Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,218,415 T→C 5.4% K221R (AAG→AGG)  aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,218,4150TC 5.4% 369.2 / 7.6 111K221R (AAG→AGG) aerfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base T (52/53);  new base C (3/3);  total (55/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 112 total aligned reads displayed.

CCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACA  >  NC_000913/3218268‑3218562
                                                                                                                                                   |                                                                                                                                                   
      cTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGc                                                                                                                                              <  2:180075/149‑1 (MQ=255)
       tGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGcc                                                                                                                                             <  2:237366/149‑1 (MQ=255)
       tGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGcc                                                                                                                                             >  1:355528/1‑149 (MQ=255)
       tGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGcc                                                                                                                                             >  1:509510/1‑149 (MQ=255)
        gACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCt                                                                                                                                            <  3:483172/149‑1 (MQ=255)
          cacTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGa                                                                                                                                          >  4:370697/1‑149 (MQ=255)
               gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGc                                                                                                                                     <  3:370697/149‑1 (MQ=255)
               gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGc                                                                                                                                     <  3:443629/149‑1 (MQ=255)
                acacCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                                                                                    <  3:348929/149‑1 (MQ=255)
                  acCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAAc                                                                                                                                  >  1:570315/1‑149 (MQ=255)
                    cTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACAt                                                                                                                                >  2:711844/1‑149 (MQ=255)
                       gCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACAtttt                                                                                                                             <  3:302380/149‑1 (MQ=255)
                       gCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACAtttt                                                                                                                             <  2:226521/149‑1 (MQ=255)
                       gCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACAtttt                                                                                                                             <  2:570315/149‑1 (MQ=255)
                        cTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTc                                                                                                                            <  2:542351/149‑1 (MQ=255)
                        cTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTc                                                                                                                            <  1:366967/149‑1 (MQ=255)
                            agaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATc                                                                                                                        <  2:509510/149‑1 (MQ=255)
                            agaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATc                                                                                                                        >  1:484126/1‑149 (MQ=255)
                                aTCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgc                                                                                                                    >  4:395339/1‑149 (MQ=255)
                                aTCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgc                                                                                                                    >  4:609018/1‑149 (MQ=255)
                                aTCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACATAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgc                                                                                                                    <  3:585971/149‑1 (MQ=255)
                                   gatAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCt                                                                                                                                            >  3:291509/3‑122 (MQ=255)
                                   gTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCt                                                                                                                                            <  4:291509/122‑1 (MQ=255)
                                   gTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGca                                                                                                                 >  2:508957/1‑149 (MQ=255)
                                        ttAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATc                                                                                                            <  3:552686/149‑1 (MQ=255)
                                        ttAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATc                                                                                                            <  1:711844/149‑1 (MQ=255)
                                            ccAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGcc                                                                                                        >  3:703148/1‑149 (MQ=255)
                                                cGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTc                                                                                                    >  2:131578/1‑149 (MQ=255)
                                                  gCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCaa                                                                                                  <  2:673332/149‑1 (MQ=255)
                                                    acaTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCaaaa                                                                                                >  2:103604/1‑149 (MQ=255)
                                                    acaTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCaaaa                                                                                                <  3:743846/149‑1 (MQ=255)
                                                      aTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAAc                                                                                                                                  <  4:317022/113‑1 (MQ=255)
                                                      aTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAAc                                                                                                                                  >  3:317022/1‑113 (MQ=255)
                                                      aTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAAc                                                                                                                                  >  3:68618/1‑113 (MQ=255)
                                                      aTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACa                                                                                              >  3:753504/1‑149 (MQ=255)
                                                      aTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACa                                                                                              >  3:237527/1‑149 (MQ=255)
                                                      aTCAGGCCAAGTTGCCCTACCACACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAAc                                                                                                                                  <  4:68618/113‑1 (MQ=255)
                                                       tCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAg                                                                                             <  3:395339/149‑1 (MQ=255)
                                                       tCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAg                                                                                             >  1:224747/1‑149 (MQ=255)
                                                             cAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCtt                                                                                       <  4:225454/149‑1 (MQ=255)
                                                             cAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCtt                                                                                       <  2:355528/149‑1 (MQ=255)
                                                             cAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCtt                                                                                       <  2:352388/149‑1 (MQ=255)
                                                             cAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCtt                                                                                       <  2:543962/149‑1 (MQ=255)
                                                             cAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCtt                                                                                       >  1:315932/1‑149 (MQ=255)
                                                             cAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCtt                                                                                       >  4:735276/1‑149 (MQ=255)
                                                               aGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGc                                                                                     <  2:224747/149‑1 (MQ=255)
                                                               aGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGc                                                                                     <  2:484126/149‑1 (MQ=255)
                                                                  tGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCcaa                                                                                  <  4:703148/149‑1 (MQ=255)
                                                                       tACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCCACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAAGAGGCGCTTGCCAACACTa                                                                             <  4:535476/149‑1 (MQ=255)
                                                                        aCCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCCTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATAc                                                                            <  3:330307/149‑1 (MQ=255)
                                                                           gCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATAGCAc                                                                         <  1:700869/149‑1 (MQ=255)
                                                                             aCGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTa                                                                       <  1:103604/149‑1 (MQ=255)
                                                                              cGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTc                                                                                                                                                   <  1:473722/72‑1 (MQ=255)
                                                                                tAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACg                                                                    >  1:609778/1‑149 (MQ=255)
                                                                                tAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACg                                                                    >  4:160481/1‑149 (MQ=255)
                                                                                  aTGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGca                                                                  <  1:42709/149‑1 (MQ=255)
                                                                                     tCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACag                                                               >  2:316447/1‑149 (MQ=255)
                                                                                         ccccAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGa                                                           <  3:189247/149‑1 (MQ=255)
                                                                                          cccAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCaaaa                                                                                                <  3:537526/111‑1 (MQ=255)
                                                                                           ccAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGata                                                         >  2:297020/1‑149 (MQ=255)
                                                                                            cAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGatat                                                        <  1:508957/149‑1 (MQ=255)
                                                                                            cAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGatat                                                        >  4:245789/1‑149 (MQ=255)
                                                                                                tCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGt                                                    <  3:160481/149‑1 (MQ=255)
                                                                                                       tGCGATTCAGATGCTCAACACTATCACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCaccacc                                             >  1:293424/1‑149 (MQ=255)
                                                                                                           aTTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCgggg                                         >  3:117837/1‑149 (MQ=255)
                                                                                                            ttCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGgc                                        >  4:312504/1‑149 (MQ=255)
                                                                                                               aGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGcaca                                                                <  1:485631/122‑1 (MQ=255)
                                                                                                               aGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGcaca                                                                >  2:485631/1‑122 (MQ=255)
                                                                                                                gATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGgcagca                                    >  4:162733/1‑149 (MQ=255)
                                                                                                                 aTGCTCAACACTATTACGTTCTCCGGTCGCCACCCTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAGACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGcaa                                   <  3:98329/149‑1 (MQ=255)
                                                                                                                 aTGCTCAACACTATTACGTTCTCCGGTCGCCACCCTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGcaa                                   <  1:190526/149‑1 (MQ=255)
                                                                                                                   gCTCAACACTATTACGTTCTCCGGTCGCCACCTACAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGcaaca                                 >  3:506021/1‑149 (MQ=255)
                                                                                                                     tCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCAt                                                                                                      >  3:60758/1‑78 (MQ=255)
                                                                                                                     tCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCAt                                                                                                      <  4:60758/78‑1 (MQ=255)
                                                                                                                     tCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACaaa                               <  2:704898/149‑1 (MQ=255)
                                                                                                                       aaCACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCcaa                                                                                  <  4:394619/96‑1 (MQ=255)
                                                                                                                       aaCACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACag                                                               >  2:99430/1‑115 (MQ=255)
                                                                                                                       aaCACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACag                                                               <  1:99430/115‑1 (MQ=255)
                                                                                                                       aaCACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGat                                                          >  3:662640/1‑120 (MQ=255)
                                                                                                                       aaCACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGat                                                          <  4:662640/120‑1 (MQ=255)
                                                                                                                       aaCACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAAcc                             >  3:617463/1‑149 (MQ=255)
                                                                                                                         cacTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcgc                                                                                                                  <  2:103207/62‑1 (MQ=255)
                                                                                                                         cacTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcgc                                                                                                                  >  1:103207/1‑62 (MQ=255)
                                                                                                                          acTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCaaa                          >  1:496911/1‑149 (MQ=255)
                                                                                                                            tATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGcaac                                  <  2:425956/139‑1 (MQ=255)
                                                                                                                            tATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGc                        >  2:660114/1‑149 (MQ=255)
                                                                                                                            tATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGc                        >  3:466664/1‑149 (MQ=255)
                                                                                                                              ttACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATAc                                                      >  2:235283/1‑117 (MQ=255)
                                                                                                                              ttACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATAc                                                      <  1:235283/117‑1 (MQ=255)
                                                                                                                              ttACGTTCTCCGGTCGACACCTTCAGTGCCTGATGGGCAGCATTTTCTATCGGGCGCACAATCTGCCTTTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGCCAGCAACAAACCAAAGCAt                      >  2:547656/1‑149 (MQ=255)
                                                                                                                               tACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATCTACGTCACCACCGGGGCAGCAACAAACCAAAGCATg                     <  4:759032/149‑1 (MQ=255)
                                                                                                                               tACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATg                     <  4:286348/149‑1 (MQ=255)
                                                                                                                                  gTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGgcc                  <  3:245789/149‑1 (MQ=255)
                                                                                                                                      tcCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCaaa                          >  4:379252/1‑137 (MQ=255)
                                                                                                                                      tcCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCaaa                          <  3:379252/137‑1 (MQ=255)
                                                                                                                                       ccGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAg             <  4:753504/149‑1 (MQ=255)
                                                                                                                                             gCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGAt       >  4:45954/1‑149 (MQ=255)
                                                                                                                                             gCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACCTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGAt       <  4:466664/149‑1 (MQ=255)
                                                                                                                                                aCCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATaaa    >  3:45650/1‑149 (MQ=255)
                                                                                                                                                  cTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACa  >  4:411721/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACA  >  NC_000913/3218268‑3218562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: