457957/149‑1 (MQ=255)
                                                         aTCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGaa                                                                                             <  1:594276/149‑1 (MQ=255)
                                                           ctcGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGc                                                                                           <  2:123474/149‑1 (MQ=255)
                                                            tcGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaat                                                                                                                               >  2:105600/1‑112 (MQ=255)
                                                            tcGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGtaatttaat                                                                                                                               <  1:105600/112‑1 (MQ=255)
                                                                   tatcCTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTAt                                                                                                                  <  1:445854/118‑1 (MQ=255)
                                                                   tataCTCCGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTAt                                                                                                                  >  2:445854/1‑118 (MQ=255)
                                                                      aCTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAg                                                                                >  3:73997/1‑149 (MQ=255)
                                                                       ctctGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGTATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAgg                                                                               <  1:339502/149‑1 (MQ=255)
                                                                       ctctGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAgg                                                                               >  3:547440/1‑149 (MQ=255)
                                                                         ctGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCa                                                                             <  4:707428/149‑1 (MQ=255)
                                                                          tGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAg                                                                            <  3:312600/149‑1 (MQ=255)
                                                                          tGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAg                                                                            <  1:291709/149‑1 (MQ=255)
                                                                              ccGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAc                                                                        <  1:699712/149‑1 (MQ=255)
                                                                              ccGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAc                                                                        <  1:495996/149‑1 (MQ=255)
                                                                                gTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAc                                                                        >  3:629950/1‑147 (MQ=255)
                                                                                gTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAc                                                                        >  1:164241/1‑147 (MQ=255)
                                                                                gTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAc                                                                        <  2:164241/147‑1 (MQ=255)
                                                                                gTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAc                                                                        <  4:629950/147‑1 (MQ=255)
                                                                                gTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGa                                                                      <  3:442473/149‑1 (MQ=255)
                                                                                  tttCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGAtt                                                                    >  4:118137/1‑149 (MQ=255)
                                                                                   ttCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGAttt                                                                   <  1:287858/149‑1 (MQ=255)
                                                                                     cccGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCg                                                                 >  3:459822/1‑149 (MQ=255)
                                                                                      ccGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGt                                                                <  4:73997/149‑1 (MQ=255)
                                                                                      ccGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGt                                                                >  3:476238/1‑149 (MQ=255)
                                                                                       cGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTgcgcgctgcgcgcac                                                                                                                                              >  2:342081/1‑69 (MQ=39)
                                                                                       cGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCTTgcgcgctgcgcgcac                                                                                                                                              <  1:342081/70‑2 (MQ=39)
                                                                                            tttAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCaa                                                          >  4:764188/1‑149 (MQ=255)
                                                                                            tttAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCaa                                                          >  4:174898/1‑149 (MQ=255)
                                                                                               aaCATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGt                                                       >  4:679022/1‑149 (MQ=255)
                                                                                                  aTCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTa                                                      >  1:253568/1‑147 (MQ=255)
                                                                                                  aTCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTa                                                      <  2:253568/147‑1 (MQ=255)
                                                                                                  aTCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGa                                                    <  3:679022/149‑1 (MQ=255)
                                                                                                  aTCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGa                                                    >  3:97291/1‑149 (MQ=255)
                                                                                                  aTCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGa                                                    <  3:790268/149‑1 (MQ=255)
                                                                                                  aTCCAGTGAGAGAGACCGATGCAGCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGATCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGa                                                    >  1:727842/1‑149 (MQ=255)
                                                                                                    ccAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATa                                                  <  1:564085/149‑1 (MQ=255)
                                                                                                         gagagagaCCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCt                                             <  3:118137/149‑1 (MQ=255)
                                                                                                               gaCCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGaa                                       >  2:152739/1‑149 (MQ=255)
                                                                                                                 ccGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGc                                     <  1:152739/149‑1 (MQ=255)
                                                                                                                 ccGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGc                                     <  2:424637/149‑1 (MQ=255)
                                                                                                                      gCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGa                                <  3:764188/149‑1 (MQ=255)
                                                                                                                      gCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGa                                <  4:547440/149‑1 (MQ=255)
                                                                                                                      gCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGa                                <  3:235874/149‑1 (MQ=255)
                                                                                                                       acggcGATGTTCAGCATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGAt                               >  3:184218/5‑149 (MQ=255)
                                                                                                                         tCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTa                             >  2:351945/1‑149 (MQ=255)
                                                                                                                         tCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTa                             >  3:793081/1‑149 (MQ=255)
                                                                                                                         tCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTa                             >  3:391635/1‑149 (MQ=255)
                                                                                                                           cGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAGACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGa                                <  3:276615/144‑1 (MQ=255)
                                                                                                                           cGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGa                                >  4:276615/1‑144 (MQ=255)
                                                                                                                                cTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGacac                      >  2:593044/1‑149 (MQ=255)
                                                                                                                                  gAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGaca                       >  2:275195/1‑146 (MQ=255)
                                                                                                                                  gAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGaca                       <  1:275195/146‑1 (MQ=255)
                                                                                                                                  gAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGa                    >  3:41842/1‑149 (MQ=255)
                                                                                                                                  gAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGa                    <  3:243964/149‑1 (MQ=255)
                                                                                                                                  gAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAAAGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGa                    <  1:79261/149‑1 (MQ=255)
                                                                                                                                   aaCATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGAt                   <  4:459822/149‑1 (MQ=255)
                                                                                                                                       ccGCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTg                                 >  3:2133/2‑131 (MQ=255)
                                                                                                                                       ccGCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTg                                 <  4:2133/130‑1 (MQ=255)
                                                                                                                                         gCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGa                                >  4:45651/1‑130 (MQ=255)
                                                                                                                                         gCCTTGCGCGCTGCGCGCACGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGa                                <  3:45651/130‑1 (MQ=255)
                                                                                                                                         gCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGATTCGTaa             >  4:45638/1‑149 (MQ=255)
                                                                                                                                            gTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGATTCGTAAATc          >  4:729429/1‑149 (MQ=255)
                                                                                                                                                 cgcagcgcgcaAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGATTCGTAAATCTTAcc     <  3:733440/149‑1 (MQ=255)
                                                                                                                                                    agcgcacaAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGATTCGTAAATCTTACCcac  >  4:630922/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCTTTCTTCATCTAAAGATTATTCACGCATCTTATCATAAAACGAAGACAGATGCCGATCTCGCTGCTATACTCTGCGCCGTTTTCCCGTTCTTTAACATCCAGTGAGAGAGACCGATGCATCCGATGCTGAACATCGCCGTGCGCGCAGCGCGCAAGGCGGGTAATTTAATTGCCAAAAACTATGAAACCCCGGACGCTGTAGAAGCGAGCCAGAAAGGCAGTAACGATTTCGTGACCAACGTAGATAAAGCTGCCGAAGCGGTGATTATCGACACGATTCGTAAATCTTACCCAC  >  NC_000913/2663326‑2663622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑