Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,750,086 A→C 6.8% intergenic (+26/+29) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,750,0860AC 6.8% 227.7 / 7.9 66intergenic (+26/+29)yfjD/grpEUPF0053 family inner membrane protein/heat shock protein
Reads supporting (aligned to +/- strand):  ref base A (27/34);  new base C (3/2);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCAC  >  NC_000913/2749943‑2750231
                                                                                                                                               |                                                                                                                                                 
aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATGCGAATGCCGGGcc                                                                                                                                              <  2:416815/149‑1 (MQ=255)
     cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGaa                                                                                                                                         <  3:159342/149‑1 (MQ=255)
     cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGaa                                                                                                                                         <  1:324894/149‑1 (MQ=255)
     cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGaa                                                                                                                                         <  3:514761/149‑1 (MQ=255)
      ccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAat                                                                                                                                        >  3:120102/1‑149 (MQ=255)
                aTTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGaaa                                                                                                                              <  1:138861/149‑1 (MQ=255)
                 ttGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAAttatta                                                                                                                                   <  1:258504/143‑1 (MQ=255)
                 ttGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAAttatta                                                                                                                                   >  2:258504/1‑143 (MQ=255)
                      gAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGaa                                                                                                                        >  4:65014/1‑149 (MQ=255)
                           cgatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTaa                                                                                                                   <  2:583766/149‑1 (MQ=255)
                            gatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTaa                                                                                                                   >  2:591914/1‑148 (MQ=255)
                            gatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTaa                                                                                                                   <  1:591914/148‑1 (MQ=255)
                            gatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAg                                                                                                                  >  1:518167/1‑149 (MQ=255)
                               atcgatatACTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAattatt                                                                                                                                    <  2:659008/128‑1 (MQ=255)
                               atcgatatACTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAattatt                                                                                                                                    >  1:659008/1‑128 (MQ=255)
                                 cgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCtttt                                                                                                             <  4:761269/149‑1 (MQ=255)
                                   atatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGc                                                                                                           <  4:120102/149‑1 (MQ=255)
                                      ttCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                                        <  4:136798/149‑1 (MQ=255)
                                      ttCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                                        <  1:268095/149‑1 (MQ=255)
                                         tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAtt                                                                                                                     <  4:19025/133‑1 (MQ=255)
                                         tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAtt                                                                                                                     >  3:19025/1‑133 (MQ=255)
                                         tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                          >  3:424164/1‑144 (MQ=255)
                                         tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                          <  4:424164/144‑1 (MQ=255)
                                         tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                     <  1:142147/149‑1 (MQ=255)
                                         tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                     >  2:142147/1‑149 (MQ=255)
                                              gTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                                                                                                >  2:349492/1‑149 (MQ=255)
                                                 cAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTaa                                                                                             >  4:376078/1‑149 (MQ=255)
                                                 cAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTaa                                                                                             >  4:503558/1‑149 (MQ=255)
                                                  aGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAg                                                                                                                                                  <  2:238894/95‑1 (MQ=255)
                                                  aGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAg                                                                                                                                                  >  1:238894/1‑95 (MQ=255)
                                                  aGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAc                                                                                            <  2:708516/149‑1 (MQ=255)
                                                    gACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCa                                                                                          >  2:193598/1‑149 (MQ=255)
                                                    gACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCa                                                                                          >  1:155540/1‑149 (MQ=255)
                                                       aaTATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcg                                                                                       >  3:8884/1‑149 (MQ=255)
                                                           tGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgccgc                                                                                   >  2:764087/1‑149 (MQ=255)
                                                                      gTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTAc                                                                        <  2:815634/149‑1 (MQ=255)
                                                                       tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATGCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACg                                                                       >  3:773959/1‑149 (MQ=255)
                                                                         aaaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAc                                                                     >  4:437781/1‑149 (MQ=255)
                                                                          aaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAcc                                                                    <  2:812186/149‑1 (MQ=255)
                                                                          aaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCAGGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAcc                                                                    <  4:773959/149‑1 (MQ=255)
                                                                                   cTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTaa                                                                                                                   >  2:732700/1‑93 (MQ=255)
                                                                                   cTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTaa                                                                                                                   <  1:732700/93‑1 (MQ=255)
                                                                                   cTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGt                                                           >  3:433213/1‑149 (MQ=255)
                                                                                   cTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGt                                                           >  4:341828/1‑149 (MQ=255)
                                                                                          aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                                    >  1:638762/1‑149 (MQ=255)
                                                                                          aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                                    >  2:221377/1‑149 (MQ=255)
                                                                                             gCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTc                                                 <  3:138698/149‑1 (MQ=255)
                                                                                              cTGCGCGAGAGTGTGGCGGAGTGACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCt                                                <  3:455239/149‑1 (MQ=255)
                                                                                              cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCt                                                <  1:193598/149‑1 (MQ=255)
                                                                                              cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCt                                                <  4:433213/149‑1 (MQ=255)
                                                                                              cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCt                                                <  2:572988/149‑1 (MQ=255)
                                                                                                             gCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa                                 >  2:177976/1‑149 (MQ=255)
                                                                                                                gAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt                              >  3:483147/1‑149 (MQ=255)
                                                                                                                       gAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCa                                    <  2:607135/136‑1 (MQ=255)
                                                                                                                       gAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCa                                    >  1:607135/1‑136 (MQ=255)
                                                                                                                            cGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCaa                  <  1:349492/149‑1 (MQ=255)
                                                                                                                             ggCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAAc                 >  1:304639/1‑149 (MQ=255)
                                                                                                                             ggCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAAc                 >  2:796639/1‑149 (MQ=255)
                                                                                                                             ggCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAAc                 <  1:764087/149‑1 (MQ=255)
                                                                                                                                cctgCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGTCGCAACGtc              >  3:154731/4‑149 (MQ=255)
                                                                                                                                   gCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc           <  3:503558/149‑1 (MQ=255)
                                                                                                                                   gCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc           <  3:376078/149‑1 (MQ=255)
                                                                                                                                      tgcgaaTGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTCCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGa        <  4:154731/144‑1 (MQ=255)
                                                                                                                                         gCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTc     <  1:221377/149‑1 (MQ=255)
                                                                                                                                         gCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTc     <  1:177976/149‑1 (MQ=255)
                                                                                                                                            tGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCCTCAGATTccac  <  3:642652/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
AGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCAC  >  NC_000913/2749943‑2750231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑