Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,105,056 G→A 5.1% S208S (TCG→TCA mltC → membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,105,0560GA 5.1% 266.9 / 3.3 79S208S (TCG→TCAmltCmembrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (35/40);  new base A (2/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

GAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCA  >  NC_000913/3104909‑3105204
                                                                                                                                                   |                                                                                                                                                    
gAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGc                                                                                                                                                     >  4:298347/1‑149 (MQ=255)
gAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGc                                                                                                                                                     <  1:44172/149‑1 (MQ=255)
 aaaaaCCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCt                                                                                                                                                    <  2:393129/149‑1 (MQ=255)
  aaaaCCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTg                                                                                                                                                   >  3:356191/1‑149 (MQ=255)
   aaaCCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGa                                                                                                                                                  >  3:140882/1‑149 (MQ=255)
         tCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTgg                                                                                                                                            >  3:676245/1‑149 (MQ=255)
           tGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCa                                                                                                                                          <  3:628633/149‑1 (MQ=255)
             aaGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCACTGATTCTGGCAAt                                                                                                                                        >  4:750254/1‑149 (MQ=255)
                  ccGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGc                                                                                                                                   >  1:466508/1‑149 (MQ=255)
                    gcagcaACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTAt                                                                                                                                     <  4:187157/145‑1 (MQ=255)
                    gcagcaACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTAt                                                                                                                                     >  3:187157/1‑145 (MQ=255)
                    gcagcaACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAg                                                                                                                                 >  4:648540/1‑149 (MQ=255)
                    gcagcaACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAg                                                                                                                                 >  1:377865/1‑149 (MQ=255)
                     cagcaACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGa                                                                                                                                <  2:211835/149‑1 (MQ=255)
                             ggCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCt                                                                                                                        <  2:228842/149‑1 (MQ=255)
                               cTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTc                                                                                                                      <  2:466508/149‑1 (MQ=255)
                               cTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTc                                                                                                                      <  4:158057/149‑1 (MQ=255)
                                tGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTcc                                                                                                                     <  2:377865/149‑1 (MQ=255)
                                tGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTcc                                                                                                                     <  3:648540/149‑1 (MQ=255)
                                  cGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCtt                                                                                                                   <  3:298347/149‑1 (MQ=255)
                                     atcatcTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGc                                                                                                                                                     >  3:91045/1‑112 (MQ=255)
                                     atcatcTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGc                                                                                                                                                     <  4:91045/112‑1 (MQ=255)
                                       catcTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAAcc                                                                                                              >  4:206652/1‑149 (MQ=255)
                                           tACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTa                                                                                                          >  3:606536/1‑149 (MQ=255)
                                            aCAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTAt                                                                                                         >  3:45000/1‑149 (MQ=255)
                                             cAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATg                                                                                                        >  4:280071/1‑149 (MQ=255)
                                             cAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATg                                                                                                        >  2:123669/1‑149 (MQ=255)
                                               gCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCg                                                                                                      <  4:676245/149‑1 (MQ=255)
                                                  tCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTc                                                                                                   <  4:537022/149‑1 (MQ=255)
                                                   cACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCa                                                                                                  <  3:729175/149‑1 (MQ=255)
                                                      cATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGcc                                                                                               >  4:170590/1‑149 (MQ=255)
                                                      cATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCACCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGcc                                                                                               >  3:715433/1‑149 (MQ=255)
                                                       aTTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCg                                                                                              >  3:748173/1‑149 (MQ=255)
                                                         tAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGCTTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGtt                                                                                            <  1:523882/149‑1 (MQ=255)
                                                         tAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGtt                                                                                            <  3:547776/149‑1 (MQ=255)
                                                          aaCATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTc                                                                                           <  4:356191/149‑1 (MQ=255)
                                                           aCATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTcg                                                                                          >  1:363250/1‑148 (MQ=255)
                                                           aCATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTAATGAGGCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCTGCCGTAcc                                                                                          >  2:182866/1‑149 (MQ=255)
                                                                  gCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATgcgc                                                                                   <  3:206652/149‑1 (MQ=255)
                                                                          accTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTa                                                                           >  4:245288/1‑149 (MQ=255)
                                                                           ccTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTaa                                                                          <  3:421013/149‑1 (MQ=255)
                                                                             ttGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATg                                                                        >  3:681408/1‑149 (MQ=255)
                                                                               gATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCa                                                                      <  4:681408/149‑1 (MQ=255)
                                                                                aTAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGCTTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAg                                                                     >  3:463967/1‑149 (MQ=255)
                                                                                 tAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAgg                                                                    <  4:748173/149‑1 (MQ=255)
                                                                                 tAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCACTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAgg                                                                    >  3:646023/1‑149 (MQ=255)
                                                                                    aCGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAggtgg                                                                 <  3:170590/149‑1 (MQ=255)
                                                                                     cGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAggtggt                                                                <  1:178484/149‑1 (MQ=255)
                                                                                     cGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAggtggt                                                                <  2:439853/149‑1 (MQ=255)
                                                                                      gTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTa                                                               >  4:294370/1‑149 (MQ=255)
                                                                                        gcgcACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTaca                                                             <  3:280071/149‑1 (MQ=255)
                                                                                          gcACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTcc                                                                                                                     <  3:484642/91‑1 (MQ=255)
                                                                                          gcACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTcc                                                                                                                     >  4:484642/1‑91 (MQ=255)
                                                                                          gcACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTacaac                                                           >  3:456210/1‑149 (MQ=255)
                                                                                            acaAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAatat                                                         >  2:473814/1‑149 (MQ=255)
                                                                                            acaAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACAt                                                         >  2:774121/1‑149 (MQ=255)
                                                                                            acaAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCACTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACAt                                                         <  4:646023/149‑1 (MQ=255)
                                                                                              aaaTATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATAc                                                       >  4:118217/1‑149 (MQ=255)
                                                                                                   tctcGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATGCTGCCg                                                  <  2:577982/149‑1 (MQ=255)
                                                                                                     tcGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCggg                                                <  3:391606/149‑1 (MQ=255)
                                                                                                              tCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGAtgtg                                       >  3:247497/1‑149 (MQ=255)
                                                                                                               ccGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGTTGGGATTAATGCAGATGGTATAACATTCTGCCGGGAAATATctgt                                      <  4:45000/149‑1 (MQ=255)
                                                                                                                cGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGtt                                     >  2:387382/1‑149 (MQ=255)
                                                                                                                        gTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTcc                                   <  2:334700/143‑1 (MQ=255)
                                                                                                                        gTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTcc                                   >  1:334700/1‑143 (MQ=255)
                                                                                                                            cGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAggg                         <  3:245288/149‑1 (MQ=255)
                                                                                                                             ggAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAgggg                        <  4:247497/149‑1 (MQ=255)
                                                                                                                             ggAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAgggg                        <  4:140882/149‑1 (MQ=255)
                                                                                                                                aaTATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGAAGGGGaac                     <  2:363250/149‑2 (MQ=255)
                                                                                                                                 atatGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTc                                                                                                                      >  3:624984/1‑51 (MQ=255)
                                                                                                                                 atatGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTc                                                                                                                      <  4:624984/51‑1 (MQ=255)
                                                                                                                                  tatGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATc                   <  1:123669/149‑1 (MQ=255)
                                                                                                                                    tGGCGTTGATGAGTCACTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCg                 <  3:750254/149‑1 (MQ=255)
                                                                                                                                      gCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGAtgtg                                       >  3:156026/1‑125 (MQ=255)
                                                                                                                                      gCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGAtgtg                                       <  4:156026/125‑1 (MQ=255)
                                                                                                                                       cGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCa              <  1:774121/149‑1 (MQ=255)
                                                                                                                                             tgaGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGa        >  4:184817/1‑149 (MQ=255)
                                                                                                                                                 tCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTggg                                                                               >  4:466133/1‑74 (MQ=255)
                                                                                                                                                 tCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTggg                                                                               <  3:466133/74‑1 (MQ=255)
                                                                                                                                                   gCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAggg                         >  2:797698/1‑126 (MQ=255)
                                                                                                                                                   gCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAggg                         <  1:797698/126‑1 (MQ=255)
                                                                                                                                                   gCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCa  >  2:407383/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCA  >  NC_000913/3104909‑3105204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: