Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,835,936 A→G 6.1% I497T (ATT→ACT)  hypF ← carbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,835,9360AG 6.1% 211.3 / 3.7 66I497T (ATT→ACT) hypFcarbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base A (37/25);  new base G (2/2);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

CGGCTGCTTCGCTGCCAAATCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTA  >  NC_000913/2835788‑2836084
                                                                                                                                                    |                                                                                                                                                    
cGGCTGCTTCGCTGCCAAATCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCaa                                                                                                                                                      <  3:234082/149‑1 (MQ=255)
cGGCTGCTTCGCTGCCAAATCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCaa                                                                                                                                                      <  1:349521/149‑1 (MQ=255)
cGGCTGCTTCGCTGCCAAATCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCaa                                                                                                                                                      <  1:760915/149‑1 (MQ=255)
   ctgctTCGCTGCCAAATCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGa                                                                                                                                                   <  4:88596/149‑1 (MQ=255)
                 aaTCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATcc                                                                                                                                     <  4:75515/149‑1 (MQ=255)
                  aTCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCa                                                                                                                                    >  3:338436/1‑149 (MQ=255)
                     gCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCg                                                                                                                                 <  4:118275/149‑1 (MQ=255)
                         cccGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCa                                                                                                                             >  3:348134/1‑149 (MQ=255)
                          ccGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCAc                                                                                                                            <  3:108192/149‑1 (MQ=255)
                           cGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACt                                                                                                                           >  3:795225/1‑149 (MQ=255)
                             gAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGa                                                                                                                         >  4:11640/1‑149 (MQ=255)
                                  gcCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGctc                                                                                                                    >  1:195186/1‑149 (MQ=255)
                                    cACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTg                                                                                                                  >  4:167284/1‑149 (MQ=255)
                                    cACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTg                                                                                                                  <  2:294577/149‑1 (MQ=255)
                                    cACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTg                                                                                                                  >  3:523804/1‑149 (MQ=255)
                                          aGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGac                                                                                                            >  4:771750/1‑149 (MQ=255)
                                           ggCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGTCGCTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATTCCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGaca                                                                                                           >  4:491850/1‑149 (MQ=255)
                                           ggCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGaca                                                                                                           >  3:770952/1‑149 (MQ=255)
                                                        ggTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATgg                                                                                              <  1:618888/149‑1 (MQ=255)
                                                            cTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCat                                                                                          <  4:348134/149‑1 (MQ=255)
                                                             tCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCatg                                                                                         >  4:636339/1‑149 (MQ=255)
                                                              cacaTTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCatga                                                                                        <  3:167284/149‑1 (MQ=255)
                                                                  ttCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCatgatgat                                                                                    >  1:249080/1‑149 (MQ=255)
                                                                     gcgATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCa                                                                                 >  1:37874/1‑149 (MQ=255)
                                                                                 ccGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAGTGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCatga                                                                                        <  2:804331/130‑1 (MQ=255)
                                                                                 ccGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAGTGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCatga                                                                                        >  3:611215/1‑130 (MQ=255)
                                                                                 ccGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAGTGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCatga                                                                                        <  4:611215/130‑1 (MQ=255)
                                                                                 ccGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAGTGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCatga                                                                                        >  1:804331/1‑130 (MQ=255)
                                                                                 ccGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCg                                                                     >  4:112914/1‑149 (MQ=255)
                                                                                  cGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGt                                                                    <  4:795225/149‑1 (MQ=255)
                                                                                         aCTCGTCGCCCCACAAAGCGCCGTTCTCCCCCAGACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGtt                                                                         >  1:230299/1‑137 (MQ=255)
                                                                                         aCTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGtt                                                                         <  2:230299/137‑1 (MQ=255)
                                                                                         aCTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGa                                                             >  3:338081/1‑149 (MQ=255)
                                                                                              ccgccCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCAt                                                        <  4:523804/149‑1 (MQ=255)
                                                                                                  cccACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCa                                                    >  3:680567/1‑149 (MQ=255)
                                                                                                   ccACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCAc                                                   <  4:770952/149‑1 (MQ=255)
                                                                                                    cacaAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGTGGCCCCTGATGct                                                                                                                     >  3:775829/1‑82 (MQ=255)
                                                                                                    cacaAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGTGGCCACTGATgat                                                                                                                     <  4:775829/82‑3 (MQ=255)
                                                                                                    cacaAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCAcg                                                  <  1:125578/149‑1 (MQ=255)
                                                                                                       aaaGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGtt                                                                         >  4:62581/1‑123 (MQ=255)
                                                                                                       aaaGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGtt                                                                         <  3:62581/123‑1 (MQ=255)
                                                                                                       aaaGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACgcgc                                               >  3:62335/1‑149 (MQ=255)
                                                                                                       aaaGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACgcgc                                               >  4:698224/1‑149 (MQ=255)
                                                                                                            gcCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCAct                                          >  2:777329/1‑149 (MQ=255)
                                                                                                                   tcCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGga                                   <  3:636339/149‑1 (MQ=255)
                                                                                                                   tcCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGga                                   <  2:249080/149‑1 (MQ=255)
                                                                                                                     ccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGgaga                                 <  4:338081/149‑1 (MQ=255)
                                                                                                                     ccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGgaga                                 >  2:776207/1‑149 (MQ=255)
                                                                                                                     ccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGgaga                                 <  3:662621/149‑1 (MQ=255)
                                                                                                                     ccccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGgaga                                 <  2:37874/149‑1 (MQ=255)
                                                                                                                       ccATACCGATACCGTCGAGCGTCCAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGAAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACa                               >  2:550999/1‑149 (MQ=255)
                                                                                                                       ccATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACa                               >  2:135802/1‑149 (MQ=255)
                                                                                                                             cgataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGccc                         >  1:593513/1‑149 (MQ=255)
                                                                                                                             cgataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGccc                         >  3:302485/1‑149 (MQ=255)
                                                                                                                             cgataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGGACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGccc                         >  3:691088/1‑149 (MQ=255)
                                                                                                                               ataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGc                                                                      >  2:265868/1‑102 (MQ=255)
                                                                                                                               ataccgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGc                                                                      <  1:265868/102‑1 (MQ=255)
                                                                                                                                   cgTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATgc                   <  2:787027/149‑1 (MQ=255)
                                                                                                                                    gTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATgcg                  >  2:626840/1‑149 (MQ=255)
                                                                                                                                     tCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATgcgc                 >  4:239267/1‑149 (MQ=255)
                                                                                                                                        aGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTc              <  3:491850/149‑1 (MQ=255)
                                                                                                                                          cGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCAt            <  4:338436/149‑1 (MQ=255)
                                                                                                                                           gTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAg                            >  1:373320/1‑132 (MQ=255)
                                                                                                                                           gTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAg                            <  2:373320/132‑1 (MQ=255)
                                                                                                                                               aaGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcaca       >  2:421206/1‑149 (MQ=255)
                                                                                                                                               aaGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcaca       >  2:608128/1‑149 (MQ=255)
                                                                                                                                               aaGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGcaca       >  3:497206/1‑149 (MQ=255)
                                                                                                                                                aGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACaa      >  1:484983/1‑149 (MQ=255)
                                                                                                                                                aGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACaa      >  3:55476/1‑149 (MQ=255)
                                                                                                                                                    aTGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTa  <  1:136354/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGCTGCTTCGCTGCCAAATCGCCACCCGGAAGCGCCACTGCAGGCAAGCCGCCCAGGTGCTCACATTCGCGATAGTTCACCCGCAGGCACTCGCCGCCCCACAAAGCGCCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTA  >  NC_000913/2835788‑2836084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑