Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,706,607 | A→C | 6.4% | L173R (CTC→CGC) ‡ | lepA ← | back‑translocating elongation factor EF4, GTPase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,706,607 | 0 | A | C | 6.4% | 266.5 / 10.9 | 78 | L173R (CTC→CGC) ‡ | lepA | back‑translocating elongation factor EF4, GTPase |
Reads supporting (aligned to +/- strand): ref base A (38/35); new base C (3/2); total (41/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01 |
TTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGA > NC_000913/2706459‑2706754 | tttGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCga < 2:647899/149‑1 (MQ=255) ttGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCgag < 2:536274/149‑1 (MQ=255) tCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAAc < 1:677850/149‑1 (MQ=255) cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc < 1:240841/149‑1 (MQ=255) cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc < 4:600330/149‑1 (MQ=255) cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc < 4:660527/149‑1 (MQ=255) ccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc > 2:464141/1‑149 (MQ=255) gCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGcaca > 1:470399/1‑149 (MQ=255) aTACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGc < 2:262571/149‑1 (MQ=255) ggATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGaa < 1:269012/149‑1 (MQ=255) ggATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGaa > 4:138886/1‑149 (MQ=255) gATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCCCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGacc > 4:470551/1‑147 (MQ=255) aGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcg > 4:642991/1‑149 (MQ=255) gTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCaa < 4:579511/126‑1 (MQ=255) gTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCaa > 3:579511/1‑126 (MQ=255) aaaCAACGCCCAGGTAGTTGTCGAACCATGGGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACg < 4:376978/110‑1 (MQ=255) aaaCAACGCCCAGGTAGTTGTCGAACCATGGGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACg > 3:376978/1‑110 (MQ=255) caacGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCga > 1:287821/1‑102 (MQ=255) caacGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCga < 2:287821/102‑1 (MQ=255) ccAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTg > 3:549741/1‑149 (MQ=255) cAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTgg > 3:321710/1‑149 (MQ=255) tAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTc < 3:138886/149‑1 (MQ=255) ttGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCTCCTTCCGGCAGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAt < 4:549741/149‑1 (MQ=255) ttGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAt < 2:519504/149‑1 (MQ=255) gTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGc < 4:321710/149‑1 (MQ=255) gAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCcga > 2:343871/1‑149 (MQ=255) aaCCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCcgac > 1:629495/1‑149 (MQ=255) aCCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTc > 2:699165/1‑110 (MQ=255) aCCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTc < 1:699165/110‑1 (MQ=255) aCCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCcgacg > 3:353715/1‑149 (MQ=255) aTGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGata > 3:717389/1‑149 (MQ=255) gAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGcc < 4:519393/140‑1 (MQ=255) gAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGcc > 3:519393/1‑140 (MQ=255) gAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATc > 2:790727/1‑149 (MQ=255) aGTCGATAATTAGTGCCTGCAACGGGCATTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCt < 4:241866/149‑1 (MQ=255) gTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc > 2:297670/1‑82 (MQ=255) gTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc < 1:297670/82‑1 (MQ=255) gATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc < 2:531498/120‑1 (MQ=255) gATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTc > 1:531498/1‑120 (MQ=255) aTAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAAt < 4:422583/149‑1 (MQ=255) tAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAAtt < 1:768352/149‑1 (MQ=255) tGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCg < 1:790727/149‑1 (MQ=255) cTGCAACCGCCCTTCCGGATCGCCTTCCTGCGGCGGCATGTCCCGCACCTGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTCGCGTCGACGCCGATGATATCTTCATTTTCTTCCGCCa > 4:606740/1‑149 (MQ=255) tGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCAc < 3:511924/149‑1 (MQ=255) tGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACATCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCAc > 2:503358/1‑149 (MQ=255) gCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGc < 1:49377/112‑1 (MQ=255) gCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGc > 2:49377/1‑112 (MQ=255) aCGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGt > 3:345453/1‑149 (MQ=255) gggCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTc < 1:82134/149‑1 (MQ=255) ttCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCg > 3:675762/1‑70 (MQ=255) ttCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCg < 4:675762/70‑1 (MQ=255) ttCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGAt > 3:484328/1‑149 (MQ=255) ttCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGAt > 3:741672/1‑149 (MQ=255) ccGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCg < 1:343871/149‑1 (MQ=255) gATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCt > 1:142137/1‑149 (MQ=255) gATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCt > 4:684007/1‑149 (MQ=255) aTCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTg > 2:227008/1‑149 (MQ=255) tCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGc > 1:385204/1‑149 (MQ=255) ttCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCa < 2:142137/149‑1 (MQ=255) ttCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCa < 1:85262/149‑1 (MQ=255) tCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAg < 1:582746/149‑1 (MQ=255) cggcggAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCaa < 2:3022/149‑1 (MQ=255) ggcggAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGTATCGGCTGCCGGCAGGTCAAt < 4:716866/149‑1 (MQ=255) aTGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt > 3:772841/1‑149 (MQ=255) tGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTc < 4:345453/149‑1 (MQ=255) gcgcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCg > 1:149519/1‑121 (MQ=255) gcgcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCg < 2:149519/121‑1 (MQ=255) gcgcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTa > 4:501815/1‑149 (MQ=255) cgcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTAc < 1:464141/149‑1 (MQ=255) ccAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGc < 3:606740/149‑1 (MQ=255) ccAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGc > 3:193971/1‑149 (MQ=255) cAGACGTTCGAGAACGTCCTGCGCACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGca > 1:491952/1‑149 (MQ=255) aggACGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATttct > 3:795310/3‑94 (MQ=255) aggACGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATttct < 4:795310/92‑1 (MQ=255) aggACGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGca > 1:465311/3‑149 (MQ=255) ggACGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATc < 3:658277/82‑1 (MQ=255) ggACGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATc > 4:658277/2‑83 (MQ=255) aGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGcac > 2:573760/1‑149 (MQ=255) gACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAg > 3:94753/1‑139 (MQ=255) gACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAg < 4:94753/139‑1 (MQ=255) ttCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACtt > 2:654917/1‑149 (MQ=255) ttCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACtt > 3:475148/1‑149 (MQ=255) gagaACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCga > 1:219511/1‑149 (MQ=255) | TTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGA > NC_000913/2706459‑2706754 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |