| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,715,260 | C→T | 11.3% | A21T (GCT→ACT) ‡ | yfiE ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,715,260 | 0 | C | T | 11.3% | 156.2 / 7.7 | 53 | A21T (GCT→ACT) ‡ | yfiE | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (29/18); new base T (2/4); total (31/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAA > NC_000913/2715114‑2715407 | aTAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑Tc > 1:267258/1‑149 (MQ=255) tgCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAGCT‑TCGCaaaaa < 2:162129/149‑1 (MQ=255) tgCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGACACCGTCGATTGTGTACAGCACAATTTTTGCGAGCT‑TCTCaaaaa < 4:238296/149‑1 (MQ=255) aaCTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCctc > 4:103739/1‑149 (MQ=255) ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTc > 3:796310/1‑149 (MQ=255) ttttttCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTc > 3:625552/1‑149 (MQ=255) ttCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACgg > 4:164213/1‑149 (MQ=255) ggTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTaa < 1:318592/149‑1 (MQ=255) gCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGaa > 2:717346/1‑121 (MQ=255) gCACATCCGTCGGACAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGaa < 1:717346/121‑1 (MQ=255) tCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATaaaa < 3:480758/149‑1 (MQ=255) cGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACg < 2:267258/149‑1 (MQ=255) tcAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCCGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAAc > 2:73447/1‑149 (MQ=255) gaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGTACAATTTTTGCGAAGC‑TCGCaaaaa > 4:693501/2‑93 (MQ=255) aaaTAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCaaaaa < 3:693501/93‑1 (MQ=255) tGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCt > 1:147253/1‑149 (MQ=255) ggACTGATAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGCCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTa < 3:688973/149‑1 (MQ=255) tGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGa > 3:488423/1‑149 (MQ=255) aaaCTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGaaaa < 4:796310/149‑1 (MQ=255) aaaCTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGaaaa < 3:423079/149‑1 (MQ=255) cTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAatat < 2:444256/149‑1 (MQ=255) cTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAatat < 3:164213/149‑1 (MQ=255) tCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCt > 4:303645/1‑149 (MQ=255) cAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTa < 3:303645/149‑1 (MQ=255) atatGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATaaaaa < 2:147253/149‑1 (MQ=255) tatGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAAc < 3:103739/149‑1 (MQ=255) tGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAAcat > 3:33380/1‑149 (MQ=255) aGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAAcattcatt > 1:657097/1‑149 (MQ=255) tCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCAtttt > 2:595902/1‑149 (MQ=255) ccGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCAttttttt > 3:678375/1‑149 (MQ=255) gTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTaa > 1:120226/1‑149 (MQ=255) gTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTaa > 2:499794/1‑149 (MQ=255) tCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACACTCATTTTTTTaac < 4:692972/149‑2 (MQ=255) aTTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGt > 2:545985/1‑149 (MQ=255) aTTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGt > 2:103471/1‑149 (MQ=255) ttGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAAc < 4:84761/90‑1 (MQ=255) ttGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAAc > 3:84761/1‑90 (MQ=255) gtACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCgt > 3:233752/1‑149 (MQ=255) tACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCgtg > 1:656404/1‑149 (MQ=255) aGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCg > 4:188893/1‑149 (MQ=255) gCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCgg > 2:649485/1‑149 (MQ=255) acaATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGt > 4:20196/1‑149 (MQ=255) ctttttGCGAGAT‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACt > 4:355075/2‑149 (MQ=255) ctttttGCG‑AGCTTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACt > 4:131757/2‑149 (MQ=255) tttttGCGAGCT‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATaaaaa < 3:795898/114‑1 (MQ=255) tttttGCGAGCT‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATaaaaa > 4:795898/1‑114 (MQ=255) tttttGCGAGCT‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCggg < 3:536352/144‑1 (MQ=255) tttttGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTg > 1:815298/1‑149 (MQ=255) tttttGCG‑AGCTTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCGAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCggg > 4:536352/1‑144 (MQ=255) gCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTAc < 1:73447/149‑1 (MQ=255) cGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTAcc > 1:537543/1‑149 (MQ=255) aGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCaaa < 4:488423/149‑1 (MQ=255) gC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCaaaa < 1:649485/149‑1 (MQ=255) | ATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGC‑TCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAA > NC_000913/2715114‑2715407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |